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Offline00Burnout
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New cultivator
    #23182086 - 05/02/16 09:44 PM (8 years, 8 months ago)

So I'll be growing mushrooms for the first time and have a few questions. I've already picked out my strain, substrate and casing material. I decided for simplicity and lack of necessary equipment and facilities that I'll be spawning in 2lb rye grain mycobags and fruiting in a monotub. My question is should I spread out my colonized substrate or keep it as a cake? Does casing increase the yield or potency or just serve as a protective layer, and if it's best to leave my sub in a cake-like structure do I still case it or would that much casing restrict air exchange to the point that a HEPA pump would be necessary?

I feel like I'm making things a little more complicated than they need to be, granted my ideal work environment for practicing sterile technique would be a class 1 clean room.


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OfflinePussyFart
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Re: New cultivator [Re: 00Burnout]
    #23182135 - 05/02/16 10:02 PM (8 years, 8 months ago)

Quote:

00Burnout said:
So I'll be growing mushrooms for the first time and have a few questions. I've already picked out my strain, substrate and casing material. I decided for simplicity and lack of necessary equipment and facilities that I'll be spawning in 2lb rye grain mycobags and fruiting in a monotub. My question is should I spread out my colonized substrate or keep it as a cake?



Spread it out, like it says to do in every single monotub tek there is.

Quote:

00Burnout said:
Does casing increase the yield or potency or just serve as a protective layer



A casing layer is a layer of non (or very low) nutritious material that is spread over a colonized substrate to help aid in pinning and moisture retention.

It is not going to increase yield or potency, and is not really necessary in a monotub.

Quote:

00Burnout said:
and if it's best to leave my sub in a cake-like structure do I still case it or would that much casing restrict air exchange to the point that a HEPA pump would be necessary?



If you use a casing layer, it will become part of the substrate, meaning it will not restrict any FAE.

No pumps or any electronic garbage is ever needed in a monotub setup.

Quote:

00Burnout said:
I feel like I'm making things a little more complicated than they need to be, granted my ideal work environment for practicing sterile technique would be a class 1 clean room.



A simple SAB(Still Air box) is all you need for sterile work.

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Invisibledankington
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Re: New cultivator [Re: 00Burnout]
    #23182145 - 05/02/16 10:05 PM (8 years, 8 months ago)

You would mix those grains with a pasteurized sub when they're fully colonized inside your tub. If you're doing 2lb. bags make sure you have a larger sized PC.

No one knows for sure if you would benefit from a casing layer, there are too many variables. I think you have a few techniques mixed.

A regular polyfill stuffed mono-tub would be fine, no need for the HEPA.

You should really start with agar first so you can make an LI for your grains if you're going into bulk. Even if not this round, you should start looking into it now.

Just use a tiny bit of spore solution, being I know you're not going to listen, and are going to put the spores to grains directly anyways.

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OfflineGlowsic
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Re: New cultivator [Re: dankington]
    #23182160 - 05/02/16 10:08 PM (8 years, 8 months ago)

I made a puppet account and got banned.

Edited by PussyFart (05/02/16 10:12 PM)

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Offline00Burnout
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Re: New cultivator [Re: PussyFart]
    #23182208 - 05/02/16 10:21 PM (8 years, 8 months ago)

Thanks for the tips. As for the clean room comment was more a joke on my ocd, hence all the questions that I'm sure are asked a thousand times on this site.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offline00Burnout
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Re: New cultivator [Re: dankington]
    #23182250 - 05/02/16 10:33 PM (8 years, 8 months ago)

Actually I've already looked into making cultures, but rather than starting a culture right away I'm going to collect spore prints to make cultures when I have a better work area. I've done a little research on cloning as well which seems worth experimenting with once I have my setup finished


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offline00Burnout
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Re: New cultivator [Re: 00Burnout]
    #23187971 - 05/04/16 08:58 AM (8 years, 8 months ago)

One more question. How many mL should I inoculate my bags with? I've read a few posts with the same question but never found a clear answer. Again I'm using myco bags with 2lbs presterilized rye grain, would 2-3 mL suffice without having an unnecessary wait time for full colonization?


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No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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OfflinePussyFart
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Re: New cultivator [Re: 00Burnout]
    #23187978 - 05/04/16 09:02 AM (8 years, 8 months ago)

I wouldn't use any more than 1 ML personally.

At about 30% colonization you can mix up the grains to spread out the inoculation points which will speed up the process.

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Offline00Burnout
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Re: New cultivator [Re: PussyFart]
    #23188952 - 05/04/16 03:04 PM (8 years, 8 months ago)

Thanks, any other tips? I'd rather read the same advice a million times than over look some vital information and screw it all up. This seems like a fairly easy hobby so long as sterile technique is practiced, and I've even read some posts where people have improperly sterilized their sub and still got contam free batches.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offline00Burnout
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Re: New cultivator [Re: 00Burnout]
    #23189541 - 05/04/16 06:00 PM (8 years, 8 months ago)

Ok so I think I have this sorted out. inoculate my bags, when they're fully colonized consolidate for 2-3 days then mix with my bulk sub in my FR and let nature take its course. I'm sure I'll be scourned by nearly every cultivator for this but I'm using sterilized bulk sub instead of pasteurized (I'm a clean freak I scrub my bedroom with bleach once a week minimum so I'm not to worried about contams) all my supplies are coming from mycohaus so going with their bulk sub is a little more practical for me for now even though its going to cost a little more. I might be biting off more than I can chew for a newbie (12lbs of grain and 10lbs bulk sub) but I'm fairly confident that all will go well. One other little detail I've left out, I'm going with GTs even though I've heard that B  is the best "strain" for noobs but a cube is a cube in my book (PE being the exception from my understanding). Hopefully this is the last of my redundant questions, of course when I get everything started I'll start a new post following my cultivation.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Invisibledankington
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Re: New cultivator [Re: 00Burnout]
    #23189616 - 05/04/16 06:28 PM (8 years, 8 months ago)

If you already know sterilizing is a bad, why do it? Your room isn't the only concern in this case.

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Offline00Burnout
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Re: New cultivator [Re: dankington]
    #23189651 - 05/04/16 06:37 PM (8 years, 8 months ago)

Because it's more practical for me to get all my supplies in one order on one site and mycohaus doesn't offer anything but sterilized bulk sub. I've read mixed opinions on sterilized vs pasteurized bulk sub and quite a few people have gotten good results from sterilized.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offlinemushpunx
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Re: New cultivator [Re: 00Burnout]
    #23189692 - 05/04/16 06:49 PM (8 years, 8 months ago)

Quote:

00Burnout said:
Because it's more practical for me to get all my supplies in one order on one site and mycohaus doesn't offer anything but sterilized bulk sub. I've read mixed opinions on sterilized vs pasteurized bulk sub and quite a few people have gotten good results from sterilized.





It depends on the bulk sub. Pretty much the ONLY bulk sub you can get away with sterilizing is coco coir.. and it doesnt really need to be treated at all. You can prep coir with cold water for a grow if you want.

Any bulk sub with straw, or manure in it... most everything besides coir need to be pasturized, not sterilized if you are going to spawn to a bulk substrate in open air or it will contaminate.





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Edited by mushpunx (05/04/16 06:51 PM)

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InvisibleSpitballJedi
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Re: New cultivator [Re: mushpunx]
    #23189767 - 05/04/16 07:08 PM (8 years, 8 months ago)

It also depends on spawning method. If your sub is sterilized manure or straw, (I'm not going down the list) then it will need to remain in sterile conditions until 100% colonized. That means you will need to put your spawn in there while in a SAB or in front of a flow hood.

If it's just CVG, then you can spawn in open air. Pasteurized manure and straw can be spawned in open air, but not sterilized.


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Offlinemushpunx
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Re: New cultivator [Re: SpitballJedi]
    #23189780 - 05/04/16 07:12 PM (8 years, 8 months ago)

:whathesaid:


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Offline00Burnout
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Re: New cultivator [Re: mushpunx]
    #23189831 - 05/04/16 07:26 PM (8 years, 8 months ago)

Thanks. I'll look around and see if I can find coir at a local gardening store or maybe wal Mart if I'm lucky. I'm trying to keep my online purchases to a minimum, shipping is murderous. All together I'm trying to avoid pasteurizing buckets full of crap. Mycohaus carries bags with grain and all the goodies already sterilized but I can't find any solid info as to how efficient they are, and I have a feeling that I wouldn't get the results I'm after with them anyway. I wish I had the money to try all the teks but I've got to go as cheap and simple as I can till I can get the ball rolling.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Invisibledankington
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Re: New cultivator [Re: 00Burnout]
    #23189841 - 05/04/16 07:28 PM (8 years, 8 months ago)

You can find coir by the reptiles in a pet shop :thumbup:

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Offline00Burnout
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Re: New cultivator [Re: SpitballJedi]
    #23189856 - 05/04/16 07:32 PM (8 years, 8 months ago)

Quote:

SpitballJedi said:
It also depends on spawning method. If your sub is sterilized manure or straw, (I'm not going down the list) then it will need to remain in sterile conditions until 100% colonized. That means you will need to put your spawn in there while in a SAB or in front of a flow hood.

If it's just CVG, then you can spawn in open air. Pasteurized manure and straw can be spawned in open air, but not sterilized.




I'm using spawn bags with rye grain, and then innoculating my bulk sub, whatever it might, I'm still gathering info till Monday.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
Ghetto Greenhouse
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Offline00Burnout
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Re: New cultivator [Re: dankington]
    #23189878 - 05/04/16 07:36 PM (8 years, 8 months ago)

Quote:

dankington said:
You can find coir by the reptiles in a pet shop :thumbup:




Unfortunately the closest pet shop is a half hour away and my car isn't very reliable, if I wasn't afraid of the what might really be in it I'd just buy a bag of manure from wally world and a bail of straw but a little voice in my head is screaming "STAY AWAY FROM WALMART"


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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Offlinemushpunx
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Re: New cultivator [Re: 00Burnout]
    #23189885 - 05/04/16 07:37 PM (8 years, 8 months ago)

Quote:

00Burnout said:
Thanks. I'll look around and see if I can find coir at a local gardening store or maybe wal Mart if I'm lucky. I'm trying to keep my online purchases to a minimum, shipping is murderous. All together I'm trying to avoid pasteurizing buckets full of crap. Mycohaus carries bags with grain and all the goodies already sterilized but I can't find any solid info as to how efficient they are, and I have a feeling that I wouldn't get the results I'm after with them anyway. I wish I had the money to try all the teks but I've got to go as cheap and simple as I can till I can get the ball rolling.





Then you are better off learning to it yourself, this website is gunna rob you blind have you pay 50$ for 5$ worth of material


--------------------

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