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OfflineBertoxulous
Stranger

Registered: 04/26/16
Posts: 23
Last seen: 8 years, 8 months
Re: Old timer returning, Help with Simplicity & Efficiency [Re: YaMoonSun]
    #23158738 - 04/26/16 07:39 PM (8 years, 8 months ago)

Why bother germinating/colonizing on agar, then transferring to grain when I can just germinate/colonize on the grain to begin with?

Seems like an unnecessary extra step / risk of contam.

Making a syringe is fast.

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OfflineYaMoonSun
The Double Standard

Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 1 year, 4 months
Re: Old timer returning, Help with Simplicity & Efficiency [Re: Bertoxulous]
    #23158756 - 04/26/16 07:43 PM (8 years, 8 months ago)

If you do it in a SAB, any contaminate that you'll encounter was likely already inside of the spore print. Then you can take clean mycelium from the petri (Pasty plates) and transfer to another.

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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
Re: Old timer returning, Help with Simplicity & Efficiency [Re: Bertoxulous]
    #23158764 - 04/26/16 07:44 PM (8 years, 8 months ago)

no spore prints are 100% clean so agar ensures clean inoculant.
you'd still need a SAB to make the spore syringe. you can get away with open air inocs through SHIPs but its not good practice.

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OfflineBertoxulous
Stranger

Registered: 04/26/16
Posts: 23
Last seen: 8 years, 8 months
Re: Old timer returning, Help with Simplicity & Efficiency [Re: YaMoonSun]
    #23158772 - 04/26/16 07:45 PM (8 years, 8 months ago)

Yeah, I was going to start working with agar when I stopped growing years ago, but for this next project, I don't really want to invest in plates and other supplies I don't have at the moment.

I just want to grow some fruits as quickly and cost effectively as I can for the next 3 months before I have to move.

Then once I settle down maybe I will delve into agar.

*EDIT*

Hmmm, I see the point you all are making now. Will be a great waste of time if I think I have clean colonized jars then when I go to spawn, find out that they weren't clean because my innoculant was contammed.

Maybe I'll buy some plates and medium then.

Any recommendations on where/what kind of plates to get for cheap starter plates?

Edited by Bertoxulous (04/26/16 07:55 PM)

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Invisiblefilthyknees
no coincidence
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Registered: 03/08/13
Posts: 6,283
Trusted Cultivator
Re: Old timer returning, Help with Simplicity & Efficiency [Re: Bertoxulous]
    #23158887 - 04/26/16 08:09 PM (8 years, 8 months ago)

https://www.shroomery.org/forums/showflat.php/Number/19208976
one bottle of premix agar is like 15 bux or something. get like 20+ of the minirounds so you have blanks waiting to transfer to.

<$50 investment & it will return so much moar


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night

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OfflineBertoxulous
Stranger

Registered: 04/26/16
Posts: 23
Last seen: 8 years, 8 months
Re: Old timer returning, Help with Simplicity & Efficiency [Re: Bertoxulous]
    #23158903 - 04/26/16 08:13 PM (8 years, 8 months ago)

If I decide to do agar, can I go straight from a print to the agar?

My prints are so old, someone had mentioned giving them time to hydrate if I made syringes.

Will they be able to hydrate if I go straight from a print to agar?

What would be a good method for that transfer? A flame sterilized scalpel/razor and just scrape some spores up and then wipe them on the agar? Or cut into the agar to deposit spores?

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Invisiblefilthyknees
no coincidence
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Registered: 03/08/13
Posts: 6,283
Trusted Cultivator
Re: Old timer returning, Help with Simplicity & Efficiency [Re: Bertoxulous]
    #23158920 - 04/26/16 08:16 PM (8 years, 8 months ago)

print to agar :thumbup::thumbup:

for transfer I like sterile cotton swabs (no flame sterilizing). just a light drag will do.

edit: the print he used there was 5+ years old so it's worth a shot


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night

Edited by filthyknees (04/26/16 08:23 PM)

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OfflineBertoxulous
Stranger

Registered: 04/26/16
Posts: 23
Last seen: 8 years, 8 months
Re: Old timer returning, Help with Simplicity & Efficiency [Re: filthyknees]
    #23158975 - 04/26/16 08:31 PM (8 years, 8 months ago)

Awesome!

Thank you guys so much for the info and suggestions. It's great to see the community is still very active and helpful after all these years.
Cheers!

I guess I'm going agar after all.
Time to make a shopping list!

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OfflineBertoxulous
Stranger

Registered: 04/26/16
Posts: 23
Last seen: 8 years, 8 months
Re: Old timer returning, Help with Simplicity & Efficiency [Re: Bertoxulous]
    #23159174 - 04/26/16 09:07 PM (8 years, 8 months ago)

Anyone have suggestions on affordable tyvek sleeves?
Best price I can find on amazon is almost $2 per pair.

Or can I just reuse 1 pair? I've never used the thing, I just used to wipe my arms with alcohol.

Edited by Bertoxulous (04/26/16 09:08 PM)

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Offlinetombosley8
Full on... Bossley Baggins
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Registered: 10/14/13
Posts: 3,660
Last seen: 6 months, 13 days
Re: Old timer returning, Help with Simplicity & Efficiency [Re: Bertoxulous]
    #23159436 - 04/26/16 09:57 PM (8 years, 8 months ago)

If you wrap them in foil (I roll them up then wrap them in foil)and then PC for 30 min they will be sterile for each use. I have a few pairs that I wrap as stated above and then pc all at once for the next few sab procedures. You can place the freshly pc'd rolls into a new ziploc bag to keep them clean until you need them.

Bare arms are fine as long as you wash and alcohol very well but I just feel more safe with them on.


--------------------

Edited by tombosley8 (04/26/16 09:58 PM)

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OfflineBertoxulous
Stranger

Registered: 04/26/16
Posts: 23
Last seen: 8 years, 8 months
Re: Old timer returning, Help with Simplicity & Efficiency [Re: tombosley8]
    #23160327 - 04/27/16 04:59 AM (8 years, 8 months ago)

Cheers!

If only there were somewhere local to buy them.

I don't like buying stuff online

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