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OfflineHEEBSHOUSE
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multiple strains in one tub ?
    #23180884 - 05/02/16 04:30 PM (8 years, 8 months ago)

Im totally new to this so hope this isnt to stupid question but here goes.

I doing the BRF Pf tek and just prepped and sterilized 20 1/2 pint jars.
I have multiple spore syringes. All diff strains . I like variety.
My question is , if my tub holds ten jars would i be able to inoculate 5 jars with strain A and another 5 jars with strain B and grow along side each other ? Or will i run into more problems then its worth.
I can wait if i have to for another grow to test others just curious.

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OfflineBigbadwooof
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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23180953 - 05/02/16 04:49 PM (8 years, 8 months ago)

I have grown two strains together before, but I think it could potentially cause problems. They are the same species, but the sexual variation in mushrooms is a lot more diverse than in humans (male/female). I think when you are growing multiple strains, they are less inclined to undergo anastomosis, and it could cause colonization problems. You probably won't end up with the results you're after either. I think one would probably dominate, and you will mostly get fruits from one strain or the other. I can't remember how mine turned out. I would suggest at least using strains from a similar region (country/geographic area).

Just my 2 Cents :wink:


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OfflineHEEBSHOUSE
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Re: multiple strains in one tub ? [Re: Bigbadwooof]
    #23180980 - 05/02/16 04:57 PM (8 years, 8 months ago)

Cool makes sense. Ill stick to one at a time

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InvisibleLocN9ne
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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23181015 - 05/02/16 05:06 PM (8 years, 8 months ago)

You can grow one cake of every single known variety side by side in the same chamber and be absolutely fine...


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OfflineHEEBSHOUSE
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Re: multiple strains in one tub ? [Re: LocN9ne]
    #23181136 - 05/02/16 05:42 PM (8 years, 8 months ago)

Is this from your own experience?

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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23181145 - 05/02/16 05:45 PM (8 years, 8 months ago)

Every grow you do from spores has 100s of strains already anyway.

Every two spores that mate is a strain. The name on a syringe is a variety not a strain. The spores have no idea that a human decided to call one spore syringe by one name and another by another name. You could mix every single cubensis variety together and its still no difference because the name on a syringe means basically nothing

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InvisibleLocN9ne
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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23181150 - 05/02/16 05:45 PM (8 years, 8 months ago)

:whathesaid:This is because they are the same species... They do not compete.


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OfflineBigbadwooof
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Re: multiple strains in one tub ? [Re: bodhisatta]
    #23181264 - 05/02/16 06:17 PM (8 years, 8 months ago)

Quote:

bodhisatta said:
Every grow you do from spores has 100s of strains already anyway.

Every two spores that mate is a strain. The name on a syringe is a variety not a strain. The spores have no idea that a human decided to call one spore syringe by one name and another by another name. You could mix every single cubensis variety together and its still no difference because the name on a syringe means basically nothing




As I said, there are hundreds of 'genders' for a mushroom species. If you start spores from two different stable 'strains' on agar, you will likely see a zone in which they resist converging with one another, at least for a period of time. They are still the same species, and can obviously be crossed, but not all strains are easily compatible with one another, because one may have a higher incidence of a particular mating gender than the other. This is why some species, when grown from MS, you don't want to shake the jar, because the mycelium will not undergo anastomosis with incompatible genetic counterparts. With Pan Cyan it is preferable to create an isolate before fruiting.

Regardless, I think this is an overly complicated answer. It sounds like you're interested in growing cakes. Therefore, you can certainly grow 10 different strains on 10 different cakes.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
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OfflineHEEBSHOUSE
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Re: multiple strains in one tub ? [Re: Bigbadwooof]
    #23181299 - 05/02/16 06:25 PM (8 years, 8 months ago)

Ya BRF cakes. So thats cool ill try a few of each then .
Thanx again guys for all the quick replies

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OfflineBigbadwooof
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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23181308 - 05/02/16 06:27 PM (8 years, 8 months ago)

Quote:

HEEBSHOUSE said:
Ya BRF cakes. So thats cool ill try a few of each then .
Thanx again guys for all the quick replies




Good luck, brother! Keep us posted on your results :wink:


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

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OfflineHEEBSHOUSE
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Re: multiple strains in one tub ? [Re: Bigbadwooof]
    #23181386 - 05/02/16 06:45 PM (8 years, 8 months ago)

Got everything sterilized. 20 half pint jars  of  2 : 1 : 1  -  Verm : BRF : Water .
Letting jars cool over night and in the morning will be making my glove box for inoculation.
Havent decided on which ones Im running yet but this is what ill have on hand to test

Amazon
B+
Alacabenzi
Cambodian
Golden Teachers

and there might be 1 or 2 more not sure.

Im really liking this new hobby and hope that I can learn as much as i can . I suffer from ADHD so theres just so much to try and go threw . So if i ask questions that have been answered b4 I aplologize its just alot easier for me to ask straight out

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OfflineBigbadwooof
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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23181416 - 05/02/16 06:51 PM (8 years, 8 months ago)

It's all good, brother.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

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Re: multiple strains in one tub ? [Re: Bigbadwooof]
    #23181451 - 05/02/16 06:59 PM (8 years, 8 months ago)

Quote:

Bigbadwooof said:
Quote:

bodhisatta said:
Every grow you do from spores has 100s of strains already anyway.

Every two spores that mate is a strain. The name on a syringe is a variety not a strain. The spores have no idea that a human decided to call one spore syringe by one name and another by another name. You could mix every single cubensis variety together and its still no difference because the name on a syringe means basically nothing




As I said, there are hundreds of 'genders' for a mushroom species. If you start spores from two different stable 'strains' on agar, you will likely see a zone in which they resist converging with one another, at least for a period of time. They are still the same species, and can obviously be crossed, but not all strains are easily compatible with one another, because one may have a higher incidence of a particular mating gender than the other. This is why some species, when grown from MS, you don't want to shake the jar, because the mycelium will not undergo anastomosis with incompatible genetic counterparts. With Pan Cyan it is preferable to create an isolate before fruiting.

Regardless, I think this is an overly complicated answer. It sounds like you're interested in growing cakes. Therefore, you can certainly grow 10 different strains on 10 different cakes.



Cubes do not have hundreds of genders. They have a 25% chance of mating and there's two sexes cubes are bifactorial. Species like s.commune have 1000s of sexes

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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23181459 - 05/02/16 07:01 PM (8 years, 8 months ago)

You absolutely can grow multiple varieties and strains of cakes in the same FC.

You see multiple strains growing together every time you grow with MS.

The ability for spores to mate with each other is not limited to one being called GT and the other being called B+. Spores from each can still mate with the other.

The ability for multiple strains to grow together is the same whether from a single print from a single mushroom or from two different prints from two different varieties.

In any case, it's cakes in a SGFC, so it doesn't matter, they are just growing in the same FC.


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InvisibleLocN9ne
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Re: multiple strains in one tub ? [Re: SpitballJedi]
    #23181476 - 05/02/16 07:04 PM (8 years, 8 months ago)

Quote:

HEEBSHOUSE said:
Got everything sterilized. 20 half pint jars  of  2 : 1 : 1  -  Verm : BRF : Water .
Letting jars cool over night and in the morning will be making my glove box for inoculation.
Havent decided on which ones Im running yet but this is what ill have on hand to test

Amazon
B
Alacabenzi
Cambodian
Golden Teachers

and there might be 1 or 2 more not sure.

Im really liking this new hobby and hope that I can learn as much as i can . I suffer from ADHD so theres just so much to try and go threw . So if i ask questions that have been answered b4 I aplologize its just alot easier for me to ask straight out



Fuck a glove box... Make a SAB

https://www.shroomery.org/forums/showflat.php/Number/20048771


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OfflineHEEBSHOUSE
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Re: multiple strains in one tub ? [Re: LocN9ne]
    #23181509 - 05/02/16 07:13 PM (8 years, 8 months ago)

Just looked at what SAB was. Thats actually what im making for inoculation time

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OfflineBigbadwooof
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Re: multiple strains in one tub ? [Re: bodhisatta]
    #23181558 - 05/02/16 07:24 PM (8 years, 8 months ago)

Quote:

bodhisatta said:
Quote:

Bigbadwooof said:
Quote:

bodhisatta said:
Every grow you do from spores has 100s of strains already anyway.

Every two spores that mate is a strain. The name on a syringe is a variety not a strain. The spores have no idea that a human decided to call one spore syringe by one name and another by another name. You could mix every single cubensis variety together and its still no difference because the name on a syringe means basically nothing




As I said, there are hundreds of 'genders' for a mushroom species. If you start spores from two different stable 'strains' on agar, you will likely see a zone in which they resist converging with one another, at least for a period of time. They are still the same species, and can obviously be crossed, but not all strains are easily compatible with one another, because one may have a higher incidence of a particular mating gender than the other. This is why some species, when grown from MS, you don't want to shake the jar, because the mycelium will not undergo anastomosis with incompatible genetic counterparts. With Pan Cyan it is preferable to create an isolate before fruiting.

Regardless, I think this is an overly complicated answer. It sounds like you're interested in growing cakes. Therefore, you can certainly grow 10 different strains on 10 different cakes.



Cubes do not have hundreds of genders. They have a 25% chance of mating and there's two sexes cubes are bifactorial. Species like s.commune have 1000s of sexes




Hmm, I just looked into it further, and it seems you are correct. I suppose you aren't inclined to run into this problem with Cubensis.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

Edited by Bigbadwooof (05/02/16 07:24 PM)

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OfflineHEEBSHOUSE
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Re: multiple strains in one tub ? [Re: Bigbadwooof]
    #23181583 - 05/02/16 07:29 PM (8 years, 8 months ago)

Prob going to end up inoculating 4 jars per strain i have. So with 20 jars ill be able to test all 5 listed. Now i have to start looking at doing my own prints or syringes or whatever it is you guys do to keep you grows going without having to re order for every grow.

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OfflineBigbadwooof
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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23181597 - 05/02/16 07:32 PM (8 years, 8 months ago)

Quote:

HEEBSHOUSE said:
Prob going to end up inoculating 4 jars per strain i have. So with 20 jars ill be able to test all 5 listed. Now i have to start looking at doing my own prints or syringes or whatever it is you guys do to keep you grows going without having to re order for every grow.




That sounds like a fun way to get started for sure!


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

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OfflineHEEBSHOUSE
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Re: multiple strains in one tub ? [Re: Bigbadwooof]
    #23183329 - 05/03/16 08:39 AM (8 years, 8 months ago)



20 jars sterilized and inoculated. This is where they will sit until ready for birth or garbage , which ever way it goes lol.

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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23183697 - 05/03/16 10:24 AM (8 years, 8 months ago)

:thumbup:

Remove the tape. The dry verm layer is your contamination barrier. Leaving the holes covered can cause stalling later.


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OfflineBigbadwooof
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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23183730 - 05/03/16 10:31 AM (8 years, 8 months ago)

:freewilly:


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

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OfflineHEEBSHOUSE
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Re: multiple strains in one tub ? [Re: SpitballJedi]
    #23183869 - 05/03/16 11:06 AM (8 years, 8 months ago)

Its breathable tape and i punctured holes threw when inoculating. So the holes arent covered. Just left tape on for labels

Edited by HEEBSHOUSE (05/03/16 11:08 AM)

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OfflineHEEBSHOUSE
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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23183929 - 05/03/16 11:24 AM (8 years, 8 months ago)



got paranoid since sggestion about tape so i yanked it. Lol.

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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23185287 - 05/03/16 04:51 PM (8 years, 8 months ago)

:thumbup:


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OfflinePsilosoulful

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Re: multiple strains in one tub ? [Re: HEEBSHOUSE]
    #23185862 - 05/03/16 06:57 PM (8 years, 8 months ago)

Quote:

HEEBSHOUSE said:


got paranoid since sggestion about tape so i yanked it. Lol.



Yep, you don't need it

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