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Offlinegrowbit
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Registered: 10/29/10
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Last seen: 7 months, 22 days
Successfully cleaned a dirty spore syringe!
    #23179200 - 05/02/16 08:05 AM (8 years, 8 months ago)

So I ordered a spore syringe from a non-sponsor, and (surprise, surprise) it was infested with bacteria. Without testing it, I decided to knock up 6 quart jars as well as two liquid cultures, and practically all of them got contaminated with Bacillus.

I even tried isolating the spores on agar, but they wouldn't even germinate after two weeks after I placed a drop of syringe water on 6 agar plates.

I was just about to give up and throw all my quart jars away when I saw about 1 cubic inch of healthy mycelium growing amidst the contaminated jar. I decided to try isolate a single healthy grain of myc by very carefully opening the jar in a glove box and taking a scalpel to pick up a single grain out of the jar and place it onto an agar plate. I repeated this for about 5 agar plates just for redundancy.

4 of the 5 plates became hopeless contaminated and did not grow, and one plate grew some healthy myc that I am going to isolate further and then transfer to a grain master for G2G down the line.

Its been a frustrating journey but it feels good having finally conquered the contaminants with a bit of luck and good mycology technique. I thought I'd share the story in case it helped anyone struggling with contaminated syringes!

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InvisibleMad Season
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Registered: 09/16/12
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Re: Successfully cleaned a dirty spore syringe! [Re: growbit]
    #23179918 - 05/02/16 12:08 PM (8 years, 8 months ago)

:rockon:

Contaminated spores suck, but 2 weeks on bacterial agar isn't long enough. After 3-4 weeks I'd toss. It's hard for myc to germinate on bacteria


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