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Offlinemushpunx
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Re: Question about directly printing spores on agar [Re: Psilicon] * 1
    #23144375 - 04/22/16 01:44 PM (8 years, 8 months ago)

Dude your photos are too huge for me to see

I like to print onto sterile foil or right onto a sterile disposable pitre dish.. its actially pretty nice I just keep the plates wrapped up with parafilm

I flame sterilize my loop, cool it in the agar in the receiving plate, and rub a tiny section of the print with the loop. The agar on the loop helps pick up the spores. Then I swipe the loop across the plate in a zig zag pattern


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OfflinePsilicon
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Re: Question about directly printing spores on agar [Re: mushpunx]
    #23144388 - 04/22/16 01:50 PM (8 years, 8 months ago)

The printing on foil and drying is important because it kills any non-endospore-forming bacteria which were on the cap (lots and lots).


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Agar - what, why and how?  Everything a beginner needs to know.
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OfflinePsilicon
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Re: Question about directly printing spores on agar [Re: Psilicon]
    #23153790 - 04/25/16 02:46 PM (8 years, 8 months ago)

So how's it going with this?


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Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Offlinemushpunx
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Re: Question about directly printing spores on agar [Re: Psilicon]
    #23154760 - 04/25/16 06:53 PM (8 years, 8 months ago)

Quote:

van der griegen said:
The printing on foil and drying is important because it kills any non-endospore-forming bacteria which were on the cap (lots and lots).





Are you saying its a bad idea to print on sterile pitre (without agar in it) instead of foil?


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Offlinespacechildo
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Re: Question about directly printing spores on agar [Re: mushpunx]
    #23154774 - 04/25/16 06:58 PM (8 years, 8 months ago)

pretty sure he just means the drying process, not so much what the print is on.

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Offlinemushpunx
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Re: Question about directly printing spores on agar [Re: spacechildo]
    #23155422 - 04/25/16 10:31 PM (8 years, 8 months ago)

Quote:

spacechildo said:
pretty sure he just means the drying process, not so much what the print is on.




Ah I gotcha. I always let my prints dry at least 24hrs after printing. Didnt know it killed bacteria!


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OfflinePsilicon
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Re: Question about directly printing spores on agar [Re: mushpunx]
    #23155821 - 04/26/16 02:49 AM (8 years, 8 months ago)

Yeah, it's just the drying part that's helpful, not the aluminum.  Gold, silver and copper are all very bactericidal, but aluminum in a solid state not so much. Printing onto a sterile plate is fine, as long as there's no agar in it (but it's a pretty poor choice financially, considering the cost of a small square of sterile foil).  If there is agar, and if the mushroom touches that agar, all kinds of bacteria will get onto it.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Offlineseilgu
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Re: Question about directly printing spores on agar [Re: Psilicon]
    #23155866 - 04/26/16 03:32 AM (8 years, 8 months ago)

Update:

I'll use external links to show the image this time.
This is taken today 2016.4.26

https://scontent-tpe1-1.xx.fbcdn.net/hphotos-xpf1/l/t31.0-8/13047815_10209672687961990_6461963046681322089_o.jpg

I swiped the spores onto a new plate and after a while they germinated, the spores generated some brown spots I'm not sure what it is,
but there are some white mycelium starting to grow from the edges.
(There are also contaminations so I'm doing a transfer to new plates)

It looks OK to me but it just grows very slow.


I agree that spore printing to agar is a poor choice, I was trying to directly clone the tissue on agar in the wild, and after cutting the stem, I decided
to put the cap on agar just as a backup. All the stem cuttings failed because of bacteria, but the spores released from the cap succeeded. I was probably lucky.

However, I also tried to print to aluminum foil but that also resulted in bacterial contamination probably because of poor technique.

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InvisibleInocuole
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Re: Question about directly printing spores on agar [Re: seilgu]
    #23156537 - 04/26/16 09:50 AM (8 years, 8 months ago)

Quote:

seilgu said:
Update:

I'll use external links to show the image this time.





Then prepare for no one to click your link...  Uploaded to facebook.. lol.


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OfflineTheDeathryderIsBac
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Re: Question about directly printing spores on agar [Re: Inocuole]
    #23158265 - 04/26/16 06:06 PM (8 years, 8 months ago)

I'm not sure wtf that brown crap is.  Does look funky though.

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