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OfflineFalseMaria
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Sterile Technique TEK Available?
    #23184075 - 05/03/16 12:07 PM (8 years, 8 months ago)

I am by no means a beginner, and know the bulk of what needs to be done to have a sterile technique. That said, I've been gone a while, and I am a forgetful one.

I've tried the search feature, but I can't seem to find any write-up that describes sterile technique in detail. Is there such a document/thread that might exist?


--------------------
✲゚。.(✿╹◡╹)ノ☆.

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Offlinespacechildo
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
    #23184080 - 05/03/16 12:09 PM (8 years, 8 months ago)

dont stir up air in the SAb while moving your arms
and never move your hands over open jars of sterile media.

what are you looking to do?

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OfflineFalseMaria
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Re: Sterile Technique TEK Available? [Re: spacechildo]
    #23184104 - 05/03/16 12:16 PM (8 years, 8 months ago)

I have a flow hood, so that does not apply. I dunno, I remember reading a list of things to do when you do sensitive stuff.

  • shower
  • wear clean clothes
  • Lysol bomb
  • flame sterilise needle


And on and on. Obviously diff steps would apply to different phases of your work, but I thought there might be a compiled list of best practices.


--------------------
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InvisibleSupalemonhaze
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
    #23184120 - 05/03/16 12:20 PM (8 years, 8 months ago)

Basically all those points can be skipped, save for the last one. When using a hood make sure nothing comes between the filter and your work.

Sterile tek is more common sense than anything even though it is harder than one assumes it is when starting out. Just think before you move, assess your movements and you will be fine.

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InvisibleJosex
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
    #23184128 - 05/03/16 12:21 PM (8 years, 8 months ago)

But what are you going to grow? cakes, monotub, trays, bottles...

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OfflineFreeWorldOrder
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
    #23184139 - 05/03/16 12:23 PM (8 years, 8 months ago)

Sterile technique is just that... a technique... not really a TEK.

Anyways, it sounds like you still remember the basics... :thumbup:

Good luck... nice to hear you are back in the hobby!


--------------------
"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

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PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK

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Offlinespacechildo
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
    #23184141 - 05/03/16 12:23 PM (8 years, 8 months ago)

well then dont move your hands between the FH filter and your work.
ISO'ed up gloves are good as training wheels, showering, clean clothes and all that is moot.

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InvisibleSupalemonhaze
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Re: Sterile Technique TEK Available? [Re: Josex]
    #23184142 - 05/03/16 12:23 PM (8 years, 8 months ago)

Sterile technique is the same whatever you inoculate, for the most part.

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InvisibleJosex
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Re: Sterile Technique TEK Available? [Re: Supalemonhaze]
    #23184148 - 05/03/16 12:25 PM (8 years, 8 months ago)

Quote:

Supalemonhaze said:
Sterile technique is the same whatever you inoculate, for the most part.




Yes, but if he wants to do agar I'm thinking of a link he can use.

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InvisibleSupalemonhaze
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Re: Sterile Technique TEK Available? [Re: Josex]
    #23184175 - 05/03/16 12:28 PM (8 years, 8 months ago)

Hell, shoot it. Plates, jars, what's the difference?

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OfflineFalseMaria
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Re: Sterile Technique TEK Available? [Re: Supalemonhaze]
    #23184492 - 05/03/16 01:35 PM (8 years, 8 months ago)

I am about to do Agar in front of a flow hood. And yeah, TEK is not the right term, I get that.


--------------------
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InvisibleJosex
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Re: Sterile Technique TEK Available? *DELETED* [Re: FalseMaria]
    #23184503 - 05/03/16 01:38 PM (8 years, 8 months ago)

Post deleted by Josex

Reason for deletion: ,


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Offlinespacechildo
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Re: Sterile Technique TEK Available? [Re: Josex]
    #23184542 - 05/03/16 01:45 PM (8 years, 8 months ago)

stolen vids dude.. :rolleyes:

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InvisibleJosex
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Re: Sterile Technique TEK Available? [Re: spacechildo]
    #23184551 - 05/03/16 01:46 PM (8 years, 8 months ago)

Ups, yeah. deleted already.

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InvisibleSupalemonhaze
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Re: Sterile Technique TEK Available? [Re: spacechildo]
    #23184556 - 05/03/16 01:47 PM (8 years, 8 months ago)

No shit, I wonder why RR doesn't shut those down.

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Offlinespacechildo
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Re: Sterile Technique TEK Available? [Re: Supalemonhaze]
    #23184602 - 05/03/16 01:56 PM (8 years, 8 months ago)

RR, the king of internet..

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OfflineFalseMaria
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Re: Sterile Technique TEK Available? [Re: spacechildo]
    #23185172 - 05/03/16 04:19 PM (8 years, 8 months ago)

I have the RR vids at home (legally)


--------------------
✲゚。.(✿╹◡╹)ノ☆.

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Offlinespacechildo
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
    #23185183 - 05/03/16 04:21 PM (8 years, 8 months ago)

he does agar in front of FH in one of those..

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InvisibleJosex
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
    #23185226 - 05/03/16 04:34 PM (8 years, 8 months ago)

OP your posts don't jibe man. Why did you even make a thread to ask?

Quote:

FalseMaria said:
I am by no means a beginner, and know the bulk of what needs to be done to have a sterile technique. That said, I've been gone a while, and I am a forgetful one.

I've tried the search feature, but I can't seem to find any write-up that describes sterile technique in detail. Is there such a document/thread that might exist?



Quote:

FalseMaria said:
I have a flow hood, so that does not apply. I dunno, I remember reading a list of things to do when you do sensitive stuff.

  • shower
  • wear clean clothes
  • Lysol bomb
  • flame sterilise needle


And on and on. Obviously diff steps would apply to different phases of your work, but I thought there might be a compiled list of best practices.



Quote:

FalseMaria said:
I am about to do Agar in front of a flow hood. And yeah, TEK is not the right term, I get that.




Quote:

FalseMaria said:
I have the RR vids at home (legally)



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OfflineFalseMaria
Perverted Goddess of Metropolis
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Registered: 08/06/07
Posts: 767
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Last seen: 7 years, 9 months
Re: Sterile Technique TEK Available? [Re: Josex]
    #23185379 - 05/03/16 05:10 PM (8 years, 8 months ago)

Well, because there's tons of teks and methods out there not covered in RRs vids. I was just looking for something that was exhaustive and complete for each type of mycology activity.

I just mentioned agar was my next step because someone asked. I also plan to do LI, MS, G2G, etc.

It was more curiosity. Shit, if it's common sense plus all the shit I already do, then my question has been answered.


--------------------
✲゚。.(✿╹◡╹)ノ☆.

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InvisibleSpitballJedi
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Re: Sterile Technique TEK Available? [Re: FalseMaria]
    #23185392 - 05/03/16 05:14 PM (8 years, 8 months ago)

Watch the RR vids again. I still watch them every once in a while and often pick up something I didn't catch before or have forgotten. They are an excellent refresher.


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A little civility goes a long way

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OfflinePussyFart
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Re: Sterile Technique TEK Available? [Re: SpitballJedi]
    #23185394 - 05/03/16 05:15 PM (8 years, 8 months ago)

:fuckinawesome:

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OfflineFalseMaria
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Registered: 08/06/07
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Re: Sterile Technique TEK Available? [Re: PussyFart]
    #23185407 - 05/03/16 05:18 PM (8 years, 8 months ago)

Yup, doing exactly that with his visa, and this web site. :smile:


--------------------
✲゚。.(✿╹◡╹)ノ☆.

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