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OfflineFrommer
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Agar vs PDA
    #23139868 - 04/21/16 10:02 AM (8 years, 8 months ago)

Hi guys

What I want to know is when I isolate Myc. when do I use Agar or PDA.
I have been trying to get a strain onto PDA to isolate it.
But the problem I am having is its not taking on PDA.

The spores contam quick, The LC I used didn't even contam it holds no life.
So I have taken some Myc off a straw tek Im running and placed that on PDA.

Ive started to wonder if I missed something and maybe I must use strait agar no dextrose added.

Can anyone clear up for me the difference between the two, or point out something obvious Im missing?

The way I get it onto the Agar/PDA is not important for me, I just want to get it going so I can isolate good culture for future teks.

I have read a few teks and the only thing I see that dose not seem 100% is PDA vs Agar.


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OfflineBobabouy
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Re: Agar vs PDA [Re: Frommer]
    #23139878 - 04/21/16 10:05 AM (8 years, 8 months ago)

PDA is Agar. Potato Dextrose Agar. No, don't use Agar by itself. Do a search and learn to make PDA or MEA (Malt Extract Agar).

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Offlinelukehighwalker710
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Re: Agar vs PDA [Re: Bobabouy]
    #23139885 - 04/21/16 10:07 AM (8 years, 8 months ago)



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:vaped: Highwalker :bongload:

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OfflineYerow
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Re: Agar vs PDA [Re: Frommer]
    #23139898 - 04/21/16 10:11 AM (8 years, 8 months ago)

There are probably 100's of agar recipies, the thing is that you want to change your mixtures once in a while, some months use PDYA, other months use Grain water agar, other times Malt extract agar, and so on

If your culture wether it be in the form of agar/syringe wont colonize on your plates, or simply growing contamns you are more than likely making them too dry/too wet or have a strange mixture! might also just be your culture  :shrug:

So.. not really any definite answer to your question

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Offlinemolemole
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Re: Agar vs PDA [Re: Yerow]
    #23140107 - 04/21/16 11:18 AM (8 years, 8 months ago)

As far as the spores not germinating, like yerow said the plate is probably to dry. Try making a batch of plates with a little more water. Not much like 2-3 ml more depending on how big of  a batch u are doing.
Could also be sterile technique and there is too much contamination.


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InvisiblebodhisattaMDiscordReddit
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Re: Agar vs PDA [Re: molemole]
    #23142737 - 04/21/16 11:02 PM (8 years, 8 months ago)

agar link in my signature

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OfflineFrommer
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Re: Agar vs PDA [Re: bodhisatta]
    #23187467 - 05/04/16 04:08 AM (8 years, 8 months ago)

Thanks guys sorry late response.


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InvisibleJosex
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Re: Agar vs PDA [Re: lukehighwalker710]
    #23187691 - 05/04/16 07:03 AM (8 years, 8 months ago)

Quote:

lukehighwalker710 said:
Here's a great tek by a TC and mod https://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1





Do this ^^^, it's easy AF and very beginner-friendly.

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Offlinemushpunx
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Re: Agar vs PDA [Re: Josex]
    #23187935 - 05/04/16 08:42 AM (8 years, 8 months ago)

My go to recipes are
10g potato flake, 10g agar, teaspoon honey or karo syrup, 500ml water
Or
10g , 10g malt extract, 500ml water

I rotate between MEA and PDA when I pour sleeves.

I adjust the nutrients or agar stiffness as needed


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Amateur Mycologists United
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