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InvisibleMunchauzen
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Agar as easy as 123! * 29
    #23188000 - 05/04/16 09:11 AM (8 years, 1 month ago)

Potato Dextrose Agar & Pasty Plate Preperation

for use with: Pastywhyte's Easy Agar Tek

For 8-10 PDA Pasty Plates You Will Need:
- 1 gram honey or raw sugar
- 2 grams agar
- 3 grams potato flakes (plain instant mashed potatos)
- 1/2 cup water
- optional: food coloring

Directions:
- Set your stove-top to high, then combine all ingredients in a pot. Use the hot water to help dissolve the honey.
- Let the mixture reach a boil
- Upon boiling, immediately remove and pour into a measuring cup. You can use the the cup to see how close to 1/2 cup you are. if you cooked off too much water, you can simply add some back in.
- Pour into your pasty plates and set the lid on to keep debris out.
- Let them cool down, then wrap in foil and PC 30 minutes at 15psi.




Notes:
I've adjusted the agar amount in this recipe from a previous recipe, in order to accomodate for my Blenderless Liquid Inoculate tek. This is the recipe I use for that tek, and it works great. The plates aren't mushy, but just slightly softer, which breaks apart nicely with the LI tek, but also behaves normally for transfers.

Additionally, here is my recipe for LME:
- 2 grams light malt extract
- 2 grams agar
- 1/2 cup water
- optional: food coloring

Edited by Munchauzen (03/06/24 06:55 PM)

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InvisibleJosex
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23188008 - 05/04/16 09:13 AM (8 years, 1 month ago)

I just wanted to be the first madafaka posting :crondance:

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InvisiblePastywhyteMDiscord
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Re: Agar as easy as 1-2-3! [Re: Josex] * 1
    #23188014 - 05/04/16 09:15 AM (8 years, 1 month ago)

Nice munch!

:manofapproval:

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Invisiblemupetmower
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Re: Agar as easy as 1-2-3! [Re: Munchauzen] * 1
    #23188015 - 05/04/16 09:15 AM (8 years, 1 month ago)

thanks Munch! this is essentially how i do it. although, i feel like i have been using more agar than potato flakes. gonna try it this exact way next time.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

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Invisibleoontribe
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Re: Agar as easy as 1-2-3! [Re: mupetmower]
    #23188021 - 05/04/16 09:17 AM (8 years, 1 month ago)

sweet!

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OfflinePsilosoulful

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Re: Agar as easy as 1-2-3! [Re: oontribe]
    #23188040 - 05/04/16 09:23 AM (8 years, 1 month ago)

:woot:

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Offlinetripdawg420
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Re: Agar as easy as 1-2-3! [Re: Psilosoulful]
    #23188210 - 05/04/16 10:26 AM (8 years, 1 month ago)

:takingnotes:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Invisibledankington
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Re: Agar as easy as 1-2-3! [Re: tripdawg420]
    #23188249 - 05/04/16 10:39 AM (8 years, 1 month ago)

:rockon: Thanks for this, Munch!

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Offlinetombosley8
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Re: Agar as easy as 1-2-3! [Re: dankington]
    #23188532 - 05/04/16 12:25 PM (8 years, 1 month ago)

after you let the agar cool does it solidify before pcing?

I thought I was heating the liquid up too much until I saw this vid, thanks

Is it necessary/beneficial for it to boil and/or solidify?


--------------------

Edited by tombosley8 (05/04/16 12:26 PM)

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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: tombosley8] * 1
    #23188538 - 05/04/16 12:26 PM (8 years, 1 month ago)

Quote:

tombosley8 said:
after you let the agar cool do they solidify before pcing?

I thought I was heating the liquid up too much until I saw this vid, thanks

Is it necessary/beneficial for it to boil and/or solidify?



I let it solidify so I don't spill any or get the filter wet when I wrap it with foil

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InvisibleJosex
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23191893 - 05/05/16 11:01 AM (8 years, 1 month ago)

Munch can you tell me exactly how many millimeters is a 1/2 cup of water? We don't have those measuring cups where I live.
My guess is that for this recipe you use 15 grams of agar for a litre of water, is that correct? I've been having troubles with my agar being too stiff for this.

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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: Josex] * 1
    #23191923 - 05/05/16 11:07 AM (8 years, 1 month ago)

120 milliliters

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InvisibleJosex
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23191929 - 05/05/16 11:09 AM (8 years, 1 month ago)

Sweet thanks!

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Offlinegoofypov
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Re: Agar as easy as 1-2-3! [Re: Josex]
    #23191947 - 05/05/16 11:15 AM (8 years, 1 month ago)

nice, gonna try this ratio.

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OfflineFeedYourMind
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Re: Agar as easy as 1-2-3! [Re: goofypov]
    #23192031 - 05/05/16 11:43 AM (8 years, 1 month ago)

Nice, I'll give this a shot my next round. :headbanger:


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InvisibleNeomorph
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Re: Agar as easy as 1-2-3! [Re: FeedYourMind]
    #23192284 - 05/05/16 01:04 PM (8 years, 1 month ago)

Thanks man, we needed this one :yesnod:

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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: Neomorph]
    #23193332 - 05/05/16 06:57 PM (8 years, 1 month ago)

:thumbup: thanks guys

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Offlinekitten_wizard
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23424311 - 07/08/16 05:48 PM (7 years, 11 months ago)

I used this recipe for my first ever agar and i have to say I am not very happy with it.

So far the growth is unbearably slow with them at 74f. A week and it's not even half filled the petri. There is also a decent amount of pooling on the gel as well as considerable condensation that doesn't seem to want to go away even with trying a warm cup on top of the dish.

I think I'm gonna try something else.


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Invisibledankington
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Re: Agar as easy as 1-2-3! [Re: kitten_wizard] * 1
    #23424316 - 07/08/16 05:51 PM (7 years, 11 months ago)

Half grown in a week from spores? Doesn't sound bad to me at all...

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InvisibleInocuole
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Re: Agar as easy as 1-2-3! [Re: kitten_wizard] * 1
    #23424318 - 07/08/16 05:52 PM (7 years, 11 months ago)

I think you'll find that if this is your first agar you may not have anything to compare it to.  If you're using actual petris then that could have a whole lot more to do with it.  And seriously, half grown in a week is pretty good.


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