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OfflineFalseMaria
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Injector Port - Is SAB or Flow Hood Required For Inoculation?
    #23156945 - 04/26/16 11:51 AM (8 years, 8 months ago)

I tried searching for the answer, but found nothing, and I'm on my phone so further searching makes my head hurt.

I have 8 jars of WBS sterilised and ready for inoculation. They all have SFD for gas exchange and red self healing ports.

I do have a flow hood, but was curious if it's needed with port inoculation. Obviously flame sterilise the needle, but other than that, would I be safe to do it without the flow hood?

This is more out of curiosity. I am back at the hobby after several years off. Trying to clear the cobwebs out.


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Offlinespacechildo
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: FalseMaria]
    #23156973 - 04/26/16 12:01 PM (8 years, 8 months ago)

you really should use one, yeah. nasties can land on your SHIP and get pushed into the jar during inoc. just cracking the lid open in a sab/fh is more consistent than using SHIPs IME.

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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: spacechildo]
    #23156981 - 04/26/16 12:02 PM (8 years, 8 months ago)

Quote:

spacechildo said:
you really should use one, yeah. nasties can land on your SHIP and get pushed into the jar during inoc. just cracking the lid open in a sab/fh is more consistent than using SHIPs IME.



:whathesaid:

SHIP = direct contact

in my book, that is a bad thing.

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OfflineFalseMaria
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: Munchauzen]
    #23157009 - 04/26/16 12:10 PM (8 years, 8 months ago)

Sweet, thanks for clearing that up. The flow hood is not super accessible right now, so had you said the opposite, I would have not bothered. I will bother now :wink:


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OfflineKingBumi
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: FalseMaria]
    #23157111 - 04/26/16 12:39 PM (8 years, 8 months ago)

If you have a flow hood, use it!

Otherwise no it's not necessary, I didn't have one my first time and it worked out very well, even in this dirty place.

Have the foil ready to kick off, red needle, into alcohol wipe. Push off foil and seamlessly needle goes straight from the wipe into the hole. That'll do it.


Be careful with WBS by the way. I got a dirty batch with some impossible to kill bacillus species in there and I've been traumatized ever since.

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OfflineFalseMaria
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: KingBumi]
    #23157168 - 04/26/16 12:52 PM (8 years, 8 months ago)

I don't have foil since I have synthetic filter discs glued on the lid for gas exchange.

My last 3 grows with WBS have been plagued by mold ruining stuff during my first flush. But I think my substrate was too deep, and I was doing all my bulk stuff in an unfinished basement. Even happened when I skipped the bucket tek to do proper pasteurisation if the bulk sub (coir/verm). This is partly why it's been a few years since I tried. I was discouraged.

I was thinking of just casing the 8 jars, and skipping the bulk part this time around. Just so I can have a success under my belt again.


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Offlinespacechildo
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: FalseMaria]
    #23157186 - 04/26/16 12:56 PM (8 years, 8 months ago)

probably dirty spawn jars, if you start your spores on agar you can clean them up and ensure clean inoculant.
dont do what that dude just said, its terrible procedure.

a simple plastic box with 2 arm holes = SAB, so easy there's no reason not to use one.

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OfflineFalseMaria
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: spacechildo]
    #23157208 - 04/26/16 01:03 PM (8 years, 8 months ago)

Well, I have a flow hood, so I won't do the SAB method this time. My basement is days away from completion, so agar work will be possible next try (very soon), I just wanted to get a head start with MS to WBS.

Last grows I did WBS with G2G, and I still think my spawn smelled like mushrooms. This is kind of why I want to go the fastest, most direct route and see if I can succeed this way.


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Offlinespacechildo
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: FalseMaria]
    #23157220 - 04/26/16 01:08 PM (8 years, 8 months ago)

yeah a FH is a good reason not to need a SAB :wink::thumbup:

If you're doing spores to grains just use very little spore solution. a few drops against the glass where it meets the grains is all you need and want. no syringes are 100% clean so try and minimize the chance of getting something nasty in your jars.

there may still be problems growing in your jars even tho you cant smell it,
but a nasty smell is a dead give away they're fucked.

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OfflineFalseMaria
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: spacechildo]
    #23157234 - 04/26/16 01:14 PM (8 years, 8 months ago)

Even with multiple grows I am no expert, but each time, even after G2G, the spawn smelled fine to me. Thanks for the tip about using tiny amounts of inoculant.


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Offlinespacechildo
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: FalseMaria]
    #23157245 - 04/26/16 01:18 PM (8 years, 8 months ago)

post pics of your grain jars before spawning or g2g'ing them, bacteria doesnt necessarily make a smell but you can see it because of how thick and off it grows. it only takes 1 bacterial jar to fuck up an entire tub.

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OfflineFalseMaria
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: spacechildo]
    #23157256 - 04/26/16 01:20 PM (8 years, 8 months ago)

Will do, stay tuned.


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OfflineFalseMaria
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: FalseMaria]
    #23157584 - 04/26/16 03:21 PM (8 years, 8 months ago)

Update #1:

So I inoculated my jars a few mins ago. I shut off all ventilation, ran the flow hood for 25 mins, took a shower, changed into fresh clothes, and got to work. Wiped the jars and injector ports with alcohol/lysol wipes. There were only two problems that came up.

The first is that the spores in the syringe was all in one chunk. Try as I might to break it up, I only got it so far. I am sure it was fine since so many spores are likely invisible, but that was a bit frustrating. Not knowing if I could suck up air into the syringe in front of the flow hood to give me more room to shake the water around, I just accepted how much I was able to break up and went ahead.

The second issue was my alcohol lamp. I was unable to get it to light. It's one of the wickless ones with the copper tubing. At any rate, I decided to go ahead and flame sterilise with the lighter I had on hand. NOt the ideal situation, but I decided to go ahead anyways.

So I inoculated each jar with as few drops as possible. For 8 jars I may have used 1cc-2cc. There's a sticker wrapped around the syringe, so I can't read the volume level.

The only other thing I can say I noticed was some of my grains have burst. Either during the simmering prior to sterilisation, or during the sterilisation process. Either way it's not a big amount, and the grains are not sticky or mushy. They might be only slightly wetter than what the ideal is, but any grains that stick to the glass are easily tapped off, and there is no water pooling at the bottom of the jars.

I'll post some pics once I see some initial growth.


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OfflineAusMush
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: FalseMaria]
    #23157698 - 04/26/16 03:54 PM (8 years, 8 months ago)

Hmmm I agree with everyone except for the peeps that said cracking the lid is better in a sab I actually do both (use ships) in a sab . The ships and the entire jar is sprayed with a diluted bleach then the lid is wiped with an alcohol swab, the needle is then flamed red hot . Also! Forgot to mention that cracking a jar lid can be finicky too do sometimes and creates an eddy-current a suction of air into the jar .

Each to there own I suppose :thumbup:

Edited by AusMush (04/26/16 03:55 PM)

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OfflineKingBumi
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: FalseMaria]
    #23157742 - 04/26/16 04:08 PM (8 years, 8 months ago)

Yeah OP, you got it. It's all just a waiting game now, take it easy and let yourself ease back in.

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Offlinespacechildo
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: AusMush]
    #23157754 - 04/26/16 04:11 PM (8 years, 8 months ago)

alcohol and bleach kills 99,99% of all germs, 0,001 x 100 million = eeeeew! :lol: there's a pic somewhere but I cant find it right now..

as long as you got some spores into each jar I dont see any reason why this wouldn't work mary..

Quote:

AusMush said:
creates an eddy-current a suction of air into the jar .




no suction or draft's created by opening a jar, only way this would happen is if the jar created a vacuum during the PC cycle which wont happen if you have a hole in the lid.

Edited by spacechildo (04/26/16 04:13 PM)

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OfflineAusMush
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: spacechildo]
    #23157871 - 04/26/16 04:45 PM (8 years, 8 months ago)

:stoned: I live in a filthy house and I have seen a massive reduction in d contamination :-) just my 2c worth bro no point in opening a jar of you don't need to , with the exception of having a flow hood.

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Offlinespacechildo
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: AusMush]
    #23157888 - 04/26/16 04:49 PM (8 years, 8 months ago)

no point in having your needle touch your jar if you dont need to :shrug:
your needle is clean, the SHIP is not, why have a clean needle touch a dirty SHIP before inoculating?
If you see problems from opening your jars in a SAB you need to look into your sterile technique, opening the lid is the only way to get agar wedges into the jar and is something everyone should learn.

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OfflineAusMush
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: spacechildo]
    #23157919 - 04/26/16 04:58 PM (8 years, 8 months ago)

So your saying a pressure cooked jar with a aluminum foil square wrapped onto the lid, then the red hot needle touching the SHIP for a few seconds is not sterile? I have to 're evaluate my sterile technique then, I guess the stuff I have been reading is garbage ? Can I get some links to some reading material? Always like to improve my technique. Thanks :-) :thumbup:

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Offlinespacechildo
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Re: Injector Port - Is SAB or Flow Hood Required For Inoculation? [Re: AusMush]
    #23157940 - 04/26/16 05:03 PM (8 years, 8 months ago)

you take the jar out of the PC and its no longer sterile.

frankhorrigan has some SAB tech info in his TEK list and transcendinglife has a nice video on g2g transfers.
Other than that, IDK man, flame red hot sterilizes, ISO santizes, anything in open air isn't sterile anymore, just add that to your info and you should be golden :thumbup:

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