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OfflineGrambo
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open air innoc
    #23170803 - 04/29/16 09:16 PM (8 years, 8 months ago)

I didn't have the equipment at the time so I did an open air inoculation. I did it on the kitchen stove straight from the cooker, removed and replaced the foil quickly, with a tub lid sprayed with Lysol to carry them to the spare room. I heated the syringe and shot the spores through an antiseptic wipe, and covered my face as to not breathe on them.

Just wondering if anyone else has had much success.

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InvisibleMad Season
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Re: open air innoc [Re: Grambo]
    #23170804 - 04/29/16 09:18 PM (8 years, 8 months ago)

You did it straight from the cooker? What does that mean?

Also is this pf tek? Does it have a verm barrier or something?


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OfflineGrambo
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Re: open air innoc [Re: Mad Season]
    #23170817 - 04/29/16 09:21 PM (8 years, 8 months ago)

Yes, PF tek. sorry.

Next day when it was cool, straight out of the cooker onto the tray and inoculate one at a time in 4 holes and covered again.

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InvisibleMad Season
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Re: open air innoc [Re: Grambo]
    #23170822 - 04/29/16 09:24 PM (8 years, 8 months ago)

Probably good. Ideally you do it in a sab. Pf tek has been done in open air for the longest time with success though.


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OfflineHierophant
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Re: open air innoc [Re: Mad Season]
    #23170847 - 04/29/16 09:32 PM (8 years, 8 months ago)

Maybe I'm not understanding something but if you just had foil over the jars you didn't need to remove it, inoculate and recover, you could have just penetrated through the foil with the syringe. You have a verm barrier correct?


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InvisibleMad Season
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Re: open air innoc [Re: Hierophant]
    #23170865 - 04/29/16 09:35 PM (8 years, 8 months ago)

Or just don't have any foil after inoc lol


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Edited by Mad Season (04/29/16 09:35 PM)

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OfflineGrambo
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Re: open air innoc [Re: Mad Season]
    #23170869 - 04/29/16 09:36 PM (8 years, 8 months ago)

That's what I had read so I thought I'd give it a try. I get the idea of bacterial transportation and understand most concepts but there are some situations that you can cut corners IF you cut very carefully.

I will start experimenting with WBS on a bulk system next. I just really want to understand what I am doing and why as I go so I don't end up with a Todd-Toght.

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OfflineGrambo
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Re: open air innoc [Re: Grambo]
    #23170885 - 04/29/16 09:41 PM (8 years, 8 months ago)

Verm barrier, correct. I read so many reviews on whether to cover them again or not I just did. If the only gas exchange is the release of carbon dioxide then the foil barrier is helping to keep airbourne particulates from landing directly on the lids and not hindering the release of co2. The mycelium needs no oxygen at this point so there is no harm in keeping the foil on the jars in my opinion.

I felt I was taking enough chances as is so I opted to go the route I did.

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OfflineHierophant
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Re: open air innoc [Re: Mad Season]
    #23170889 - 04/29/16 09:41 PM (8 years, 8 months ago)

Quote:

Mad Season said:
Or just don't have any foil after inoc lol




He didn't specify whether or not he used the canning lids so I assumed the foil was his lid cause otherwise, yeah, not necessary after the cook


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OfflineHierophant
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Re: open air innoc [Re: Grambo]
    #23170899 - 04/29/16 09:45 PM (8 years, 8 months ago)

Quote:

Grambo said:
Verm barrier, correct. I read so many reviews on whether to cover them again or not I just did. If the only gas exchange is the release of carbon dioxide then the foil barrier is helping to keep airbourne particulates from landing directly on the lids and not hindering the release of co2. The mycelium needs no oxygen at this point so there is no harm in keeping the foil on the jars in my opinion.

I felt I was taking enough chances as is so I opted to go the route I did.




Oh ok, I thought the foil was your lid. It really isn't necessary after the cook. The verm barrier at the top will prevent any competing spores from getting to the nutrients inside. If it makes you more comfortable than by all means but the mycelium does need to breath and the inoculation points allow for gas exchange so it's better to leave them uncovered


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There is a golden book kept in my heart and guarded by my soul, written in Divine Light and bound with the veins of the Earth.

Edited by Hierophant (04/29/16 09:47 PM)

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InvisibleMad Season
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Re: open air innoc [Re: Hierophant]
    #23170918 - 04/29/16 09:50 PM (8 years, 8 months ago)

It takes almost air tight for GE to be restricted. I find what makes foil bad is it isn't porous, and the heat from colonization would cause condensation, and pooling on the lid under the foik, which when it drips down the inoc holes it ruins the integrity of the verm barrier.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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