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InvisibleDoxx

Registered: 02/23/16
Posts: 146
Re: MS straight to agar for isolation: waste of time? [Re: Lt.Berkenstine] * 1
    #23155490 - 04/25/16 10:56 PM (8 years, 8 months ago)

My first time MS to Agar.

This took about 5 transfers from a grain inoculated with MS.



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OfflineLt.Berkenstine
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Registered: 03/10/16
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Re: MS straight to agar for isolation: waste of time? [Re: Doxx]
    #23155563 - 04/25/16 11:34 PM (8 years, 8 months ago)

I see. Nice work man! Looks beautiful. Hope I see a yield like that. I should have used a grain to knock up my dishes fosho. Still unsure whether to buy new agar or use what I have though


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B+ outdoor bouquet                GT monotub monster
           

Don't sass me.

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InvisibleMoabfighter
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Re: MS straight to agar for isolation: waste of time? [Re: Doxx]
    #23155572 - 04/25/16 11:37 PM (8 years, 8 months ago)

Quote:

Doxx said:
My first time MS to Agar.

This took about 5 transfers from a grain inoculated with MS.








Man. I'm very jealous. I really wish I could produce that, and I mean that with all due respect. Time and patience will lead me to this,I know it will.

I don't do this for profit or money, it's simply "cool" to me. I very much enjoy it. Only done MS syringes and pf cakes, one or two monotubs. I can't wait to learn more and grow all if mushrooms like you. That's so neat. Props :smile:


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KSSS And PE WBS.
 

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Offlinespacechildo
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Re: MS straight to agar for isolation: waste of time? [Re: Moabfighter]
    #23156212 - 04/26/16 07:45 AM (8 years, 8 months ago)

your dishes looks messy because you never did a transfer. in stro's pics you can even see the colonized grains right in the center of his plates.

If there is a question in there I missed just ask again, putting spores to agar is a pretty simple thing.

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Invisiblemupetmower
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Registered: 03/29/16
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Re: MS straight to agar for isolation: waste of time? [Re: Lt.Berkenstine] * 1
    #23156406 - 04/26/16 09:04 AM (8 years, 8 months ago)

But, Space was actually answering that question about the isolating. when you start with multi-spore to agar, no matter what, you are going to need to make a good deal of transfers before you will even begin to see sectors form to where you can start trying for isolation. also, to get any good isolation, you are going to need to clean up the myc like he was advising, also. because multi-spore to agar from a spore syringe is not going to be 100% clean. so as Space said, make some transfers when you start seeing growth, clean it up, then you will eventually start seeing sectoring, and be able to try making isolation. as for the water content thing, it should be about the same as if you used a recipe from scratch. and both will work, lab grade or scratch, whether it is made from malt extract or made with potato flakes.

also, about how it should look for MS, do as Space said, put a drop in the middle. you will see it grow from that spot. it probably isnt going to look super rhizo, but instead will fan out in a cricle and then as i said, after multiple transfers it will start to appear more rhizo and start to form sectoring.

Space knows what he is talking about. he wont lead you wrong.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

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OfflineLogicaL ChaosM
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Re: MS straight to agar for isolation: waste of time? [Re: mupetmower]
    #23156584 - 04/26/16 10:06 AM (8 years, 8 months ago)

Quote:

mupetmower said:
But, Space was actually answering that question about the isolating. when you start with multi-spore to agar, no matter what, you are going to need to make a good deal of transfers before you will even begin to see sectors form to where you can start trying for isolation. also, to get any good isolation, you are going to need to clean up the myc like he was advising, also. because multi-spore to agar from a spore syringe is not going to be 100% clean. so as Space said, make some transfers when you start seeing growth, clean it up, then you will eventually start seeing sectoring, and be able to try making isolation. as for the water content thing, it should be about the same as if you used a recipe from scratch. and both will work, lab grade or scratch, whether it is made from malt extract or made with potato flakes.

also, about how it should look for MS, do as Space said, put a drop in the middle. you will see it grow from that spot. it probably isnt going to look super rhizo, but instead will fan out in a cricle and then as i said, after multiple transfers it will start to appear more rhizo and start to form sectoring.

Space knows what he is talking about. he wont lead you wrong.




QFR :takingnotes:

What do u guys think is better MS to fruit then clone on agar or MS to agar then isolate?

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Offlinespacechildo
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Re: MS straight to agar for isolation: waste of time? [Re: LogicaL Chaos] * 1
    #23156601 - 04/26/16 10:12 AM (8 years, 8 months ago)

better how? fastest? most aggressive culture?
an isolate is just that, an isolate. its not automatically better or worse than MS, its just consistent.
MS is like rolling a dice. an isolate is like you always get the same outcome of the dice. no one can say if its a consistent 6 or 1 or even 3.

cleaning on agar, waiting for in vitro pin, putting that pin on agar, then to grains is what I like to do.

and to the OP if the main question was if your agar recipe is off, I'd say no, its solidified and you got germination and growth.
anywhere from 1-3% agar is good, you can probably go way higher too.

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Invisiblemupetmower
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Re: MS straight to agar for isolation: waste of time? [Re: spacechildo]
    #23156709 - 04/26/16 10:42 AM (8 years, 8 months ago)

hey logical, i went MS to brf cakes, and then am taking clone smaples from the best fruits to agar, as well and some spore prints on foil for agar later. i also inoc'd some plates with the same MS syringe, so it is being transfered and cleaned up right now. like space said, if you mean the fastest way, then prolly MS to brf cakes, then you have fruits that you can choose to clone and print, then start the agar process, or do like i did, and do it both ways.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

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OfflineLt.Berkenstine
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Re: MS straight to agar for isolation: waste of time? [Re: mupetmower]
    #23163640 - 04/28/16 12:19 AM (8 years, 8 months ago)

Not like this thread needs another bump, but Thank you guys so much for answering after my whilpersnapper comment there. I think I'm gunna keep the neater looking dishes just in case I don't have a nice fruit to clone from. But...

In a nutshell, I think I would say that MS straight to agar isn't the best idea. Doesn't mean much coming from me, but it's definitely the shittiest method of cleaning and iisolation in my humble opinion. I would personally knock up a jar and then put the grain on agar. I've already had much better results on dishes from doing this.. In my personal scenario, with the way I think, I like the idea of going from fruit to agar better. The only problem with this for the beginner is getting to the point of fruiting without having a 100% clean inoculant.

It seems like a viscous circle for a beginner to get clean myc sometimes, but the way I made sense of it is going grain to agar.

I'd like to see someone else's plates after their first innoc or even their first transfer from straight MS and see if it looks any better.


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B+ outdoor bouquet                GT monotub monster
           

Don't sass me.

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InvisiblePastywhyteMDiscord
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Re: MS straight to agar for isolation: waste of time? [Re: Lt.Berkenstine]
    #23164143 - 04/28/16 06:50 AM (8 years, 8 months ago)

I have done spores to agar from both prints and syringes (swabs too) dozens of times with great success. I'm not sure what you are talking about. Spores to agar is the best way to germinate spores.

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OfflineLt.Berkenstine
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Re: MS straight to agar for isolation: waste of time? [Re: Pastywhyte]
    #23167038 - 04/28/16 09:27 PM (8 years, 8 months ago)

I guess I was looking for a certain type of growth and didn't get it. I did however get much nicer looking plates from colIbises grain though.

It's not like my failure to get what I was looking for proves anything I guess.

How did your growth look with MS to agar? How long did it take to see growth? The usual? Cause these bad boys, with a couple exceptions, took longer than expected


--------------------
B+ outdoor bouquet                GT monotub monster
           

Don't sass me.

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OfflineLt.Berkenstine
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Re: MS straight to agar for isolation: waste of time? [Re: Lt.Berkenstine]
    #23167041 - 04/28/16 09:28 PM (8 years, 8 months ago)

The smell of the inside of my bag of dishes might also indicate that I goofed somewhere with my sterile procedure :/


--------------------
B+ outdoor bouquet                GT monotub monster
           

Don't sass me.

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