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MushroomBilly
Explorer


Registered: 03/24/13
Posts: 374
Last seen: 7 years, 5 months
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Re: invitro poo bags [Re: soli]
#23161556 - 04/27/16 02:00 PM (8 years, 8 months ago) |
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Just leave them in the light. They will grow.
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soli
rogue psychologist



Registered: 05/18/06
Posts: 280
Last seen: 8 years, 7 months
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OK. So Im gonna just do that, set the temp around 69 70F to initiate pinning, in a plastic sheeted room...
Is it okay to have an ultra sonic cool mist humidifier running in the fruiting room to increase FAE? I know moving air can be bad but it'll be in a sealed environment.
Again, I'm still waiting on answers regarding using MGMC (gretchen taught me that it is a great bulk substrate but ive only used it in a martha before....has coir etc in it) ... after I pasteurize the bag from lowes, does anyone think that may be a good alternative to use in my poo bags? I really hate monotubs, and I'm gonna make another martha once I get the money for it..
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: invitro poo bags [Re: soli]
#23162083 - 04/27/16 05:04 PM (8 years, 8 months ago) |
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your bags dont look healthy to me, bacteria probably stopped them from colonizing. miracle grow from monsanto? why not just buy coir and verm separately and not support one of the worst companies the world has seen?
for you Q, sub dont care what you fruit it in, you want RH and FAE whether its mgmc,coir,manure,straw etc..
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soli
rogue psychologist



Registered: 05/18/06
Posts: 280
Last seen: 8 years, 7 months
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well
i'm going to try MGMC in bags ... and I'm just going to collect my own manure and straw like I used to rather than from an industrial farm for making more bags.
I really hate monotubs so I'll see how well MGMC works in the bags. I imagine i'll have to pasteurize it as well?
I guess I'll find out in a week what happens in the bags because I'm going to start the fruiting process tonight at low temp in light..
Any other advice / input is deeply appreciated. I used to be soooo good at this but I've done a lot of drugs inbetween and forgot a lot of my old methods
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: invitro poo bags [Re: soli]
#23163596 - 04/27/16 11:59 PM (8 years, 8 months ago) |
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Stalled substrate is usually caused by bacteria, regardless if you can see the signs of it or not. It seems like they have means of GE so I can't think of anything else that might make them stall.
Not mixing the substrate well enough is a moot point because they will still colonize, albeit slower. You should still see progress in colonisation even with the worst shake ever.
But if it as you say, that after 2 weeks they seem to be the same, I guess it is time to open them and see what they smell like. If bacteria did indeed stall them, they should have a horrid smell.
Putting stalled bags/jars in an incubator will only make things worse by the way (unless you live in a very cold climate). Higher temperatures promote bacterial growth so if the mycelium is competing with bacteria for the substrate, putting it in an incubator will only give the bacteria the upper hand. IMO you need to look for the problem at it's root, which could be in a lot of places. Could be bulk quality, sub-par pasteurization, bad spawn, sub-par sterile tek etc. You basically have to look at all your steps up to that point under a microscope to see what is making your substrate stall.
Keep in mind that if you are buying compost from a mushroom farm, it could very well be already used compost. Those "mushroom compost" bags you find in stores and supermarkets are just spent compost. You are better off using regular compost if that is the case. I don't have any experience with compost as a bulk and it seems like it isn't used anymore by homegrowers, maybe it has something to do with that.
If the MGMC has any plant fertilizers in it, you should refrain from using it. Fungi are a totally different organism than plants and tests using miracle grow as a nutrient boost show that they do not perform as good on it. Not too long ago there was a guy testing an LC using some type of miracle grow, suffice it to say, it did not go well.
You should use regular coir IMHO, cubensis do better with less nutrients anyway.
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soli
rogue psychologist



Registered: 05/18/06
Posts: 280
Last seen: 8 years, 7 months
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Quote:
Supalemonhaze said: Stalled substrate is usually caused by bacteria, regardless if you can see the signs of it or not. It seems like they have means of GE so I can't think of anything else that might make them stall.
Not mixing the substrate well enough is a moot point because they will still colonize, albeit slower. You should still see progress in colonisation even with the worst shake ever.
But if it as you say, that after 2 weeks they seem to be the same, I guess it is time to open them and see what they smell like. If bacteria did indeed stall them, they should have a horrid smell.
Putting stalled bags/jars in an incubator will only make things worse by the way (unless you live in a very cold climate). Higher temperatures promote bacterial growth so if the mycelium is competing with bacteria for the substrate, putting it in an incubator will only give the bacteria the upper hand. IMO you need to look for the problem at it's root, which could be in a lot of places. Could be bulk quality, sub-par pasteurization, bad spawn, sub-par sterile tek etc. You basically have to look at all your steps up to that point under a microscope to see what is making your substrate stall.
Keep in mind that if you are buying compost from a mushroom farm, it could very well be already used compost. Those "mushroom compost" bags you find in stores and supermarkets are just spent compost. You are better off using regular compost if that is the case. I don't have any experience with compost as a bulk and it seems like it isn't used anymore by homegrowers, maybe it has something to do with that.
If the MGMC has any plant fertilizers in it, you should refrain from using it. Fungi are a totally different organism than plants and tests using miracle grow as a nutrient boost show that they do not perform as good on it. Not too long ago there was a guy testing an LC using some type of miracle grow, suffice it to say, it did not go well.
You should use regular coir IMHO, cubensis do better with less nutrients anyway.
well, the MGMC contains coir and possibly verm, i dont remember, but i do recall it working wonderfully in trays. I'll open up one of the bags tho and see how they smell. Im hoping to get at least SOME mushrooms...
the compost i got was not spent , it was already pasteurized and ready to go , however the jars were probably a tad stalled since they were ike 2 weeks colonized before i spawned. I also re-pasteurized the compost to be on the safe side.
I think Im just going to hit up a horse farm, get some straw and make my own 50/50 mix like I used to, as well as buy MGMC n pasteurize both, and do some side-by-side's in both bags in tubs. I dont know what else to do... coir is too expensive for me anyways, MGMC works great , at least in trays like I said.
Regarding the source of my compost, I live in Kennet Square , PA, which is known as the mushroom capital of the world and supplies over 50% of mushrooms in america. I got the pasteurized, unspent compost from one of the farms. Also, the jars did NOT smell funny when I spawned them. Perhaps it was just a lack of FAE?
Again, despite that I am going to attempt fruiting them anyways in a plastic sheeted 'clean room' , is it okay to have an ultra sonic cool mist running in there? I know air movement is bad, but I forgot if its bad at this stage. Back when I was doing marthas, I had two humidifiers running at the same time , plugged into 2 setups with hoses...
IIRC, a 42qt (one of the smaller ones) monotub should have about 4 pints and be about 4 inches tall?
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