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Invisiblegugglebum
newbie
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Registered: 01/02/04
Posts: 54
Pretty basic question about innoculating...
    #2314946 - 02/08/04 04:07 PM (20 years, 1 month ago)

Hi, I was wondering if it's a big problem if the syringe touches your verm/BRF substrate during the innoculation - the way I take it from the PF tek (www.fungifun.org/pf) is that you're just supposed to shoot it against the glass and let the drop go down ... right?
Thanks!

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Offlinevermonster
Lost Sailor

Registered: 10/17/03
Posts: 207
Loc: Shangri La
Last seen: 10 years, 5 months
Re: Pretty basic question about innoculating... [Re: gugglebum]
    #2314961 - 02/08/04 04:12 PM (20 years, 1 month ago)

Stab it right in there alongside the glass, shoot and then
go to the next hole. You can't avoid getting substrate on
the needle.


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Invisiblegugglebum
newbie
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Registered: 01/02/04
Posts: 54
Re: Pretty basic question about innoculating... [Re: vermonster]
    #2315009 - 02/08/04 04:34 PM (20 years, 1 month ago)

OK, so a little flame sterilizing in between each diff. jars wouldn't hurt, right?
Thanks a lot, I just wanted to know if getting some verm on the needle could be a fatal error or whatever ...

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Offlinevermonster
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Registered: 10/17/03
Posts: 207
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Last seen: 10 years, 5 months
Re: Pretty basic question about innoculating... [Re: gugglebum]
    #2315054 - 02/08/04 04:55 PM (20 years, 1 month ago)

Actually my cousin has a small glovebox so she flames once
at the start then sets the syringe in a small jar of alcohol between
jars.


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OfflineBen_Stein
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Registered: 08/15/03
Posts: 370
Loc: Hooterville
Last seen: 19 years, 7 months
Re: Pretty basic question about innoculating... [Re: gugglebum]
    #2315525 - 02/08/04 07:51 PM (20 years, 1 month ago)

Flaming between each jar is a good idea. Cool with alcohol soaked paper towel or Clorox wipes. Verm and substrate are gonna get on the needle, but won't burn on if you cool it.

Also, remember the spores will not like being sizzled thru a flaming hot needle.


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I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames

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Offlinemockeylock
head
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Registered: 02/07/04
Posts: 1,046
Last seen: 13 years, 8 months
Re: Pretty basic question about innoculating... [Re: Ben_Stein]
    #2315720 - 02/08/04 08:39 PM (20 years, 1 month ago)

If the spore syringe needle comes sealed then you don't even need to flame it the first time you use it. I don't flame between jars either. just work quickly in a clean environment and you won't have any problems.


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OfflineBen_Stein
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Registered: 08/15/03
Posts: 370
Loc: Hooterville
Last seen: 19 years, 7 months
Re: Pretty basic question about innoculating... [Re: mockeylock]
    #2316173 - 02/08/04 10:23 PM (20 years, 1 month ago)

The idea here revolves around never really knowing where contams are coming from, and how clean your environment truly is.

If you should happen to pick up a contam, which could even be in or on one of the jars, then you will have great success spreading it to every other jar.

I've gotten away with plenty of ridiculously unclean practices, but for a noob question such as this, I think the answer should be:
Yes, flaming is a good habit to get into. It takes a few seconds and will eliminate many questions in the long run.


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I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames

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