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OfflineLosAndreas36
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Registered: 04/05/15
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Bacteria again?
    #23172605 - 04/30/16 12:28 PM (8 years, 8 months ago)

Hi all
Check my jars. My temp is not stable, from 25.5 to 27. I see some wet grains, its bacteria? why, if its sterile water after PC. I soaked grains for 12 hrs in h2o2. I PCed jars 2 times, more than 2 hours each. I used UV lamp to make spore suspension, i did everything sterile, whats wrong? This is like my 4-5th trying, i can't have jars without contaminations. I tried with and without antibiotics.

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OfflineLosAndreas36
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Re: Bacteria again? [Re: LosAndreas36]
    #23172607 - 04/30/16 12:29 PM (8 years, 8 months ago)

also i used more than 4 spoons of vermiculite, and let grains dry

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OfflineLosAndreas36
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Re: Bacteria again? [Re: LosAndreas36]
    #23176352 - 05/01/16 01:31 PM (8 years, 8 months ago)

Anyone? should i throw jars? i see a few wet grains, but myc is eating substrate, so i think its about like 4-5 days to fully colonize. if cut out some uncolonized spots, and put in bulk, harvest some fruits and boil fruits in tea. it will kill bacteria as i heard. it's  because of not stable temp this time of year. any opinions guys?


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Invisibleazur
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Re: Bacteria again? [Re: LosAndreas36]
    #23183252 - 05/03/16 08:05 AM (8 years, 8 months ago)

Take better pics. And hold your phone sideways so the pics appear upright.
Why did you add vermiculite?


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OfflineStill_tripping
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Re: Bacteria again? [Re: LosAndreas36]
    #23183463 - 05/03/16 09:19 AM (8 years, 8 months ago)

Quote:

LosAndreas36 said:
Anyone? should i throw jars? i see a few wet grains, but myc is eating substrate, so i think its about like 4-5 days to fully colonize. if cut out some uncolonized spots, and put in bulk, harvest some fruits and boil fruits in tea. it will kill bacteria as i heard. it's  because of not stable temp this time of year. any opinions guys?




It dosen't sound as though you are following any tech that I have read. You are not telling us about any of your procedures, you are not even showing us your lids so it is hard to advise you what may be wrong. All your efforts of over PC'g and presoaking grains in H2O2 are all directed toward ensuring your grains are sterile but we've no information to help determine if maybe you are introducing the contams in your knoc'g up procedures. Without these details only thing I could suggest is to go back and follow a tech to the letter and you should have success.

As to whether you will get fruits or not from these jars after spawning to bulk that will depend on if it is contam'd and how quickly those contams will grow versus the fruits. I wouldn't eat contaminated shrooms if I were you and I wouldn't count on tea protecting you much from contams.


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OfflineLosAndreas36
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Re: Bacteria again? [Re: Still_tripping]
    #23185033 - 05/03/16 03:43 PM (8 years, 8 months ago)

Tell me, if its contaminated. i'll show better pics tommorow. WEll my temp is not stable, my jar lids are just inoculation port with RTV. ask me what you want to know. i used UV lamp before starting anything, i washed everything in alcohol etc. Burned needle, and such sterile thing, Used gloves and mask. I see just a few grains like that. People telling me its because of not stable temp, i can have stable room 23-24 but its not enough, but i cant have always 28, only like 25-28, so idk


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OfflineLosAndreas36
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Registered: 04/05/15
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Re: Bacteria again? [Re: LosAndreas36]
    #23185035 - 05/03/16 03:43 PM (8 years, 8 months ago)

also no smell, just fine grain smell when i open container with jars


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OfflineStill_tripping
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Re: Bacteria again? [Re: LosAndreas36]
    #23191779 - 05/05/16 10:29 AM (8 years, 8 months ago)

Quote:

LosAndreas36 said:
Tell me, if its contaminated. i'll show better pics tommorow. WEll my temp is not stable, my jar lids are just inoculation port with RTV. ask me what you want to know. i used UV lamp before starting anything, i washed everything in alcohol etc. Burned needle, and such sterile thing, Used gloves and mask. I see just a few grains like that. People telling me its because of not stable temp, i can have stable room 23-24 but its not enough, but i cant have always 28, only like 25-28, so idk




I doubt it is a temp problem. Higher or lower temps will slow myc growth, even stop it if the temp is far enough off but I don't see that with the bit of variation you state. I'll incubate/colonize around 78 F to 82 F, and fruit about 78 F to 81 F. These are simply room temps and plus an AC set at low in the fruiting room. The temps vary all the time. You can safely stop fixating on your temps, that is not your contam problem.

You say you've only got a noc port on your lids, that is a problem. Your myc needs gas exchange to grow. It needs to exchange CO2 which it produces with O which it needs to grow. Too high CO2 levels will also slow or stop the myc from growing. When myc slows or stops it can allow contams to get the upper hand, you want the myc to grow aggressively as it will help control the growth of contams. Your lids need some sort of GE filter in them.

You shouldn't "wash" things in alcohol. You need to wash them with detergent, then before use (if you haven't sterilized them) you can rinse them in alcohol. Firstly you don't want to try and sterilize dirt and secondly alcohol will kill bacteria but it does little against spores.

You say you flame sterilized the needle. Did you hold it up or down when you squeezed a drop or so through to cool it? If you allow those drops to run the length of the needle to the syringe and then back again to the tip as you go to noc your jar then those drops can carry contams right back to the needle tip just as you are poking it into the jar.

You don't mention the use of a SAB in a still air room. I've tried to do without but with little success so to me these have become a necessity. It is a case of thinking about each and every procedure you perform and I find it useful to think of contams like dust that is floating everywhere and coating everything. The slightest movement or contyact can send that dust flying or sticking to anything and everything. Now how are you going to get those spores/myc into your grain jars without any of that dust. Think about it in those terms and you can usually identify yourself where your procedures can be improved.

At this stage you are not even sure those jars are contam'd. You say you've sniffed them so that probably means you opened them, a big no no. Think about that dust floating everywhere around you and you can see why. Keep a watch on them because if it is contams it will become painfully obvious quite shortly. If so throw them out and start again.


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