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OfflineShroomboy5
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Easy way to inoculate for a lazy, low confidence guy?
    #23165394 - 04/28/16 02:27 PM (8 years, 8 months ago)

So when my fruits were ready, I used the same knife/scissors that I only really wiped with alcohol initially, to cut off the caps and placed them on one sheet of alcohol wiped foil. I just quickly cut off the stems and placed the caps onto the foil and placed another sheet of foil to cover them over-night. The next day I removed the caps and they left prints so I just quickly cut out around the prints and folded them sealed and stored them.

Can someone give me a simple half-assed way to inoculate with one of these prints? I don't know where to get syringes either, I just need the easiest way possible to mix some of these spore with water and inoculate them.

Any comments?

Edited by Shroomboy5 (04/28/16 02:30 PM)

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Shroomboy5]
    #23165431 - 04/28/16 02:35 PM (8 years, 8 months ago)

You get out what you put in... Half ass doesn't work for this :shrug:


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Shroomboy5]
    #23165432 - 04/28/16 02:36 PM (8 years, 8 months ago)

The best you can do is learn agar asap, so that you can make the most out of that print. This way you won't have to make syringes anymore because it is a poor way to inoculate your projects, since you never know if the syringe might be contaminated.

The basics, check out these links in order, you won't look back. Don't have a PC? No problem, you can steam-sterilize agar for 2 hours.

https://www.shroomery.org/forums/showflat.php/Number/19208976
https://www.shroomery.org/forums/showflat.php/Number/21032261#21032261
https://www.shroomery.org/forums/showflat.php/Number/22833314#22833314

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Shroomboy5]
    #23165433 - 04/28/16 02:37 PM (8 years, 8 months ago)

Your process sounded less than sterile. If imagine if you took those to water and made a syringe that would be bunk.

If you had agar you could use that print. But it doesn't seem like you are really willing to put in the effort to learn correct methods. Just buy another syringe from a vendor :shrug:


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: SloppyJoseph]
    #23165444 - 04/28/16 02:39 PM (8 years, 8 months ago)

Quote:

Josex said:
The best you can do is learn agar asap, so that you can make the most out of that print. This way you won't have to make syringes anymore because it is a poor way to inoculate your projects, since you never know if the syringe might be contaminated.

The basics, check out these links in order, you won't look back. Don't have a PC? No problem, you can steam-sterilize agar for 2 hours.

https://www.shroomery.org/forums/showflat.php/Number/19208976
https://www.shroomery.org/forums/showflat.php/Number/21032261#21032261
https://www.shroomery.org/forums/showflat.php/Number/22833314#22833314



This.

Quote:

SloppyJoseph said:
Your process sounded less than sterile. If imagine if you took those to water and made a syringe that would be bunk.

If you had agar you could use that print. But it doesn't seem like you are really willing to put in the effort to learn correct methods. Just buy another syringe from a vendor :shrug:



and this.


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OfflineShroomboy5
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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: SloppyJoseph]
    #23165461 - 04/28/16 02:43 PM (8 years, 8 months ago)

Quote:

Josex said:
The best you can do is learn agar asap, so that you can make the most out of that print. This way you won't have to make syringes anymore because it is a poor way to inoculate your projects, since you never know if the syringe might be contaminated.

The basics, check out these links in order, you won't look back. Don't have a PC? No problem, you can steam-sterilize agar for 2 hours.

https://www.shroomery.org/forums/showflat.php/Number/19208976
https://www.shroomery.org/forums/showflat.php/Number/21032261#21032261
https://www.shroomery.org/forums/showflat.php/Number/22833314#22833314




Quote:

SloppyJoseph said:
Your process sounded less than sterile. If imagine if you took those to water and made a syringe that would be bunk.

If you had agar you could use that print. But it doesn't seem like you are really willing to put in the effort to learn correct methods. Just buy another syringe from a vendor :shrug:




Okay, I'll read the links Josex posted. You're saying I can use the prints I made with the agar method? Because I might have a second flush coming for new caps..I definitely hate the idea of making a syringe and I'm afraid to order online again

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Shroomboy5]
    #23165516 - 04/28/16 02:56 PM (8 years, 8 months ago)

I would suggest buying premixed agar powder from a shroomery sponsor & a few sleeves of plastic petris. You mix the agar powder with water, pour it into a liquor bottle & sterilize 40 min @ 15psi. Allow to cool until it doesn't burn your hand then pour into the petris. About 500ml of agar will make ~20 petri dishes. Once the plates solidify you can store the ones you don't need to use immediately in ziploc bags. Make sure to drill a hole in the cap of the bottle & place a filter (polyfill or sythetic disc).



Agar may sound intimidating but it is an essential skill if you want to grow mushrooms, and it's not too bad after you do it a couple times. Most of us find petri work to be the most enjoyable part of cultivation!

If you can make your plates before your next flush, you can take a piece of tissue from a fresh mushroom and put it on a plate. That will make a clone culture that will produce nicely.

All of these are clones:



RogerRabbit has some nice videos on all of this stuff at http://mushroomvideos.com/, and I believe you can buy them for around $10. They are worth every penny!


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: PirateSwazey]
    #23165540 - 04/28/16 03:02 PM (8 years, 8 months ago)

Hmm even if I did do this do you even think I'd be able to find and get the agar powder from a sponsor in time?

I'm gonna try to learn more about the agar so it seems less intimidating

I was also thinking of just paying ~20 dollars for a single syringe online when I need spores but I'm still afraid that they are not stealthy there's a risk to doing that right?

And my final thought is to just try to find a right sized syringe and just go with that step properly on my next flush...

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Shroomboy5] * 1
    #23165552 - 04/28/16 03:06 PM (8 years, 8 months ago)

The thing about using spores to inoculate is they were collected from a mushroom that fruited in open air, in a mushroom growers house, who undoubtedly has tons of green mold spores floating around. The mold spores often find their way onto the spore print when they are collected, then put into a syringe by a spore vendor. It is not necessarily anybody's fault that this happens, it's just the nature of the beast. That's why we use agar, because you can ensure you have a clean culture that you are inoculating with.

If you learn agar, you will never need to worry about needles again :smile:


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: PirateSwazey]
    #23165575 - 04/28/16 03:14 PM (8 years, 8 months ago)

Once you make a print (the correct and sterile way) you can store it for a decade. If you are interested in agar, make a new set of prints from your second flush fruits (the ones you made are undoubtably contaminated) and then store it correctly and when you are ready with the agar the print will be good to go.


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: PirateSwazey]
    #23165583 - 04/28/16 03:16 PM (8 years, 8 months ago)

I can do agar without a pressure cooker?


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OfflineSloppyJoseph
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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Moabfighter]
    #23165588 - 04/28/16 03:18 PM (8 years, 8 months ago)

Apparently :shrug:

I wouldn't. But I haven't tried it so I can't vouch or deny.


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Moabfighter]
    #23165601 - 04/28/16 03:23 PM (8 years, 8 months ago)

Quote:

Moabfighter said:
I can do agar without a pressure cooker?



Yes, I can vouch for it :smile:

Steam-sterilize it for 2 hours. I'm pretty sure 2 hours is overkill, but better safe than sorry.

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Josex]
    #23165619 - 04/28/16 03:30 PM (8 years, 8 months ago)

I forgot to mention that if you want to do some bulk bottles you can also do them without a PC.

You can steam-sterilize these beauties for three hours. And it isn't more difficult than doing cakes. You can steam-sterilize them for 3 or 4 hours but again I think this is overkill, you should probably be ok with 2,5 to 3 hours sterilization.

Edited by Josex (04/28/16 03:36 PM)

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OfflineShroomboy5
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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Josex]
    #23165635 - 04/28/16 03:35 PM (8 years, 8 months ago)

Alright man. Is the Agar powder safe to ship to CA? Looks like that's 30-40 dollars. And do you know where I can get the petris?

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Shroomboy5]
    #23165657 - 04/28/16 03:44 PM (8 years, 8 months ago)

Quote:

Shroomboy5 said:
Alright man. Is the Agar powder safe to ship to CA? Looks like that's 30-40 dollars. And do you know where I can get the petris?



Don't do pretris, there are more steps to it where you can fuck up. You'll be better off reading the links I posted. It is a no-pour agar tek and as easy as prepping jello.

I live in Spain and I buy my agar in a health food store, but as far as I know you can also find agar in health food stores in the States. Some people buy them in Assian food stores (in the States). Check those places first and if you can't find it you could always resort to the internet.

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: PirateSwazey]
    #23165722 - 04/28/16 04:03 PM (8 years, 8 months ago)

Quote:

PirateSwazey said:
You get out what you put in... Half ass doesn't work for this :shrug:



1st step stop being lazy, putting yourself down and believe in yourself!
:fuckyeahdance:
YOU CAN DO IT, its not too hard.


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: MysticMoteToter]
    #23165765 - 04/28/16 04:11 PM (8 years, 8 months ago)

Quote:

MysticMoteToter said:
Quote:

PirateSwazey said:
You get out what you put in... Half ass doesn't work for this :shrug:



1st step stop being lazy, putting yourself down and believe in yourself!
:fuckyeahdance:
YOU CAN DO IT, its not too hard.



:awelol: True that! But I agree with OP that making a spore syringe is a PITA. He's going to be pleasantly surprised when he sees that working with agar is less messy and far more effective.

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Shroomboy5]
    #23165772 - 04/28/16 04:13 PM (8 years, 8 months ago)

Quote:

Shroomboy5 said:
Alright man. Is the Agar powder safe to ship to CA? Looks like that's 30-40 dollars. And do you know where I can get the petris?



Yes agar is not only used to grow illegal mushrooms. Nothing sketchy about it

Agar agar on amazon - $6
Boxed potatoes - 2$
Honey - free if you got it
Glad mini rounds - $4 for 8

That's all you need. If they are charging $40 fuck that.


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OfflineShroomboy5
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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: SloppyJoseph]
    #23165826 - 04/28/16 04:25 PM (8 years, 8 months ago)

Wait a second, since I have a spore syringe can I create whatever is made with that syringe? Because in one of the videos after the agar mixture is poured into a container the next day the guy put one drop from a syringe into the agar.

If this way is possible and better than waiting to get a new cap, would it be okay to put more drops into the agar mixture if my syringe is weak>

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Shroomboy5]
    #23165848 - 04/28/16 04:29 PM (8 years, 8 months ago)

If you still have drops left in the syringe you are golden. One drop has millions of spores in it, two drops doesn't make a differen e, it's overkill. The best thing to do is do 1 drop on 6 or so plates to increase your chances of success. Sometimes the spores don't want to germinate.


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Shroomboy5]
    #23165873 - 04/28/16 04:33 PM (8 years, 8 months ago)

Nope, with agar less is more. The more spore solution you squirt in the plate the higher your chances of contamination. One drop is more than enough.

Keep watching the vids till you see the one in which he's inoculating the agar plate with a swab. I'd do that in your situation, since you already have a print and it's better and less messy to inoculate agar with a spore print than with spore solution.

Why do you think your syringe is weak? If you can't see the spores that doesn't mean is weak. Spore syringes are almost always greatly overloaded with spores. You could count thousands if not millions of spores in a single drop.
Anyway, I really think spore print to agar is your best option.

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: SloppyJoseph]
    #23165884 - 04/28/16 04:37 PM (8 years, 8 months ago)

Dude, I have over 7 ML of the GT syringe. If this is true I'm way more confident now!

The only thing is that I inoculated 3 jars with this syringe in back in mid-February, 1 Ml in each, and only one jar colonized and from only one spot. That's my small concern.

But hey If all I need is one agar container to colonized and I can clone from that then I can keep trying just one drop until I get a container to colonize.

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Josex]
    #23165887 - 04/28/16 04:38 PM (8 years, 8 months ago)

BTW OP, check out this link as well. You'll see how easy it is to inoc an agar plate with a spore print.

https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=20111637&fpart=1&PHPSESSID=

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OfflineShroomboy5
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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Josex]
    #23165900 - 04/28/16 04:42 PM (8 years, 8 months ago)

I thought that the print I made were mostly probably contaminated..? I'm not sure if I know which video you're saying but I'll watch them all.

Mine is weak cause it's almost a year old and only colonized one jar out of three.

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Shroomboy5]
    #23165921 - 04/28/16 04:47 PM (8 years, 8 months ago)

Quote:

Shroomboy5 said:
I thought that the print I made were mostly probably contaminated..? I'm not sure if I know which video you're saying but I'll watch them all.

Mine is weak cause it's almost a year old and only colonized one jar out of three.




Well your print might have contams, but the purpose of agar is that it allows you to isolate healty mycelium away from any possible contamination. I think all those vids and links will answer most of your questions, but if you still have any questions after reading them, you can make a new thread to ask.

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OfflineShroomboy5
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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Josex]
    #23165930 - 04/28/16 04:51 PM (8 years, 8 months ago)

To me it sounds like this is what you're saying.

My spore prints are mostly likely contaminated.

But if I scrap some off with a cotton swab onto some ready agar mixture, if it is contaminated it will cultivate both healthy mycelium and contaminated.

Then I cut a part of the healthy part of the mycelium from that agar container, and drop it into a new agar container? And viola healthy mycelium ready to colonize in a jar?

So I'm basically 100% set since I have a bunch of prints and a syringe..

Edited by Shroomboy5 (04/28/16 04:52 PM)

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Shroomboy5]
    #23165975 - 04/28/16 05:10 PM (8 years, 8 months ago)

Quote:

Shroomboy5 said:
To me it sounds like this is what you're saying.

My spore prints are mostly likely contaminated.

But if I scrap some off with a cotton swab onto some ready agar mixture, if it is contaminated it will cultivate both healthy mycelium and contaminated.

Then I cut a part of the healthy part of the mycelium from that agar container, and drop it into a new agar container? And viola healthy mycelium ready to colonize in a jar?

So I'm basically 100% set since I have a bunch of prints and a syringe..



:thatsayes:

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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: SloppyJoseph]
    #23166043 - 04/28/16 05:29 PM (8 years, 8 months ago)

Quote:

SloppyJoseph said:
Quote:

Shroomboy5 said:
Alright man. Is the Agar powder safe to ship to CA? Looks like that's 30-40 dollars. And do you know where I can get the petris?



Yes agar is not only used to grow illegal mushrooms. Nothing sketchy about it

Agar agar on amazon - $6
Boxed potatoes - 2$
Honey - free if you got it
Glad mini rounds - $4 for 8

That's all you need. If they are charging $40 fuck that.



I started with agar too, it's not hard once you do it a few times.


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: MysticMoteToter]
    #23166784 - 04/28/16 08:11 PM (8 years, 8 months ago)

I think agar is abundant in California. It is used a lot in Asian cuisine


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Mad Season]
    #23166864 - 04/28/16 08:33 PM (8 years, 8 months ago)

I like MEA. $44 per lb after shipping is pretty good. Makes about 300 dishes give or take. I like how it performs over PDA. I've used a lot of both and MEA always seems to have the superior results.


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: Josex]
    #23167487 - 04/29/16 12:14 AM (8 years, 8 months ago)

Quote:

Josex said:
Quote:

Shroomboy5 said:
To me it sounds like this is what you're saying.

My spore prints are mostly likely contaminated.

But if I scrap some off with a cotton swab onto some ready agar mixture, if it is contaminated it will cultivate both healthy mycelium and contaminated.

Then I cut a part of the healthy part of the mycelium from that agar container, and drop it into a new agar container? And viola healthy mycelium ready to colonize in a jar?

So I'm basically 100% set since I have a bunch of prints and a syringe..



:thatsayes:



:thatsayes:


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: PirateSwazey]
    #23168122 - 04/29/16 06:35 AM (8 years, 8 months ago)

Quote:

PirateSwazey said:
Quote:

Josex said:
Quote:

Shroomboy5 said:
To me it sounds like this is what you're saying.

My spore prints are mostly likely contaminated.

But if I scrap some off with a cotton swab onto some ready agar mixture, if it is contaminated it will cultivate both healthy mycelium and contaminated.

Then I cut a part of the healthy part of the mycelium from that agar container, and drop it into a new agar container? And viola healthy mycelium ready to colonize in a jar?

So I'm basically 100% set since I have a bunch of prints and a syringe..



:thatsayes:



:thatsayes:



:yes:


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Re: Easy way to inoculate for a lazy, low confidence guy? [Re: SloppyJoseph]
    #23168133 - 04/29/16 06:40 AM (8 years, 8 months ago)

not sure if someone linked this yet, but these are Pasty Plates and are supper easy. agar is a lot less complicated than it seems. i made lots of plates before my first grow was even fruiting.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

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