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InvisibleKush_Zombie
smug piece of shit
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Registered: 10/22/14
Posts: 4,793
Contaminated Agar?
    #23175193 - 05/01/16 07:02 AM (8 years, 8 months ago)

Tried cloning with pins for the first time...I know there's possibly trich on the pin itself but is all this white shit around it a contam? I'm really hoping it's just from water being knocked down.....

I picked the pins with tweezers and then took them to my work area and dropped them on my agar plate inside of a SAB. Almost all of my clones look like this...

Will the mycelium from the pins overrun them?



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InvisibleScrimshaw
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Registered: 06/27/14
Posts: 793
Re: Contaminated Agar? [Re: Kush_Zombie]
    #23175260 - 05/01/16 07:48 AM (8 years, 8 months ago)

That's a lot of bacteria. The myc may over run it (doubtful) but even if it did the bacteria will most likely still be in there. If you have another clean plate, I'd rather just put a new pin on that.

Otherwise, you could use the tiny bit of myc you have in the center to take a transfer. But assume it's contaminated and make multiple transfers from that until you know it's clean.

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InvisibleKush_Zombie
smug piece of shit
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Registered: 10/22/14
Posts: 4,793
Re: Contaminated Agar? [Re: Scrimshaw]
    #23175267 - 05/01/16 07:51 AM (8 years, 8 months ago)

I'm hurrying up and prepping another 16 plates today. I have a really nice mushroom that's almost fully grown so I'm just going to take a lot of transfers from it and one other mushroom.

Thanks for the reply.


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.

Extras: Filter Print Post Top
InvisibleScrimshaw
Sponsored by Sterilite
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Registered: 06/27/14
Posts: 793
Re: Contaminated Agar? [Re: Kush_Zombie]
    #23175270 - 05/01/16 07:53 AM (8 years, 8 months ago)

Sounds like a plan. If you rip open the mushroom in the sab and put material from the inner stem on the agar you'll have a better chance of clean myc. Good luck.

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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Contaminated Agar? [Re: Scrimshaw]
    #23175502 - 05/01/16 09:37 AM (8 years, 8 months ago)

Sandwich that bitch, that will clean it up.

Heat up your scalpel to red hot and make a hole in the middle of a new plate, take that transfer and put it in the hole. Then, reheat the scalpel so you won't make a bacterial mess everywhere it touches and cut a square of agar from the plate to cover the clone transfer. Unlike mycelium, bacteria cannot grow through agar so any mycelium that grows on top of the square covering the original transfer will be clean. Scrape some mycelium off being careful not to go all the way down or you will re-contaminate it again. Once should do it but doing it twice will give you better peace of mind, especially if you never done this before.

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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
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Re: Contaminated Agar? [Re: Supalemonhaze]
    #23175757 - 05/01/16 10:32 AM (8 years, 8 months ago)

:whathesaid: sandwich tek is made for this.

Personally I've had even more bacterial plates than that, I let it grow about a dime size, and transferred about 6 transfers before being able to get a clean transfer without bacteria. Sandwich tek is much quicker, but you can save a bacterial culture with pure transfers too.


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