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InvisibleMunchauzen
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Agar as easy as 123! * 28
    #23188000 - 05/04/16 09:11 AM (7 years, 9 months ago)

Philly Golden Teacher can go fuck himself.

Potato Dextrose Agar & Pasty Plate Preperation

for use with: Pastywhyte's Easy Agar Tek

For 8-10 PDA Pasty Plates You Will Need:
- 1 gram honey or raw sugar
- 2 grams agar
- 3 grams potato flakes (plain instant mashed potatos)
- 1/2 cup water
- optional: food coloring

Directions:
- Set your stove-top to high, then combine all ingredients in a pot. Use the hot water to help dissolve the honey.
- Let the mixture reach a boil
- Upon boiling, immediately remove and pour into a measuring cup. You can use the the cup to see how close to 1/2 cup you are. if you cooked off too much water, you can simply add some back in.
- Pour into your pasty plates and set the lid on to keep debris out.
- Let them cool down, then wrap in foil and PC 30 minutes at 15psi.




Notes:
I've adjusted the agar amount in this recipe from a previous recipe, in order to accomodate for my Blenderless Liquid Inoculate tek. This is the recipe I use for that tek, and it works great. The plates aren't mushy, but just slightly softer, which breaks apart nicely with the LI tek, but also behaves normally for transfers.

Additionally, here is my recipe for LME:
- 2 grams light malt extract
- 2 grams agar
- 1/2 cup water
- optional: food coloring


Edited by Munchauzen (12/05/23 07:38 PM)


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InvisibleJosex
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23188008 - 05/04/16 09:13 AM (7 years, 9 months ago)

I just wanted to be the first madafaka posting :crondance:


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InvisiblePastywhyteMDiscord
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Re: Agar as easy as 1-2-3! [Re: Josex] * 1
    #23188014 - 05/04/16 09:15 AM (7 years, 9 months ago)

Nice munch!

:manofapproval:


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Invisiblemupetmower
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Re: Agar as easy as 1-2-3! [Re: Munchauzen] * 1
    #23188015 - 05/04/16 09:15 AM (7 years, 9 months ago)

thanks Munch! this is essentially how i do it. although, i feel like i have been using more agar than potato flakes. gonna try it this exact way next time.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Invisibleoontribe
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Re: Agar as easy as 1-2-3! [Re: mupetmower]
    #23188021 - 05/04/16 09:17 AM (7 years, 9 months ago)

sweet!


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OfflinePsilosoulful

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Re: Agar as easy as 1-2-3! [Re: oontribe]
    #23188040 - 05/04/16 09:23 AM (7 years, 9 months ago)

:woot:


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Offlinetripdawg420
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Re: Agar as easy as 1-2-3! [Re: Psilosoulful]
    #23188210 - 05/04/16 10:26 AM (7 years, 9 months ago)

:takingnotes:


--------------------
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results :thumbup:


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Invisibledankington
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Re: Agar as easy as 1-2-3! [Re: tripdawg420]
    #23188249 - 05/04/16 10:39 AM (7 years, 9 months ago)

:rockon: Thanks for this, Munch!


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Offlinetombosley8
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Re: Agar as easy as 1-2-3! [Re: dankington]
    #23188532 - 05/04/16 12:25 PM (7 years, 9 months ago)

after you let the agar cool does it solidify before pcing?

I thought I was heating the liquid up too much until I saw this vid, thanks

Is it necessary/beneficial for it to boil and/or solidify?


--------------------


Edited by tombosley8 (05/04/16 12:26 PM)


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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: tombosley8] * 1
    #23188538 - 05/04/16 12:26 PM (7 years, 9 months ago)

Quote:

tombosley8 said:
after you let the agar cool do they solidify before pcing?

I thought I was heating the liquid up too much until I saw this vid, thanks

Is it necessary/beneficial for it to boil and/or solidify?



I let it solidify so I don't spill any or get the filter wet when I wrap it with foil


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InvisibleJosex
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23191893 - 05/05/16 11:01 AM (7 years, 9 months ago)

Munch can you tell me exactly how many millimeters is a 1/2 cup of water? We don't have those measuring cups where I live.
My guess is that for this recipe you use 15 grams of agar for a litre of water, is that correct? I've been having troubles with my agar being too stiff for this.


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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: Josex] * 1
    #23191923 - 05/05/16 11:07 AM (7 years, 9 months ago)

120 milliliters


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InvisibleJosex
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23191929 - 05/05/16 11:09 AM (7 years, 9 months ago)

Sweet thanks!


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Offlinegoofypov
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Re: Agar as easy as 1-2-3! [Re: Josex]
    #23191947 - 05/05/16 11:15 AM (7 years, 9 months ago)

nice, gonna try this ratio.


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OfflineFeedYourMind
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Re: Agar as easy as 1-2-3! [Re: goofypov]
    #23192031 - 05/05/16 11:43 AM (7 years, 9 months ago)

Nice, I'll give this a shot my next round. :headbanger:


--------------------


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Re: Agar as easy as 1-2-3! [Re: FeedYourMind]
    #23192284 - 05/05/16 01:04 PM (7 years, 9 months ago)

Thanks man, we needed this one :yesnod:


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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: Neomorph]
    #23193332 - 05/05/16 06:57 PM (7 years, 9 months ago)

:thumbup: thanks guys


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23424311 - 07/08/16 05:48 PM (7 years, 7 months ago)

I used this recipe for my first ever agar and i have to say I am not very happy with it.

So far the growth is unbearably slow with them at 74f. A week and it's not even half filled the petri. There is also a decent amount of pooling on the gel as well as considerable condensation that doesn't seem to want to go away even with trying a warm cup on top of the dish.

I think I'm gonna try something else.


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Invisibledankington
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Re: Agar as easy as 1-2-3! [Re: kitten_wizard] * 1
    #23424316 - 07/08/16 05:51 PM (7 years, 7 months ago)

Half grown in a week from spores? Doesn't sound bad to me at all...


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InvisibleInocuole
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Re: Agar as easy as 1-2-3! [Re: kitten_wizard] * 1
    #23424318 - 07/08/16 05:52 PM (7 years, 7 months ago)

I think you'll find that if this is your first agar you may not have anything to compare it to.  If you're using actual petris then that could have a whole lot more to do with it.  And seriously, half grown in a week is pretty good.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: kitten_wizard] * 1
    #23424459 - 07/08/16 06:49 PM (7 years, 7 months ago)

this is the direct section from which I derived this recipe, originally from hardydiagnostics.com, a supplier of microbiology media and lab supplies.

Quote:

Potato Flake Agar, based on the formula recommended by M.G. Rinaldi, is designed to contain the proper formulation of carbon, protein and nutrient sources recommended for the identification of mold fungi encountered in the clinical mycology laboratory. (6) Glucose is added to the medium to provide a carbon and energy source. The media also contains potato flakes which promote conidiation by molds. The pH is adjusted to approximately 5.6 in order to enhance the growth of fungi and to slightly inhibit bacterial growth commonly found as contaminants. (1-4,6) In addition, selective agents are added to increase the inhibitory properties of Potato Flake Selective Agar to inhibit growth of gram-positive and gram-negative bacteria.

FORMULA

Ingredients per liter of deionized water:*

Potato Flakes 20.0gm
Glucose 10.0gm
Agar 15.0gm





so obv I'm not adjusting the pH or adding selective agents. and I also adjusted the ratios for slightly more agar for good tiger dropping, and more potato flakes because their ratio was causing very strange strained growth in my cultures. I added more flakes and my cultures went back to normal.

also I've had plenty of pooling and condensation and its never given any problems. just ignore it.

I've worked with this recipe for 1.5 years before I published it, so give it another whirl. 1/2 a petri in a week sounds awesome, I'm not sure what you consider standard but, as you can see above, that is pretty good by what experienced growers here have seen.


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InvisibleInocuole
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Re: Agar as easy as 1-2-3! [Re: Munchauzen] * 1
    #23424512 - 07/08/16 07:08 PM (7 years, 7 months ago)

Almost thinking that was less of a "I need help with this" and more of a "negative customer review" type post. :lol:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineSloppyJoseph
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Re: Agar as easy as 1-2-3! [Re: Inocuole]
    #23424515 - 07/08/16 07:09 PM (7 years, 7 months ago)

:sogood:


--------------------
AMU Q&A


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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: SloppyJoseph] * 1
    #23424523 - 07/08/16 07:13 PM (7 years, 7 months ago)

:cookiemonster:


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InvisibleMad Season
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Re: Agar as easy as 1-2-3! [Re: kitten_wizard] * 2
    #23424831 - 07/08/16 09:18 PM (7 years, 7 months ago)

Quote:

kitten_wizard said:
I used this recipe for my first ever agar and i have to say I am not very happy with it.

So far the growth is unbearably slow with them at 74f. A week and it's not even half filled the petri. There is also a decent amount of pooling on the gel as well as considerable condensation that doesn't seem to want to go away even with trying a warm cup on top of the dish.

I think I'm gonna try something else.



Lol what?? Dude that is what I am for. Half a dish in 1 week. 2-3 weeks and it's overgrown.


--------------------
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Invisiblemupetmower
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Re: Agar as easy as 1-2-3! [Re: Mad Season]
    #23425777 - 07/09/16 07:08 AM (7 years, 7 months ago)

He has never done this before? Yet he thinks he knows how things need to be. Gah, people can be so ignorant.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: mupetmower]
    #23425817 - 07/09/16 07:34 AM (7 years, 7 months ago)

Quote:

mupetmower said:
He has never done this before? Yet he thinks he knows how things need to be. Gah, people can be so ignorant.





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Invisiblemupetmower
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23426202 - 07/09/16 10:54 AM (7 years, 7 months ago)

Heh, just to be clear, I meant ignorant in its literal meaning. Which is along the lines of "not having any knowledge of what they are taking about" :thumbup:

So not as an insult :wink:


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: mupetmower] * 1
    #23440744 - 07/14/16 12:32 PM (7 years, 7 months ago)

:bump2:

added a link to pasty plates at the beginning of the write-up so I dont get so many :pm: asking what to do next :lol:


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23886916 - 12/02/16 09:02 AM (7 years, 2 months ago)

Sorry, but what are pasty plates? By the looks of them, they look like standard Tupperware, but I'm not really sure. I didn't think that Tupperware could withstand pcing..


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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: ludka1]
    #23887122 - 12/02/16 10:22 AM (7 years, 2 months ago)



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InvisibleInocuole
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Re: Agar as easy as 1-2-3! [Re: Munchauzen] * 1
    #23888817 - 12/02/16 08:20 PM (7 years, 2 months ago)

You have to consider that if said tupperware couldn't be PC'd, then there wouldn't be a huge thread by a TC about PCing tupperware.  So, it's kind of one of those "can you believe how hot it is today?" questions.  "Yes, I can believe it".


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleComebackKid
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23904135 - 12/07/16 04:13 PM (7 years, 2 months ago)

I just understood the title of this post while measuring out the ingredients...:facepalm3: :lol:

Do you get nice rhyzo growth with this recipe?


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: ComebackKid]
    #23904519 - 12/07/16 06:16 PM (7 years, 2 months ago)

Quote:

ComebackKid said:
I just understood the title of this post while measuring out the ingredients...:facepalm3: :lol:

Do you get nice rhyzo growth with this recipe?




it does well. you can always reduce the sugar and potato if its too rich for your culture.



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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #23904594 - 12/07/16 06:37 PM (7 years, 2 months ago)

Just whipped up a batch. That pic shows basically what I'm dealing with right now. Gunna try playing with the recipe a bit but I'm definitely moving on from the drop of honey and onto measuring sugar on my scale :thumbup:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Re: Agar as easy as 1-2-3! [Re: ComebackKid]
    #23904698 - 12/07/16 07:03 PM (7 years, 2 months ago)

:takingnotes: 
Thanks!!


--------------------
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Offlinekeifnnugs
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Re: Agar as easy as 1-2-3! [Re: wolfedawwg]
    #23904884 - 12/07/16 07:46 PM (7 years, 2 months ago)

Been looking for a new recipe.. Thanks Munch!


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Re: Agar as easy as 1-2-3! [Re: Munchauzen] * 1
    #23926068 - 12/14/16 01:35 PM (7 years, 2 months ago)

Quote:

Munchauzen said:
Potato Dextrose Agar & Pasty Plate Preperation

for use with: Pastywhyte's Easy Agar Tek

For 8-10 Pasty Plates You Will Need:
- 1 gram honey or raw sugar
- 2 grams agar
- 3 grams potato flakes
- 1/2 cup water
- optional: food coloring

Directions:
- Set your stove-top to high, then combine all ingredients in a pot. Use the hot water to help dissolve the honey.
- Let the mixture reach a boil
- Upon boiling, immediately remove and pour into a measuring cup. You can use the the cup to see how close to 1/2 cup you are. if you cooked off too much water, you can simply add some back in.
- Pour into your pasty plates and set the lid on to keep debris out.
- Let them cool down, then wrap in foil and PC 30 minutes at 15psi.



Notes:
I've adjusted the agar amount in this recipe from a previous recipe, in order to accomodate for my Blenderless Liquid Inoculate tek. This is the recipe I use for that tek, and it works great. The plates aren't mushy, but just slightly softer, which breaks apart nicely with the LI tek, but also behaves normally for transfers.






I'll just bump this for anyone using 100mm petri dishes this recipe will get you about 5 dishes so double if wanting 8-10.


--------------------
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InvisibleMunchauzen
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Re: Agar as easy as 1-2-3! [Re: Dabrit]
    #23926307 - 12/14/16 02:42 PM (7 years, 2 months ago)

Thanks :thumbup:


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InvisibleComebackKid
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Re: Agar as easy as 1-2-3! [Re: Munchauzen] * 1
    #23988848 - 01/07/17 09:11 AM (7 years, 1 month ago)

This recipe kicks ass
Before this I was always checking my phone for the recipe. Now it's as easy as 1 2 3
And if I want less nutes I just take a half gram of sugar and a half gram of potato out of the recipe.
:rockon:


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Re: Agar as easy as 1-2-3! [Re: ComebackKid]
    #23988929 - 01/07/17 09:33 AM (7 years, 1 month ago)

precisely. i may be a stoner, but after a year of mixture agar i was still fucking up the recipe from time to time. this one is pretty difficult not to remember


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24011509 - 01/15/17 08:44 AM (7 years, 1 month ago)

:bump2:


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OfflinePsilosoulful

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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24013717 - 01/16/17 03:03 AM (7 years, 1 month ago)

:thumbup:


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Offlinesynthetik
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Re: Agar as easy as 1-2-3! [Re: Psilosoulful]
    #24013829 - 01/16/17 06:20 AM (7 years, 1 month ago)

took me too long to realize the 1-2-3 aspect of this...  :facepalm3:

also s.a.p. for the order... 123 sap 1/2c. h20 too easy!

thanks again and also to those posting about using less nutes... just wondering do you guys use less nutes when you see slow growth? just wondering when or why you would do that, thanks!


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Re: Agar as easy as 1-2-3! [Re: synthetik]
    #24019629 - 01/18/17 10:46 AM (7 years, 1 month ago)

I love the simplicity of blenderless LI and want to give it a go, but I currently have Light Malt Extract on hand, not potato flakes.

For LME, would I just need to omit the honey and convert this to a "2-3" (2g Agar - 3g LME to half-cup h2o) mix for the same softer consistency as the 1-2-3 PDA?


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Re: Agar as easy as 1-2-3! [Re: pheasant327]
    #24019851 - 01/18/17 12:35 PM (7 years, 1 month ago)

Quote:

pheasant327 said:
I love the simplicity of blenderless LI and want to give it a go, but I currently have Light Malt Extract on hand, not potato flakes.

For LME, would I just need to omit the honey and convert this to a "2-3" (2g Agar - 3g LME to half-cup h2o) mix for the same softer consistency as the 1-2-3 PDA?




I would just do 2-2.5 grams malt myself.


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Re: Agar as easy as 1-2-3! [Re: Pastywhyte]
    #24019966 - 01/18/17 01:16 PM (7 years, 1 month ago)



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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24019996 - 01/18/17 01:29 PM (7 years, 1 month ago)

I made these but replaced the potato flakes with BRF.

The recipe made 9 pasty plates for me but one of them is 1/10th of a mm thick.


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Re: Agar as easy as 1-2-3! [Re: Tiamo]
    #24020002 - 01/18/17 01:32 PM (7 years, 1 month ago)

would that explain some of your issues??? or does it grow fine?


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Re: Agar as easy as 1-2-3! [Re: mushboy]
    #24020007 - 01/18/17 01:33 PM (7 years, 1 month ago)

I haven't tried any pasty plates yet. I'm gonna drop spores tomorrow, been putting it off. They look real good. Most of them are thick as agar in a petri would be.


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Re: Agar as easy as 1-2-3! [Re: Tiamo]
    #24020011 - 01/18/17 01:35 PM (7 years, 1 month ago)

:awethumb:


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Re: Agar as easy as 1-2-3! [Re: mushboy]
    #24020049 - 01/18/17 01:47 PM (7 years, 1 month ago)

Quote:


For LME, would I just need to omit the honey and convert this to a "2-3" (2g Agar - 3g LME to half-cup h2o) mix for the same softer consistency as the 1-2-3 PDA?




The MEA recipe I like to use is:
10g (extra) light malt extract
10g agar
500ml distilled water



It gives you a pretty clear media. You can dye it of course..


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Re: Agar as easy as 1-2-3! [Re: enlightenment]
    #24020182 - 01/18/17 02:28 PM (7 years, 1 month ago)

Pasty and Enlightenment - Thanks guys. I have been using 8.5g-10g LME/10g Agar/500mL for the majority of my plates up to this point, just wasn't sure if it would be soft enough to break up well while doing the blenderless shake.

13shrooms - I have yet to try LC, but I plan to give it a go in the coming weeks.


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Re: Agar as easy as 1-2-3! [Re: pheasant327]
    #24037226 - 01/24/17 08:38 PM (7 years, 29 days ago)

:bump2:


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24051785 - 01/30/17 01:35 PM (7 years, 24 days ago)

I just tried this recipe the other day, I was using the recipe in pasty's tek but I wanted a softer plate for LI. I was using a tsp of powdered agar, thinking that was about 2.5 grams. Well I weighed my ingredients to be more precise and a tsp of powdered agar was just under 2gs. What I'm curious about is are you using powdered or the chopped up sticks? I'm using telephone brand. Kinda sucks because 1-1.5-3 isnt as easy to remember...


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Re: Agar as easy as 1-2-3! [Re: mushboy]
    #24051798 - 01/30/17 01:41 PM (7 years, 24 days ago)

Grew fine :rockon:


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Re: Agar as easy as 1-2-3! [Re: Tiamo]
    #24051813 - 01/30/17 01:49 PM (7 years, 24 days ago)

I use powdered. Also have flakes sitting around that i havnt used since i got the powder


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24114142 - 02/23/17 06:00 PM (6 years, 11 months ago)

Seems like a good PDA recipe, will come back to it when I'm bored of MEA :smile:


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Re: Agar as easy as 1-2-3! [Re: lovelaughlibs]
    #24203958 - 03/29/17 09:39 PM (6 years, 10 months ago)

:bump2:


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24318811 - 05/14/17 12:31 PM (6 years, 9 months ago)

:bump2:


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24319440 - 05/14/17 06:28 PM (6 years, 9 months ago)

:rockon:


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24532421 - 08/05/17 12:48 PM (6 years, 6 months ago)

posting in here so I can find it easily!


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Re: Agar as easy as 1-2-3! *DELETED* [Re: PreparationH]
    #24594918 - 08/31/17 11:54 PM (6 years, 5 months ago)

Enjoy


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Gratitude increases the value of what we already have


Edited by miketheexplorer (09/01/17 10:34 AM)


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Re: Agar as easy as 1-2-3! [Re: miketheexplorer]
    #24594926 - 09/01/17 12:00 AM (6 years, 5 months ago)

Quote:

pierre2008 said:
I'm diving in and trying out I'm gonna give Pastywhyte's Easy Agar!!

I have 2 questions, I'm stuck on:
1. Do I need to drill the hole in the Glad minis or do I cover, wrap in foil and put in PC?

2. How much water should I put into the pressure cooker?  The minis are very small


Thank you




1. Yes they will be crushed otherwise
2. You should put the minimum amount that your pressure cooker requires as stated in the manual. I put two layers of mason jar lids in the bottom and then the trivet/rack that came with the pressure. I fill the water to the trivet which is a few inches of water. The plates go on top of that.

Those questions make it seem like you haven't read the pasty plate thread closely or are ignoring what it says for some reason. You might want to read it again.


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Re: Agar as easy as 1-2-3! *DELETED* [Re: senseit]
    #24594987 - 09/01/17 01:34 AM (6 years, 5 months ago)

Post deleted by pierre2008

Reason for deletion: not interested in the thread



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Gratitude increases the value of what we already have


Edited by miketheexplorer (09/01/17 01:56 AM)


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Re: Agar as easy as 1-2-3! [Re: miketheexplorer]
    #24595145 - 09/01/17 05:29 AM (6 years, 5 months ago)

I drill well actually melt mine in the side with a soldering iron the cover with mp tape. I'm using 2 oz plastic shot cups from dollar tree with lids. Do around 27 a pop in a 23 qt presto. I have a lot of agar going, I mean alot, just started 7 weeks ago on t8 now


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Re: Agar as easy as 1-2-3! [Re: miketheexplorer] * 1
    #24595326 - 09/01/17 07:42 AM (6 years, 5 months ago)

Quote:

pierre2008 said:
Quote:

senseit said:
Quote:

pierre2008 said:
I'm diving in and trying out I'm gonna give Pastywhyte's Easy Agar!!

I have 2 questions, I'm stuck on:
1. Do I need to drill the hole in the Glad minis or do I cover, wrap in foil and put in PC?

2. How much water should I put into the pressure cooker?  The minis are very small


Thank you




1. Yes they will be crushed otherwise
2. You should put the minimum amount that your pressure cooker requires as stated in the manual. I put two layers of mason jar lids in the bottom and then the trivet/rack that came with the pressure. I fill the water to the trivet which is a few inches of water. The plates go on top of that.

Those questions make it seem like you haven't read the pasty plate thread closely or are ignoring what it says for some reason. You might want to read it again.





Thank you appreciate the information in your response. 

Don't appreciate your ignorant comment at the end. You've been here a little over a year, and I've been here over 7 ... if you can't say anything kind, censor yourself for the sake of everyone's sanity




I don't see his comment as ignorant. Just an honest opinion and it was good of him to mention it. Criticism is not easy to recieve but it's critical to success in life. This hobby can be challenging and if you can't handle some frank comments it's going to be even harder when you get struck with some real challenges. You are growing a felony, imagine how sensitive the authorities will be if you get caught.

Length of tenure has very little to do with skill set. You have been here longer than I have. What does that say?


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24595363 - 09/01/17 08:08 AM (6 years, 5 months ago)

:justastonishing:


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Re: Agar as easy as 1-2-3! [Re: muchlove]
    #24595400 - 09/01/17 08:26 AM (6 years, 5 months ago)

Am I the only one who finds it completely impossible to make PastyPlates with no condensation? I've made a few batches now, and I don't get what I'm doing wrong. I'm about to make 16 plates tonight, and would like to get them out of the pc with no water on the sides. I always let the pc cool way longer than 8 hours. Usually closer to 20/24 hours by the time I'm able to take everything out. Advice?


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Re: Agar as easy as 1-2-3! [Re: dhype773]
    #24595407 - 09/01/17 08:33 AM (6 years, 5 months ago)

Same here, visibility is kinda impaired, it's largely why I did some pour petris.


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Re: Agar as easy as 1-2-3! [Re: lovelaughlibs]
    #24595413 - 09/01/17 08:37 AM (6 years, 5 months ago)

Condensation is pretty much a non issue for me. Usually dries up in a few days. It's not a vector. Make your plates in advance.


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Re: Agar as easy as 1-2-3! [Re: Pastywhyte]
    #24595452 - 09/01/17 08:54 AM (6 years, 5 months ago)

I do,  I have them in the fridge, but it never clears up, even after a month. But I guess that's because I leave the foil on until I use them


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Re: Agar as easy as 1-2-3! [Re: dhype773]
    #24595492 - 09/01/17 09:12 AM (6 years, 5 months ago)

Take the foil off and it clears fast. I leave it on until I use them and they clear in a few days, usually in time to observe the growth. Also never store unused plates in the fridge, if there are CFU'S present you want them to germ and grow so you know not to use them.


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Re: Agar as easy as 1-2-3! [Re: Pastywhyte]
    #24595542 - 09/01/17 09:36 AM (6 years, 5 months ago)

Thanks man!


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Re: Agar as easy as 1-2-3! [Re: Pastywhyte]
    #24595657 - 09/01/17 10:33 AM (6 years, 5 months ago)

I am not discussing tenure as it relates to skill set, so not sure where you got that from. 
An encouraging attitude is everything in sharing knowledge, especially in an forum where everyone is trying to improve.


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Re: Agar as easy as 1-2-3! [Re: miketheexplorer]
    #24595784 - 09/01/17 11:17 AM (6 years, 5 months ago)

Tenure came in when you compared your reg date to his. As for encouragement I feel that people demand too much. It wasn't like he called you a fucking tard and a waste of skin. He just said that it felt like you weren't absorbing as much as you should have and that you should reread. The fact that he took the time to try and help you improve should be enough. Nothing to be butthurt over IMO.

I don't sugar coat my advice, I simply don't get paid enough to do so. Take what I give you or leave it, it's your grow not mine.

:twocents:


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Re: Agar as easy as 1-2-3! [Re: Pastywhyte]
    #24595802 - 09/01/17 11:23 AM (6 years, 5 months ago)

I do the same. Take the foil off. Let em sit a few days and the condensation is gone.


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Re: Agar as easy as 1-2-3! [Re: Pastywhyte]
    #24595826 - 09/01/17 11:33 AM (6 years, 5 months ago)

They way we do and say things is everything in terms of results, personal and professional. 

Leaving it ... Got 100 petri dishes ordered
Enjoy the ride
1 cent :-)


--------------------
Gratitude increases the value of what we already have


Edited by miketheexplorer (09/01/17 11:40 AM)


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Re: Agar as easy as 1-2-3! [Re: miketheexplorer]
    #24595876 - 09/01/17 12:02 PM (6 years, 5 months ago)

So the only way you are willing to accept advice or criticism is if the person giving you the help is simultaneously kissing your ass? Seems to me that it should be the other way around.

:goodluckwiththat:


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Re: Agar as easy as 1-2-3! [Re: eatyualive]
    #24596036 - 09/01/17 01:07 PM (6 years, 5 months ago)

Quote:

eatyualive said:
I do the same. Take the foil off. Let em sit a few days and the condensation is gone.



Environmental conditions come into play when it comes to the condensation of all media vessels.  If your temps swing 10-15 degrees, you will see condensation.  The best fix for condensation is to keep your spawning area at a constant temperature and relative humidity.  I go with 70-72F & around 50% RH.


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Re: Agar as easy as 1-2-3! *DELETED* [Re: miketheexplorer] * 1
    #24596124 - 09/01/17 02:04 PM (6 years, 5 months ago)

Quote:

pierre2008 said:
Post deleted by pierre2008<p>Reason for deletion: not interested in the thread



lol


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24596234 - 09/01/17 02:50 PM (6 years, 5 months ago)

Can't wait to try this out
:mushroom2:


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Re: Agar as easy as 1-2-3! [Re: Fungeyes] * 1
    #24596806 - 09/01/17 07:11 PM (6 years, 5 months ago)

Munch,
Thank you for you tutorial videos
I learn a lot from them.


Edited by miketheexplorer (09/01/17 07:25 PM)


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Re: Agar as easy as 1-2-3! [Re: miketheexplorer]
    #24598355 - 09/02/17 12:10 PM (6 years, 5 months ago)

Quote:

pierre2008 said:
They way we do and say things is everything in terms of results, personal and professional. 

Leaving it ... Got 100 petri dishes ordered
Enjoy the ride
1 cent :-)




You'll still need to know how to run your pressure cooker properly with petri dishes. I was concerned by your question that you would run the cooker with too little water. According to what I've read, running a pressure cooker dry weakens it and is possibly dangerous. I learned a lot from watching Bod's Easy AF how to use your pressure cooker video, like how to keep my jars from getting mineral buildup and how to run the pressure cooker with less noise. Even if you know how to run the pressure cooker, you still might learn stuff you didn't know. :thumbup:


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Re: Agar as easy as 1-2-3! [Re: hamloaf]
    #24598464 - 09/02/17 01:15 PM (6 years, 5 months ago)

Love this clear, simple recipe and the video that goes with it. Thanks! The flakes are so much nicer to work with than LME.


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Re: Agar as easy as 1-2-3! [Re: apollo321]
    #24599274 - 09/02/17 08:37 PM (6 years, 5 months ago)

Quote:

senseit said:
Quote:

pierre2008 said:
They way we do and say things is everything in terms of results, personal and professional. 

Leaving it ... Got 100 petri dishes ordered
Enjoy the ride
1 cent :-)




You'll still need to know how to run your pressure cooker properly with petri dishes. I was concerned by your question that you would run the cooker with too little water. According to what I've read, running a pressure cooker dry weakens it and is possibly dangerous. I learned a lot from watching Bod's Easy AF how to use your pressure cooker video, like how to keep my jars from getting mineral buildup and how to run the pressure cooker with less noise. Even if you know how to run the pressure cooker, you still might learn stuff you didn't know. :thumbup:




Agree, been doing research on agar for several weeks, seen Bods vids several times, there's also many good videos on youtube
Got the petri dishes today
Bought the pressure cooker today, clear operating instructions
Made the still box
Made the Malt agar ... like the color red for good contrast
Sometimes you just have to say fuck it and do it
Easy and quite fun ... think i'll be doing this a lot more lol
cheers mate!



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Edited by miketheexplorer (09/02/17 08:43 PM)


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Re: Agar as easy as 1-2-3! [Re: miketheexplorer]
    #24599299 - 09/02/17 08:54 PM (6 years, 5 months ago)

another piece of advice, remove the lid from your still air box. just wipe off your surface there and work on that. its a lot easier to work on a stable surface than a plastic lid.



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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24599399 - 09/02/17 10:08 PM (6 years, 5 months ago)

Munch, you literally read my mind!
I was wondering if I could remove the lid from the SAB,  the plates were sliding all over the place

Thank you!!! Really appreciate it  :bow2:


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Re: Agar as easy as 1-2-3! [Re: miketheexplorer]
    #24599538 - 09/02/17 11:58 PM (6 years, 5 months ago)

:popcorn:


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Offlinemiketheexplorer
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24599565 - 09/03/17 12:29 AM (6 years, 5 months ago)

Quote:

Munchauzen said:
another piece of advice, remove the lid from your still air box. just wipe off your surface there and work on that. its a lot easier to work on a stable surface than a plastic lid.






Munch, is just saw your video on working with a SAB.  I think you spray soapy water or ISO 70, then cover the SAB. 

I just saw this, of a guy putting all his stuff inside the SAB and then spraying with disinfectant ... does this work well?



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Re: Agar as easy as 1-2-3! [Re: miketheexplorer]
    #24599741 - 09/03/17 03:03 AM (6 years, 5 months ago)

its soapy water. never spray isopropyl alcohol in a sab. it effectively creates a bomb.

and thats a big fuck no to spraying disinfectant in your sab. still air and a peaceful soul is all you need to do sterile agar work.


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24599742 - 09/03/17 03:06 AM (6 years, 5 months ago)

:takingnotes:

:rockon:


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Re: Agar as easy as 1-2-3! [Re: miketheexplorer]
    #24600063 - 09/03/17 09:16 AM (6 years, 5 months ago)

Quote:

pierre2008 said:





:justno:


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Re: Agar as easy as 1-2-3! [Re: bodhisatta]
    #24604470 - 09/04/17 10:20 PM (6 years, 5 months ago)

tried this the other day and everything looking good so far.
very easy to remember
cheers munch


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Re: Agar as easy as 1-2-3! [Re: bill bixby]
    #24604512 - 09/04/17 10:37 PM (6 years, 5 months ago)

:ohyeah:


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24605368 - 09/05/17 10:23 AM (6 years, 5 months ago)

i use your softer recipe alot man, thanks


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Re: Agar as easy as 1-2-3! [Re: miketheexplorer]
    #24609579 - 09/06/17 06:33 PM (6 years, 5 months ago)

Quote:

pierre2008 said:
Quote:

senseit said:
Quote:

pierre2008 said:
They way we do and say things is everything in terms of results, personal and professional. 

Leaving it ... Got 100 petri dishes ordered
Enjoy the ride
1 cent :-)




You'll still need to know how to run your pressure cooker properly with petri dishes. I was concerned by your question that you would run the cooker with too little water. According to what I've read, running a pressure cooker dry weakens it and is possibly dangerous. I learned a lot from watching Bod's Easy AF how to use your pressure cooker video, like how to keep my jars from getting mineral buildup and how to run the pressure cooker with less noise. Even if you know how to run the pressure cooker, you still might learn stuff you didn't know. :thumbup:




Agree, been doing research on agar for several weeks, seen Bods vids several times, there's also many good videos on youtube
Got the petri dishes today
Bought the pressure cooker today, clear operating instructions
Made the still box
Made the Malt agar ... like the color red for good contrast
Sometimes you just have to say fuck it and do it
Easy and quite fun ... think i'll be doing this a lot more lol
cheers mate!







Hey Munch,

I've also been trying to step up to the world of agar and have watched the videos and built my now 3rd SAB after not taking the time to properly choose one that is the right size THE FIRST TIME.

Since I forgot to make spore prints the last few grows I did I had to buy another couple spore syringes. Considering that this wont be a tiger drop, would it be wise to drop the powdered agar quantity just a tad?

I also picked up an inoculation loop set. Is this even worth the hassle this round since I'm coming from a syringe?

I'm super stoked to try this. I'll be holed up the house this weekend for the hurricane and can't think of a better time to bite the agar bullet. I have finally overcame the first couple grow jitters and want to make sure I do everything the right way this time.


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Re: Agar as easy as 1-2-3! [Re: jeezy117]
    #24609609 - 09/06/17 06:41 PM (6 years, 5 months ago)

no need to change the recipe. it works great for spores and transfers alike.

no need for a loop with the syringe. just heat up the dispensing needle to red hot, then make a little slice thru the middle of the agar to make a little valley and drop your solution into that.


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24615889 - 09/09/17 11:58 AM (6 years, 5 months ago)

first time with agar, ive 15 plates and in 10minutes it'll have been 30mins after they were set do i then place in pressure cooker for 30mins and leave out on the side for next day spore transfer ?


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Re: Agar as easy as 1-2-3! [Re: Cujllickduo]
    #24615939 - 09/09/17 12:21 PM (6 years, 5 months ago)

yes you need to pressure cook them for 30 minutes. after that, they are good to use for quite some time.


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24616238 - 09/09/17 02:47 PM (6 years, 5 months ago)

Just did my first plates..i guess the pc shifted to one side when they were setting cus a few had alot more on one side than the other. Hope they still work. Did 8 with a ms syringe. Will see what happens in a week..i know i def need more practice.


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Re: Agar as easy as 1-2-3! [Re: Variegated]
    #24616252 - 09/09/17 02:51 PM (6 years, 5 months ago)

I want to get some agar powder and i was wondering which shroomery vendor has the best deal for around $20  shipping.


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Re: Agar as easy as 1-2-3! [Re: Variegated]
    #24616302 - 09/09/17 03:15 PM (6 years, 5 months ago)

Quote:

Variegated said:
Just did my first plates..i guess the pc shifted to one side when they were setting cus a few had alot more on one side than the other. Hope they still work. Did 8 with a ms syringe. Will see what happens in a week..i know i def need more practice.



they will still work but you dont have to keep it like that. toss em back in the pc heat em up until they melt back to liquid, then take them out and set them on the counter while they cool down. dont have to bring up to pressure, just a short steam is enough to remelt it.


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24616359 - 09/09/17 03:36 PM (6 years, 5 months ago)

There noced up now will do better when i make more to transfer. Didnt get much boil up but did have lots of condensation on this first run


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Re: Agar as easy as 1-2-3! [Re: Variegated]
    #24618775 - 09/10/17 04:48 PM (6 years, 5 months ago)

Quick question. About to cook this recipe up. Do you guys sterilize your tools while pcing agar or just wipe down with 70% iso  and flame b4 transfers?


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Re: Agar as easy as 1-2-3! [Re: JHOVA]
    #24618780 - 09/10/17 04:51 PM (6 years, 5 months ago)

Quote:

Jonhova said:
I want to get some agar powder and i was wondering which shroomery vendor has the best deal for around $20  shipping.




Picked mine up at the store for $2 25g telephone agar agar


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Re: Agar as easy as 1-2-3! [Re: Fungeyes]
    #24619876 - 09/11/17 05:32 AM (6 years, 5 months ago)

Quote:

Fungeyes said:
Quick question. About to cook this recipe up. Do you guys sterilize your tools while pcing agar or just wipe down with 70% iso  and flame b4 transfers?



I wipe the full tool including handle down with IPA then flame just the blade. Never had any issues.


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Re: Agar as easy as 1-2-3! [Re: Fungeyes]
    #24619880 - 09/11/17 05:36 AM (6 years, 5 months ago)

Quote:

Fungeyes said:
Quick question. About to cook this recipe up. Do you guys sterilize your tools while pcing agar or just wipe down with 70% iso  and flame b4 transfers?




Ate you asking if we sterilize the things used to make the agar?


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Re: Agar as easy as 1-2-3! [Re: Fungeyes]
    #24620292 - 09/11/17 09:49 AM (6 years, 5 months ago)

Quote:

Fungeyes said:
Quick question. About to cook this recipe up. Do you guys sterilize your tools while pcing agar or just wipe down with 70% iso  and flame b4 transfers?



yes. wipe the handle with 70% iso. avoid getting iso on the blade so when you flame it, the whole thing doesnt go up in flames. i usually iso my handle every 2-3 dishes.


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24620297 - 09/11/17 09:51 AM (6 years, 5 months ago)

:ohyeah:


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24655584 - 09/24/17 10:18 AM (6 years, 4 months ago)

Quote:

Munchauzen said:
no need to change the recipe. it works great for spores and transfers alike.

no need for a loop with the syringe. just heat up the dispensing needle to red hot, then make a little slice thru the middle of the agar to make a little valley and drop your solution into that.




Munch,

I can slice through the agar with the red hot needle right? This would cool down the needle enough to allow the MS solution to colonize?

Or do I need to use a sterilized x-acto knife?


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Re: Agar as easy as 1-2-3! [Re: jeezy117]
    #24655627 - 09/24/17 10:31 AM (6 years, 4 months ago)

use the syringe


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24655687 - 09/24/17 10:52 AM (6 years, 4 months ago)

Copy that.

Thanks for the info.

On a side note, which I'm sure you have heard 1000 times: The Randy Marshes make me loose me shit every time. It's like the icing on the cake to the good info.


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Re: Agar as easy as 1-2-3! [Re: jeezy117]
    #24655752 - 09/24/17 11:19 AM (6 years, 4 months ago)



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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #24656121 - 09/24/17 01:23 PM (6 years, 4 months ago)

:hypnotoad:


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Re: Agar as easy as 1-2-3! [Re: Cujllickduo]
    #25047566 - 03/07/18 10:41 PM (5 years, 11 months ago)

the video has been re-uploaded!



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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #25103485 - 03/31/18 12:02 AM (5 years, 10 months ago)

:takingnotes:


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Re: Agar as easy as 1-2-3! [Re: Inocuole]
    #25557710 - 10/22/18 12:00 PM (5 years, 4 months ago)

How much growth on a mini round is acceptable to transfer from?


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Re: Agar as easy as 1-2-3! [Re: thelanzii]
    #25557739 - 10/22/18 12:12 PM (5 years, 4 months ago)

dime sized and on


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Re: Agar as easy as 1-2-3! [Re: Variegated]
    #25819057 - 02/17/19 02:44 PM (5 years, 6 days ago)

Does anybody know after Pressure cooking the pastey plates do you have to apply a new layer of micropore tape each time? Or can you reuse?


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Re: Agar as easy as 1-2-3! [Re: Dabrit]
    #25819176 - 02/17/19 03:48 PM (5 years, 6 days ago)

reuse is fine


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #26257922 - 10/17/19 08:33 AM (4 years, 4 months ago)

plates wont melt in PC?


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Re: Agar as easy as 1-2-3! [Re: Samalander]
    #26257930 - 10/17/19 08:36 AM (4 years, 4 months ago)

Quote:

Samalander said:
plates wont melt in PC?




this tek is using PP5 grade plastic no pour plates
not standard petries

pastyplates - no pour agar


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Re: Agar as easy as 1-2-3! [Re: Camera93]
    #26257939 - 10/17/19 08:39 AM (4 years, 4 months ago)

ok so I remove that problem by not PC ing them, and pouring agar in a SAB?


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Re: Agar as easy as 1-2-3! [Re: Samalander]
    #26257946 - 10/17/19 08:42 AM (4 years, 4 months ago)

yes I know this is not tek for petris, I both small plastic boxes that I found and Im not sure will that plastic withstand temperatures in PC


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Re: Agar as easy as 1-2-3! [Re: Samalander]
    #26257957 - 10/17/19 08:48 AM (4 years, 4 months ago)

Quote:

Samalander said:
ok so I remove that problem by not PC ing them, and pouring agar in a SAB?




For pour agar yeah, but the receiving plate has to be sterile. like a sleeve of petrie dishes. this wont work for normal plastic containers

Quote:

Samalander said:
yes I know this is not tek for petris, I both small plastic boxes that I found and Im not sure will that plastic withstand temperatures in PC




I depends on what grade of plastic it is. PP5 can withstand the PC
your dish outta be stamped with a little triangle and number
can you spot it?


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Re: Agar as easy as 1-2-3! [Re: Camera93]
    #26257967 - 10/17/19 08:51 AM (4 years, 4 months ago)

yes Im sorry, here is the photo




it says +100 degrees, is that enough?

thank you man, I was confused with pour and no pour agar


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Re: Agar as easy as 1-2-3! [Re: Samalander]
    #26257981 - 10/17/19 08:56 AM (4 years, 4 months ago)

I guess I will turn the stove to lowest temperature and stick some coins on a PC valve for higher psi and hope for the best


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Re: Agar as easy as 1-2-3! [Re: Samalander]
    #26257995 - 10/17/19 09:02 AM (4 years, 4 months ago)

Is the lid stamped as well? Sometimes ppl get screwed like that where the dish is PP5 and the lid isn't and melts in the PC.

Wouldn't be the end of the world, just a little wasted effort and small mess

Why the higher psi? 15 psi for 25 min should be sufficient.

Just be sure to allow it to vent for 10 mins before adding your weight and building pressure


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Re: Agar as easy as 1-2-3! [Re: Samalander]
    #26257998 - 10/17/19 09:04 AM (4 years, 4 months ago)

haha YES! it has pp five and a triangle! thank you man! didnt see that last sentance with a triangle
much love !


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Re: Agar as easy as 1-2-3! [Re: Samalander] * 1
    #26258003 - 10/17/19 09:06 AM (4 years, 4 months ago)

I love giving the 1 or 2 answers i actually do have lol, anytime man :chugbeer:


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Re: Agar as easy as 1-2-3! [Re: Camera93]
    #26259907 - 10/18/19 12:02 AM (4 years, 4 months ago)

one cup is 240 ml. right?
shiiit, Im all over this page...sorry  :facepalm3:


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Re: Agar as easy as 1-2-3! [Re: Samalander]
    #26259919 - 10/18/19 12:17 AM (4 years, 4 months ago)

Quote:

Samalander said:
one cup is 240 ml. right?
shiiit, Im all over this page...sorry  :facepalm3:




1 cup = 240ml
1/2 tsp = 2.5ml


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Re: Agar as easy as 1-2-3! [Re: murderlabz]
    #26259940 - 10/18/19 12:49 AM (4 years, 4 months ago)

:thumbup::thumbup::thumbup:


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #26794868 - 06/30/20 03:46 PM (3 years, 7 months ago)

Thanks for the details. First attempt underway, and after having to scrap 7 of 10 jars noc'd with a MS syringe I'm going to get proper and go agar to grain for the next trial.


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Re: Agar as easy as 1-2-3! [Re: Sacral_Z]
    #26803827 - 07/04/20 03:34 PM (3 years, 7 months ago)

Forum vpp


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #27685125 - 03/06/22 01:50 PM (1 year, 11 months ago)

Sorry - confused here.  Are the amounts for the recipe ingredients correct in this text or in the video?  They are different.  Thanks!


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Re: Agar as easy as 1-2-3! [Re: BuddyB]
    #27685344 - 03/06/22 05:07 PM (1 year, 11 months ago)

Quote:

BuddyB said:
Sorry - confused here.  Are the amounts for the recipe ingredients correct in this text or in the video?  They are different.  Thanks!



use either both work


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #27703837 - 03/21/22 05:39 PM (1 year, 10 months ago)

awesome munch :heart::mushroom2:


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #27759399 - 05/01/22 11:09 PM (1 year, 9 months ago)

I noticed my potato flakes and agar started to separate. Any idea why?


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Re: Agar as easy as 1-2-3! [Re: svgar_cvnt]
    #27759462 - 05/02/22 12:40 AM (1 year, 9 months ago)

You must prep the potato flakes before hand.


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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #27850294 - 07/05/22 11:18 AM (1 year, 7 months ago)

This recipe has recently been amended.  This is the new section:

LME: (optional)
- 2 grams light malt extract
- 2 grams agar
- 1/2 cup water
- optional: food coloring


Has anyone tried this technique yet and willing to share experiences with it versus the original recipe of?:

- 1 gram honey or raw sugar
- 2 grams agar
- 3 grams potato flakes (plain instant mashed potatos)
- 1/2 cup water
- optional: food coloring


Thanks!


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OfflineMushroplex
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I'm a teapot


Registered: 10/30/22
Posts: 35
Last seen: 9 months, 25 days
Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #28034360 - 11/05/22 12:27 PM (1 year, 3 months ago)

Gonna do this now thanks:thumbup:


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OfflineMushroplex
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I'm a teapot


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Posts: 35
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #28034381 - 11/05/22 12:33 PM (1 year, 3 months ago)

I was saying the same thing to myself


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OfflineScrewup
Googles your dumb questions
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Re: Agar as easy as 1-2-3! [Re: Mushroplex] * 1
    #28034396 - 11/05/22 12:39 PM (1 year, 3 months ago)

You talkin to yourself there brutha


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OfflineMushroplex
Mycology Enthusiast
I'm a teapot


Registered: 10/30/22
Posts: 35
Last seen: 9 months, 25 days
Re: Agar as easy as 1-2-3! [Re: Munchauzen]
    #28046001 - 11/11/22 07:51 PM (1 year, 3 months ago)

:eek: Great Tech on this video apply the knowledge and made some great agar so far I transferred into this agar plate that I made using your instructions and so far three out of the four went great one of them did have contamination but I do know that's part of the game thanks again munch


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OfflineMushroplex
Mycology Enthusiast
I'm a teapot


Registered: 10/30/22
Posts: 35
Last seen: 9 months, 25 days
Re: Agar as easy as 1-2-3! [Re: a-a-ron]
    #28046004 - 11/11/22 07:53 PM (1 year, 3 months ago)

I tried this recipe the only thing that I did is I added gypsum so far so good four out of the three that I experimented on one of them came out contaminated the other three are going full throttle how was your experience?


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Offlinemacsone41
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Registered: 07/07/23
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Last seen: 2 hours, 41 minutes
Re: Agar as easy as 123! [Re: Munchauzen]
    #28396572 - 07/15/23 02:16 PM (7 months, 5 days ago)

I'm trying this recipe thanks


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