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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Innoc'd with 9mo old syringe, not seeing growth
#23173693 - 04/30/16 06:47 PM (8 years, 8 months ago) |
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Hey everyone. May have seen me post before. Relatively familiar with PF tek. I used a syringe to knock some containers up the other day that I ordered back in.... August, maybe? I've shot up 6 syringes in the past, so I mostly know what to look for. Doing the best I can not to disturb my containers, but, I'm not seeing a thing other than very small condensation drops from the innoc points. Which leads me to believe all may be good, but I shot them.... 5 days ago, IIRC, and I'm not seeing any mycelium growth to the naked eye.
I know, patience patience patience, but I'm very concerned something isn't right. I used a variation recipe, was 6 cups of BRF, 2 Verm, and 2 water. Wondering if the substrate may be too dry.
I don't really know what I'm asking. I guess just if the chances are decent that a 9 month old syringe, never refrigerated, may be dead?
Thanks everyone!
Strain is golden teacher, BTW. Not sure if they colonize slower than others..
-------------------- KSSS And PE WBS.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23173710 - 04/30/16 06:54 PM (8 years, 8 months ago) |
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I'm sure you know this, but wait it out. I wait 3 weeks after inoc before throwing them out and saying spores are defective.
May I suggest trying agar in the meantime. Forcing germination (which you might need to do if you wanna salvage this syringe), almost infinite supply of myc from a single drop (never needing to go back to spores again), and culture clean up is the perks of agar
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23173711 - 04/30/16 06:54 PM (8 years, 8 months ago) |
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Syringe age is fine.
Five days isn't long enough to worry or make a thread about
Your pf recipe is wrong.
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AusMush



Registered: 10/24/15
Posts: 1,074
Loc: Somewhere in the Aussie s...
Last seen: 3 months, 7 days
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: bodhisatta]
#23173723 - 04/30/16 06:57 PM (8 years, 8 months ago) |
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If anything you should be using 6 Vermiculite to 2 brf ... That mixture you have made would literally be like glue and wet cereal.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: AusMush]
#23173753 - 04/30/16 07:05 PM (8 years, 8 months ago) |
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The recipe is 1 part brf, 1 part water, 2 parts verm. Anything else is fail. Bleeding jesus we have seen pretty much every combination and ratio of those 3 ingredients and yet to see anyone top the tek as it stands.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: AusMush]
#23173755 - 04/30/16 07:05 PM (8 years, 8 months ago) |
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Thanks everyone. Gonna get a pressure cooker soon. The Walmart, 16qt presto, for 60 bucks a decent cooker?
-------------------- KSSS And PE WBS.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23173759 - 04/30/16 07:07 PM (8 years, 8 months ago) |
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Yes. It will serve your purpose. Be sure that when you cook grain in it you follow the teks. Good luck.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Pastywhyte]
#23173767 - 04/30/16 07:09 PM (8 years, 8 months ago) |
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Apologize to offend anyone for deviating from the tek. Saw someone on here use that recipe, and seemed logical more nutrition wouldn't hurt anything. I may be mistaken, time will see.
-------------------- KSSS And PE WBS.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23173779 - 04/30/16 07:12 PM (8 years, 8 months ago) |
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Believe it or not brf cakes are actually too nutritious. That's why we need to consolidate them. More nutes is not always better.
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AusMush



Registered: 10/24/15
Posts: 1,074
Loc: Somewhere in the Aussie s...
Last seen: 3 months, 7 days
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Pastywhyte]
#23173815 - 04/30/16 07:26 PM (8 years, 8 months ago) |
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No offence taken where all here to help bro you may get away this time but do some wbs with your pc and you'll have shrooms comin out your ass in under 2 months
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omgwtfstfu
Stranger

Registered: 10/27/15
Posts: 172
Last seen: 8 months, 17 days
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: AusMush]
#23173875 - 04/30/16 07:42 PM (8 years, 8 months ago) |
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The best way to test a syringe is agar. The best way to start from spores is also onto agar. Makes some pastyplates and never look back. It's not hard. It's cheap to get into and it's the foundation of mycology. Starting with agar is the best move for anyone that has a PC.
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AusMush



Registered: 10/24/15
Posts: 1,074
Loc: Somewhere in the Aussie s...
Last seen: 3 months, 7 days
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: omgwtfstfu]
#23173893 - 04/30/16 07:47 PM (8 years, 8 months ago) |
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Agar is great , it's just getting the pH, consistency and nutrients right
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theGODSmademedoit

Registered: 03/04/16
Posts: 516
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: AusMush]
#23174921 - 05/01/16 01:49 AM (8 years, 8 months ago) |
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how is it more nutes at verm 3 to 1 brf vs 2 to 1 ? Its not an automatic fail though just dryer cakes
Edited by theGODSmademedoit (05/01/16 01:51 AM)
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AusMush



Registered: 10/24/15
Posts: 1,074
Loc: Somewhere in the Aussie s...
Last seen: 3 months, 7 days
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Read the God damn post dickweed he said he used MORE brf than Vermiculite this is MORE nutrients...
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AusMush



Registered: 10/24/15
Posts: 1,074
Loc: Somewhere in the Aussie s...
Last seen: 3 months, 7 days
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And I was talking about agar not brf cakes..... Fuckin reeeead
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theGODSmademedoit

Registered: 03/04/16
Posts: 516
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: AusMush]
#23175017 - 05/01/16 03:30 AM (8 years, 8 months ago) |
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Yea my bad wtf Fail
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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I made 8 half pints and 4 quarter pints last night, with the correct ratio. 2 Verm 1 BRF 1 water. I ran out of BRF at about a cup, so I ground up a cup of organic brown rice. I used 4 Verm, 2 BRF, 2 water, and it made enough for all my containers. Even had a little extra to go back and add a little more to the jars.
Steam sterilizing now. Gonna use my last syringe I'm hoping by this grow, I'll learn how to use agar and use my spore print a fellow member sent me.
Kinda sad to use my last syringe though. Transkei strain. Hopefully it'll be good 
Thanks everyone!
-------------------- KSSS And PE WBS.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23175519 - 05/01/16 09:41 AM (8 years, 8 months ago) |
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Save a drop in the syringe and put it to agar. That's all I even use to start a grow from a syringe. A drop.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Pastywhyte]
#23175972 - 05/01/16 11:32 AM (8 years, 8 months ago) |
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Trying to not make an unnecessary thread, so I'll ask here. What's pre poured plates? I want to start agar, and I want the "easiest" route for a beginner. Also, pre made mix sounds like a good idea. Can someone link me to the best place to get these items please?
Thanks!
-------------------- KSSS And PE WBS.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23176100 - 05/01/16 12:11 PM (8 years, 8 months ago) |
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first link in Pasty's sig... its easy af.. so easy that it is even in the name of the link.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Patsyblack
5% er
Registered: 05/01/16
Posts: 183
Last seen: 8 years, 7 months
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: mupetmower]
#23176202 - 05/01/16 12:42 PM (8 years, 8 months ago) |
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hell yeah i love patsy plates
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Patsyblack]
#23177115 - 05/01/16 05:20 PM (8 years, 8 months ago) |
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ME TOO!.. wait, is this an alt PastyWhyte account? hmmmm
anyways, i just went to the dollar store and found sooo many amazing pasty plates to use, some where 5 for a dollar and some were 10 for a dollar. one says pp5 on the bottom, so it should be alright in the PC, but the other doesnt say pp5.. so hopefully those will be ok in the PC, too, because they are amazingly perfect and the 10 for a dollar pack. they are kinda small, and clear with no obstructions on the sides, with a white cap, that opens easily, but also seals well. so excited to see if they work. gonna make some plates tonight just to see. if so, im going to go back and buy every single pack they have. and if not, im going back anyways to buy all the rest of the 5 for a dollar packs of the others which are basically glad mini knock offs, but with a clear side that doesnt have that shit on the bottom of the sides =]
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: mupetmower]
#23177160 - 05/01/16 05:30 PM (8 years, 8 months ago) |
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I would never make a puppet so obvious. Plus a puppet is hard to keep straight.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Pastywhyte]
#23177176 - 05/01/16 05:34 PM (8 years, 8 months ago) |
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=p i mean i didnt think so, but never know. was thinking it might have been for some administrative purpose or images or something
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: mupetmower]
#23177185 - 05/01/16 05:37 PM (8 years, 8 months ago) |
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Naw TC's don't need puppets for images. We get unlimited uploads
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Pastywhyte]
#23177219 - 05/01/16 05:46 PM (8 years, 8 months ago) |
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ahhhh.. nice
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: mupetmower]
#23177771 - 05/01/16 08:34 PM (8 years, 8 months ago) |
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Just shot 10 jars of Transkei up. 9 half pints, and two quarter pint containers. Had a lot of confidence doing it. Was a lot of fun. Wish I had more syringes. Getting better and better with each run, I feel, aside from the last one where I deviated from the tek.
Threw the hazmat suit and respirator on again. Hit all 10 jars in under 20 minutes. Had a small torch, and soaked alcohol paper towel on hand for each jar. Had enough left to rehit a few jars. Also saved about 1cc per pasty. I put the syringe needle cover on, and have the syringe needle point up inside a plastic bag, and put back in the shopping box, needle point up. Hopefully can do agar soon. I think I'm gonna get a PC with next paycheck. I've watched quite a few videos, and it really seems doable. I can take the whole pasty plate, and dump into sterilized grains? Agar and all? Don't gotta pull a small bit of mycelium, can throw the whole plate in?
-------------------- KSSS And PE WBS.
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Patsyblack
5% er
Registered: 05/01/16
Posts: 183
Last seen: 8 years, 7 months
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23177805 - 05/01/16 08:42 PM (8 years, 8 months ago) |
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Hazmat suit?
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AusMush



Registered: 10/24/15
Posts: 1,074
Loc: Somewhere in the Aussie s...
Last seen: 3 months, 7 days
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Patsyblack]
#23177852 - 05/01/16 08:54 PM (8 years, 8 months ago) |
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Bacterial skin flaking and fungi spores can lift off your skin and clothing easy brother
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: AusMush]
#23177890 - 05/01/16 09:02 PM (8 years, 8 months ago) |
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Better safe than sorry IMO. I don't know agar, I don't know anything aside from cakes, but the hazmat suit sprayed by the fiancé with alcohol, and respirator, has yet to fail me in terms of contams. I wish I knew more, but right now I don't. I'm trying to borrow a pressure cooker from a family member soon.
-------------------- KSSS And PE WBS.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23177946 - 05/01/16 09:18 PM (8 years, 8 months ago) |
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One day I'm going to get very good at this hobby. I graduated top 10 of my class, 3.6 GPA, I can do anything I set my mind to. I've obviously had issues and not been the best at this up to now, but one day, I'm going to have tubs upon tubs of mushrooms, and I can't wait. Thanks for the help everyone b
-------------------- KSSS And PE WBS.
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AusMush



Registered: 10/24/15
Posts: 1,074
Loc: Somewhere in the Aussie s...
Last seen: 3 months, 7 days
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23178194 - 05/01/16 10:45 PM (8 years, 8 months ago) |
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What about your tools? What about your SAB / flow hood ? How long did you take ? Was the sterilization done correctly where you using a vendor or homemade culture or from a member here there is hundreds or thousands of ways that things get contaminated.. you just gotta try and eliminate as many vectors as possible .
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