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Offlinetombosley8
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Syringe sterilization in PC/Sterilizer
    #23177834 - 05/01/16 08:50 PM (8 years, 8 months ago)

I have a bunch of 60 ml syringes that I reuse by pcing wrapped in foil. I usually have the syringe full of air so the plunger is fully out(a little less so it can fit).

Should I just have the plunger all the way down so I can fit more in pc and easier to work with in sab.

I was thinking that it was safer to have it open so the inside of the tube was exposed as little as possible...

but when I come to think of it the plastic on the plunger is exposed and close to my hands and then is pushed down into the tube possibly bringing in contams before I suck up my LC.

Just another step that could maybe be another vector, right?


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Edited by tombosley8 (05/01/16 09:08 PM)

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Offlinehampiri
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Re: Syringe sterilization in PC/Sterilizer [Re: tombosley8]
    #23177921 - 05/01/16 09:09 PM (8 years, 8 months ago)

I usually do them all the way down but I don't think it really matters. The rubber plunger will catch any contaminants that somehow made their way into the SAB air in it's grooves. I would go with the convenience factor of being able to do more at once.


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InvisibleJosex
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Re: Syringe sterilization in PC/Sterilizer [Re: tombosley8]
    #23177970 - 05/01/16 09:24 PM (8 years, 8 months ago)

Quote:

tombosley8 said:
Just another step that could maybe be another vector, right?




How do you make your LC? Because a LC from MS would be a huuuuge contam vector all by itself.

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InvisiblePirateSwazey
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Re: Syringe sterilization in PC/Sterilizer [Re: Josex]
    #23178095 - 05/01/16 10:05 PM (8 years, 8 months ago)

You're thinking too hard bro :smile:

If you're that worried about contamination why don't you just do agar straight to grain?


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Invisibleazur
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Re: Syringe sterilization in PC/Sterilizer [Re: tombosley8]
    #23178134 - 05/01/16 10:23 PM (8 years, 8 months ago)

Yea, I have the plunger pushed in all the way when I sterilize syringes.
But either way, your hands should be clean and sanitized when you are working.


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Invisiblemaddchef
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Re: Syringe sterilization in PC/Sterilizer [Re: azur]
    #23178354 - 05/01/16 11:27 PM (8 years, 8 months ago)

flame sterilize your hands before handling


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Invisibleazur
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Re: Syringe sterilization in PC/Sterilizer [Re: maddchef]
    #23178370 - 05/01/16 11:30 PM (8 years, 8 months ago)

Flame sterilize your cock before inoculating your bitch


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InvisiblebodhisattaMDiscordReddit
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Re: Syringe sterilization in PC/Sterilizer [Re: azur]
    #23178398 - 05/01/16 11:38 PM (8 years, 8 months ago)

:underage:

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OfflineKizzle
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Re: Syringe sterilization in PC/Sterilizer [Re: bodhisatta]
    #23178671 - 05/02/16 02:21 AM (8 years, 8 months ago)

Quote:

Just another step that could maybe be another vector, right?



In theory but they're designed with a pretty effective seal. I seem to recall an experiment where they used ungloved hands and soaked sterile water in and out like 30 times and the seal was enough to keep it sterile so I wouldn't worry too much.


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OfflineFreeWorldOrder
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Re: Syringe sterilization in PC/Sterilizer [Re: Kizzle]
    #23179341 - 05/02/16 08:55 AM (8 years, 8 months ago)

I just draw up boiling water and squirt it out a few times then leave the final draw in then flame the needle.

The syringes I have now have been used many times, the graduations are worn off.

They have never seen a PC so far and contams haven't been an issue as of yet...

PC'ing syringes is fine too, just haven't found it absolutely necessary and the boiling water method only takes a couple minutes.

Though I mostly do agar work now, I will occasionally do a couple dozen BRF cakes.


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Edited by FreeWorldOrder (05/02/16 09:01 AM)

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