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bastard4life
Travel Agent



Registered: 03/12/11
Posts: 955
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what wrong with my lcs
#23175348 - 05/01/16 08:31 AM (8 years, 8 months ago) |
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OK I'm confused. I'm thinking my lcs aren't working out right. I mixed 1.5 tablespoons of Great Lakes brand premium golden honey into 750ml of water. That's about 32 grams of honey somewhere around 4.5 grams per 100ml. I knocked up some ms mazatapac and ape lc the same day as I inoculated some pf cakes. Pf cakes all have good growth. No growth in jars at all. I made 7 pint lc jars from the 750 ml and only inoculated 2 as I haven't gotten to the others yet but all 7 jars look the same as when I pced them. I'm wondering if gas exchange is an issue. I have syringe filters on all 7 jars. I used to do the baby bottle tek back in the day and had a polyfil filled syringe I'd use to break the vacuum and allow oxygen in. Was I supposed to add fresh air into the lc? Can I take a syringe and PC it full of air and inject it into the liquid?
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Groo
sola dosis facit venenum



Registered: 12/06/14
Posts: 2,313
Last seen: 5 months, 30 days
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Going MS to LC is a NO NO. That is your problm. You go clean isolate to LC not nasty MS to lc. never gonna work unless it does which is rare enough.
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hampiri
Time Traveller


Registered: 04/12/16
Posts: 52
Loc: Essos
Last seen: 8 years, 5 months
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Re: what wrong with my lcs [Re: Groo]
#23175421 - 05/01/16 09:05 AM (8 years, 8 months ago) |
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That's odd, I've never had trouble with MS liquid culture; it's just a bit slower to colonize is all and I get 4-5 blobs per 1/2 pint jar. I pick the healtheist looking blob (I don't stir my LC) and suck it up with a 14ga needle.
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GaiaSporeworks
Stranger


Registered: 10/31/15
Posts: 52
Last seen: 7 years, 8 months
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Re: what wrong with my lcs [Re: hampiri]
#23175454 - 05/01/16 09:19 AM (8 years, 8 months ago) |
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I used to do MS to LC. It was impossible to keep the cultures clean. I'm throwing them all out.
Since then I've started a nice agar collection with a 0% contamination rate. Using a flowhood helps a lot, but if you use a SAB properly, you can achieve clean transfers and inoculations no problem. I love agar because you can easily expand your cultures, convert agar to LI with a jar of sterile water and a sterile syringe, clone mushroom specimen by taking tissue samples, and one of my favorite parts of agar is the ability to select/transfer sectors based on visual traits and growth speed to expand the best set of genetics a substrain can give me. It's been fun as hell for me!
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: what wrong with my lcs [Re: Groo]
#23175477 - 05/01/16 09:29 AM (8 years, 8 months ago) |
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Quote:
Groo said: Going MS to LC is a NO NO. That is your problm. You go clean isolate to LC not nasty MS to lc. never gonna work unless it does which is rare enough.
You don't have to get an isolate to inoculate an LC. MS will do just fine IF you do not germinate your spores inside the LC. Normally, one would germinate the spores on agar and after making multiple transfers to make sure the mycelium is clean, he can inoculate an LC with a wedge. This is still considered as MS because there are multiple strains inside your LC, any grow resulting from that will also be considered as a MS grow.
Going from spores to LC is risky because LC is really susceptible to contams and no syringe or print is ever 100% clean. Reputable sponsors are able to get very clean syringes but again, never 100% clean. The risk with making an LC from spores is that a contam can actively grow and stay hidden and any inoculations resulting from that LC will get that contam. With jars, one can more easily spot a contam and trash it, leaving other healthy jars for your grow.
IMHO, spores shouldn't even be used to inoculate jars as it is a waste of money and riskier than agar. Why would I keep buying syringe after syringe to inoculate jars when 1 drop of that spore suspension can be kept and grown indefinitely on agar.
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bastard4life
Travel Agent



Registered: 03/12/11
Posts: 955
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Since its a no no its a good thing I didn't put all my eggs in one basket. I got some pf colonizing now and just poured ten plates. I'm putting an oyster wedge on one a shiitake wedge an another and a drop of ape on a third. That leaves me with 7 plates to use on ape as the other two are isolates already of tried and true fruiters.
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bastard4life
Travel Agent



Registered: 03/12/11
Posts: 955
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Quote:
Supalemonhaze said:
Quote:
Groo said: Going MS to LC is a NO NO. That is your problm. You go clean isolate to LC not nasty MS to lc. never gonna work unless it does which is rare enough.
You don't have to get an isolate to inoculate an LC. MS will do just fine IF you do not germinate your spores inside the LC. Normally, one would germinate the spores on agar and after making multiple transfers to make sure the mycelium is clean, he can inoculate an LC with a wedge. This is still considered as MS because there are multiple strains inside your LC, any grow resulting from that will also be considered as a MS grow.
Going from spores to LC is risky because LC is really susceptible to contams and no syringe or print is ever 100% clean. Reputable sponsors are able to get very clean syringes but again, never 100% clean. The risk with making an LC from spores is that a contam can actively grow and stay hidden and any inoculations resulting from that LC will get that contam. With jars, one can more easily spot a contam and trash it, leaving other healthy jars for your grow.
IMHO, spores shouldn't even be used to inoculate jars as it is a waste of money and riskier than agar. Why would I keep buying syringe after syringe to inoculate jars when 1 drop of that spore suspension can be kept and grown indefinitely on agar.
That was my real plan honestly but I was nervous about my lc sterility and decided to make up some just to sit around and see if it they stay sterile. I figured if they sat around for a few weeks while i wait for the pf to fruit for a clone and put a drop on agar then I'd know my LCS would be good. I've heard mixed things about the syringe filters and I don't know what micron they are.
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Groo
sola dosis facit venenum



Registered: 12/06/14
Posts: 2,313
Last seen: 5 months, 30 days
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That's odd, I've never had trouble with MS liquid culture;
Then you my brother must have gotten sores from a trusted source that the clt was REALLY REALLY REALLY GOOD!! Good 4 you and keep buying from them! Home depot with ores? Their my fav!
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