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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Agar as easy as 123! 29
#23188000 - 05/04/16 09:11 AM (8 years, 1 month ago) |
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Potato Dextrose Agar & Pasty Plate Preperation
for use with: Pastywhyte's Easy Agar Tek
For 8-10 PDA Pasty Plates You Will Need: - 1 gram honey or raw sugar - 2 grams agar - 3 grams potato flakes (plain instant mashed potatos) - 1/2 cup water - optional: food coloring
Directions: - Set your stove-top to high, then combine all ingredients in a pot. Use the hot water to help dissolve the honey. - Let the mixture reach a boil - Upon boiling, immediately remove and pour into a measuring cup. You can use the the cup to see how close to 1/2 cup you are. if you cooked off too much water, you can simply add some back in. - Pour into your pasty plates and set the lid on to keep debris out. - Let them cool down, then wrap in foil and PC 30 minutes at 15psi.
Notes: I've adjusted the agar amount in this recipe from a previous recipe, in order to accomodate for my Blenderless Liquid Inoculate tek. This is the recipe I use for that tek, and it works great. The plates aren't mushy, but just slightly softer, which breaks apart nicely with the LI tek, but also behaves normally for transfers.
Additionally, here is my recipe for LME: - 2 grams light malt extract - 2 grams agar - 1/2 cup water - optional: food coloring
Edited by Munchauzen (03/06/24 06:55 PM)
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,998
Loc:
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#23188008 - 05/04/16 09:13 AM (8 years, 1 month ago) |
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I just wanted to be the first madafaka posting
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,885
Loc: Canada
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Re: Agar as easy as 1-2-3! [Re: Josex] 1
#23188014 - 05/04/16 09:15 AM (8 years, 1 month ago) |
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Nice munch!
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Agar as easy as 1-2-3! [Re: Munchauzen] 1
#23188015 - 05/04/16 09:15 AM (8 years, 1 month ago) |
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thanks Munch! this is essentially how i do it. although, i feel like i have been using more agar than potato flakes. gonna try it this exact way next time.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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oontribe


Registered: 01/14/15
Posts: 3,570
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Re: Agar as easy as 1-2-3! [Re: mupetmower]
#23188021 - 05/04/16 09:17 AM (8 years, 1 month ago) |
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sweet!
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 15 days, 9 hours
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Re: Agar as easy as 1-2-3! [Re: oontribe]
#23188040 - 05/04/16 09:23 AM (8 years, 1 month ago) |
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,619
Loc: illinois
Last seen: 3 hours, 1 minute
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Thanks for this, Munch!
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 1 year, 1 month
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Re: Agar as easy as 1-2-3! [Re: dankington]
#23188532 - 05/04/16 12:25 PM (8 years, 1 month ago) |
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after you let the agar cool does it solidify before pcing?
I thought I was heating the liquid up too much until I saw this vid, thanks
Is it necessary/beneficial for it to boil and/or solidify?
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Edited by tombosley8 (05/04/16 12:26 PM)
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: Agar as easy as 1-2-3! [Re: tombosley8] 1
#23188538 - 05/04/16 12:26 PM (8 years, 1 month ago) |
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Quote:
tombosley8 said: after you let the agar cool do they solidify before pcing?
I thought I was heating the liquid up too much until I saw this vid, thanks
Is it necessary/beneficial for it to boil and/or solidify?
I let it solidify so I don't spill any or get the filter wet when I wrap it with foil
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,998
Loc:
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#23191893 - 05/05/16 11:01 AM (8 years, 1 month ago) |
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Munch can you tell me exactly how many millimeters is a 1/2 cup of water? We don't have those measuring cups where I live. My guess is that for this recipe you use 15 grams of agar for a litre of water, is that correct? I've been having troubles with my agar being too stiff for this.
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: Agar as easy as 1-2-3! [Re: Josex] 1
#23191923 - 05/05/16 11:07 AM (8 years, 1 month ago) |
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120 milliliters
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,998
Loc:
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#23191929 - 05/05/16 11:09 AM (8 years, 1 month ago) |
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Sweet thanks!
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goofypov
Stranger
Registered: 01/07/16
Posts: 7
Last seen: 7 years, 3 months
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Re: Agar as easy as 1-2-3! [Re: Josex]
#23191947 - 05/05/16 11:15 AM (8 years, 1 month ago) |
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nice, gonna try this ratio.
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FeedYourMind
indiGlo



Registered: 03/17/14
Posts: 1,155
Loc: That One Place
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Re: Agar as easy as 1-2-3! [Re: goofypov]
#23192031 - 05/05/16 11:43 AM (8 years, 1 month ago) |
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Nice, I'll give this a shot my next round.
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Neomorph


Registered: 12/10/14
Posts: 767
Loc: Europe
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Thanks man, we needed this one
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: Agar as easy as 1-2-3! [Re: Neomorph]
#23193332 - 05/05/16 06:57 PM (8 years, 1 month ago) |
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thanks guys
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kitten_wizard
Registered: 12/07/13
Posts: 115
Last seen: 10 months, 6 days
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#23424311 - 07/08/16 05:48 PM (7 years, 11 months ago) |
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I used this recipe for my first ever agar and i have to say I am not very happy with it.
So far the growth is unbearably slow with them at 74f. A week and it's not even half filled the petri. There is also a decent amount of pooling on the gel as well as considerable condensation that doesn't seem to want to go away even with trying a warm cup on top of the dish.
I think I'm gonna try something else.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Half grown in a week from spores? Doesn't sound bad to me at all...
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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I think you'll find that if this is your first agar you may not have anything to compare it to. If you're using actual petris then that could have a whole lot more to do with it. And seriously, half grown in a week is pretty good.
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