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Invisibletoad857
President of theUnited States

Registered: 02/02/04
Posts: 283
flame sterilization of syringe
    #2317270 - 02/09/04 11:52 AM (14 years, 4 months ago)

do not have: alcohol lamp or 'wind proof' lighter

do have: regular gas station lighter


i know that using the yellow flame will 'burn' the syringe (it will leave carbon residue all over it)..
but what about using the tiny blue part of the flame to sterilize? i assume it is the same principle as using an alcohol lamp's blue flame, right? thanks!


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OfflineOmegaRed
Myco-Floydianenthusiast

Registered: 04/16/03
Posts: 193
Loc: right behind you
Last seen: 14 years, 3 months
Re: flame sterilization of syringe [Re: toad857]
    #2317314 - 02/09/04 12:17 PM (14 years, 4 months ago)

I just use a standard Bic lighter, and then wipe off the carbon residue with some alcohol before innoculating. Never had any problem with it yet. Make sure your syringe is completely cool before pushing any spores through the needle. It would be a shame to do all that work and then kill all your spores before they get anywhere near your substrate.


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I'm not sleepy and there's no place I'm going to.
Hey Mr. Tamborine Man play a song for me,
In the jingle jangle morning I'll come following you.


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OfflineDuBri
Stranger

Registered: 04/21/04
Posts: 24
Last seen: 4 years, 11 months
Re: flame sterilization of syringe [Re: toad857]
    #2317349 - 02/09/04 12:27 PM (14 years, 4 months ago)

Just squirt a little extra solution through, the first through might die but theres a thousand more behind them. Soon as any solution gets through the needle its pretty much cool. Also, I had contam problems wipping down the needle with rubbing alcohol after flame sterelizing it. Now I rub it with alcohol, hit it with a flame and it gets hot fast. The carbon residue has never hurt a thing.


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Offlinetrancefishy
ambiguity

Registered: 07/24/02
Posts: 365
Loc: under the stars
Last seen: 10 years, 27 days
Re: flame sterilization of syringe [Re: toad857]
    #2318353 - 02/09/04 05:18 PM (14 years, 4 months ago)

why not just splurge the 73 cents for a bottle of rubbing alcohol? i dump some in the bottom of a shot glass (that I use for espresso shots, inverted, of course) and light it on fire, you dont need anything special. off the top of my head, put alcohol in a thimble, a little bowl made of aluminum foil, a small teacup, a soup spoon. those aren't all the best ideas, but what i'm saying is, alcohol burns fine in puddle form.


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Anonymous

Re: flame sterilization of syringe [Re: toad857]
    #2318460 - 02/09/04 05:58 PM (14 years, 4 months ago)

a standard butane cigarette lighter is just fine. do not hold the needle in the flame, but just above it. it will get plenty hot this way and a carbon residue will not be left.


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Anonymous

Re: flame sterilization of syringe [Re: toad857]
    #2318561 - 02/09/04 06:25 PM (14 years, 4 months ago)

you don't even need a flame...wiping down your syringe with rubbing alcohol is all you need


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Offlinevermonster
Lost Sailor

Registered: 10/17/03
Posts: 207
Loc: Shangri La
Last seen: 4 years, 8 months
Re: flame sterilization of syringe [Re: toad857]
    #2318713 - 02/09/04 07:11 PM (14 years, 4 months ago)

Afoaf just sets the syringe (needle down) in a small jar with
1" of alcohol in it between jars.


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