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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: kitten_wizard] * 1
    #23424459 - 07/08/16 06:49 PM (7 years, 11 months ago)

this is the direct section from which I derived this recipe, originally from hardydiagnostics.com, a supplier of microbiology media and lab supplies.

Quote:

Potato Flake Agar, based on the formula recommended by M.G. Rinaldi, is designed to contain the proper formulation of carbon, protein and nutrient sources recommended for the identification of mold fungi encountered in the clinical mycology laboratory. (6) Glucose is added to the medium to provide a carbon and energy source. The media also contains potato flakes which promote conidiation by molds. The pH is adjusted to approximately 5.6 in order to enhance the growth of fungi and to slightly inhibit bacterial growth commonly found as contaminants. (1-4,6) In addition, selective agents are added to increase the inhibitory properties of Potato Flake Selective Agar to inhibit growth of gram-positive and gram-negative bacteria.

FORMULA

Ingredients per liter of deionized water:*

Potato Flakes 20.0gm
Glucose 10.0gm
Agar 15.0gm





so obv I'm not adjusting the pH or adding selective agents. and I also adjusted the ratios for slightly more agar for good tiger dropping, and more potato flakes because their ratio was causing very strange strained growth in my cultures. I added more flakes and my cultures went back to normal.

also I've had plenty of pooling and condensation and its never given any problems. just ignore it.

I've worked with this recipe for 1.5 years before I published it, so give it another whirl. 1/2 a petri in a week sounds awesome, I'm not sure what you consider standard but, as you can see above, that is pretty good by what experienced growers here have seen.

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InvisibleInocuole
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Re: Agar as easy as 1-2-3! [Re: Munchauzen] * 1
    #23424512 - 07/08/16 07:08 PM (7 years, 11 months ago)

Almost thinking that was less of a "I need help with this" and more of a "negative customer review" type post. :lol:


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OfflineSloppyJoseph
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Re: Agar as easy as 1-2-3! [Re: Inocuole]
    #23424515 - 07/08/16 07:09 PM (7 years, 11 months ago)

:sogood:


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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: SloppyJoseph] * 1
    #23424523 - 07/08/16 07:13 PM (7 years, 11 months ago)

:cookiemonster:

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InvisibleMad Season
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Re: Agar as easy as 1-2-3! [Re: kitten_wizard] * 2
    #23424831 - 07/08/16 09:18 PM (7 years, 11 months ago)

Quote:

kitten_wizard said:
I used this recipe for my first ever agar and i have to say I am not very happy with it.

So far the growth is unbearably slow with them at 74f. A week and it's not even half filled the petri. There is also a decent amount of pooling on the gel as well as considerable condensation that doesn't seem to want to go away even with trying a warm cup on top of the dish.

I think I'm gonna try something else.



Lol what?? Dude that is what I am for. Half a dish in 1 week. 2-3 weeks and it's overgrown.


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Invisiblemupetmower
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Re: Agar as easy as 1-2-3! [Re: Mad Season]
    #23425777 - 07/09/16 07:08 AM (7 years, 11 months ago)

He has never done this before? Yet he thinks he knows how things need to be. Gah, people can be so ignorant.


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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: mupetmower]
    #23425817 - 07/09/16 07:34 AM (7 years, 11 months ago)

Quote:

mupetmower said:
He has never done this before? Yet he thinks he knows how things need to be. Gah, people can be so ignorant.




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Invisiblemupetmower
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23426202 - 07/09/16 10:54 AM (7 years, 11 months ago)

Heh, just to be clear, I meant ignorant in its literal meaning. Which is along the lines of "not having any knowledge of what they are taking about" :thumbup:

So not as an insult :wink:


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: mupetmower] * 1
    #23440744 - 07/14/16 12:32 PM (7 years, 11 months ago)

:bump2:

added a link to pasty plates at the beginning of the write-up so I dont get so many :pm: asking what to do next :lol:

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Offlineludka1
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23886916 - 12/02/16 09:02 AM (7 years, 6 months ago)

Sorry, but what are pasty plates? By the looks of them, they look like standard Tupperware, but I'm not really sure. I didn't think that Tupperware could withstand pcing..

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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: ludka1]
    #23887122 - 12/02/16 10:22 AM (7 years, 6 months ago)


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InvisibleInocuole
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Re: Agar as easy as 1-2-3! [Re: Munchauzen] * 1
    #23888817 - 12/02/16 08:20 PM (7 years, 6 months ago)

You have to consider that if said tupperware couldn't be PC'd, then there wouldn't be a huge thread by a TC about PCing tupperware.  So, it's kind of one of those "can you believe how hot it is today?" questions.  "Yes, I can believe it".


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InvisibleComebackKid
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23904135 - 12/07/16 04:13 PM (7 years, 6 months ago)

I just understood the title of this post while measuring out the ingredients...:facepalm3: :lol:

Do you get nice rhyzo growth with this recipe?


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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: ComebackKid]
    #23904519 - 12/07/16 06:16 PM (7 years, 6 months ago)

Quote:

ComebackKid said:
I just understood the title of this post while measuring out the ingredients...:facepalm3: :lol:

Do you get nice rhyzo growth with this recipe?




it does well. you can always reduce the sugar and potato if its too rich for your culture.


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InvisibleComebackKid
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23904594 - 12/07/16 06:37 PM (7 years, 6 months ago)

Just whipped up a batch. That pic shows basically what I'm dealing with right now. Gunna try playing with the recipe a bit but I'm definitely moving on from the drop of honey and onto measuring sugar on my scale :thumbup:


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
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Our individual experiences are all part of the universe's experience of itself

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Invisiblewolfedawwg
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Re: Agar as easy as 1-2-3! [Re: ComebackKid]
    #23904698 - 12/07/16 07:03 PM (7 years, 6 months ago)

:takingnotes: 
Thanks!!


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Offlinekeifnnugs
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Re: Agar as easy as 1-2-3! [Re: wolfedawwg]
    #23904884 - 12/07/16 07:46 PM (7 years, 6 months ago)

Been looking for a new recipe.. Thanks Munch!

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Re: Agar as easy as 1-2-3! [Re: Munchauzen] * 1
    #23926068 - 12/14/16 01:35 PM (7 years, 6 months ago)

Quote:

Munchauzen said:
Potato Dextrose Agar & Pasty Plate Preperation

for use with: Pastywhyte's Easy Agar Tek

For 8-10 Pasty Plates You Will Need:
- 1 gram honey or raw sugar
- 2 grams agar
- 3 grams potato flakes
- 1/2 cup water
- optional: food coloring

Directions:
- Set your stove-top to high, then combine all ingredients in a pot. Use the hot water to help dissolve the honey.
- Let the mixture reach a boil
- Upon boiling, immediately remove and pour into a measuring cup. You can use the the cup to see how close to 1/2 cup you are. if you cooked off too much water, you can simply add some back in.
- Pour into your pasty plates and set the lid on to keep debris out.
- Let them cool down, then wrap in foil and PC 30 minutes at 15psi.



Notes:
I've adjusted the agar amount in this recipe from a previous recipe, in order to accomodate for my Blenderless Liquid Inoculate tek. This is the recipe I use for that tek, and it works great. The plates aren't mushy, but just slightly softer, which breaks apart nicely with the LI tek, but also behaves normally for transfers.






I'll just bump this for anyone using 100mm petri dishes this recipe will get you about 5 dishes so double if wanting 8-10.


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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: Dabrit]
    #23926307 - 12/14/16 02:42 PM (7 years, 6 months ago)

Thanks :thumbup:

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InvisibleComebackKid
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Re: Agar as easy as 1-2-3! [Re: Munchauzen] * 1
    #23988848 - 01/07/17 09:11 AM (7 years, 5 months ago)

This recipe kicks ass
Before this I was always checking my phone for the recipe. Now it's as easy as 1 2 3
And if I want less nutes I just take a half gram of sugar and a half gram of potato out of the recipe.
:rockon:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself

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