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Offlineglbggg
Gentleman

Registered: 04/22/16
Posts: 102
Last seen: 8 years, 4 months
Question regarding mono tubs and the amount of flushes
    #23178145 - 05/01/16 10:27 PM (8 years, 8 months ago)

sifting through all the information/misinformation and I'm honestly a little stumped..
I'm looking to start 3 test bins after my first grow (with isolated genetics)
at spawn ratios of 1:1, 1:2 and 1:3 to document the different colonization times as well as the effects (if any) on its yields.

The ONLY thing i'm still not 100% sure about.. is the amount of FLUSHES to expect/prepare for.

does the 1:1 bin require that I expect more flushes from it? or should I stay safe and go for 2? I've read lots of shit people say anymore than 2 and ur pushing it?

ive also seen some teks for SINGLE flush mono's

then I've seen others claim 3-4 flushes were harvested easily

What i'm scared of is throwing away a tub prematurely when it could potentially yield me something (i live in a big city and no backyard to bury cakes in)

I'm probably going to just push for 2 flushes per mono, regardless of spawn.. cus dunking seems easy enough after a first flush, although I read RR say u shud just splash some water on the sides and underneath, not directly on it, for 20minutes or less even? then drain excess and put back into FC (please tell me if this is me making things up and if its even a good method)

just curious to hear your guys opinions.. i feel this is one of those topics that varies from individual to individual

i read on another forum.. one guy claiming he could dual-flush (2 flush) almost a dry pound, from every bin with  8 quart spawn to 1 brick of coir/verm/gypsum. he said he got monster sized fruits 9-10 dry oz first flush dry 4-5 the 2nd from all 3 of his tubs, but he didn't say if he went to 3rd flush or not, or a 4th

so what are all of your opinions? I don't really know WHY the sub gets contam'd after the first and second flushes.. I'm trying to grasp it all from search to search but its a lot.. i know FAE is super important as molds don't grow when there is moving oxygen present, i know casing is NOT necessary and may even be detrimental to the tub.. Im just having so much doubt and not being confident.. like the confidence i had when i first noc'd up my grains.. but after losing 5/6 grain jars to improper moisture content (i had a very small strainer that i was overloading with rye because i have demands hahaha *im dumb*) now I'm having doubts... from healthy grain jars, to mixing sub, harvesting and most importantly the after harvest priorities. FLUSHES
I just don't want to get a contam in my tubs
take my jars! take all the jars you want
just please don't take my tubs!!! :3
I got another spore syringe, a new PC, more quart sized mason jars, A WINDOW SCREEN to strain (its so freaking good!) and noc'd up another 6 jars (didn't use the whole spore solution of course lol, very small amounts because I'm so scared of more bacterial contams and too much spore solution can lead to improper moisture right? cus these 6 new jars are perfect.. if anything a bit dry, but theres no condensation on the inside after the PC cool down, i don't see what i saw in the other jars at all so I'm confident that these will AT LEAST full colonize and make it to the next step. please someone just restore my confidence needed for the next step haha, the prepping of sub I'm confident with, i got my oven bags ready as well, all that, gonna pasteurize and then bake for the highest chance of NO contams, but flushes.. after i cut/harvest the first flush, would i just apply the basic "soak" i explained above, and go second flush then toss?



like i feel like the reason people get contams after first and second flush is because:
They didn't let it consolidate properly in colonization phase
not enough FAE
or damaging the substrate below when harvesting (not cutting and ripping/tearing) thus exposing grain
thats just 3 of the things I've gathered from searching post to post.. anymore would help a lot, at least if i could just apply my knowledge of every cause i would be able to figure out how to combat it.

anyways Id love to hear anything u guys have to say so please get back to me

I'm thinking once I'm a veteran i may even look into joining a bunch of teks as well as information i gather from experience and write-up a GREAT BIG TEK, that acts as an encyclopaedia haha.. that would be my way of giving back to the community
thanks guys!

Edited by glbggg (05/01/16 11:20 PM)

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Invisibledankington
The Stranger
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Folding@home Statistics
Registered: 03/14/15
Posts: 4,577
Loc: 8te
Re: Question regarding mono tubs and the amount of flushes [Re: glbggg]
    #23178278 - 05/01/16 10:59 PM (8 years, 8 months ago)

:bongload: for this WOT

Your spawn ratio will not necessarily affect your yield, nor colonization speed very noticeably IME. What's important is that it's 1:1-1:4. There are a few reasons to change spawn ratio. For example, when spawn appears slightly questionable, but definitely viable, a 1:1 ratio will be more likely to be successful. At least for a flush, maybe 2. I typically consider myself lucky with 2 flushes and place the tub somewhere else. If something else comes up great, otherwise I go clean it outside.

You say you have isolated genetics, so I take it you have been making transfers from your original plate? If you really have clean isolates, I don't see why you wouldn't be able to pull out 3-4 flushes with properly prepared materials.

But then you say you are dropping spore solution to your grains? That's not an isolate, that's multispore. It's also a gamble and will drop your chances of multiple flushes.

A casing layer, though not necessary can be extremely helpful also. It creates a microclimate over your substrate which can increase pinning.

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Offlineglbggg
Gentleman

Registered: 04/22/16
Posts: 102
Last seen: 8 years, 4 months
Re: Question regarding mono tubs and the amount of flushes [Re: dankington]
    #23178320 - 05/01/16 11:15 PM (8 years, 8 months ago)

yeah I will be doing that method the second time around.. from the 1 jar that I had that survived from initial innoc I'm gonna use as master for 10 and spawn 2 tubs and see what i get and clone, I'm also waiting for spores to germ on my agar, I'm just eager to FRUIT/HARVEST so me and my friends can go to the park and munch, ill also be cloning from whatever I yield. I'm sorry if I've confused you
I'm asking the question for my later method just went off topic talking about lack of confidence going into the next stages haha
so the amount of flushes is all dependant on GENETICS? lmao
ok wow this is a new 1 i haven't heard yet..... I've heard of water/dunking = more flushes
I've heard more spawn/nutes to sub = more flushes..
but GENETICS for more flushes? are you sure?

also what do you mean the spawn looks questionable?? how can spawn look questionable if its fully colonized?


--------------------
goodbye cruel world

Edited by glbggg (05/01/16 11:22 PM)

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Invisibledankington
The Stranger
Male User Gallery


Folding@home Statistics
Registered: 03/14/15
Posts: 4,577
Loc: 8te
Re: Question regarding mono tubs and the amount of flushes [Re: glbggg]
    #23178348 - 05/01/16 11:25 PM (8 years, 8 months ago)

I think in theory, if everything is clean, and there's still nutrition in your substrate, you could keep getting flushes. But this isn't realistic because the stress the substrate goes through producing each flush makes it vulnerable.

You just learn what to look for. There's tons of information on contaminants. Mycelium will grow OVER a contaminant in many cases, but it in no way means it's not still there.

Here, take a look at this:
https://www.shroomery.org/forums/showflat.php/Number/17231150#17231150

But there's tons of good info out there.

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OfflinePsilicon
Really Nice Guy

Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
Re: Question regarding mono tubs and the amount of flushes [Re: dankington]
    #23178705 - 05/02/16 02:44 AM (8 years, 8 months ago)

Like dankington says, the number of flushes is dependent on the genetics you have in your substrate, the amount of nutrition and water the sub can draw from to keep healthy and keep up the fight against Trichoderma, the spore load of Trichoderma in your grow space and probably most importantly the amount of energy it's expended on its most recent flush. Just keep an eye on your sub for signs of contamination.  If you're vigilant you can catch the mold before it turns green.


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