|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
brazul117
Stranger
Registered: 01/08/16
Posts: 16
Last seen: 8 years, 7 months
|
BulkTEK Noob Help Plz!!!
#23181480 - 05/02/16 07:05 PM (8 years, 8 months ago) |
|
|
First of all thank you for reading my post!
I'm trying to move to BulkTEK and I have a few questions I hope I can get answered here. 1. 5 X canning jars of cooked rye seeds inoculated 8 days ago with a multispore syringe showing no signs of mycelial growth. is this normal? or has it stalled on all 5? (placed in same conditions that BRF cakes explode in, while colonizing that is.)
2. I have already had great success with BRF cakes and currently have quite a few in a second flush. my question is can you crumble up a colonized cake after picking off any aborts and use that to colonize a sterilized rye jar like you would grain to grain?
3. Finally, this may sound hella stupid but... if the rye jars i currently have stalled can i put some of tha crumbled cake into it and colonize or is it a complete loss?
Edited by brazul117 (05/02/16 07:08 PM)
|
Patsyblack
5% er
Registered: 05/01/16
Posts: 183
Last seen: 8 years, 7 months
|
Re: BulkTEK Noob Help Plz!!! [Re: brazul117]
#23181508 - 05/02/16 07:13 PM (8 years, 8 months ago) |
|
|
Quote:
brazul117 said: First of all thank you for reading my post!
I'm trying to move to BulkTEK and I have a few questions I hope I can get answered here. 1. 5 X canning jars of cooked rye seeds inoculated 8 days ago with a multispore syringe showing no signs of mycelial growth. is this normal? or has it stalled on all 5? (placed in same conditions that BRF cakes explode in, while colonizing that is.) Yes its normal for ms syringe 2. I have already had great success with BRF cakes and currently have quite a few in a second flush. my question is can you crumble up a colonized cake after picking off any aborts and use that to colonize a sterilized rye jar like you would grain to grain? No cake is dirty dirty spawn it will just contam
3. Finally, this may sound hella stupid but... if the rye jars i currently have stalled can i put some of tha crumbled cake into it and colonize or is it a complete loss?[quote no and no get some agar plates or inoculate more jars
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
|
Re: BulkTEK Noob Help Plz!!! [Re: Patsyblack]
#23182100 - 05/02/16 09:50 PM (8 years, 8 months ago) |
|
|
1. Not uncommon, spores can take a couple weeks to germinate.
2. No. Only sterile innoculant can be used to inoculate grains, under sterile conditions IE still air box or flow hood. During grain to grain transfer grains are poured directly from the master jar into receivers , under sterile conitions. A cake that Has been exposed to open air is covered in contaminants.
3. No. Total loss.
--------------------

Amateur Mycologists United
AMU Q&A
|
brazul117
Stranger
Registered: 01/08/16
Posts: 16
Last seen: 8 years, 7 months
|
Re: BulkTEK Noob Help Plz!!! [Re: mushpunx]
#23182138 - 05/02/16 10:02 PM (8 years, 8 months ago) |
|
|
Quote:
mushpunx said: 1. Not uncommon, spores can take a couple weeks to germinate.
2. No. Only sterile innoculant can be used to inoculate grains, under sterile conditions IE still air box or flow hood. During grain to grain transfer grains are poured directly from the master jar into receivers , under sterile conitions. A cake that Has been exposed to open air is covered in contaminants.
3. No. Total loss.
so basically leave them alone for a couple of weeks and if i see no changes then its DOA?
also, while im at it. what could cause a jar to fail if the Tek is followed correctly?
|
liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
|
Re: BulkTEK Noob Help Plz!!! [Re: mushpunx]
#23182140 - 05/02/16 10:03 PM (8 years, 8 months ago) |
|
|
If you must, you can use what grains have been colonized after washing away the uncolonized grains with clean water to spawn your bulk substrate.
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
|
Re: BulkTEK Noob Help Plz!!! [Re: brazul117]
#23182179 - 05/02/16 10:13 PM (8 years, 8 months ago) |
|
|

Dirty innoculant could cause a jar to contaminAte. Spore syringes are rarely sterile. Really for grains , spores should be g erminAted on agar and cleAn culture obtained to be used as innoculant
--------------------

Amateur Mycologists United
AMU Q&A
|
brazul117
Stranger
Registered: 01/08/16
Posts: 16
Last seen: 8 years, 7 months
|
Re: BulkTEK Noob Help Plz!!! [Re: mushpunx]
#23183357 - 05/03/16 08:50 AM (8 years, 8 months ago) |
|
|
so basically leave them alone for a couple of weeks and if i see no changes then its DOA?
also, while im at it. what could cause a jar to fail if the Tek is followed correctly?
|
dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
|
Re: BulkTEK Noob Help Plz!!! [Re: mushpunx]
#23183402 - 05/03/16 09:03 AM (8 years, 8 months ago) |
|
|
Quote:
mushpunx said:

That's a gorgeous plate, MP!
Brazul, he already answered that:
Quote:
mushpunx said:
Dirty innoculant could cause a jar to contaminAte. Spore syringes are rarely sterile...
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
|
Re: BulkTEK Noob Help Plz!!! [Re: brazul117]
#23183941 - 05/03/16 11:28 AM (8 years, 8 months ago) |
|
|
Quote:
. ]brazul117 said: so basically leave them alone for a couple of weeks and if i see no changes then its DOA?
also, while im at it. what could cause a jar to fail if the Tek is followed correctly?
Well what do you meam by fail? Fail to germinate or fail as in contaminate?
If you dont see germination by 3 weeks then Id start over.
--------------------

Amateur Mycologists United
AMU Q&A
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
|
Re: BulkTEK Noob Help Plz!!! [Re: brazul117]
#23183954 - 05/03/16 11:31 AM (8 years, 8 months ago) |
|
|
Hey thanks man! Its a PE isolate I haven't gotten around to testing yet
--------------------

Amateur Mycologists United
AMU Q&A
|
brazul117
Stranger
Registered: 01/08/16
Posts: 16
Last seen: 8 years, 7 months
|
Re: BulkTEK Noob Help Plz!!! [Re: mushpunx]
#23184620 - 05/03/16 02:01 PM (8 years, 8 months ago) |
|
|
Fail as in do nothing. No mycelium begins growing.
|
Wormi
Quiet Cultivator


Registered: 10/29/14
Posts: 278
Last seen: 5 years, 8 months
|
Re: BulkTEK Noob Help Plz!!! [Re: brazul117]
#23184692 - 05/03/16 02:20 PM (8 years, 8 months ago) |
|
|
Quote:
brazul117 said: First of all thank you for reading my post!
I'm trying to move to BulkTEK and I have a few questions I hope I can get answered here. 1. 5 X canning jars of cooked rye seeds inoculated 8 days ago with a multispore syringe showing no signs of mycelial growth. is this normal? or has it stalled on all 5? (placed in same conditions that BRF cakes explode in, while colonizing that is. LOL ok Yoda) 8 days with no growth could just mean weak spores. give it another week. If theres no growth start over. Make sure you are hydrating correctly and your spore source is reputable.
2. I have already had great success with BRF cakes and currently have quite a few in a second flush. my question is can you crumble up a colonized cake after picking off any aborts and use that to colonize a sterilized rye jar like you would grain to grain? Technically yes. Realistically no. Too many contams.
3. Finally, this may sound hella stupid but... if the rye jars i currently have stalled can i put some of tha crumbled cake into it and colonize or is it a complete loss? Not unless you do it in a sterilized environment. If they have stalled you either have bad spores or you hydrated the grains incorrectly. At this point in time your success is very low trying to do this.
-------------------- An old man and his grandson are sitting by the fire outside the tepee, wrapped in furs and gazing into the leaping flames. High on a snowy ridge, a wolf howls at the moon and another answers from far away. Soon after, the old man removes the pipe from his mouth.
‘Grandson,’ he says. ‘There are two wolves inside you. One is white and the other is black.’
‘What are they doing there, Grandfather?’ asks the wide-eyed boy.
‘They are fighting each other,’ says the old man.
The boy considers this, then asks, ‘Why are they white and black?’
‘The white one is your love, your peace and your truth. The black one is your fear, your anger and your lies.’
The fire crackles and sparks flare in the night. The wolf on the ridge howls again and the old man puffs contentedly on his pipe. Finally, the boy says, ‘Which one will win, Grandfather?’
‘Ah,’ says the old man, removing the pipe once more. ‘The one that wins is the one that you feed.’
Edited by Wormi (05/03/16 02:20 PM)
|
brazul117
Stranger
Registered: 01/08/16
Posts: 16
Last seen: 8 years, 7 months
|
Re: BulkTEK Noob Help Plz!!! [Re: Wormi]
#23195466 - 05/06/16 12:23 PM (8 years, 8 months ago) |
|
|
So, the spore syringe used must have been shite because after a bit of experimenting on my own part I've found a BRF cake crumble to sterilized rye grains is a perfectly viable method of transitioning to bulk Tek. Regardless of what any naysayer has said I think the results speak for themselves with a 100% success rate!


Just wanted to share the info incase anyone else is attempting this methodology! (I can list the process step by step if requested)
|
|