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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Agar, Herb Grinder, Pressure Cooker

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OfflineGratefulDread
Never pass up anopportunity topee.

Registered: 10/21/02
Posts: 366
Last seen: 12 years, 2 months
Honey Water Cloning
    #2315070 - 02/08/04 07:01 PM (12 years, 9 months ago)

HONEY WATER CLONING TEK

  This TEK is actually just a piecing together of other established TEKs with a few small additions by myself. I'm merely posting this as someone had a question about the viability of using Honey water or Karo water for cloning. I have done this many times with excellent results as finding Agar is a pain in my area. I do 6 jars at a time just in case of contaminants so you always have spares and it costs next to nothing to do  this.

Needed:
6 250 ml jars. 1 tsp organic honey per jar. Distilled water.  Coffee grinder or knife. Hydrogen Peroxide 3%. 10cc Syringe. Pressure Cooker. Lysol. A clean stem from whatever mushroom you want to clone

Tek:
-Clean all work areas thoroughly and spray down with Lysol and wipe with a clean cloth or paper towel
-Punch a small hole in the lid of each of the jars big enough for the syringe needle to pass through.
-Fill jars 3/4 full of warmed water
-Add about 1tsp of organic honey and stir in
-Put lids on jars upside down so the rubber is pointed up
-Pressure cook for 30 min at 15 PSI
-Set in sterile place to cool
-Wash hands and sterilize your hands, the knife, and/or coffee grinder and all surfaces with Lysol
-Take stem of fruitbody to be cloned and macerate with knife in a sterile area or run through a sterilized coffee grinder
-Place macerated fruitbody into about 1/3c of Hydrogen Peroxide and let fizz.
-Sterilize syringe tip and draw 10 cc of the mixture into the syringe trying to get as much mycellium into the syringe as possible.
-Innoculate each of the jars with 1 to 1.5 cc of mycellium mixture
-Set in dark sterile area to colonize at about 80 degrees

    You should see mycellium growth within 24 to 36 hours. After you start to see alot of whispy white mycellium floating in the liquid (Approx 3-4 days) add 2cc's of peroxide and refrigerate for use. Any color growth besides white is a contaminant and you should just empty the jar. The honey water can be used for about 2 weeks and then it should be discarded as the chances of contaminants increase after that time. To use the honey water just sterilize a syringe, poke it through the hole in the top of the jar and draw out the liquid getting as much mycellium into the syringe as possible. Use 2-3cc of liquid for every jar of substrate you want to innoculate.

Additions:
-You can add broken glass to the jars so that after the mycellium starts to grow you can shake the jars and break up the mycellium allowing you to get smaller pieces allowing you to get more into your syringe.
- sealing the hole in the lid after the jars cool with a glue gun or tape will drastically reduce the possibilities of contamination


  Any comments, suggestions, additions, and flames welcomed and encouraged.
:laugh:

-GD


--------------------
Before you criticize someone, you should walk a mile in their shoes.
That way, when you criticize them, you're a mile away and you have their shoes.


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InvisibleMrMaddHatter
Dementia praecox
 User Gallery

Registered: 06/07/02
Posts: 1,418
Loc: Nowhere
Re: Honey Water Cloning [Re: GratefulDread]
    #2315947 - 02/08/04 11:32 PM (12 years, 9 months ago)

Sounds good to me.
Liquid culture kicks ass  :grin:


--------------------
I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.
Hunter S. Thompson

It's always tea-time, and we've no time to wash the things between whiles.

Don't be a fool, this is a war on drugs. Act like. The government sure does!


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OfflineGratefulDread
Never pass up anopportunity topee.

Registered: 10/21/02
Posts: 366
Last seen: 12 years, 2 months
Re: Honey Water Cloning [Re: MrMaddHatter]
    #2317453 - 02/09/04 01:02 PM (12 years, 9 months ago)

I'll get some pics of the jars up as soon as I can get this damn am working again.


--------------------
Before you criticize someone, you should walk a mile in their shoes.
That way, when you criticize them, you're a mile away and you have their shoes.


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OfflineKush44
Stranger
Registered: 09/04/09
Posts: 44
Last seen: 6 years, 10 months
Re: Honey Water Clonin [Re: GratefulDread]
    #11578586 - 12/03/09 07:49 PM (7 years, 6 days ago)

i really want to use this tek but there is one thing confusing me. What does it mean to macerate with knife ? Does is mean cut into small pieces ? i really need to know to do this i looked in dictionary and it doesnt mean what i thought it meant


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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Agar, Herb Grinder, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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