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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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FalseMaria
Perverted Goddess of Metropolis



Registered: 08/06/07
Posts: 767
Loc: Canada
Last seen: 7 years, 9 months
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Sterile Technique TEK Available?
#23184075 - 05/03/16 12:07 PM (8 years, 8 months ago) |
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I am by no means a beginner, and know the bulk of what needs to be done to have a sterile technique. That said, I've been gone a while, and I am a forgetful one.
I've tried the search feature, but I can't seem to find any write-up that describes sterile technique in detail. Is there such a document/thread that might exist?
-------------------- ✲゚。.(✿╹◡╹)ノ☆.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
#23184080 - 05/03/16 12:09 PM (8 years, 8 months ago) |
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dont stir up air in the SAb while moving your arms and never move your hands over open jars of sterile media.
what are you looking to do?
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FalseMaria
Perverted Goddess of Metropolis



Registered: 08/06/07
Posts: 767
Loc: Canada
Last seen: 7 years, 9 months
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Re: Sterile Technique TEK Available? [Re: spacechildo]
#23184104 - 05/03/16 12:16 PM (8 years, 8 months ago) |
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I have a flow hood, so that does not apply. I dunno, I remember reading a list of things to do when you do sensitive stuff.
- shower
- wear clean clothes
- Lysol bomb
- flame sterilise needle
And on and on. Obviously diff steps would apply to different phases of your work, but I thought there might be a compiled list of best practices.
-------------------- ✲゚。.(✿╹◡╹)ノ☆.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
#23184120 - 05/03/16 12:20 PM (8 years, 8 months ago) |
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Basically all those points can be skipped, save for the last one. When using a hood make sure nothing comes between the filter and your work.
Sterile tek is more common sense than anything even though it is harder than one assumes it is when starting out. Just think before you move, assess your movements and you will be fine.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
#23184128 - 05/03/16 12:21 PM (8 years, 8 months ago) |
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But what are you going to grow? cakes, monotub, trays, bottles...
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 11 months, 20 days
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
#23184139 - 05/03/16 12:23 PM (8 years, 8 months ago) |
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Sterile technique is just that... a technique... not really a TEK.
Anyways, it sounds like you still remember the basics... 
Good luck... nice to hear you are back in the hobby!
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin
Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
#23184141 - 05/03/16 12:23 PM (8 years, 8 months ago) |
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well then dont move your hands between the FH filter and your work. ISO'ed up gloves are good as training wheels, showering, clean clothes and all that is moot.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Sterile Technique TEK Available? [Re: Josex]
#23184142 - 05/03/16 12:23 PM (8 years, 8 months ago) |
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Sterile technique is the same whatever you inoculate, for the most part.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Sterile Technique TEK Available? [Re: Supalemonhaze]
#23184148 - 05/03/16 12:25 PM (8 years, 8 months ago) |
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Quote:
Supalemonhaze said: Sterile technique is the same whatever you inoculate, for the most part.
Yes, but if he wants to do agar I'm thinking of a link he can use.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Sterile Technique TEK Available? [Re: Josex]
#23184175 - 05/03/16 12:28 PM (8 years, 8 months ago) |
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Hell, shoot it. Plates, jars, what's the difference?
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FalseMaria
Perverted Goddess of Metropolis



Registered: 08/06/07
Posts: 767
Loc: Canada
Last seen: 7 years, 9 months
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Re: Sterile Technique TEK Available? [Re: Supalemonhaze]
#23184492 - 05/03/16 01:35 PM (8 years, 8 months ago) |
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I am about to do Agar in front of a flow hood. And yeah, TEK is not the right term, I get that.
-------------------- ✲゚。.(✿╹◡╹)ノ☆.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Sterile Technique TEK Available? *DELETED* [Re: FalseMaria]
#23184503 - 05/03/16 01:38 PM (8 years, 8 months ago) |
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Post deleted by JosexReason for deletion: ,
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: Sterile Technique TEK Available? [Re: Josex]
#23184542 - 05/03/16 01:45 PM (8 years, 8 months ago) |
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stolen vids dude..
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Sterile Technique TEK Available? [Re: spacechildo]
#23184551 - 05/03/16 01:46 PM (8 years, 8 months ago) |
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Ups, yeah. deleted already.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Sterile Technique TEK Available? [Re: spacechildo]
#23184556 - 05/03/16 01:47 PM (8 years, 8 months ago) |
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No shit, I wonder why RR doesn't shut those down.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: Sterile Technique TEK Available? [Re: Supalemonhaze]
#23184602 - 05/03/16 01:56 PM (8 years, 8 months ago) |
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RR, the king of internet..
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FalseMaria
Perverted Goddess of Metropolis



Registered: 08/06/07
Posts: 767
Loc: Canada
Last seen: 7 years, 9 months
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Re: Sterile Technique TEK Available? [Re: spacechildo]
#23185172 - 05/03/16 04:19 PM (8 years, 8 months ago) |
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I have the RR vids at home (legally)
-------------------- ✲゚。.(✿╹◡╹)ノ☆.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
#23185183 - 05/03/16 04:21 PM (8 years, 8 months ago) |
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he does agar in front of FH in one of those..
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
#23185226 - 05/03/16 04:34 PM (8 years, 8 months ago) |
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OP your posts don't jibe man. Why did you even make a thread to ask?
Quote:
FalseMaria said: I am by no means a beginner, and know the bulk of what needs to be done to have a sterile technique. That said, I've been gone a while, and I am a forgetful one.
I've tried the search feature, but I can't seem to find any write-up that describes sterile technique in detail. Is there such a document/thread that might exist?
Quote:
FalseMaria said: I have a flow hood, so that does not apply. I dunno, I remember reading a list of things to do when you do sensitive stuff.
- shower
- wear clean clothes
- Lysol bomb
- flame sterilise needle
And on and on. Obviously diff steps would apply to different phases of your work, but I thought there might be a compiled list of best practices.
Quote:
FalseMaria said: I am about to do Agar in front of a flow hood. And yeah, TEK is not the right term, I get that.
Quote:
FalseMaria said: I have the RR vids at home (legally)
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FalseMaria
Perverted Goddess of Metropolis



Registered: 08/06/07
Posts: 767
Loc: Canada
Last seen: 7 years, 9 months
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Re: Sterile Technique TEK Available? [Re: Josex]
#23185379 - 05/03/16 05:10 PM (8 years, 8 months ago) |
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Well, because there's tons of teks and methods out there not covered in RRs vids. I was just looking for something that was exhaustive and complete for each type of mycology activity.
I just mentioned agar was my next step because someone asked. I also plan to do LI, MS, G2G, etc.
It was more curiosity. Shit, if it's common sense plus all the shit I already do, then my question has been answered.
-------------------- ✲゚。.(✿╹◡╹)ノ☆.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
#23185392 - 05/03/16 05:14 PM (8 years, 8 months ago) |
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Watch the RR vids again. I still watch them every once in a while and often pick up something I didn't catch before or have forgotten. They are an excellent refresher.
-------------------- The Basics
A little civility goes a long way
The Noob Forum
The Hammock Hangers' Forum
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: Sterile Technique TEK Available? [Re: SpitballJedi]
#23185394 - 05/03/16 05:15 PM (8 years, 8 months ago) |
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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FalseMaria
Perverted Goddess of Metropolis



Registered: 08/06/07
Posts: 767
Loc: Canada
Last seen: 7 years, 9 months
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Re: Sterile Technique TEK Available? [Re: PussyFart]
#23185407 - 05/03/16 05:18 PM (8 years, 8 months ago) |
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Yup, doing exactly that with his visa, and this web site.
-------------------- ✲゚。.(✿╹◡╹)ノ☆.
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