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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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can niger mold live through a pc session??
#23163439 - 04/27/16 10:55 PM (8 years, 8 months ago) |
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Just wondering because I've been reading about it and nothing I've read says yay or nay ... so if my whole oats had the spores on it then I PC would it kill it or not ? Or how would I find out??
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 7 years, 9 days
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Re: can niger mold live through a pc session?? [Re: Boogieman47]
#23163459 - 04/27/16 11:03 PM (8 years, 8 months ago) |
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I couldn't tell you the answer to your first question. However, I believe to answer your second question if you were to experiment by PCing a jar infected with the mold, and then transfer a grain from the jar to some agar and wait and see if anything grows. That would allow you to see if the mold survived.
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Highwalker
Edited by lukehighwalker710 (04/27/16 11:04 PM)
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Boogieman47
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Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Very true I guess I'll do that and repost the results I didn't even think of that
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lukehighwalker710
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Registered: 03/04/16
Posts: 609
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Last seen: 7 years, 9 days
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Re: can niger mold live through a pc session?? [Re: Boogieman47]
#23164342 - 04/28/16 08:32 AM (8 years, 8 months ago) |
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yep! I'll watch for the results!
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Highwalker
Edited by lukehighwalker710 (04/28/16 08:33 AM)
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Mold dies easily in the PC. Bacteria, due to their endospores, are why we have to sterilize for an hour plus. I sterilize all my spawn for 2+ hours at 15 psi to be safe.
To answer your question, all mold will be long dead by the time you make it to an hour at 15 psi.
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Boogieman47
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Registered: 03/05/16
Posts: 9,712
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Re: can niger mold live through a pc session?? [Re: TheEaglesGift]
#23165543 - 04/28/16 03:03 PM (8 years, 8 months ago) |
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I've been getting it in almost every jar since I changed to whole oats I just don't see how I Fuck it up so much I just finished my flow hood so hopefully it was just my SAB
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: can niger mold live through a pc session?? [Re: Boogieman47]
#23167117 - 04/28/16 10:01 PM (8 years, 8 months ago) |
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Quote:
noob47 said: I've been getting it in almost every jar since I changed to whole oats I just don't see how I Fuck it up so much I just finished my flow hood so hopefully it was just my SAB
How long are you sterilizing? What psi?
Has anything else changed besides the oats? It's very easy to attribute failure to one change in procedure, but if you dig a little deeper you'll see that other inconsistencies in your technique may be at fault and maybe not the original vector(oats, in your case). Just a thought.
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Boogieman47
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Registered: 03/05/16
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Re: can niger mold live through a pc session?? [Re: TheEaglesGift]
#23167125 - 04/28/16 10:05 PM (8 years, 8 months ago) |
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I PC at 15 for two hours but my sab is just a cardboard box with saran wrap on top but I spray the Fuck out of it with Lysol and wipe down with 91% I am going to get some 70 or just dilute the 91 ... but I do everything the same other than the first few jars were g2g I was given from a friend and the rest have been agar I've made
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: can niger mold live through a pc session?? [Re: Boogieman47]
#23167259 - 04/28/16 10:55 PM (8 years, 8 months ago) |
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If you're pcing for 2 hours at 15 psi, then the mold is getting in from your inoculation or ge filters/lids. Molds are wiped the fuck out at those temps/times, so I can safely say it's not your oats.
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Boogieman47
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Registered: 03/05/16
Posts: 9,712
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Re: can niger mold live through a pc session?? [Re: TheEaglesGift]
#23167366 - 04/28/16 11:28 PM (8 years, 8 months ago) |
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Ya I guess I've been trying to find a reason to blame other then myself haha I'm using my flow hood tonight and I should see some good growth hopefully
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: can niger mold live through a pc session?? [Re: Boogieman47]
#23167417 - 04/28/16 11:45 PM (8 years, 8 months ago) |
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A flow is still only going to be as good as your technique. If you have shitty technique in a still air box, you're still going to have shitty technique in front of the flow hood.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: can niger mold live through a pc session?? [Re: TheEaglesGift]
#23167418 - 04/28/16 11:46 PM (8 years, 8 months ago) |
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What are you using for lids/ge filters?
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Boogieman47
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Registered: 03/05/16
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Re: can niger mold live through a pc session?? [Re: TheEaglesGift]
#23167420 - 04/28/16 11:49 PM (8 years, 8 months ago) |
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Ez felt and poly FIL which both jars have contams
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: can niger mold live through a pc session?? [Re: Boogieman47]
#23167575 - 04/29/16 12:53 AM (8 years, 8 months ago) |
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Ditch the ez felt, I've never trusted it despite some respectable growers here having good success with it. Make sure you're stuffing your poly pretty damn tight.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: can niger mold live through a pc session?? [Re: TheEaglesGift]
#23167659 - 04/29/16 01:36 AM (8 years, 8 months ago) |
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OK I'll ditch the ez felt and I think the poly is packed in there pretty good I appreciate the responses I just finished doing agar transfers and a MS inoculation with my flow hood first time with that ... let's hope it went well I am pretty clumsy bumping shit around but I'm hoping for the best
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: can niger mold live through a pc session?? [Re: Boogieman47]
#23167691 - 04/29/16 02:01 AM (8 years, 8 months ago) |
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Practice makes perfect. Steady but quick and controlled hand movements are key. Always make sure your hands are never between the hood and the opened sterile media containers(your petri dishes or your spawn jars/bags) or the laminar flow will blow shit from your hands into your containers.
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