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OfflineDrMushroom
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Best way to inoculate PF jars with agar
    #23182651 - 05/03/16 01:13 AM (8 years, 8 months ago)

Whats the best way to do this?

I would imagine it ultimately comes down to getting the mycelium suspended in a solution of sorts so my question is more of how i make that mycelium suspension. I want to continue doing PF jars for the time being, at very least once with all my different spores ive collected instead of going straight into bulk, im happy with my yeilds anyway, so ill stick with whats comfortable, easier (and faster i think too).

Another thing though i want to try is cloning, albeit still on pf tek, so, as it is, i need to be able to transfer mycelium on agar via a syringe into the jar.


Can i just drop some mycelium in a liquid culture and shake it every now and then? should i make mycelium water squirting and sucking water off the stuff on agar, and do i use that directly or would it be better left to grow in the liquid culture medium? does that potentially harm/compromise the mycelium on the agar doing that?
what are my options?

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OfflineNDStepp84
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Re: Best way to inoculate PF jars with agar [Re: DrMushroom]
    #23182726 - 05/03/16 02:04 AM (8 years, 8 months ago)

Using pasty plates and a sterilized syringe full of water squirt onto the surface of the myc use the tip of the needle to scratch all the myc loose and draw it back up into the syringe then you have a syringe of LI you can use just like you would a spore syringe to inoculate your cakes:super:


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:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread

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InvisibleSupalemonhaze
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Re: Best way to inoculate PF jars with agar [Re: NDStepp84]
    #23182811 - 05/03/16 03:28 AM (8 years, 8 months ago)

You can also make LCs with wedges and inject like you would with spores.

Wedges straight to cake might work if you use proper filters and skip the verm barrier, it will be slower than an LI or LC though.

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OfflineSloppyJoseph
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Re: Best way to inoculate PF jars with agar [Re: NDStepp84]
    #23182818 - 05/03/16 03:36 AM (8 years, 8 months ago)

Quote:

NDStepp84 said:
Using pasty plates and a sterilized syringe full of water squirt onto the surface of the myc use the tip of the needle to scratch all the myc loose and draw it back up into the syringe then you have a syringe of LI you can use just like you would a spore syringe to inoculate your cakes:super:




This way is ok, but locn9ne just had some serious success using Munches blenderless LI on cakes.

Basically
-PC about 100cc of water In an unmodified pint jar. (Do this with your plates)
-in your SAB drop in your colonized agar using proper sterile technique.
-take the jar with the agar and water outside away from the SAB and shake the shit out of it until the agar is all broken up and the mycelium is distributed throughout the solution
-bring back into SAB and use proper sterile technique to suck up that solution into a sterile syringe

Gets a lot more mycelium per cc that just squirting and scraping. Munch has a video on how he does this too. Just search blenderless li


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InvisibleSupalemonhaze
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Re: Best way to inoculate PF jars with agar [Re: SloppyJoseph]
    #23182823 - 05/03/16 03:41 AM (8 years, 8 months ago)

I tried that yesterday, didn't really like it though after like 4 jars in. Dropped wedges in the rest of the jars but I guess I should see the results before I hand down the ultimatum. I think an LC is a hundred times better than blenderless LI. Now the slurry, that is something I really want to try. Too bad those blenders aren't sold in europe.

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OfflineSloppyJoseph
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Re: Best way to inoculate PF jars with agar [Re: Supalemonhaze]
    #23182827 - 05/03/16 03:48 AM (8 years, 8 months ago)

Yeah you can't find a damn thing over ther, can you? :lol:

LC and blenderless LI have their advantages. For cakes I think it's clear though, LC is kind of a waste of time cause for such a small container you really only need a certain degree of mycelium in your solution to really be successful. But for grains it'll depend on what you want. If you want to make an LC for repeated use and storage that's nice, but using 5 cc of LC and 25cc of blenderless LI will get you results pretty much the same. Blenderless is just a kind of one and done method.


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InvisibleSupalemonhaze
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Re: Best way to inoculate PF jars with agar [Re: SloppyJoseph]
    #23182840 - 05/03/16 03:58 AM (8 years, 8 months ago)

I wonder why you guys use blenderless over there, those blenders are pretty darn cheap and easy to get and the results surely blow blenderless LI out of the water.

I honestly don't like LCs much nowadays, too risky and once you do a few grows, things aren't as urgent as they were when you start so wedges do fine once you have the patience for them. I would use an LC if I wanted a bunch of jars inoculated and won't have the time to expand all that mycelium on agar or if for some reason I needed a jar to colonize ridiculously fast but that doesn't happen much.

Blended LI would be a good addition for any grower though, it's about as safe as using wedges yet as fast as LC, if not faster. I was thinking maybe once I have some extra cash I should buy the oster blade and washer and see if I can fabricate something to make it work on an other appliance.

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OfflineSloppyJoseph
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Re: Best way to inoculate PF jars with agar [Re: Supalemonhaze]
    #23182850 - 05/03/16 04:04 AM (8 years, 8 months ago)

Honestly it's just a little bit of laziness. Blenderless is so accessible. If I ever stumble on an oster in the store I'll buy it right there, but I've spent so much time running around buying necessary shit for this hobby that the unnecessary but helpful shit (like gypsum, silicon, sfd, etc) are not my top priority. Slurry is definitely the way to go though, 100%.

But if you are going to use a wedge, all I'm saying is blenderless is superior. That one wedge can inoculate 6 jars faster and just as clean as if you dropped that same plate into 1 jar.


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OfflineGreenRabbit
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Re: Best way to inoculate PF jars with agar [Re: SloppyJoseph]
    #23182873 - 05/03/16 04:23 AM (8 years, 8 months ago)

Quote:

SloppyJoseph said:
Honestly it's just a little bit of laziness. Blenderless is so accessible.




I've seen all the teks on how to make blender lids/jars etc...
It all seems like such a hassle.
I mean, all I have to do is shake the fuck out of this jar, and it'll work.
Cleaning that blade after would be annoying too.

I'm not trying to grow as much as possible.
I just want as much as possible, with as little work as possible.

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InvisibleSupalemonhaze
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Registered: 10/02/15
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Loc: 12" down Europe's butthole
Re: Best way to inoculate PF jars with agar [Re: SloppyJoseph]
    #23182874 - 05/03/16 04:23 AM (8 years, 8 months ago)

For speed of colonization you're probably right yet I hated it for some reason. Wedges seemed better once I started pouring it.

I don't use whole plates though when using wedges, I find there isn't much difference in colonization between a whole plate and a quarter or a half plate. When I want faster colonization with wedges I like to use multiple smaller ones, that is much better than a big ass wedge. It also seems that once you start making batches of grains with wedges in weekly increments, speed becomes a trivial issue as you will always have something to spawn.

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InvisiblePastywhyteMDiscord
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Re: Best way to inoculate PF jars with agar [Re: Supalemonhaze]
    #23183092 - 05/03/16 06:56 AM (8 years, 8 months ago)

Personally I feel that doing agar for the sole purpose of inoculating a pf jar to be a step back. I love agar, it kicks ass. But if you are going to be using it, you might as well bite the bullet and do grains as well.

Same thing with liquid. If you are going to the trouble to make LC or LI then putting those to cakes seems counter intuitive. Yes you can do it. But it's like when guys put a $5000 stereo in a car worth $500. Get them shits on grains.

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InvisibleSupalemonhaze
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Re: Best way to inoculate PF jars with agar [Re: Pastywhyte]
    #23183111 - 05/03/16 07:01 AM (8 years, 8 months ago)

That's true. Cakes suck whatever your choice of inoculant is IMO.

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InvisibleLocN9ne
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Re: Best way to inoculate PF jars with agar [Re: Supalemonhaze] * 1
    #23183161 - 05/03/16 07:20 AM (8 years, 8 months ago)

I like doing cakes... :singletear:

Polyfil stuffed lids, no verm barrier, blenderless LI would be the way to go if you want to use agar for cakes... I used LC a few times and it's cool and all, but with something as small as brf cakes LI is plenty good... They take longer to consolidate than they do to colonize.


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Edited by LocN9ne (05/03/16 07:24 AM)

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InvisibleJosex
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Re: Best way to inoculate PF jars with agar [Re: GreenRabbit]
    #23183287 - 05/03/16 08:17 AM (8 years, 8 months ago)

Quote:

GreenRabbit said:
Quote:

SloppyJoseph said:
Honestly it's just a little bit of laziness. Blenderless is so accessible.




I've seen all the teks on how to make blender lids/jars etc...
It all seems like such a hassle.
I mean, all I have to do is shake the fuck out of this jar, and it'll work.
Cleaning that blade after would be annoying too.

I'm not trying to grow as much as possible.
I just want as much as possible, with as little work as possible.




I use a pp5 container and a ball of tangled up wire.


That thing can destroy anything, even hard brf plates on the dry side. But you only need to do that if you live in the third world, otherwise you can buy this baby...

Of course, you'd still need a proper blender jar to do PF cake and grain slurries.

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