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seilgu
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Question about directly printing spores on agar
#23135814 - 04/19/16 11:17 PM (8 years, 8 months ago) |
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So I placed a mushroom cap on agar and now have a relatively clean spore print without contamination, on MEA agar.

Now I'm not sure how to deal with it, because the usual method is to print on paper and scrap off into a jar or syringe.
Questions : 1. Will the spores grow into mycelium on the petri dish it's printed? Because the spore density is so high, maybe they will signal each other not to germinate?
2. Does the agar plate hold enough water for the spores to germinate, or should I add a few drops of sterile water on the spore print?
3. Does the spores need different "sexes" to reproduce? Do I need to collect a different mushroom spore print to mate with it?
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lukehighwalker710
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Re: Question about directly printing spores on agar [Re: seilgu]
#23135821 - 04/19/16 11:19 PM (8 years, 8 months ago) |
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Haha odd! Following..
3. I'd say that's a definite no.
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HamHead
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Re: Question about directly printing spores on agar [Re: seilgu]
#23135827 - 04/19/16 11:22 PM (8 years, 8 months ago) |
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They may or may not germinate.
Yes, agar hold enough water for spores to germinate. Though it is common practice to put a minimum amount of spores on agar.
No, the spores don't need a different sex. They're not plants and don't require pollen or anything of the sort.
Interested to see if you get any growth.
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TheDeathryderIsBac
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Re: Question about directly printing spores on agar [Re: HamHead]
#23135911 - 04/20/16 12:17 AM (8 years, 8 months ago) |
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Look at the first link in my signature. Early on I explain the lifecycle of a mushroom and how it reproduces. Basically spores germinate as monokaryotic mycelium, then when 2 chains of compatible monokaryotic mycelium meet, they combine into dikaryotic mycelium, which is type of mycelium mushroom fruitbodies are constructed from.
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Psilicon
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Re: Question about directly printing spores on agar [Re: seilgu]
#23136039 - 04/20/16 01:18 AM (8 years, 8 months ago) |
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Quote:
seilgu said: So I placed a mushroom cap on agar and now have a relatively clean spore print without contamination, on MEA agar.
Sorry, but no, you don't. You have a really nasty agar plate that you should just throw away now.
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seilgu
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Re: Question about directly printing spores on agar [Re: Psilicon]
#23136150 - 04/20/16 02:26 AM (8 years, 8 months ago) |
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I've already cut wedges and moved them on to new plates, so those might be completely sterile. We'll see in a few days.
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Chk
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Re: Question about directly printing spores on agar [Re: Psilicon]
#23136231 - 04/20/16 04:14 AM (8 years, 8 months ago) |
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if he did things correctly from an invitro fruit, i don't see much problems here.
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blindingleaf
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Re: Question about directly printing spores on agar [Re: Chk]
#23136253 - 04/20/16 04:31 AM (8 years, 8 months ago) |
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why aren't the spores black?
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Chk
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Re: Question about directly printing spores on agar [Re: blindingleaf]
#23136254 - 04/20/16 04:32 AM (8 years, 8 months ago) |
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Since he already transfered a wedge, i guess the picture show germinated spores?
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Inocuole
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Re: Question about directly printing spores on agar [Re: blindingleaf]
#23136280 - 04/20/16 05:03 AM (8 years, 8 months ago) |
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Quote:
blindingleaf said: why aren't the spores black?
https://www.shroomery.org/forums/showflat.php/Number/23135729
He wild-printed to agar.
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blindingleaf
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Re: Question about directly printing spores on agar [Re: Inocuole]
#23136310 - 04/20/16 05:32 AM (8 years, 8 months ago) |
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oh i thought it was a cube.
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
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mupetmower
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Re: Question about directly printing spores on agar [Re: blindingleaf]
#23136372 - 04/20/16 06:11 AM (8 years, 8 months ago) |
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this istn gonna work, is it? gonna be dirty af, and isnt that just way too many spores? you just need a few, which are microscopic.. oh well, ill be watching to see what happens haha.
i have never heard of printing straight onto an agar plate? is this a thing?
-------------------- -The wise man never stops seeking knowledge.
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Psilicon
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Re: Question about directly printing spores on agar [Re: mupetmower]
#23136642 - 04/20/16 08:45 AM (8 years, 8 months ago) |
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Quote:
mupetmower said: this istn gonna work, is it? gonna be dirty af, and isnt that just way too many spores? you just need a few, which are microscopic.. oh well, ill be watching to see what happens haha.
i have never heard of printing straight onto an agar plate? is this a thing?
No, that isn't a thing. Like you said, it's way too much inoculant, even if it's from a sterile fruit--and these aren't.
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seilgu
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Re: Question about directly printing spores on agar [Re: Psilicon]
#23143807 - 04/22/16 09:57 AM (8 years, 8 months ago) |
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Update :
I think I got some spore germination.

Sorry for the bad quality of the picture. After I took it I made a transfer to another clean plate, the mycelium doesn't look very solid tho. But I don't think it's contamination.
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twistedty
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Re: Question about directly printing spores on agar [Re: seilgu]
#23143810 - 04/22/16 09:58 AM (8 years, 8 months ago) |
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did you take a pic with a microscope
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mupetmower
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Re: Question about directly printing spores on agar [Re: twistedty]
#23143826 - 04/22/16 10:01 AM (8 years, 8 months ago) |
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-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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seilgu
Stranger

Registered: 08/02/11
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Last seen: 6 years, 8 months
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Re: Question about directly printing spores on agar [Re: twistedty]
#23143840 - 04/22/16 10:06 AM (8 years, 8 months ago) |
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Yes that's taken from a microscope, with a camera looking into the eye piece...

Without microscope, it looks like this from the bottom side of the petri dish.
Edited by seilgu (04/22/16 10:24 AM)
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twistedty
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Re: Question about directly printing spores on agar [Re: seilgu]
#23143844 - 04/22/16 10:07 AM (8 years, 8 months ago) |
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Quote:
seilgu said: Yes that's taken from a microscope, with a camera looking into the eye piece...
Well i like it.
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Josex
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Re: Question about directly printing spores on agar [Re: seilgu]
#23143911 - 04/22/16 10:23 AM (8 years, 8 months ago) |
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Quote:
seilgu said: So I placed a mushroom cap on agar and now have a relatively clean spore print without contamination, on MEA agar.

Now I'm not sure how to deal with it, because the usual method is to print on paper and scrap off into a jar or syringe.
Questions : 1. Will the spores grow into mycelium on the petri dish it's printed? Because the spore density is so high, maybe they will signal each other not to germinate?
2. Does the agar plate hold enough water for the spores to germinate, or should I add a few drops of sterile water on the spore print?
3. Does the spores need different "sexes" to reproduce? Do I need to collect a different mushroom spore print to mate with it?
Please, tell me he's just trolling, didn't read the thread.
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Psilicon
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Re: Question about directly printing spores on agar [Re: Josex]
#23144268 - 04/22/16 12:57 PM (8 years, 8 months ago) |
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Nope, he's serious.
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