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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 6 months, 13 days
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Syringe sterilization in PC/Sterilizer
#23177834 - 05/01/16 08:50 PM (8 years, 8 months ago) |
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I have a bunch of 60 ml syringes that I reuse by pcing wrapped in foil. I usually have the syringe full of air so the plunger is fully out(a little less so it can fit).
Should I just have the plunger all the way down so I can fit more in pc and easier to work with in sab.
I was thinking that it was safer to have it open so the inside of the tube was exposed as little as possible...
but when I come to think of it the plastic on the plunger is exposed and close to my hands and then is pushed down into the tube possibly bringing in contams before I suck up my LC.
Just another step that could maybe be another vector, right?
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Edited by tombosley8 (05/01/16 09:08 PM)
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hampiri
Time Traveller



Registered: 04/12/16
Posts: 52
Loc: Essos
Last seen: 8 years, 5 months
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Re: Syringe sterilization in PC/Sterilizer [Re: tombosley8]
#23177921 - 05/01/16 09:09 PM (8 years, 8 months ago) |
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I usually do them all the way down but I don't think it really matters. The rubber plunger will catch any contaminants that somehow made their way into the SAB air in it's grooves. I would go with the convenience factor of being able to do more at once.
-------------------- "Trust the fungus..." -Mario
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,999
Loc:
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Re: Syringe sterilization in PC/Sterilizer [Re: tombosley8]
#23177970 - 05/01/16 09:24 PM (8 years, 8 months ago) |
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Quote:
tombosley8 said: Just another step that could maybe be another vector, right?
How do you make your LC? Because a LC from MS would be a huuuuge contam vector all by itself.
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Syringe sterilization in PC/Sterilizer [Re: Josex]
#23178095 - 05/01/16 10:05 PM (8 years, 8 months ago) |
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You're thinking too hard bro 
If you're that worried about contamination why don't you just do agar straight to grain?
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Syringe sterilization in PC/Sterilizer [Re: tombosley8]
#23178134 - 05/01/16 10:23 PM (8 years, 8 months ago) |
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Yea, I have the plunger pushed in all the way when I sterilize syringes. But either way, your hands should be clean and sanitized when you are working.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Syringe sterilization in PC/Sterilizer [Re: azur]
#23178354 - 05/01/16 11:27 PM (8 years, 8 months ago) |
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flame sterilize your hands before handling
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Syringe sterilization in PC/Sterilizer [Re: maddchef]
#23178370 - 05/01/16 11:30 PM (8 years, 8 months ago) |
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Flame sterilize your cock before inoculating your bitch
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Syringe sterilization in PC/Sterilizer [Re: azur]
#23178398 - 05/01/16 11:38 PM (8 years, 8 months ago) |
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
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Re: Syringe sterilization in PC/Sterilizer [Re: bodhisatta]
#23178671 - 05/02/16 02:21 AM (8 years, 8 months ago) |
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Quote:
Just another step that could maybe be another vector, right?
In theory but they're designed with a pretty effective seal. I seem to recall an experiment where they used ungloved hands and soaked sterile water in and out like 30 times and the seal was enough to keep it sterile so I wouldn't worry too much.
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
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Re: Syringe sterilization in PC/Sterilizer [Re: Kizzle]
#23179341 - 05/02/16 08:55 AM (8 years, 8 months ago) |
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I just draw up boiling water and squirt it out a few times then leave the final draw in then flame the needle.
The syringes I have now have been used many times, the graduations are worn off.
They have never seen a PC so far and contams haven't been an issue as of yet...
PC'ing syringes is fine too, just haven't found it absolutely necessary and the boiling water method only takes a couple minutes.
Though I mostly do agar work now, I will occasionally do a couple dozen BRF cakes.
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Edited by FreeWorldOrder (05/02/16 09:01 AM)
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