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Offlinecandela
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Registered: 09/04/15
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Last seen: 6 months, 30 days
Mr Green Mold kicking my ass!!! Help!!!
    #23169650 - 04/29/16 03:43 PM (8 years, 8 months ago)

I have been sucessful in the past, but as of recently, my totes keep getting green contaminates 80% of the time when I put it into FC.

WTF can be the prob?

I threw away my pollyfill and I am now trying just micropore.

20% of my totes go well, so I dont think is an issue with my sterilization, or pasteurization methods.

Advice of WTF might be going on?

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Mr Green Mold kicking my ass!!! Help!!! [Re: candela]
    #23169688 - 04/29/16 03:54 PM (8 years, 8 months ago)

My guess is your spawn is contaminated or your substrate isn't properly prepared. What kind of substrate are you using and how are you prepping it? If coir, what brand?

Are you cleaning the totes pretty damn well after they're having subs contaminated in them? Maybe it's also time to give the house a good cleaning if you've been having such bad contamination issues in your homes. Subs repeatedly sporulating in your grow area is not a good thing.

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OfflineBigbadwooof
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Re: Mr Green Mold kicking my ass!!! Help!!! [Re: TheEaglesGift]
    #23169774 - 04/29/16 04:20 PM (8 years, 8 months ago)

Are you using antibiotic agar? I think that has been the source of my problems, and my success rate has been about the same as yours for a bit now.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

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Offlinecandela
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Registered: 09/04/15
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Re: Mr Green Mold kicking my ass!!! Help!!! [Re: Bigbadwooof]
    #23169931 - 04/29/16 05:07 PM (8 years, 8 months ago)

Before I transfer any grains, I take a whiff (smell) of them and they smell great, just like mushies.

I do pasteurize my substrate (ECO Earth coir and verm) by pouring boiling water onto it, inside of a bucket.

I never had a prob using this tek, and I do clorox the shit, and soap the shit out of any contaminated areas or buckets.

For some reason I am thinking that the pollyfill was the issue!! 

Can anybody confirm whether pollyfill is sterile from the bag.  I just cant fanthom that pollyfill is manafactured in a clean enviroment.  I picture pollyfill being bagged in a chinese, dirty alley way! (No pun intended)

Microtape is designed for sterile applications, hence my thought that microtape might be the better choice!

I just read on another post, that when fruiting in temperatures over 81° degrees is ideal for contams to germinate.  I know for a fact that my fruiting temperatures are in the 85°-90° degree area, so I am now thinking that this might be the issue, but it also doesnt explain why some of my totes have grown ok in these temperatures?

I dont really know?????

Any other thoughts are appreciated

Edited by candela (04/29/16 05:27 PM)

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OfflineHowToGrow
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Re: Mr Green Mold kicking my ass!!! Help!!! [Re: candela]
    #23170105 - 04/29/16 06:15 PM (8 years, 8 months ago)

Yep sounds like you could have a few issues going on.

One, I would suggest properly pasteurizing your substrate.

Two, be sure to let your sub cooldown to room temp (70-80degrees) before mixing your sub and grain.

Three, you need your room temps to also be in the 70-80s range. I find the best temps for my growing conditions are 74 during the day and 72 at night..anything over and you are baking your bulk subs. Remember they produce heat as well while colonizing and fruiting. If your outside temps are 85 I'm betting your tubs are pushing 90+

Clorox may be a bit much lol. I simply wash out all my tubs with hot water and soap and before I prep them for bulk spawning I wipe them down with 70% alcohol. Also I'd suggest wearing gloves while preping bulk. I usually switch to a clean pair of gloves for every tub just in case.

Polyfil isn't the issue I promise. I've used walmart polyfil for years with no issues besides it being messy as hell. Though I do like micropore tape better because it's easier to handle and you don't have to pick it from the fruits. (Currently logging differences between micropore tape vs polyfil)

Good luck!


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OfflineBigbadwooof
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Re: Mr Green Mold kicking my ass!!! Help!!! [Re: candela]
    #23170301 - 04/29/16 07:15 PM (8 years, 8 months ago)

Quote:

candela said:
Before I transfer any grains, I take a whiff (smell) of them and they smell great, just like mushies.

I do pasteurize my substrate (ECO Earth coir and verm) by pouring boiling water onto it, inside of a bucket.

I never had a prob using this tek, and I do clorox the shit, and soap the shit out of any contaminated areas or buckets.

For some reason I am thinking that the pollyfill was the issue!! 

Can anybody confirm whether pollyfill is sterile from the bag.  I just cant fanthom that pollyfill is manafactured in a clean enviroment.  I picture pollyfill being bagged in a chinese, dirty alley way! (No pun intended)

Microtape is designed for sterile applications, hence my thought that microtape might be the better choice!

I just read on another post, that when fruiting in temperatures over 81° degrees is ideal for contams to germinate.  I know for a fact that my fruiting temperatures are in the 85°-90° degree area, so I am now thinking that this might be the issue, but it also doesnt explain why some of my totes have grown ok in these temperatures?

I dont really know?????

Any other thoughts are appreciated




When you say 'I smell my grains before transfer', I hope you mean either:

A) Through the filter on the lid (My sense of smell isn't good enough for this)

or

B) Before you spawn. Transfer generally refers to Grain to Grain transfer, or agar plate transfers. You normally sniff before spawning.

Now, micropore tap  over your monotub holes will only make all of your problems worse. The polyfill isn't used to filter anything. At this stage, if your spawn is good, and your pasteurization is up to par, you shouldn't have to worry about sterility (for the most part... Don't drop food crumbs into the tub or anything like that lol). The polyfill is only there to regulate airflow and humidity.

Trich thrives in high CO2, low FAE, stagnate/still air. Mushroom mycelium thrives under specific FAE conditions, depending on the species. At this stage, all mushroom species require some amount of FAE, and micropore tape will not permit enough.

Stick with the tried and true methods of doing things, buddy :wink:

Edit: Also, the heat certainly causes problems, but it's not the end of the world. Mold likes heat, and so does bacteria. Heat causes them to metabolize and multiply faster. ~70-75F is where you want your fruiting room to be. I have certainly grown in higher temperatures though. You may benefit from growing a strain that comes from an equatorial climate, I am not sure.

I think you are probably having hidden bacterial problems in your jars. I would suggest starting spores on antibiotic agar, and then doing a couple transfers onto normal plates. If you are unfamiliar with agar work, I can give you some pointers. Look up 'pasty plates' first though.

I can almost promise you that your problems stem from sterile technique when making jars. If you are using coir, it is highly unlikely that it is your pasteurization technique. I would highly suggest focusing on sterility when making jars, and agar work. Don't waste time fidgeting with other shit.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

Edited by Bigbadwooof (04/29/16 07:26 PM)

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Mr Green Mold kicking my ass!!! Help!!! [Re: Bigbadwooof]
    #23171237 - 04/29/16 11:19 PM (8 years, 8 months ago)

Coir doesn't need to be properly pasteurized.

I'd bet large amounts of cash that your poly isn't the issue either.

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Mr Green Mold kicking my ass!!! Help!!! [Re: TheEaglesGift]
    #23171248 - 04/29/16 11:22 PM (8 years, 8 months ago)

Quote:

I would highly suggest focusing on sterility when making jars, and agar work. Don't waste time fidgeting with other shit.





This, absolutely.

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Offlinefatboyjeff
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Registered: 02/11/16
Posts: 60
Last seen: 1 year, 4 months
Re: Mr Green Mold kicking my ass!!! Help!!! [Re: TheEaglesGift]
    #23171380 - 04/30/16 12:10 AM (8 years, 8 months ago)

how are you knocking your jars up? ms or agar?

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Offlinetump
ban the undead
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Registered: 03/17/16
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Re: Mr Green Mold kicking my ass!!! Help!!! [Re: fatboyjeff]
    #23171684 - 04/30/16 04:41 AM (8 years, 8 months ago)

I know what the problem is. Good news you can fix it. Bad news its hard to do. You can to competely clean the area with bleach and lower the temp. Poly fill isnt the issue. Coir contams 80% in a area that means with there is mean green growing its own pacth somewhere close to your grow space within 20 feet. I had the same problem for a while where no matter what i did i alway contams green. After the 10th tub i cleaned the shit out of the house and fould a green spore pacths on bottom of my dresser and two of my bed post. Trish is everywhere. For the time being move where you spawn yo the coir and grow for a little while and when you do spawn spray diffecten. Until it hurts to breath, rest one min then do your tub.

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Mr Green Mold kicking my ass!!! Help!!! [Re: tump]
    #23172953 - 04/30/16 02:25 PM (8 years, 8 months ago)

Quote:

tump said:
I know what the problem is. Good news you can fix it. Bad news its hard to do. You can to competely clean the area with bleach and lower the temp. Poly fill isnt the issue. Coir contams 80% in a area that means with there is mean green growing its own pacth somewhere close to your grow space within 20 feet. I had the same problem for a while where no matter what i did i alway contams green. After the 10th tub i cleaned the shit out of the house and fould a green spore pacths on bottom of my dresser and two of my bed post. Trish is everywhere. For the time being move where you spawn yo the coir and grow for a little while and when you do spawn spray diffecten. Until it hurts to breath, rest one min then do your tub.




This is a pretty big assumption.

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OfflineBigbadwooof
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Re: Mr Green Mold kicking my ass!!! Help!!! [Re: TheEaglesGift]
    #23173777 - 04/30/16 07:11 PM (8 years, 8 months ago)

I've had a 100% moldy tub sitting in my grow room with colonizing tubs and not had a problem with any of the other tubs. I don't think that's the issue. I think it's probably a bacterial problem, because they can be difficult to spot until you get an eye for it. You can use antibiotics to clean up a culture, but don't use them continuously. That has caused me some nightmarish problems.


--------------------
"It is no measure of good health to be well adjusted to a profoundly sick society," - Jiddu Krishnamurti
FARTS
"No my dog won't bite you, tho it had the right to. You ought to give him credit, cuz he knows I woulda let him" - Modest Mouse (And Wooof!)
Every one of you should see this video.
"Facts are chiels that winna ding, and downa be disputed" - Robert Burns

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,972
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Re: Mr Green Mold kicking my ass!!! Help!!! [Re: Bigbadwooof]
    #23173787 - 04/30/16 07:16 PM (8 years, 8 months ago)

Quote:

Bigbadwooof said:
I've had a 100% moldy tub sitting in my grow room with colonizing tubs and not had a problem with any of the other tubs. I don't think that's the issue. I think it's probably a bacterial problem, because they can be difficult to spot until you get an eye for it. You can use antibiotics to clean up a culture, but don't use them continuously. That has caused me some nightmarish problems.




:manofapproval:

People see mold and never seem to connect bacteria as a vector.

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