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Patsyblack
5% er
Registered: 05/01/16
Posts: 183
Last seen: 8 years, 7 months
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: mupetmower]
#23176202 - 05/01/16 12:42 PM (8 years, 8 months ago) |
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hell yeah i love patsy plates
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Patsyblack]
#23177115 - 05/01/16 05:20 PM (8 years, 8 months ago) |
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ME TOO!.. wait, is this an alt PastyWhyte account? hmmmm
anyways, i just went to the dollar store and found sooo many amazing pasty plates to use, some where 5 for a dollar and some were 10 for a dollar. one says pp5 on the bottom, so it should be alright in the PC, but the other doesnt say pp5.. so hopefully those will be ok in the PC, too, because they are amazingly perfect and the 10 for a dollar pack. they are kinda small, and clear with no obstructions on the sides, with a white cap, that opens easily, but also seals well. so excited to see if they work. gonna make some plates tonight just to see. if so, im going to go back and buy every single pack they have. and if not, im going back anyways to buy all the rest of the 5 for a dollar packs of the others which are basically glad mini knock offs, but with a clear side that doesnt have that shit on the bottom of the sides =]
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: mupetmower]
#23177160 - 05/01/16 05:30 PM (8 years, 8 months ago) |
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I would never make a puppet so obvious. Plus a puppet is hard to keep straight.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Pastywhyte]
#23177176 - 05/01/16 05:34 PM (8 years, 8 months ago) |
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=p i mean i didnt think so, but never know. was thinking it might have been for some administrative purpose or images or something
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: mupetmower]
#23177185 - 05/01/16 05:37 PM (8 years, 8 months ago) |
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Naw TC's don't need puppets for images. We get unlimited uploads
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Pastywhyte]
#23177219 - 05/01/16 05:46 PM (8 years, 8 months ago) |
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ahhhh.. nice
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: mupetmower]
#23177771 - 05/01/16 08:34 PM (8 years, 8 months ago) |
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Just shot 10 jars of Transkei up. 9 half pints, and two quarter pint containers. Had a lot of confidence doing it. Was a lot of fun. Wish I had more syringes. Getting better and better with each run, I feel, aside from the last one where I deviated from the tek.
Threw the hazmat suit and respirator on again. Hit all 10 jars in under 20 minutes. Had a small torch, and soaked alcohol paper towel on hand for each jar. Had enough left to rehit a few jars. Also saved about 1cc per pasty. I put the syringe needle cover on, and have the syringe needle point up inside a plastic bag, and put back in the shopping box, needle point up. Hopefully can do agar soon. I think I'm gonna get a PC with next paycheck. I've watched quite a few videos, and it really seems doable. I can take the whole pasty plate, and dump into sterilized grains? Agar and all? Don't gotta pull a small bit of mycelium, can throw the whole plate in?
-------------------- KSSS And PE WBS.
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Patsyblack
5% er
Registered: 05/01/16
Posts: 183
Last seen: 8 years, 7 months
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23177805 - 05/01/16 08:42 PM (8 years, 8 months ago) |
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Hazmat suit?
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AusMush



Registered: 10/24/15
Posts: 1,074
Loc: Somewhere in the Aussie s...
Last seen: 3 months, 7 days
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Patsyblack]
#23177852 - 05/01/16 08:54 PM (8 years, 8 months ago) |
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Bacterial skin flaking and fungi spores can lift off your skin and clothing easy brother
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: AusMush]
#23177890 - 05/01/16 09:02 PM (8 years, 8 months ago) |
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Better safe than sorry IMO. I don't know agar, I don't know anything aside from cakes, but the hazmat suit sprayed by the fiancé with alcohol, and respirator, has yet to fail me in terms of contams. I wish I knew more, but right now I don't. I'm trying to borrow a pressure cooker from a family member soon.
-------------------- KSSS And PE WBS.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23177946 - 05/01/16 09:18 PM (8 years, 8 months ago) |
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One day I'm going to get very good at this hobby. I graduated top 10 of my class, 3.6 GPA, I can do anything I set my mind to. I've obviously had issues and not been the best at this up to now, but one day, I'm going to have tubs upon tubs of mushrooms, and I can't wait. Thanks for the help everyone b
-------------------- KSSS And PE WBS.
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AusMush



Registered: 10/24/15
Posts: 1,074
Loc: Somewhere in the Aussie s...
Last seen: 3 months, 7 days
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Re: Innoc'd with 9mo old syringe, not seeing growth [Re: Moabfighter]
#23178194 - 05/01/16 10:45 PM (8 years, 8 months ago) |
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What about your tools? What about your SAB / flow hood ? How long did you take ? Was the sterilization done correctly where you using a vendor or homemade culture or from a member here there is hundreds or thousands of ways that things get contaminated.. you just gotta try and eliminate as many vectors as possible .
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