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OfflineKizzle
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Recognizing and dealing with contamination * 71
    #23130868 - 04/18/16 04:01 PM (5 years, 6 months ago)

The following guide is unfinished. Feel free to offer information, high quality pictures or to correct any misinformation. Plenty of guides like this have been posted in the past. The difference here is this one will focus on Cubensis in particular although most of the information could apply to any mushroom species being grown.

Signs of Contamination


Sectoring - When two different fungal species are growing in the same substrate they tend to form distinct borders between the two mycelia. Often a line of metabolites may be seen where the two fungi meet. Hourglass shaped mold sectors in PF jars are almost always the result of a compromised dry vermiculite layer whereas circular mold colonies are usually the result of a mold growing from one of the inoculation points.




When the contaminant is bacterial the contaminated area appears uncolonized and are often rectangular shaped. When the mycelium encounters the infected area the edge loses it's thread-like appearance and metabolites are often present.



Sporophores - Sporophores are the spore producing structures of a fungus. In some cases they're large enough to see individually without magnification. When the spores mature most change color making it obvious they're present. However, with a good eye they can be identified before that occurs. The appearance of the larger sporophores before maturation typically resembles a small whisker with a white dot on the end.



Odor - Odor can be extremely helpful in detecting a contaminant when it's not visible. It can also be helpful in telling mushroom mycelium from molds that resemble mushroom mycelium. It is sometimes possible to catch a hidden mold in a spawn jar simply by smelling the spawn before spawning. Odors are easiest to detect immediately after shaking.

Odor can also be helpful when it comes to agar work. While not all molds produce an odor I haven't found a P. Cubensis culture that didn't smell like mushrooms.

Slime - A common sign of bacteria is a slimy appearance of mycelium or grains. In areas where substrate presses against glass and condensation is present brown-yellow slimy rings are often present around the grains. On the other grains it may be noticed as a sort of gel or a crust on the grain's surface. Oils and starches from burst grains may appear similar and but will already be present at the end of sterilization whereas bacteria will appear later.



Dusty texture - As already mentioned sporophores are usually too small to see individually. However they can often be seen collectively as a powdery layer covering the substrate or mycelium. Looking for this texture is very helpful in distinguishing them from bruising or other discolorations the mushroom mycelium may develop.



Discolorations - These are the most obvious signs. Molds often change colors once they've started to produce spores as the spores mature. Some also change the color of the substrate through the production of metabolites. Bruising does not occur in still colonizing spawn or PF jars with exception of after a g2g when using a master jar that has been allowed reach the pinning stage (regardless of whether or not any hyphal knots have actually formed).



There are also discolorations not caused by contamination, see "Not Signs of Contamination" below.

Overlay - Bright white mycelium that colonizes over a colonized substrate, casing layer or the vermiculite fruiting PF cakes have been rolled in is often the first sign of Trichoderma. Watch for the formation of green spores on this area.



Soft patches - Contaminated substrate may become squishy or soft and crumble apart easily. The bright white patches Trichoderma can produce has the consistency of a squishy skin.

Pinning in partially colonized spawn or PF jars - In a properly prepared PF or spawn jar this is contamination related. Unfortunately since the short half-pints have become difficult to find the tall jars are often used in the PF tek in which case that alone can cause pinning before full colonization. It's even common when pints are used for it so consider all that first if no other signs are present.

Spongy mycelium - Molds, especially pins molds, can create a very dense mycelium in jars that seems to press against the glass and fill up the empty volume of the jar. The mycelium has a smooth texture compared to mushroom mycelium.




Not Signs of Contamination



Bruising - Bruising occurs when cell walls in mycelia/mushroom tissue are damaged. Most often this is result of touching, particularly while harvesting the mushroom, and can also occur from dehydration. Dehydration bruising is often widespread in the mycelium and occurs mostly around the base of pins and mushroom on that substrate. Bruising may be green or blue and very heavy bruising may appear black. Extreme bruising is normally found on the stumps of harvested mushrooms.



Spores - Spores produced by Cubensis are a dark violet with the exception of certain mutant/novelty strains. Spores often first appear on the torn veil but if left to sporulate mushrooms will drop large amounts of spores on the substrate and other mushrooms. Air currents may carry spores and deposit them on the tops of the caps.


"Mutant" Blobs - Blobs usually appear when pinning first begin with less blobs being produced later. Mutant blobs are the result of genetic abnormalities and are particularly common in degenerated cultures and certain varieties such as Penis Envy. Environmental conditions also seem to play a role in blob development. On PF cakes they are mostly seen in when cakes are fruited immediately after full colonization.


Rhizomorphs - Rhizomorphs are large threads of mycelium that carry nutrients to pins hence they occur when pinning has begun. Very large rhizomorphs may occur when nutrients are being transferred to a pin growing on a non-nutritious surface such as a glass jar or the sides of a plastic tub. Aerial rhizomorphs are usually seen when a casing layer is used. They appear as cottony strands poking out of the casing layer many of which eventually knot and grow into pins.


Metabolites - Although a large amount of metabolites often indicates contamination, small amounts are common on fully colonized substrates and spawn/PF jars. Metabolites are normally yellow but red metabolites sometimes occur in spawn jars. Metabolites may also appear as a yelllow discoloration of the mycelium.



Aborts - Pins that stop growing, develop a black cap, but are not slimy or show other signs of disease do not seem to be caused by pathogens.



In vitro pinning - Tan growths pressing against the glass, usually developing a dark circle in the center and eventually forming into obvious pins.





Identifying Contaminants



Penicillium
Odors: Musty, Dirt
Sources: Soil, Food, Compost, Air

The most common mold found in indoor air, Penicillium species produce tiny spores which are easily dispersed and stay airborne for relatively long periods of time. Early growth is white and nearly indistinguishable from mushroom mycelium. Colonies are usually circular with a white edge. Most species sporulate very soon after first appearance producing green, yellow, or most commonly blue-green spores. This contaminant is common on agar and in spawn jars where it's ability to spread quickly allows it to overwhelm any mushroom mycelium that may be growing. Contamination after spawning is uncommon and is usually limited to uncolonized grains with little effect on yield. Wood trays can harbor this mold and should never be used in home cultivation.



Known contaminant species include:
P. expansum
P. chrysogenum

Aspergillus
Odors: Musty, Oily
Sources: Soil, Wood, Dust, Air

Also very common indoor air, like Penicillium, Aspergillus species produce tiny spores which can travel relatively large distances before settling. Many species which are common contaminants of mushroom substrates. Mycelium is usually light grey with linear threadlike growth and may be mistaken for mushroom mycelium. Some colonies may appear ringlike with denser mycelium near the edges. Aspergillus produces it's spores on large sporophores which change color as they mature. Color and size of sporophores varies by species and substrate with yellow, black, green, blue and grey all being common, making it easy to mistake with other mold species. Aspergillus is a common contaminant of grain spawn and can be recognized by it's very grainy appearance as spore production begins.



Known contaminant species include:
A. flavus, a yellow to green mold, can produce large amounts of toxins
A. niger, dark brown pins on a white-yellow mycelium
A. fumigatus, blue/grey to green/grey, highly pathogenic to people with suppressed immune systems
A. versicolor, produces a wide a variety of colors

Trichoderma
Sources: Soil, Dust, Clothing
Odors: Coconut, Musty

Although the spores are far less common indoors than the previous molds Trichoderma's aggressive nature makes it one of the most common contaminants in mushroom cultivation. It's mycelium is a tranparent to light grey color and can be very difficult to see depending on the substrate. The first visible sign is often a thick bright white aerial mycelium that grows over the surface of the substrate. Spore are produced on this mycelium turning it a yellow to green color often with a noticeable bright white 'apron' still surrounding the colony. The wet spores it produces are too heavy to become airborne on their own but are able adhere to airborne dust particles and become airborne that way. The sticky nature of the spores make them a common contaminant in still air box work as they easily stick to gloves and jars. Sporulation occurs from various triggers including light, nutrients in the substrate, full colonization, and damage to colonies. Often times sporulation will not be triggered until after spawning, leaving unsuspecting cultivators unaware their healthy appearing spawn is actually contaminated. Smell testing jars before use is essential for catching this mold before spawning although not all species produce a distinct odor.

Contamination is also common after spawning, Freshly spawned grains are quite vulnerable when Trichoderma spores are present in large amounts and cross contamination can occur after handling sources of the spores such as dust and soil. The spores are killed very easily by pasteurization however sterilizing many bulk substrate will actually leave them more vulnerable. Generally Trichoderma does not grow aggressively until it has reached the grain spawn so contamination related to improper pasteurization tends to occur in later flushes.

Casing layers are also vulnerable to Trichoderma. Casing material should always be pasteurized. Although very few species can actually grow on living mushrooms, the enzymes produced can degrade the quality of the mushrooms causing brown spotting and aborts. In case where mold is present on a mushroom will be obvious dark brown damage to the mushroom. In the case of Cubensis it will generally be on old aborted mushrooms. Trichoderma is generally harmless aside allergic reaction to airborne spores so no need to get overly worried about if a mushroom may have hidden mold in it or anything like that. If the mushroom looks healthy keep it. It should be noted however that Trichoderma infected mushrooms may produce the appearance of mold after harvest if not promptly dried and is most likely the case when Trich appears on any harvested mushroom.



Known contaminant species include:
T. viride, more common in bulk substrates, produces a coconut-like odor
T. harzianum, more common on casing layer and as a pathogen of fruit bodies, produces a more typical musty mold odor
T. koningi, no odor, fast spore germination

Mucor
Sources: Soil, Plants, Air

Often referred to as pin mold for the grey to black sporophores which look like tiny pinheads. It's appearance is very similar to Rhizopus.
Commonly a contaminant of spawn and less common in bulk substrates. It typically won't colonize coir/vermiculite substrate but the sporophore may grow to the surface directly from the contaminated spawn below.

Rhizopus
Sources: Soil, Air
Odors: Alcohol, Sour

The fastest growing of the common contaminants. It's appearance is very similar to that of Mucor. It is also capable to parasitizing damaged or aborted mushrooms. It often produces large volumes of aerial mycelium.



Known contaminant species include:
R. stolonifer, grows very rapidly
R. oryzae

Fusarium
Sources: Soil, Plants, Unsterilized Grain, Humidifiers
This mold has a white mycelium very similar to mushroom mycelium and produces bright colors commonly purple, pink, orange, and yellow. Color change commonly occurs after 3 weeks of growth and may not be seen on short-lived agar cultures. Fusarium may inhibit mushroom growth causing mushrooms to remain small with tiny caps and a brown discoloration inside the stem. This can occur even when the mold itself is not visible.

Fusarium contamination is most commonly seen in PF jars as a result of contaminated syringes. The mold looks like mushroom mycelium and grows at a similar speed. After a couple weeks, the jar being about 1/2 - 3/4 colonized the purplish color develops. The spores are generally not airborne but can stick to airborne dust and water droplets.






Cladosporium
Odors: Musty
A relatively common contaminant mold of spawn. Cladosporium's most distinguishing feature is dark-green spores which turn grey or black with age.

Alternaria
Odors: Musty
Usually black or dark grey. Common on agar and spawn and spreads though airborne spores.

Chaetomium
A green mold which can be recognized by the appearance of numerous small green to tan bur like structure spread across the substrate during the spawn run. In many cases mushroom mycelium may colonize into these areas eventually. Chaetomium spore are very heat resistant (for a fungus) and can survive short pasteurization times and/or low pasteurization temperatures. For the most part you'll run into this this in straw or compost as a result of it's preparation and pasteurization.

Monilia
A powdery white, grey, or pink mold.

One form (Neurospora) produces a rapidly growing aerial mycelium which eventually turns bright red or orange.


Neurospora on casing layer

Scopulariopsis
Forms patches of white mold with a powdery appearance. Color may turn slightly pink after a week. It occurs in substrates with a high pH or high ammonia content. Yield is reduce significantly and in worst cases no mushrooms develop at all.

Coprinus
Odors: Various

Coprinus are the most common mushroom producing contaminant. They're appearance indicates there is excess ammonia in the substrate, usually manure, which doesn't support the growth of normal mushroom mycelium. They typically appear during the spawn run and will continue to appear until the ammonia is used up at which point the substrate will become habitable to the normal mushroom mycelium. There are many species so the appearance will vary but they're fairly easy to recognize.

Effect on yield is usually minor. Coprinus fruit bodies should be removed when spotted however since they grow poorly if it all in properly prepared substrate they pose little risk to other substrates.




Schizophyllum
Schizophyllum commune is a mushroom producing contaminant. Indoor occurrences are usually the result of contaminated inoculant.


Diehliomyces
Odors: Chlorine

A pathogen that infects the mushroom mycelium. Mycelium is initially white. Brown lumpy fruit bodies 3-44mm in size appear on the substrate which eventually appear wrinkled and then crumble into powder. It can be prevented with proper pasteurization. Mushroom mycelium may eventually disappear from the substrate.

Bacillus
Odors: Feet, Rotten Apple

Bacillus, the most common contaminant species being Bacillus subtilis, is a bacteria and a common contaminant of spawn producing a foul smell and brownish slime or crust on the grains. Appearance of the bacteria may indicate your sterilization was insufficient and it's occurance is more likely when spawn is incubated at temperatures above room temperature.

It can also be introduced in a contaminated culture or spores. If the spawn hasn't been shaken since inoculation this is indicated by it's appearance starting on the upper grains and spreading downward. The most common contaminant species are non-motile and generally do not spread quickly in spawn unless shaken but may originate on multiple grains when sterilization related.



Known contaminant species include:
B. subtilis, the most heat resistant species
B. cereus

Yeasts
Odors: Various

There are many yeasts you might tun into in spawn or on agar. Although many people assume their appearance will resemble baker's yeast (which is dehydrated), colonies actually appear very similar to bacteria. Some yeasts are easily recognized by tiny spots, usually white or yellow, appearing through the grain jar. Other yeasts are indistinguishable from bacteria. Their presence slows the mycelium's colonization or stops it completely. They are not contaminants of bulk substrates nevertheless when present in the spawn they are capable of stalling growth after spawning.


Pseudomonas
Odors: Rot, Fruity
Sources: Lime, Peat moss

Pseudomonas contains many bacteria which are harmless or even beneficial to the fruit bodies and small amounts are naturally present on the mushroom surface. Problems arise when water is in contact with the fruit body for prolonged periods of time, usually from condensation, misting, or soaking. Large amounts of the bacteria can produce enough enzymes to degrade the cell walls of the mushroom. Symptoms are often superficial with minor brown spotting that do not affect the taste, texture, or odor of the mushroom. If the wet conditions persist they can progress to larger brown slimy patches and grooves and can even lead to degradation of large parts of the cap.

Since the bacteria is naturally present, prevention depends on providing adequate air exchange to allow moisture droplets to evaporate quickly. Slimy pins should be removed, especially before misting since the splash can spread large amounts of the bacteria which accelerates the process on the other fruit bodies.




Known contaminant species include:
P. tolaasii, primary agent of bacterial blotching

Cladobotryum
Sources: Soil, Plants
Odors: Rot, Dirt

This is a parasitic fungus that grows on mushrooms and over casing layers. There are many species and symptoms vary depending on species of the mold and the mushroom. It causes what is known as cobweb disease which is characterized by a cobweb mycelium covering the mushroom leading to decay of the fruit body and heavy spore production. Spotting is particularly common when spores are present in the air. Growth on the casing layer and heavy infection usually occurs in later flushes. In all cases no signs of the mold appears before pinning has initiated. The mold is strictly a casing layer and fruit body contaminant, and does not prevent colonization of spawn or bulk substrate nor are they sources of the infection.



Known contaminant species include:
C. dendroides, AKA Dactylium dendroides
C. mycophylum, produces a dirt-like odor

Verticillium
Sources: Soil, Plants, Flies

A parasitic mold occurring on all mushroom species the effects of which can vary greatly in severity and form. The most common symptom is brown spotting on the caps and stems. Unlike bacterial blotch the spotting is dry, usually indented, and in very high humidy a light grey fuzz forms in the center. The fungus often infects a single side of a mushroom causing that area to stop growing. As the healthy tissue continues to grow it can cause peeling of stem and lesions and cracking in the cap which may tilt to one side. When primordia become infected the result is small "bubbles" of infected mushroom tissue. Infected tissue on both the bubbles and on growing mushrooms is brown to grey with a dry leathery appearance. Yields are reduced mainly from degradation in mushroom quality and the total weight of the mushrooms grown is not greatly affected.

The cause of this disease is exposure of the developing fruit bodies to verticillium spores. Symptoms appear 7-14 days after the exposure. Unlike competitor molds the spores do not grow significantly on the substrate and effects are generally only noticed on the mushrooms themselves. Spores must come in direct contact with part of a fruit body and will then cause symptoms in that area. Spawn in not normally a vector of the contamination and the bulk substrate is only a possible vector when a casing layer is not used. Pasteurization will destroy any verticillium spores in a substrate or casing material but good hygiene is needed to prevent recontamination.

The primary source of the spores is soil. Spores are sticky and do not become airborne easily from simple air movement. They can be spread indoors though airborne dust, insects, on hands and clothes, and most importantly by the aerosols created by the water splash while misting. A single spray of water hitting a contaminated mushroom or surface can launch spores up to two feet away.



Known contaminant species include:
V. fungicola
V. psalliotae

Mycogone
Odors: Rot

Mycogone is a parasitic fungus which infect primordia and small pins causing monstrous deformations. Once infected the primordia develop into a blob-like mass of mushroom tissue, very large in some cases. When pins are infected they develop swollen stripes and deformed small caps. The mold mycelium appears as a white mat colonizing the outside of the mushroom. As the disease progresses brown discolorations form, colored metabolites may ooze from the mushroom, and eventually an unpleasant odor is produced. The mold spreads quickly often infecting most or all fruit bodies present.

Mycogone is most commonly seen when using a casing layer. It may also occur when pinning begins before full colonization and improve after colonization has finished. This is presumably due to the vulnerability of primordia when forming beneath a surface.

Known contaminant species include:
M. perniciosa
M. rosea


Cause of Contamination



Contaminated inoculant
Causes: Mold or bacteria in PF jars
        Mold or bacteria in grain spawn
        Mold or bacteria during or after spawn run (uncommon)

When a spore syringe is contaminated most or all jars inoculated with that syringe usually show the same contaminants. They are common problem for new cultivators. Obtain syringes from a reputable vendor and use peer reviewed techniques for making your own.

When spores transferred from a particular area of a spore print are contaminated the result is usually a single contaminated jar or plate. Any syringes made from that transfer will usually be contaminated. Limit transfers to small amounts of spores only to help avoid this. Avoid taking spores from outer edges of the printed area.

Liquid cultures are easily contaminated as all contaminants in the inoculant used to create them will be directly exposed to the medium (the broth). Contaminated liquid culture jars usually appear normal even when contaminated although heavy bacterial contamination can give it a cloudy appearance or create blob-like formations.

Agar cultures can be contaminated without showing signs. The contaminant could be something that landed on a colonized area or something growing beneath the mycelium. To avoid this isolate mycelium from spore or tissue inoculated plates by transferring a small piece of mycelium from the outer edge of the colony with an inoculation loop. Fully colonized plates should be avoided as the hyphae can grow up and out the sides of the plate. It may be helpful to smell the inoculation after a transfer if you suspect a contaminant may be present.

Botched inoculation
Causes: Mold or bacteria in grain spawn
        Mold or bacteria in PF jars
        Mold during or after spawn run
        Bacteria or stalling during spawn run

When steps have been taken to prevent cross-contamination this will result in a single contaminated jar, and at some or all inoculation points in PF jars. Things that can contaminate an inoculation include: touching a needle, scalpel, or inoculation with something that isn't not sterile, touching the sterile inside part of a jar with your gloves, exposure of sterile items to airborne contaminants, moving unsterile item over sterile areas, or moving unsterile items between the filter and sterile while using a flow hood.

Botched grain to grain transfer
Causes: Mold in spawn jars     
        Slow recolonization or stalling in spawn jars
        Slow colonization during spawn run
        Bacteria or stalling during spawn run
        Mold during or after spawn run (most common)
       
Similar causes to botched inoculations. The contaminant typically does not show up until the jar has been spawned. Contaminated g2gs often lead to a large number of jars becoming contaminated. G2Gs should always be done in a still air box or in front of a flow hood. Jars should be checked for odors before spawning. It's particularly important that jars and lids be disinfected as the vibrations created during the transfer can easily shake loose contaminants into the receiving jars.

Insufficient sterilization
Causes: Bacteria and stalling in spawn jars (most common)
        Bacteria and stalling in PF jars     

When sterilization temperature/times are not adequate bacterial contamination may occur. The bacteria is usually widespread in the substrate appearing days to weeks after the sterilization. It's important that steam fills the entire pressure cooker/pot before you start timing the procedure.

Larger substrates require longer times for the heat penetrate to the center, i.e. a gallon jar requires longer than 4 quart jars even though it's same amount of substrate.

Whole grain jars contain a lot of empty space between the grains which slow down heat penetration and require either a pressure cooker or very long sterilization times.

Compromised dry vermiculite layer
Causes: Mold or bacteria in PF jars (most common)
        Mold on dry vermiculite layer of PF jars
        Mold on PF cakes after birthing

When doing the PF the dry vermiculite layer on top acts as a filter. If it fails before the jar is fully colonized contaminants may start to grow in the uncolonized areas. This a common cause of contamination that first appears away from the inoculation points.

If the layer is shifted while moving the jar it creates an opportunity for the contaminated vermiculite on the surface to reach the sterile substrate. For that reason it's a good idea to have the layer fill the entire of the jar so the lid will help hold it in place. Coarse vermiculite in less effective so fine vermiculite should be used. If the layer becomes damp it will not prevent contaminants from reach the substrate.

Filter failure
Causes: Mold in spawn jars
        Bacteria or stalling in spawn jars       

When a filter becomes damaged, wet, or leaves any kind of open gap around the air holes

Improper pasteurization
Causes: Mold in later flushes (most common)
        Mold during spawn run or early flushes

Pasteurization temperatures range from 130-170F. The lower end of that range is not always sufficient in preventing survival of heat resistant molds.

In contrast, when temperatures are too high or sustained for too long it destroys more of the beneficial bacteria which would normally survive. Manure and compost substrates are most vulnerable to this. A common result from excessive pasteurization temperatures is incomplete colonization of the bulk substrate (early pinning) followed by visible Trichoderma infection.

Cross contamination after pasteurization
Causes: Mold during or after spawn run

When spawning it's important to limit exposure of the spawn grains and pasteurized substrate to contaminants. Hands, clothes, and hair are major vector for recontamination of pasteurized substrate. Cooling substrate should be protected from dust as much as possible.

Casing material is also vulnerable and contaminated casing material may lead to diseases like cobweb or verticillium. If using unpasteurized casing material it should be used straight from freshly opened or sealed bags.

Insufficient gas exchange during spawn run
Causes: Bacteria or stalling during spawn run
        Mold during spawn run
        Fermentation odor during spawn run

A lack a gas exchange while bulk substrates are colonizing creates anaerobic conditions which can stall the recovery and colonization of the mushroom mycelium and promote the growth of anaerobic bacteria. A fermentation odor may develop. In serious cases the mushroom mycelium does not recover allowing mold to colonize the grains.

Insufficient cooling of bulk substrate
Causes: Bacteria or stalling during spawn run
        Mold during spawn run

Mushroom mycelium is easily killed by high temperatures. All of the bulk substrate must be cooled to room temperature before spawning.

- Unfinished

*title edited 3/28/2020


Edited by LogicaL Chaos (03/28/20 07:24 PM)


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InvisiblePastywhyteMFacebook
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Re: Recognizing and dealing with contamination [Re: Kizzle] * 3
    #23130900 - 04/18/16 04:16 PM (5 years, 6 months ago)

Fantastic work Kizzle, stickied :congrats:

I would like to chime in that verticillium fungicola has been reclassified as Lencacillium fungicola to distinguish it from the verticillium species that attack plants.

Very nice work man.


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Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 2
    #23131962 - 04/18/16 08:50 PM (5 years, 6 months ago)

:takingnotes:

Excellent write up Kizzle.


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OfflinemyceliumEX
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Re: Recognizing and dealing with contamination [Re: Grey] * 3
    #23132191 - 04/18/16 09:37 PM (5 years, 6 months ago)

:whathesaid:
Very easy to follow, and great info on how to identify contams.


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Re: Recognizing and dealing with contamination [Re: myceliumEX] * 2
    #23133079 - 04/19/16 02:54 AM (5 years, 6 months ago)

Great post! I've bookmarked it.:thumbup:


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Re: Recognizing and dealing with contamination [Re: jimmyjams] * 2
    #23143527 - 04/22/16 09:41 AM (5 years, 5 months ago)

great post! this is amazing, and ill deff be saving this as a bookmark!


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OfflineGreenPrayingMantis
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Re: Recognizing and dealing with contamination [Re: SpicyWizard] * 2
    #23201691 - 05/08/16 11:38 AM (5 years, 5 months ago)

thanks for the very good guide


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Offlinesauroman1
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Re: Recognizing and dealing with contamination [Re: GreenPrayingMantis] * 2
    #23225531 - 05/14/16 12:49 PM (5 years, 5 months ago)

How to deal with bacteria endospores? I have suspicion that spore prints might be contaminated after repeatedly getting ruined PF cakes.


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Re: Recognizing and dealing with contamination [Re: sauroman1] * 2
    #23225653 - 05/14/16 01:23 PM (5 years, 5 months ago)

Usually endospores are a problem in cereal grain and we deal with them via pressure cooking. If your inoculate is the vector then you either need to get new prints or start working with agar to clean it up. Actually agar is the better road IMO. At that point tho most people get pressure cookers and start with grains.


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Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 2
    #23230050 - 05/15/16 03:33 PM (5 years, 5 months ago)

Yes, agar dish has advantages, but used it only for cyanescens. Endospores can be problem in any nutritious enviroment. Question is what it's vulnerable sides compared to cube spores? Keeping in water for long time can eliminate spores?
Don't have currently access to pressure cooker. But I've read that for Pf cakes it's not prerequisite.


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Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 2
    #23274034 - 05/26/16 03:56 PM (5 years, 4 months ago)

How is the bacteria manifesting? I mean is it showing up right away? Is it localized to the inoculation points or what?


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Re: Recognizing and dealing with contamination [Re: Kizzle] * 2
    #23281741 - 05/28/16 05:47 PM (5 years, 4 months ago)

:thumbup:


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Re: Recognizing and dealing with contamination [Re: PsilocyBen17] * 2
    #23355549 - 06/17/16 07:52 PM (5 years, 4 months ago)

Great guide! Very informative


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Re: Recognizing and dealing with contamination [Re: Sgt. Overkill] * 2
    #23357709 - 06/18/16 01:14 PM (5 years, 4 months ago)

Yes, great post here...kinda answered the wuestion in my post, but hoping for a more specific answer to my problem...great job


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Re: Recognizing and dealing with contamination [Re: funkymonk22] * 2
    #23374176 - 06/23/16 05:34 PM (5 years, 3 months ago)

Any thoughts on this. After first flushhttp:/


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Re: Recognizing and dealing with contamination [Re: fundyfresh] * 1
    #23374252 - 06/23/16 05:56 PM (5 years, 3 months ago)

Trich. At least ya got a flush.


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Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 2
    #23391770 - 06/28/16 07:46 PM (5 years, 3 months ago)

Great post.  This is awesome.        Hey I have a question.  Sorry for the long text.  Wanted to explain quickly.    I was looking for a good place to check and I guess this is as good as any.      Ok so here's what happened.  Two of my cakes were too moist and started to ferment just a little.  I make wine and know the smell well and caught it early.    Mushrooms grow naturally around here and used to outback the neighbor said but someone dug a bunch up and never came back.    So I knew a perfect spot and dumped the cakes out in some shade but where light is perfect.  And hunidty and moisture is great.  I sprayed them down a bit so they would be rinsed and maybe grow hopefully.    I found a chunk of Mycellium growing about 2weeks later and it was under a leaf on a chunk of the cake I missed when rinsing.      I grabbed it up carefully and put it in some pasturized horse poo/straw/coco coir/Verm.  And put the chunk in the middle and layer of coco/Verm On top of it with some dry Verm too. 
I set it in an Aqaurium I had and slid it under my Motorhome so it wouldn't get rained on or in the sun.    Today here's what it looks like.    Is this anything I should keep still?    It really looked like the proper nice white Mycelium but now since its come thru the casing layer it's looking a tad bit different and just wanted a few opinions on wether j should keep or toss


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Re: Recognizing and dealing with contamination [Re: Skillzd] * 2
    #23478319 - 07/26/16 12:39 PM (5 years, 2 months ago)

Is this contamination? Pitching it regardless, other jar has no progress, can post more photos.
Day 13 now for this jar.



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Re: Recognizing and dealing with contamination [Re: Dubtubs420] * 2
    #23568780 - 08/23/16 12:47 PM (5 years, 1 month ago)

Basically anything not white, right?


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Re: Recognizing and dealing with contamination [Re: nakano] * 2
    #23575511 - 08/25/16 11:49 AM (5 years, 1 month ago)

Good Morning, I was hoping that someone would be able to help me. I'm in my first flush and pining has started, however I have, what looks like some contamination occurring, on half of the surface,it looks like green mold, I tried to scoop it all out, but it just keeps coming back, should I salt that whole area, and hope for the best, or do I just let it go for now. Any help from someone with more knowledge then me would be greatly appreciated. Looking forward to hearing from someone. Thank-you


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Re: Recognizing and dealing with contamination [Re: Shaker77]
    #23577721 - 08/25/16 11:17 PM (5 years, 1 month ago)

If it's green,  it'll likely keep coming back. I would toss it or bury outside. 

If you're dead set on getting fruits,  and it's still growing them,  maybe don't keep it around your workspace/grows.


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Re: Recognizing and dealing with contamination [Re: Grey]
    #23609729 - 09/04/16 01:02 AM (5 years, 1 month ago)

Hello individual people, and the One Cosmic Universe; same thing.

I have encountered the ghostly green gunk, and it was not pleasant. That smothering smug smell, which permeates it putrid spores over all that which is holy! The madness must cease, if I wish to find peace for me and my mushroom. So this brave knight woketh early when the cock crowed, and boomed "the cleansing will commence in this humble abode!"

So yea, woke up early, went to lowes, acquired equipment, and cleaned the ever living fuck out of my room. 10:1 H20/bleach sprayed the walls, containers, ceiling, everything. Moved 90% of stuff to basement, and kept the essentials in a single container. Cleaned the carpet, sprayed the carpet, ejaculated, but later cleaned up, on the carpet. Fucking hospitals ain't got shit on how sterile my room and closet is bruh. It better be since I spent 12 hours on that ass. No joke. Which makes me realize, now, several failed attempts later, why we need to listen to the experience 'old timers'. Growing is serious business. We will definitely get to play, but, we must first work diligently all the way through, before we blast off. We should treat it like we're building an actual rocket, to help us grow our metaphorical. Every nut and bolt is accounted for before ignition, and inspected multiple times. There are millions of dollars, and crew's lives at stake, so we must take every precaution. That is the way we should approach it; period.

I am going to vanquish thy enemy! No trich bitch is going to ruin my fun!


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Re: Recognizing and dealing with contamination [Re: SilentMajority420]
    #23609766 - 09/04/16 01:20 AM (5 years, 1 month ago)

You're kinda going about it wrong. If you're having issues with trich, cleaning your room isn't the answer. Working on clean spawn and clean agar inoculant is. You only need to be sterile when opening media. That means in your still air box or in front of a hood. Clean rooms have nothing to do with still air box work


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Re: Recognizing and dealing with contamination [Re: Mad Season]
    #23611395 - 09/04/16 03:27 PM (5 years, 1 month ago)

Interesting.

I've been browsing this forum and cultivating for the past few months, and only now am I finding out about this magic 'agar'. It really helps to post and receive responses from experienced users, instead of just watching from the sidelines. Thanks for your help.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #23745426 - 10/17/16 02:16 PM (5 years, 6 days ago)

I'm new here and freaking out about what I have here. Sorry to restart an old post, but this seems the best place.
So in the pictures you can see the colour of the caps are weird, and there are green streaks in the stems. Basically what could cause this, and is it safe to eat?
Sorry again for being a forum noob.



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Re: Recognizing and dealing with contamination [Re: Bhbuda]
    #23745432 - 10/17/16 02:18 PM (5 years, 6 days ago)

Mostly bruising. Looks like there's some insane vert in there. Hella fuzzy feet from no fresh air too, which is what vert thrives in.. is this a kit done in a bag? Those things have the worst conditions for mushrooms. Make a proper fruiting chamber like sgfc.


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Re: Recognizing and dealing with contamination [Re: Mad Season]
    #23745461 - 10/17/16 02:30 PM (5 years, 6 days ago)

Thanks for the fast reply. Yea, they a the tubs in the bags which you only have to flower (I'm lazy). Used them a few times before, but never looked like this, normally I get better fresh air so I don't get the white fuzz so much. This one just went very wrong. If these were yours, would you consume them?

Also, I'm 99% sure they were ment to have brown/golden tops. Is the white heads due to the same infection?


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Re: Recognizing and dealing with contamination [Re: Bhbuda]
    #23745515 - 10/17/16 02:50 PM (5 years, 6 days ago)

Looks like it could just be leucism since they're still sporulating purple spores. There's a few strains which seem prone to this. Like mckenaii, and the obvious AA+

Vert is 100% edible, and almost never gets properly identified, yet nobody goes to hospital from bad medical shit. I'm just saying one of them are exhibiting the dots at the base of the stem, which is a verticillium indicator. I wouldn't print these guys either. Just take better care of them, and grow properly next time :wink: them suckers love air. More than humidity tbh.


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Re: Recognizing and dealing with contamination [Re: Mad Season]
    #23745540 - 10/17/16 02:58 PM (5 years, 6 days ago)

So basically like an albino mushroom? Cool!

These ones are produced by "supa grow" and the type was "Brazil".
Thank you so much, helped me with my stress levels. Sorry once again if I didn't use this section properly.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #23841932 - 11/17/16 04:15 PM (4 years, 10 months ago)

Any idea what this is?



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Re: Recognizing and dealing with contamination [Re: Dlurch314]
    #23841986 - 11/17/16 04:29 PM (4 years, 10 months ago)

Trich


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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #23842059 - 11/17/16 04:51 PM (4 years, 10 months ago)

It's not green though. If it is trich is there anything I can do?


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Re: Recognizing and dealing with contamination [Re: Dlurch314]
    #23842287 - 11/17/16 05:44 PM (4 years, 10 months ago)

Depending on a few factors trich before pins means the spawn was fucked and the whole tub will green up in no time. You can salt trich if the flush is almost done to hold it off for a day or two but this early in the game means it's probably all trash.

You can hang on to it for a day if you want to be sure, it will probably be green in a day and you will know for sure.


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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #23842585 - 11/17/16 07:02 PM (4 years, 10 months ago)

Yeah this one just started pinning a couple of days ago


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OfflineChickie
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Re: Recognizing and dealing with contamination [Re: Dlurch314]
    #23850888 - 11/20/16 11:27 AM (4 years, 10 months ago)

Hello. This is my first grow. Started from a kit with 18 jars and GT spore syringes. Inoc on 11/12. Colonization observed on some jars 3 days in. Today, 8 days after inoc, all jars showing colonization with 2 jars with very minimal signs. All jars have what appear to be healthy mycelium. Notice one jar today with yellowing spots. Can anyone please suggest whether this is contaminate or water damage? My understanding is water damage appears yellow. Also, the area that is yellow appears wet...wetter then rest of jar, if you will. Please see pic. I have isolated the suspect jar from incubator for now. Thanks for your help.



Edited by Chickie (11/20/16 09:41 PM)


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Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #23852515 - 11/20/16 09:23 PM (4 years, 10 months ago)

Moved over a fruiting jar (dunked it) a few days ago and it seems kinda fuzzy today.  I looked through all of the contaminant images and couldn't find any that look like this.  Just wanted to post this and get some thoughts.  The other jars were just moved in today.








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Re: Recognizing and dealing with contamination [Re: Mistylee]
    #23892152 - 12/03/16 11:29 PM (4 years, 10 months ago)

Can anyone please tell me if it is possible for me to save this jar, or should I just toss iht?






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Re: Recognizing and dealing with contamination [Re: psychedethiogenic]
    #23892169 - 12/03/16 11:34 PM (4 years, 10 months ago)

That is completely fucked.


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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #23892769 - 12/04/16 04:53 AM (4 years, 10 months ago)

Quote:

Pastywhyte said:
That is completely fucked.



:punish2::whathesaid: :urreallydumb:  :kidreally:


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Offlinepsychedethiogenic
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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #23893393 - 12/04/16 12:39 PM (4 years, 10 months ago)

Do you happen to know what went wrong? I took extra sterilization caution and kept the jar at around 73 to 75 degrees although I think at one point the temp rose to at least 80 degrees if not more. I also noticed quite a bit of moisture.


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Re: Recognizing and dealing with contamination [Re: psychedethiogenic]
    #23893565 - 12/04/16 01:43 PM (4 years, 10 months ago)

Hard to say. If it's in every jar it's probably a bad syringe or bad inoculation procedure.


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Offlinekrudam2
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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #23939886 - 12/19/16 10:51 AM (4 years, 9 months ago)

thanks for the post.

could we get a picture of metabolites?

I don't know the difference between normal metabolites and infection, and I've had a commercial oyster bag (bought at store) have a large orange bit but customer service told me it was fine and it seemed to grow fine


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OfflineThe Mycologist
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Re: Recognizing and dealing with contamination [Re: psychedethiogenic]
    #23951013 - 12/23/16 10:02 AM (4 years, 9 months ago)

Well whats the deal with that lid?


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Re: Recognizing and dealing with contamination [Re: krudam2]
    #23984419 - 01/05/17 06:31 PM (4 years, 9 months ago)

Quote:

krudam2 said:
thanks for the post.

could we get a picture of metabolites?

I don't know the difference between normal metabolites and infection, and I've had a commercial oyster bag (bought at store) have a large orange bit but customer service told me it was fine and it seemed to grow fine



I added some. Metabolites normally appear in very amounts as in the pics but if the substrate has fully colonized for quite a while or it's been sitting in particularly warm conditions you might see more. If you're seeing large puddles or uncolonized areas then it could still be metabolites your seeing but probably the result of a contaminant.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #23987543 - 01/06/17 09:26 PM (4 years, 9 months ago)

Excellent article. I always send people the link to this thread when they ask questions about contamn.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #23998459 - 01/10/17 04:53 PM (4 years, 9 months ago)

Smells like coconut! Thank you


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Re: Recognizing and dealing with contamination [Re: Jennifer1]
    #24063715 - 02/03/17 11:22 PM (4 years, 8 months ago)

Wow, That was an impressive read. Damn fine job.


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Re: Recognizing and dealing with contamination [Re: Mushenstein]
    #24198792 - 03/28/17 04:48 AM (4 years, 6 months ago)

I'm a total noob at this. This is my first attempt. I had 2 spore syringes and decided to inoculate 3 cakes and jar of liquid per syringe. I sterilized a small room, enclosed it with plastic sheeting, spritzed everything with a bleach solution, used a sterilized glove box, wore a tyvex suit, gloves, a respirator, flame sterilized the needle with an alcohol burner, sterilized the cakes in a pressure cooker @15 p.s.i. for 20 min. and the liquid for 15 to avoid caramalezation and after inoculation everything has been between 70-80 F. Mycelium growth in all six cakes appears fine after 4 days. One of the liquid cultures appears to have no activity while the other has this puffy ball with black spores from the syringe on it. Is this F.U.B.A.R.? Should I inoculate one new cake and see what happens, or should I just pitch it? Thanks.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #24203225 - 03/29/17 07:07 PM (4 years, 6 months ago)

I was also hoping to identify this "stuff" in my one jar... Not to hopeful about it :-(




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Re: Recognizing and dealing with contamination [Re: Aliceinwonderland3]
    #24203243 - 03/29/17 07:15 PM (4 years, 6 months ago)

Quote:

Aquatic Wombat said:




Defintely pitch, try to germinate and clean on agar before trying liquids.

Quote:

Aliceinwonderland3 said:
I was also hoping to identify this "stuff" in my one jar... Not to hopeful about it :-(






Idk it down to the species or even the genus, but it's definitely not clean. Looks like aspergillus maybe.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #24203642 - 03/29/17 09:48 PM (4 years, 6 months ago)

My first Pasty Plate agar try.  I'm guessing this is Penicillium?  It's the only one so far so I'm hoping the others are viable.


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Re: Recognizing and dealing with contamination [Re: Zachorion]
    #24225537 - 04/07/17 02:55 PM (4 years, 6 months ago)

:thumbup: Probs Zach, but cannot be certain from that photo.


Edited by dart21 (04/07/17 02:57 PM)


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Re: Recognizing and dealing with contamination [Re: dart21]
    #24233674 - 04/11/17 01:05 AM (4 years, 6 months ago)



1. Should I be concerned or worried about the cloudier, more solid area in the middle of the plate? I always notice my initial transfer wedge, being higher up than the level plate surface, bubbles up cloudy-like until it flattens out on the receiving plate's horizon. Is that what I'm seeing here?

2. Should the big divide between 11 and 12 concern me?


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OfflineDlurch314
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #24234306 - 04/11/17 11:06 AM (4 years, 6 months ago)

Hey guys in not sure if this is bruising or some kind of contam any ideas?



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InvisibleMad Season
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Re: Recognizing and dealing with contamination [Re: Dlurch314]
    #24237185 - 04/12/17 12:42 PM (4 years, 6 months ago)

Bruising because its hella contaminated with bacteria


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OfflineDlurch314
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Re: Recognizing and dealing with contamination [Re: Mad Season]
    #24237454 - 04/12/17 02:57 PM (4 years, 6 months ago)

So what would you do in my case? Would you pitch it or give it a shot?


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Re: Recognizing and dealing with contamination [Re: Dlurch314]
    #24237630 - 04/12/17 04:05 PM (4 years, 6 months ago)

lol, absolutely get rid of it. Give what a shot? Releasing bacteria into your living area? That's not a shot; it's a guarantee.


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OfflineThe Mycologist
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Re: Recognizing and dealing with contamination [Re: stareatclouds]
    #24237645 - 04/12/17 04:11 PM (4 years, 6 months ago)

Yea i dont like the look of that, transfer away from the cloudiness


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Re: Recognizing and dealing with contamination [Re: stareatclouds]
    #24241042 - 04/13/17 07:17 PM (4 years, 6 months ago)

Already in the dumpster thanks man


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Invisibleoutofservice
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #24470851 - 07/09/17 08:10 PM (4 years, 3 months ago)

Good info, thanks.

After reading this thread I thought these were contaminated, need a second opinion.

They are Pan. Cyan Cambo Sandose.  This is my first grow.
Noc on 5-17, I had two jars start colonizing almost a month after inoc. I've had the rest of the jars sitting in a dark closet waiting for the last two to get to 100%. The closet averages 77-80F, 40-46% humidity.
I've been told the yellow circle is a sclerotia. I hope it is, that would be a cool surprise.

I'm growing these because they're f*cking awesome! A friends friend grew them some time ago and I was hooked. So now I'm trying to grow them. I had my friend look at them and he told me he thought he remembered bluing when his friend grew them.

What are your opinions?  Good or toss?
Anything I can do to save them? Let them sit under a UVC light if needed?

If they're f*cked, is it because I let them sit too long?

I read this in another thread, "Dip them into a sol'n of %10 H2O2, drain them, and return them to their jars."  Is this an option?





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Invisibleoutofservice
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #24470861 - 07/09/17 08:15 PM (4 years, 3 months ago)

Is this Bacillus? If no, what is it?  5 grain, Mexis Jalisco. Trying for stones.



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Invisibleoutofservice
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #24473617 - 07/10/17 09:34 PM (4 years, 3 months ago)

Hey All,

I plan to birth, dunk and roll tmw.

If I could some feedback on my post above with the pics (Pan Cyan Cambo), it would be greatly appreciated.  I don't want to risk contaminating the other jars I have (Mex jalisco and Tampanensis).


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Offlinestewboy350
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Re: Recognizing and dealing with contamination [Re: outofservice]
    #24483771 - 07/14/17 10:51 PM (4 years, 3 months ago)

http://cobweb ?


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Invisibleoutofservice
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Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #24514029 - 07/28/17 03:25 PM (4 years, 2 months ago)

This


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OfflineSteevo
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Re: Recognizing and dealing with contamination [Re: outofservice]
    #24514083 - 07/28/17 03:50 PM (4 years, 2 months ago)

Those grains look a little milky in that second pic. I haven't seen Jalisco colonizing before so it could be fine
Why are you planning on birthing, dunking and rolling that? You can just let it go in the bag for stones :shrug:


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Invisibleoutofservice
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Re: Recognizing and dealing with contamination [Re: Steevo]
    #24514949 - 07/28/17 10:45 PM (4 years, 2 months ago)

Quote:

Steevo said:
Those grains look a little milky in that second pic. I haven't seen Jalisco colonizing before so it could be fine
Why are you planning on birthing, dunking and rolling that? You can just let it go in the bag for stones :shrug:




The bag is for stones. I had a guy who is familiar with the Mexicans say it does colonize in a funky way. Regardless, I'll let it be and ride it out, maybe I'll get some stones.
The jars are what I dunked and cased.


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Re: Stinky cheese smell. [Re: Kizzle]
    #24568191 - 08/20/17 09:54 PM (4 years, 2 months ago)

First of all let me apologize, I am brand new to this site and to cultivating mushrooms. I am finding the site a challenge to navigate. I inoculated two agar plates one week ago and while as far as I can tell the growth on the plates looks normal, they smell strongly of stinky cheese, the mushy, aged kind. I don't know what, if anything, they are supposed to smell like.


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OfflineCrispykoot
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Re: Stinky cheese smell. [Re: margaretlcarlson]
    #24610007 - 09/06/17 11:01 PM (4 years, 1 month ago)

Quote:

margaretlcarlson said:
First of all let me apologize, I am brand new to this site and to cultivating mushrooms. I am finding the site a challenge to navigate. I inoculated two agar plates one week ago and while as far as I can tell the growth on the plates looks normal, they smell strongly of stinky cheese, the mushy, aged kind. I don't know what, if anything, they are supposed to smell like.




Welcome to Shroomery!

Always post pictures if you can...It helps people help you. Don't open your plates up to smell them. That will contaminate them.


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OfflineKrombopulosM
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Re: Recognizing and dealing with contamination [Re: Crispykoot]
    #24652494 - 09/22/17 10:09 PM (4 years, 1 month ago)

Please help me identify if this aerial rhizo is caused by bacteria of some sort




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Re: Recognizing and dealing with contamination [Re: KrombopulosM]
    #24652496 - 09/22/17 10:10 PM (4 years, 1 month ago)

Maybe a little stressed but nothing to fret over. Will probably be fine.


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OfflineKrombopulosM
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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #24654159 - 09/23/17 06:59 PM (4 years, 30 days ago)

thanks, i just made some AA so im gonna transfer to those from my 3rd gen to hopefully get contam free myc for gen 4


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OfflineKoka
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #24670345 - 09/29/17 04:33 PM (4 years, 24 days ago)







[url=https://files.shroomery.org/files/17-39/671678266-20170928_151102.jpg][image]http://www.shroomery.org/forums/thumbs/17-39/671678266-thumb_20170928_151102.jpg[/image[url=http://files.shroomery.org/files/17-39/671677843-20170928_151050.jpg]
Hi guys can anyone tell me are these mushrooms bad or good ? Its my first time growing have no clue. thanks for help


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Offlinepaegan
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Re: [STICKY] Recognizing and dealing with contamination [Re: Koka]
    #24685602 - 10/05/17 12:41 PM (4 years, 18 days ago)



Contam or no contam?


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OfflineChaostoOrder
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Re: Recognizing and dealing with contamination [Re: paegan]
    #24744651 - 10/29/17 04:03 AM (3 years, 11 months ago)

Beautiful Sticky thank you very much!! I believe one needs to study the contaminates just as much, if not more, than the actual species being attempted or identified.  This is very informational thank you!! good job!!!


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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #24816299 - 11/30/17 10:31 AM (3 years, 10 months ago)

Now this here is very helpful and I think everyone needs to read this  a few times especially beginners or anyone who wants to truly understand mycology.
Bookmarked and read a few times, will be passing on the info.
Thank you for this.


Edited by Radagastthebrown (11/30/17 10:33 AM)


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Offlinefukuzi
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #24829305 - 12/06/17 08:11 AM (3 years, 10 months ago)

Hello yellow, some pro outlook is required on these PFtek thingys. They've been around 4 weeks @ 23-28celcius, how do they look like and do they have a bright future? 1 of them shows not much signs of life, but what about these 2 others?

So they 2 are mostly white, but there is some yellowish colors and little bit black. This is a first try, so some pro comments would be really important.

Side A

Side B


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Offlinemushroom_mode
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Re: Recognizing and dealing with contamination [Re: fukuzi]
    #24862473 - 12/22/17 06:50 PM (3 years, 9 months ago)

can a contaminated (unmodified) tub infect a nearby healthy tub if both lids are ajar?

is it worth trying to fruit a tub with a light alcohol odour when the lid is opened?

using coir + oats


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Offlinebeltain
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #25095261 - 03/27/18 06:55 PM (3 years, 6 months ago)

so many ways for it to go wrong! I'm gonna try to grow soon and hope to encounter none of these guys. Thanks for the excellent write-up!


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OfflineTheGreatGonzo
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #25140648 - 04/15/18 11:54 AM (3 years, 6 months ago)

Thanks for the info Kizzle, so much knowledge in one place!


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OfflineJiM aYYYY
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Re: [STICKY] Recognizing and dealing with contamination [Re: TheGreatGonzo]
    #25147891 - 04/18/18 01:36 PM (3 years, 6 months ago)


This is my first tray. I over misted and made some pools on accident overnight. Lesson learned.


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OfflineGroggybandito
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #25170085 - 04/27/18 09:35 PM (3 years, 5 months ago)

Is this contamination, it is in a shotgun furting chamber, 99% R/H, fan 5-6 times a day, been in the chamber for 3 days, brown rice flower verm,


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Re: Recognizing and dealing with contamination [Re: Groggybandito]
    #25195105 - 05/10/18 11:13 AM (3 years, 5 months ago)

what do you think about those , they are contamined or just bruising ?


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OfflineJiM aYYYY
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Re: Recognizing and dealing with contamination [Re: raoulduke948]
    #25195129 - 05/10/18 11:32 AM (3 years, 5 months ago)

Quote:

raoulduke948 said:
what do you think about those , they are contamined or just bruising ?



Contamination for sure. Sorry for the loss man


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Re: Recognizing and dealing with contamination [Re: JiM aYYYY]
    #25195541 - 05/10/18 02:57 PM (3 years, 5 months ago)

I will restart one of them , and one i cleaned , i hope it's gonna be ok , i will come with news soon , cheers ! And thx for support !


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Fantastic [Re: Kizzle]
    #25198690 - 05/12/18 01:54 AM (3 years, 5 months ago)

Amazingly helpful (and unnerving) index, especially useful are the pics of various contaminants. This must have taken months to put together. Fantastic work!

I must admit, though, I'm a little confused by this paragraph:

"Overlay - A common sign of Trichoderma is mycelium that colonizes over a casing layer or the vermiculite fruiting PF cakes have been rolled in. While mycelium can also create overlay, if your fruiting conditions have been adequate to prevent it, this can be one of the first signs you notice."

I'd love some clarification if and when you have the time or inclination.

Again, beautiful work! Thank you!


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OfflineGonzo the Eternal
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Re: Fantastic [Re: SamAtticus]
    #25198817 - 05/12/18 04:17 AM (3 years, 5 months ago)

Great write up! And yes I agree with the guy up top, unnerving as well.


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OfflineJiM aYYYY
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Re: Fantastic [Re: Gonzo the Eternal]
    #25205351 - 05/15/18 01:24 PM (3 years, 5 months ago)


What type of contamination would you call this?


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Re: Recognizing and dealing with contamination [Re: JiM aYYYY]
    #25235804 - 05/29/18 12:15 PM (3 years, 4 months ago)

I found some weird spot in my BRF cake, i don't know can you tell me whats going on with this pictures.. today is 19 day after inoculation (koh samui, ms syringe) and everything looks great, but this spot!.. i can tell for sure nothing is growing there (for now) it looks like a bit wet and maybe missing some brown rice flour, but I'm 80% sure that wasn't there few days ago.. also those in picture that looks like black dots in that area isn't black dots but holes in verm/brf mixture.. also i steam sterilized 9 jars for 2 hours... all doing great... and this is 277ml jar if that's important..






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Re: Fantastic [Re: SamAtticus]
    #25267099 - 06/14/18 04:59 AM (3 years, 4 months ago)

Quote:

SamAtticus said:
Amazingly helpful (and unnerving) index, especially useful are the pics of various contaminants. This must have taken months to put together. Fantastic work!

I must admit, though, I'm a little confused by this paragraph:

"Overlay - A common sign of Trichoderma is mycelium that colonizes over a casing layer or the vermiculite fruiting PF cakes have been rolled in. While mycelium can also create overlay, if your fruiting conditions have been adequate to prevent it, this can be one of the first signs you notice."

I'd love some clarification if and when you have the time or inclination.

Again, beautiful work! Thank you!



Thanks for pointing that out. In part it's confusing because I meant to upload some better pictures later but I also rewrote it so it makes more sense. Basically what I meant is when you notice mycelium growing over parts of the substrate you normally don't see it growing particularly in comparison with identically made substrates. When you have an actual casing layer you normally aim for it to not be colonized, and to mostly have rhizomorphs growing through it but then suddenly there's this patch growing over it and...
:scream:


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Invisiblenube424
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Re: Fantastic [Re: JiM aYYYY]
    #25295857 - 06/27/18 10:45 PM (3 years, 3 months ago)

Quote:

JiM aYYYY said:

What type of contamination would you call this?



Um I think it's contaminated with psilocybe cubensis?


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Offlinetombonytom
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Re: Recognizing and dealing with contamination [Re: nube424]
    #25312278 - 07/06/18 09:18 PM (3 years, 3 months ago)

just made a liquid culture with honey, took my old liquid culture which has proven success and a lot of it.
but I'm quite new to lc, I know it's bad, but why not take the challenge.

my problems are, mycelium doesn't really float, more like dingle to the bottom..
I have kind of frisbee made of mycelium, which floats and looks like something I could pour out of an old bottle of milk..

it came from a clean culture, should I dump?
or just add some absinth, put on my pirate wood leg, chuck it and yell at the neighbors?


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Re: Recognizing and dealing with contamination [Re: tombonytom]
    #25322887 - 07/12/18 09:01 PM (3 years, 3 months ago)

Hey yall,

I did  not want to create another thread since it appears many people have posted on this thread for help in identifying potential contamination. I am currently harvesting some fruit, but I noticed a few spots on a variety of cakes that are giving me some concern. I took some photos, and if I did this right they should load. If you could provide any assistance in letting me know if it could be something, or if I am just being over cautious it would be greatly appreciated!:cool:

Shot 1:


Shot 2:


Shot 3:


Shot 4:


Thanks for any help, and much love to you all!


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OfflineKizzle
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Re: Recognizing and dealing with contamination [Re: BloneDude]
    #25323387 - 07/13/18 03:55 AM (3 years, 3 months ago)

It's just mycelium caused by the higher Co2 levels near the substrate and blue bruising from the substrate drying out.


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Invisiblenube424
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #25323608 - 07/13/18 09:25 AM (3 years, 3 months ago)

What I wanna know is, is everything we call trich actually trich? Or is most of it actually penicillin and we just can't tell the difference?


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OfflineFunGuyZ
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Re: Recognizing and dealing with contamination [Re: nube424]
    #25339310 - 07/21/18 05:39 PM (3 years, 3 months ago)

Is this Trich- bad feeling



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OfflineAnkhSaisRaKhamun
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Re: Recognizing and dealing with contamination [Re: Grey]
    #25374770 - 08/08/18 01:17 PM (3 years, 2 months ago)

what happens when all growth stops and is dry?


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Re: Recognizing and dealing with contamination [Re: AnkhSaisRaKhamun]
    #25374809 - 08/08/18 01:36 PM (3 years, 2 months ago)

Then u throw it out. And start over.


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OfflineIncreduLuck
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Re: Recognizing and dealing with contamination [Re: nube424]
    #25380873 - 08/11/18 05:35 AM (3 years, 2 months ago)

Hey guys, newb cultivator here.. This started and has been growing for like a week on the side of my container.. Do you think it's the aerial rhyzomorphs or something more serious? It's in strands and seem to grow upwards.
Picture:


Edited by IncreduLuck (08/11/18 02:48 PM)


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Invisiblenube424
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Re: Recognizing and dealing with contamination [Re: IncreduLuck]
    #25381370 - 08/11/18 12:17 PM (3 years, 2 months ago)

Upload the pic to the site. The little mountain icon over there when ur posting  ------->>


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OfflineIncreduLuck
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Re: Recognizing and dealing with contamination [Re: nube424]
    #25381577 - 08/11/18 02:49 PM (3 years, 2 months ago)

Alright, I edited my previous post to include it. Thanks for the instructions!


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Re: Recognizing and dealing with contamination [Re: IncreduLuck]
    #25382565 - 08/11/18 11:54 PM (3 years, 2 months ago)

It's just mycelium, and it looks like there are some knots forming nearby :thumbup:


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OfflineIncreduLuck
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #25384831 - 08/13/18 04:21 AM (3 years, 2 months ago)

Thanks for the advice! It's just weird to see mycelium grow up from the medium.


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Re: Fantastic [Re: Kizzle]
    #25421729 - 08/29/18 04:48 PM (3 years, 1 month ago)

Quote:

Kizzle said:
Quote:

SamAtticus said:
Amazingly helpful (and unnerving) index, especially useful are the pics of various contaminants. This must have taken months to put together. Fantastic work!

I must admit, though, I'm a little confused by this paragraph:

"Overlay - A common sign of Trichoderma is mycelium that colonizes over a casing layer or the vermiculite fruiting PF cakes have been rolled in. While mycelium can also create overlay, if your fruiting conditions have been adequate to prevent it, this can be one of the first signs you notice."

I'd love some clarification if and when you have the time or inclination.

Again, beautiful work! Thank you!



Thanks for pointing that out. In part it's confusing because I meant to upload some better pictures later but I also rewrote it so it makes more sense. Basically what I meant is when you notice mycelium growing over parts of the substrate you normally don't see it growing particularly in comparison with identically made substrates. When you have an actual casing layer you normally aim for it to not be colonized, and to mostly have rhizomorphs growing through it but then suddenly there's this patch growing over it and...
:scream:




Thank you so much for the clarification! Much appreciated.


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Looking for verification. [Re: Kizzle]
    #25436984 - 09/05/18 10:26 AM (3 years, 1 month ago)

First time for everything first grow, first flush, new member, and first post.  I am using rye berries in 110 quart mono tubs, with six 2 1/2 inch air exchange holes, three high and three low all stuffed with poly.
Everything has gone well until I put my myc in the mono tub. The first time I watered them I bruised one tub very badly and the other not so much. Thought I had contamination and freaked out. Now thanks to this site, I have learned to mist my tub’s and I have pins popping all over. Oh yes these are golden teachers on day 7 since the first pins appeared. I have a couple with fuzzy feet that I think is from poor fae, but with my poly and I fan them with each misting(up to 5 times/day if I’m home) I want a more experienced opinion. Thanks in advance and Thank You greatly for all the info on this site.


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Re: Looking for verification. [Re: Friedmind]
    #25444361 - 09/08/18 06:41 AM (3 years, 1 month ago)

It's very dry in there. Awesome job for ur first grow though!!!! Next time try making the poly on the bottom a lot tighter to hold the humidity in. If u dial in the tub right u shouldn't have to mist much.

Fuzzy feet are hard to avoid too. And it can happen from misting too. Ur on the right track tho man, I can tell uve been reading for awhile :smile:


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Re: Looking for verification. [Re: nube424]
    #25450519 - 09/10/18 09:06 PM (3 years, 1 month ago)

Thank you. Glad to hear I’m doing something right lol. First flush gave me just over 5oz of dehydrated product between the two tubs. Somewhere I read to pluck em soon as the veil tears, so I ended up with a bunch of little ones. Towards the end of the flush I read that it doesn’t hurt to let them spread their caps, the last few were huge. Any input as to which way is best?


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Re: Looking for verification. [Re: Friedmind]
    #25451603 - 09/11/18 07:57 AM (3 years, 1 month ago)

They say for potency it's best to pick early. And f ur trying to avoid spores all over ur shrooms. Otherwise it doesn't hurt to let them open. If u want max weight without getting spores everywhere, pick the ones with open caps b4 they drop spores everywhere, and leave the rest till they start to open up.


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Re: Looking for verification. [Re: nube424]
    #25478413 - 09/21/18 06:20 PM (3 years, 1 month ago)

At the end of my second flush I had spores drop, now 3 days later there is no new growth except for this (see picture) in one corner. Is my tub done for? Or can I cut out this corner and see what happens?


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Re: Looking for verification. [Re: Friedmind]
    #25478415 - 09/21/18 06:22 PM (3 years, 1 month ago)

Quote:

Friedmind said:
At the end of my second flush I had spores drop, now 3 days later there is no new growth except for this (see picture) in one corner. Is my tub done for? Or can I cut out this corner and see what happens?




No it's done. Put it outside, it's compost now.


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Re: Looking for verification. [Re: Pastywhyte]
    #25478872 - 09/21/18 08:50 PM (3 years, 1 month ago)

Quote:

Pastywhyte said:
Quote:

Friedmind said:
At the end of my second flush I had spores drop, now 3 days later there is no new growth except for this (see picture) in one corner. Is my tub done for? Or can I cut out this corner and see what happens?




No it's done. Put it outside, it's compost now.




:whathesaid:

Caught a case o' Trichoderma


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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #25487094 - 09/25/18 04:29 AM (3 years, 29 days ago)

I have just tried to inoculate some jars with two different spore syringes  on the 20th and I have started to have Cobweb mold in them. Do you think my syringes are bad or what is going on? It seems the cobweb mold starts at about the 3-4 day mark and I don't believe it is the syringes because the mold started on the outside of the cakes and I inoculated them straight down into the substrate as I did not know I was supposed to inoculate towards the glass. What is going on and how can I prevent this? This is my first time doing this and so far it has been very dissapointing, I already have to chuck out 5 jars and I don't want to waste anymore solution or jars.



Edited by orionstarseed (09/25/18 04:31 AM)


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Re: [STICKY] Recognizing and dealing with contamination [Re: orionstarseed]
    #25487400 - 09/25/18 09:32 AM (3 years, 28 days ago)

Sterilize ur jars longer. Cobweb mostly comes from not sterilizing well enough ime.

And yes, next time inoculate toward the glass so the water drips down the glass. It's easier to monitor growth.


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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #25502196 - 10/01/18 01:06 AM (3 years, 23 days ago)

Newbie question:

When I cut my shrooms to dry, I noticed blue-green everywhere I cut. Is this contamination? Is it bruising? Are these safe to eat?


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Re: [STICKY] Recognizing and dealing with contamination [Re: alorac]
    #25502202 - 10/01/18 01:12 AM (3 years, 23 days ago)

Its bruising and safe to eat


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OfflineGris
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Re: [STICKY] Recognizing and dealing with contamination [Re: Wing]
    #25529114 - 10/11/18 10:17 AM (3 years, 12 days ago)

Getting started with agar and this pink dot showed up on one of my plates.

Anybody that could help identify and can I just cut it out of the plate ?





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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #25531024 - 10/11/18 11:57 PM (3 years, 12 days ago)

On the list of non contaminants, you should include primordia. I had a bunch of yellow/brownish blobs form in my jars, and I thought it was some sort of bacterial infection. Come to find out it was primordia that eventually turned into knots and pinned. Probably just a rookie mistake, but since the point of this forum is helping rookies, it might be worth adding. Just a thought!


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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #25540338 - 10/15/18 09:56 PM (3 years, 8 days ago)



what do you think?
is there mold present?
this is after the 2nd surge of mushrooms.
there is a white fuzz growing that is new


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Re: [STICKY] Recognizing and dealing with contamination [Re: mayot02]
    #25541137 - 10/16/18 07:04 AM (3 years, 8 days ago)

That's thrich. It'll be green shortly. Good eye. Most new people don't notice mold till it changes colors.


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Re: [STICKY] Recognizing and dealing with contamination [Re: nube424]
    #25549934 - 10/19/18 12:59 PM (3 years, 4 days ago)



I got a nice flush out of these cakes, but this one had a good bit of blue bruising throughout the whole process. I dunked and rolled in verm for a second flush last night and about 14 hours later, I noticed that this patch where it was very blue is now looking awfully dark and almost gray. I think it's just bruising getting worse on that spot and i'm gonna keep an eye on it. Anyone more experienced think it looks more like a contam?


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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #25642754 - 11/27/18 03:36 PM (2 years, 10 months ago)

Hi Folks,

  There's a long black thing in my grain jar. It's coming out of a particularly dark oat. I don't think it was there before. It looks like some kind of stroma. Admittedly, I haven't paid much attention to this batch, and it could've been there all along.

Also, I know the moisture is too high in these jars. My mistake. Please don't drag me.

Have you seen anything like this before?

Thanks!





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Re: [STICKY] Recognizing and dealing with contamination [Re: whiskeyjack]
    #25642764 - 11/27/18 03:41 PM (2 years, 10 months ago)

Could be ergot or just a rotted grain or was overly ripe when they picked it.


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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #25650686 - 12/01/18 03:24 PM (2 years, 10 months ago)

I love that you used one of my pics in this. :congrats:


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Re: Recognizing and dealing with contamination [Re: Oatman2000]
    #25667330 - 12/09/18 10:15 AM (2 years, 10 months ago)

thank you for this helpful sticky post! i'm always curious what kind of contamination there is.
a few days ago i had these mushrooms

and

when they started looking wet i first thought thats just some water because of my misting and will evaporate. but it didnt and a moderate smell of dirty laundry appeared and i threw them away.
i guess it was some kind of bacteria and my misting-mistake (too much? also on the fruit bodys)?


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Re: Recognizing and dealing with contamination [Re: fusselchen]
    #25667768 - 12/09/18 01:46 PM (2 years, 10 months ago)

Bottom one is just a mutant..normal in MS...The white flecks are also pretty standard, but the wet patches likely bacterial infection, probably over misting initially, usually you'd see it on the stipe too


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Re: Recognizing and dealing with contamination [Re: fusselchen]
    #25675318 - 12/12/18 08:51 PM (2 years, 10 months ago)

Quote:

fusselchen said:
thank you for this helpful sticky post! i'm always curious what kind of contamination there is.
a few days ago i had these mushrooms

and

when they started looking wet i first thought thats just some water because of my misting and will evaporate. but it didnt and a moderate smell of dirty laundry appeared and i threw them away.
i guess it was some kind of bacteria and my misting-mistake (too much? also on the fruit bodys)?



I had a MS grow that almost the entire tub was mutants with odd caps like that. Some of them grew with the cap completely inside out, some with the cap almost upside down, some just like yours is there. It's nothing to worry about.


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Re: Recognizing and dealing with contamination [Re: dmar]
    #25683468 - 12/16/18 06:51 PM (2 years, 10 months ago)

I'm concerned that this might be trich, but due to my abundance of side pins, I'm pretty sure that my surface conditions were dry. So it could also be bruising from being dry. It's in a few places on the surface of this sub, but it's very faint. If it is trich, how long would it take for it to become more distinguishable? How fast does trich move once it's started dropping spores and turning green?


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OfflineDtwiz
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Re: Recognizing and dealing with contamination [Re: dmar]
    #25689066 - 12/19/18 11:23 AM (2 years, 9 months ago)




This is my first time growing. I have four jars, inoculated with Puerto Rican.
One of them is showing two light green patches.
What can this be and what can i do?

Thanks in advance


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Re: Recognizing and dealing with contamination [Re: Dtwiz]
    #25689444 - 12/19/18 02:34 PM (2 years, 9 months ago)

Quote:

Dtwiz said:



This is my first time growing. I have four jars, inoculated with Puerto Rican.
One of them is showing two light green patches.
What can this be and what can i do?

Thanks in advance



It's hard to tell with that picture, but if it's green, it's no good. If it's indeed contaminated, pressure cook it at 15 PSI for 90 minutes before you open it to throw it away. If it's kind of a faint color, wait a few days to be sure it's actually green.


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Re: Recognizing and dealing with contamination [Re: dmar]
    #25689552 - 12/19/18 03:30 PM (2 years, 9 months ago)

Okay, I'm totally frustrated here. Last year I had several successful grows: wbs and coir in monotubs. Life happened and I had to stop growing for a few months.  On 11/16/18 I inoculated a bunch of sterile wbs jars with agar. Everything was looking great.  They fully colonized and I made monotubs spawning the wbs with coco coir. I should've documented the date but I didn't (probably around 11/27). My mushrooms just aren't pinning.  They've been really dry due to the winter air and the only room I have with heat that I can grow in is heated by a wood burning stove.  So I've had to mist a lot and still I'm not seeing much condensation on the tubs like I did before. So now I'm thinking they're contaminated.  I don't know.  Anybody?


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Re: Recognizing and dealing with contamination [Re: Growgrl]
    #25689858 - 12/19/18 06:15 PM (2 years, 9 months ago)

Quote:

Growgrl said:
Okay, I'm totally frustrated here. Last year I had several successful grows: wbs and coir in monotubs. Life happened and I had to stop growing for a few months.  On 11/16/18 I inoculated a bunch of sterile wbs jars with agar. Everything was looking great.  They fully colonized and I made monotubs spawning the wbs with coco coir. I should've documented the date but I didn't (probably around 11/27). My mushrooms just aren't pinning.  They've been really dry due to the winter air and the only room I have with heat that I can grow in is heated by a wood burning stove.  So I've had to mist a lot and still I'm not seeing much condensation on the tubs like I did before. So now I'm thinking they're contaminated.  I don't know.  Anybody?



Looks like bruising from pooling water, up FAE and stop misting for now.

Edit: at second look around the poly looks like really healthy myc, definitely up the fae !


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Edited by XnMe (12/19/18 06:17 PM)


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Re: Recognizing and dealing with contamination [Re: XnMe]
    #25690255 - 12/19/18 09:27 PM (2 years, 9 months ago)

Could be misting too much / too hard also. Just give it a light mist from a few feet above. High pressured mist can bruise mycelium


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Re: Recognizing and dealing with contamination [Re: nube424]
    #25690744 - 12/20/18 06:52 AM (2 years, 9 months ago)

Okay.  I'll up the fae and fewer mists from a higher level. Thanks for the replies.


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Re: Recognizing and dealing with contamination [Re: Growgrl]
    #25690768 - 12/20/18 07:28 AM (2 years, 9 months ago)

Any time chica. When u mist, u want it to look like morning dew on the surface. Just tiny droplets that glisten. U only need to mist if it actually looks dry.


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Re: Recognizing and dealing with contamination [Re: nube424]
    #25693852 - 12/21/18 03:52 PM (2 years, 9 months ago)

Quote:

Okay, I'm totally frustrated here. Last year I had several successful grows: wbs and coir in monotubs. Life happened and I had to stop growing for a few months.  On 11/16/18 I inoculated a bunch of sterile wbs jars with agar. Everything was looking great.  They fully colonized and I made monotubs spawning the wbs with coco coir. I should've documented the date but I didn't (probably around 11/27). My mushrooms just aren't pinning.  They've been really dry due to the winter air and the only room I have with heat that I can grow in is heated by a wood burning stove.  So I've had to mist a lot and still I'm not seeing much condensation on the tubs like I did before. So now I'm thinking they're contaminated.  I don't know.  Anybody?




The morning after I posted this, I woke up to pins! lol


Edited by Growgrl (12/21/18 03:55 PM)


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OfflineOctopus8
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #25835850 - 02/25/19 01:18 AM (2 years, 7 months ago)

This is like the equivalent of sexual education and seeing all the pictures of STD's.

Great work!


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Re: Recognizing and dealing with contamination *DELETED* [Re: Octopus8]
    #25874301 - 03/14/19 07:03 PM (2 years, 7 months ago)

Post deleted by Blacklight98

Reason for deletion: Wasn’t edited


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Re: Recognizing and dealing with contamination [Re: Blacklight98]
    #25874355 - 03/14/19 07:34 PM (2 years, 7 months ago)

Could anyone tell me if this shroom looks okay there is white fuzzies around all the stocks i read where someone was saying its fine if there is white fuzz on the stock said it was just myc because of lack of air exchange but the discoloring at the bottom of the stem right when i Cropped it and i cut it open an it was white inside dont smell bad


Edited by Blacklight98 (03/14/19 07:39 PM)


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Re: Recognizing and dealing with contamination [Re: Blacklight98]
    #25924873 - 04/09/19 01:40 PM (2 years, 6 months ago)

These mycobags were inoculated 3 weeks + 2 days ago and showed ZERO growth until last week. I mashed this bag with a couple of spots only because I thought "whatta heck, I've got nothing to lose anymore," since I was about to toss all the other bags. Out of 5, 2 survived. 1 shows a normal pace and healthy growth.

This one is sweating a lot. It's also colonizing faster (perhaps because of the mash?). I am not sure, because I see healthy myc inside, but also a thinner white veil and this to me seems excessive sweating. Thoughts?



--------------------
In Hebrew, the words "wine" and "secret" hold the same numerologic value. When wine comes in, secrets spill out. Do you think the person who said that knew mushrooms? When mushrooms come in... Is there anything beyond a secret?



Edited by Count of Sabugosa (04/09/19 01:41 PM)


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Re: Recognizing and dealing with contamination [Re: Count of Sabugosa]
    #25931736 - 04/13/19 04:16 AM (2 years, 6 months ago)

It's hard to see what's going on in that bag. I'd give it some time to finish colonizing and check it out then when you can open the bag.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #25931803 - 04/13/19 05:55 AM (2 years, 6 months ago)

Here's 4.5 days after 100% break.



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Re: Recognizing and dealing with contamination [Re: Count of Sabugosa]
    #25934594 - 04/14/19 06:14 PM (2 years, 6 months ago)

I’ve got a really nice pin set, but trich just showed up. What should I do Cut out the trich and let it flush?  Smother the trich in limestone powder?  Toss it in my worm bin and let it flush In There?


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Re: Recognizing and dealing with contamination [Re: Land Trout]
    #25935227 - 04/15/19 12:32 AM (2 years, 6 months ago)

So I already threw this jar away when I saw this,


But, what kind of contamination is this and what would have caused it?

I also have 5 other jars next to this one (like a few inches apart on the same table) when I discovered this particular jar. The other jars do not show anything similar, but would they be at risk for anything since they were so close to the contaminated jar (like maybe spread contamination airborne or something)?


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Re: Recognizing and dealing with contamination [Re: Pusynality]
    #26033231 - 06/04/19 10:33 PM (2 years, 4 months ago)

im guessing black mold? ive had a feew jars look exactly like that. I either get rid of the jar or deff isolate it from the others and out of your grow area


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OfflineMycelial Messenger
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #26038242 - 06/07/19 01:33 PM (2 years, 4 months ago)

Great article


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Re: Recognizing and dealing with contamination [Re: Mycelial Messenger]
    #26073132 - 06/25/19 04:35 PM (2 years, 3 months ago)

Very nice write up I’ll definitely be saving this


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Re: Recognizing and dealing with contamination [Re: Shroomterd]
    #26083857 - 07/01/19 11:36 AM (2 years, 3 months ago)

Seems like all of my grain jars have been contaminated. Trying to identify what with exactly and narrow down where could I have introduced it. It's very fluffy and appeared 4 days after ms injections in places of those injections. Rye was boiled for an hour and then PC'd for another hour. Any guesses?
😶




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Re: [STICKY] Recognizing and dealing with contamination [Re: Zeak]
    #26086596 - 07/02/19 06:31 PM (2 years, 3 months ago)

My first real grow has been going really well until today when I noticed a couple of blue-ish spots in my tub. Do you think it's mold? If so, can I quarantine those spots somehow or should I go ahead and harvest what mushrooms have already grown and call it a bust?





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Re: [STICKY] Recognizing and dealing with contamination [Re: the_mycologist]
    #26109935 - 07/15/19 05:47 PM (2 years, 3 months ago)

So I've been doing many bulk grows with cubes (B+, Treasure Coast, Golden Teachers, Blue Meanie, Koh Samui Super Strain, Pink Buffalo) and had great success on inoculating the jars in a SAB. Only contams I ever had was trich after many flushes of the tubs, so not big losses.

I decided to try out some Pan Cy's in agar dishes, inoculating directly from a syringe. All the plates I did turned to mold within 3 days. This is my first attempt with agar and my SAB was always nuked prior to working in it and I take many precautions, so I'm a bit confused. I used Bods methods for agar, following to a T and still got contams. Is there anything different with Pans? Should I just inoculate jars instead and then attempt to clone my Pans, since I know I don't get contams direct to jar?


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Re: [STICKY] Recognizing and dealing with contamination [Re: FryMe]
    #26129106 - 08/09/19 10:57 PM (2 years, 2 months ago)

Is this contamination?

If so, anything I can do, any suggestions? 

First tub




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Re: Recognizing and dealing with contamination [Re: Zeak]
    #26143012 - 08/19/19 04:41 PM (2 years, 2 months ago)

I have the same problem. And the really bizarre part is, if you let it grow, it sprouts these weird-ass mushroom shaped' things.



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OfflineCOGSx86
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Re: Recognizing and dealing with contamination [Re: SamAtticus]
    #26146496 - 08/21/19 05:05 PM (2 years, 2 months ago)

Quote:

SamAtticus said:
I have the same problem. And the really bizarre part is, if you let it grow, it sprouts these weird-ass mushroom shaped' things.






sane, ate the mushrooms, and Im still alive :wink:


Edited by COGSx86 (08/21/19 05:05 PM)


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Re: Recognizing and dealing with contamination [Re: COGSx86]
    #26150213 - 08/23/19 11:48 PM (2 years, 1 month ago)

Man, a guy just shared this and I have never seen anything like it. What in the holy Hell is this???? Looks like some alien bacterial blobs. I just can't put a name to it.



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Re: Recognizing and dealing with contamination [Re: Spiritualscience]
    #26152935 - 08/25/19 11:06 PM (2 years, 1 month ago)

Does anyone have a name for this?



Here's another:


Edited by peanutbutterwaferb (08/27/19 11:00 AM)


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Offlineontheinternet
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Re: Recognizing and dealing with contamination [Re: peanutbutterwaferb]
    #26165012 - 09/02/19 11:30 AM (2 years, 1 month ago)

Second monotub grow and got some weird growth. It's almost like there are mushrooms heads growing on the mushroom heads. I don't think it's a contaminate, but not sure.




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Re: Recognizing and dealing with contamination [Re: ontheinternet]
    #26165126 - 09/02/19 12:43 PM (2 years, 1 month ago)

Totally normal. It will not hurt anything.

Here is a first flush from a tub. It is on its sixth flush now. Only the first flush had the growths. It could be high humidity, minor response to bacteria, etc. Whatever it is, it has no negative effects on the shrooms.



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Re: Recognizing and dealing with contamination [Re: Spiritualscience]
    #26165128 - 09/02/19 12:46 PM (2 years, 1 month ago)

Quote:

Spiritualscience said:
Man, a guy just shared this and I have never seen anything like it. What in the holy Hell is this???? Looks like some alien bacterial blobs. I just can't put a name to it.






Just looks like normal SW penis envy blobs. They are common on the first flush.


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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #26165157 - 09/02/19 01:07 PM (2 years, 1 month ago)

Dang, I think he chunked it already, but I will let him know for sure. He said he put it outside, but that was over a week ago.

Thanks for letting us know!


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Re: Recognizing and dealing with contamination [Re: Spiritualscience]
    #26165185 - 09/02/19 01:24 PM (2 years, 1 month ago)

That’s a shame. Blobs will get some decent size and are just as potent and good to eat.



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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #26165897 - 09/02/19 09:47 PM (2 years, 1 month ago)

Would it be cause for concern if I’ve noticed a slight fermentation/ earthy/ musty smell coming off my substrate 7 days into the spawn run? Initially I had the lid gasket sealed for 4 days before finding out that was a mistake—opened it up and there was a strong fermented smell, but not sure it wasn’t just from having misted H2O2 over the walls and substrate before sealing it up. Now 7 days into spawn run it seems like everything is okay, still notice a slight smell just sort of earthy/musty—nothing crazy.
At 4 days, I flipped the lid upside down for FAE, sprayed a bit more H2O2 and have since just been keeping a close eye on everything. Does thi sound okay by what I’ve described? Any suggestions on how to proceed? I’m not quite 100% colonized yet, temps around 75-78, humidity seems good. Did I dodge a bullet or Is it too early to tell? Below is a picture—seems okay? Anybody see something I’m missing?



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OfflineKizzle
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Re: Recognizing and dealing with contamination [Re: ReptilianBrain]
    #26169223 - 09/04/19 07:37 PM (2 years, 1 month ago)

Quote:

ReptilianBrain said:
Would it be cause for concern if I’ve noticed a slight fermentation/ earthy/ musty smell coming off my substrate 7 days into the spawn run? Initially I had the lid gasket sealed for 4 days before finding out that was a mistake—opened it up and there was a strong fermented smell, but not sure it wasn’t just from having misted H2O2 over the walls and substrate before sealing it up. Now 7 days into spawn run it seems like everything is okay, still notice a slight smell just sort of earthy/musty—nothing crazy.
At 4 days, I flipped the lid upside down for FAE, sprayed a bit more H2O2 and have since just been keeping a close eye on everything. Does thi sound okay by what I’ve described? Any suggestions on how to proceed? I’m not quite 100% colonized yet, temps around 75-78, humidity seems good. Did I dodge a bullet or Is it too early to tell? Below is a picture—seems okay? Anybody see something I’m missing?





The fermentation odor was related to the lack of gas exchange. It's possible for a substrate to recover fully after fixing a problem like that but no guarantees.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #26171753 - 09/06/19 03:08 AM (2 years, 1 month ago)

hi, latest batch of substrate has that sour smell soon after i spawned it. corn spawn very good and clean, straw boiled for over an hour.
only difference from last set of monos was i tried packing the layers of spawn and straw into clear plastic sacks stood in a empty mono to make large bricks then putting the bags on shelving.

I have read that gas exchange problems can cause smells but these are sour.

is there any cure or shall I dinv it outdoor to make another garden patch?

bricks are about 5kg each and are heavily spawned


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Re: Recognizing and dealing with contamination [Re: drspastic]
    #26191576 - 09/16/19 01:56 PM (2 years, 1 month ago)

Can anyone tell me if this is contaminated? The get lighter in color as they grow


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Re: Recognizing and dealing with contamination [Re: Motomushy]
    #26192379 - 09/16/19 07:11 PM (2 years, 1 month ago)

Quote:

Motomushy said:
Can anyone tell me if this is contaminated? The get lighter in color as they grow





Those are too dry. You need another layer of mp tape over the bottom holes. No contams that I can see.


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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #26192930 - 09/17/19 12:43 AM (2 years, 1 month ago)

Thank you! I sprayed a lil water mist in the tub and they have a blueish hue now... That's normal right?


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #26208652 - 09/25/19 03:31 PM (2 years, 28 days ago)

Hello all :-) Amazing original post, thanks for all the great info!! MUSH APPRECIATED!!

This is my first time posting anything so I'm apologizing in advance if not proper...

I've been having this issue and i'm losing sleep:confused:...

colonized jars look and smell great - when they are spawned to bulk and after consolidation this is what is happening around day 7-10 after fruiting:





(I could try taking better pics if necessary)

My worst fear is that this is ASPERGILLUS - Can anyone confirm what it is?

**I've been retracing steps and thinking that my pasteurization technique is prob faulty - vac bags filled with bulk sub and cooked in water (170 for 2hrs) i deff over filled and over wet bulk probably causing to get too hot on outside and not enough in center ??
*** I've also had the window open (no AC and super hot in here) could this have been a factor?

if not aspergillus ...Could these blocks be saved? Maybe soaking in mild bleach solution??

I tossed out about 10 (2L) blocks last time as soon as I saw yellow (they were just starting to fruit)....so sad ... but noticed that it only affected the top - under liner everything looked and smelled healthy....

now I have 8 new tubs that are affected and hoping I can save if possible

If not able to save - no problem - health and safety first and also the MAIN thing I need to know is What am I doing wrong and how can I avoid this in the future???

Thanks so much for your time :heart:


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Re: Recognizing and dealing with contamination [Re: myc.kind.o.lady]
    #26243011 - 10/10/19 05:30 PM (2 years, 13 days ago)

Got a few ugly Bettys growing. Reading through some prior threads it can be over misting and lack of FAE or a genetic mutation. Can anyone advise the exact cause and solution for future growing knowledge ? Appreciate the knowledge folks.



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Re: Recognizing and dealing with contamination [Re: flyingcap]
    #26289644 - 10/31/19 01:45 PM (1 year, 11 months ago)

Thanks to Kizzle for this fantastic, informative thread.

Can anyone identify the beast or beasties that contaminated this tub?




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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #26349508 - 11/27/19 10:44 PM (1 year, 10 months ago)

The first 4 times I ever tried the pftek method I was successful.  I enjoyed the fruits of my labor.  The next 6 times all ended in fast spreading green contamination.  The cakes were fully colonized and put into sanitized fruiting chamber.  I fanned them daily and used the same care as the first 4 successful batches.  Soon after the contamination overtook all the cakes.      Batch after batch until I wasted 6 spore syringes.  I gave up for a couple of years but am going to try again.    This time using a sterilized rye grain kit. 
Many posters here are very adamant about not using a kit but how bad can these kits be.  What’s worse?  Spending $50 on a sterilized kit or wasting syringe after syringe and not knowing exactly where you went wrong?  Appreciate any constructive advice.


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Re: Recognizing and dealing with contamination [Re: tomcards]
    #26366470 - 12/07/19 10:44 AM (1 year, 10 months ago)

Cobweb in the bottom left corner?


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Re: Recognizing and dealing with contamination [Re: ZombiWurm]
    #26368810 - 12/08/19 12:48 PM (1 year, 10 months ago)

Thank you for this detailed write up! I'm brand new to this forum, apologies if this reply breaks any rules. And sorry to ask what may be the obvious but hoping someone more experienced than me can confirm that this cake is contaminated and should just be trashed? OR is it worth continuing?

This is our first grow and we've been following the PF Tek Easy BRF Cakes found here: https://www.shroomology.org/forums/topic/13173-pf-tek-easy-brf-cakes-mushroom-growing-method/

Started on 11/3 with 8 cakes and already had to dispose of 6 of them because of visible contams and really no white mycelium.

Any guidance is greatly appreciated!

https://imgur.com/bP32ldr



Edited by drewman522 (12/08/19 12:50 PM)


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Re: Recognizing and dealing with contamination [Re: drewman522]
    #26368828 - 12/08/19 01:00 PM (1 year, 10 months ago)

That's the wrong jar for BRF cakes. You won't ever get the cake out of that jar. Anyway it looks fine to me. Stick to wide mouth half pint jars. I've always used the regular mouth half pints but it seems the short ones colonize faster


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Re: Recognizing and dealing with contamination [Re: tomcards]
    #26368935 - 12/08/19 02:05 PM (1 year, 10 months ago)

Quote:

tomcards said:
Thanks to Kizzle for this fantastic, informative thread.

Can anyone identify the beast or beasties that contaminated this tub?







hard too really see there. Even so...who knows :/ I don’t think it’s penicillium. It could be aspergillus, chaetomium, trichoderma, maybe cladosporium...but clad is usually darker looking than what’s in your pic. Sadly there are so many “greens” that can eff shit up it’s hard for most of us to say what’s what with certainty


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Re: Recognizing and dealing with contamination [Re: Psilotyl]
    #26369350 - 12/08/19 06:02 PM (1 year, 10 months ago)

Quote:

Psilotyl said:
Quote:

tomcards said:
Thanks to Kizzle for this fantastic, informative thread.

Can anyone identify the beast or beasties that contaminated this tub?







hard too really see there. Even so...who knows :/ I don’t think it’s penicillium. It could be aspergillus, chaetomium, trichoderma, maybe cladosporium...but clad is usually darker looking than what’s in your pic. Sadly there are so many “greens” that can eff shit up it’s hard for most of us to say what’s what with certainty




Yeah it's really hard to tell. You could culture it but even then without a scope you are stuck with observing how it colonized the agar to determine what it is and how the mycelium grows.


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Re: Recognizing and dealing with contamination [Re: ZombiWurm]
    #26370574 - 12/09/19 11:50 AM (1 year, 10 months ago)

Thanks for the feedback. 

My sterile technique was sorely lacking.  There were probably all manner of nasty beasties in the substrate.  After one flush, I harvested 83.3 cracker-dry grams of Golden Teacher.  I dumped the rest of the substrate.  I kick myself as I fantasize about how much that tub could have yielded.

Since then, I've learned much.  This won't happen again.


Tom


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Re: Recognizing and dealing with contamination [Re: ZombiWurm]
    #26370733 - 12/09/19 01:19 PM (1 year, 10 months ago)

Realized I replied to the wrong post, but thanks for the reply! Wow so you think no concerns with contams based on those pics?

Thanks for the note on the jars - these are wide mouth as directed but realized too late exactly what you're saying... may end up having to break them to get the cakes out.


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Re: Recognizing and dealing with contamination [Re: tomcards]
    #26370877 - 12/09/19 02:49 PM (1 year, 10 months ago)

Quote:

tomcards said:
Thanks for the feedback. 

My sterile technique was sorely lacking.  There were probably all manner of nasty beasties in the substrate.  After one flush, I harvested 83.3 cracker-dry grams of Golden Teacher.  I dumped the rest of the substrate.  I kick myself as I fantasize about how much that tub could have yielded.

Since then, I've learned much.  This won't happen again.


Tom




That’s still not bad! There’s always next time, as lame as that sounds lol


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Re: Recognizing and dealing with contamination [Re: Psilotyl]
    #26372091 - 12/10/19 01:54 AM (1 year, 10 months ago)

Is this the meany greeny, or just metabolites?




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Re: Recognizing and dealing with contamination [Re: A_Fun_Guy_Learns]
    #26372913 - 12/10/19 12:58 PM (1 year, 10 months ago)

Quote:

A_Fun_Guy_Learns said:
Is this the meany greeny, or just metabolites?







Looks like that’s probably fine. Keep an eye on it and see if it turns & you’ll get a definitive answer pretty quickly


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Re: Recognizing and dealing with contamination [Re: Psilotyl]
    #26418832 - 01/06/20 09:06 AM (1 year, 9 months ago)

Hi folks, newbie here.
I've just managed my first grow of 5 BRF cakes. I've done one flush so far and have dunked and setup for flush No.2.
Past day or so i've noticed a very slight musky smell coming from my FC which made me head here to try get some info on possible contams.
After reading a lil i am leaning towards a possible Trich contam but im not sure as i obviously lack the experience.
I can still smell the Mycelium as on the first flush but now with a very slight musky smell. I don't see any obvious mold spots but after reading about the white growth that comes before Trich spores i have a feeling this is what i am faced with.
If it wasn't for the slight smell i would have thought these cakes were fine.
Posted some pics so if anyone would like to help out a newbie i'd appreciate the advice. Cheers.







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Re: Recognizing and dealing with contamination [Re: MushToLearn]
    #26419784 - 01/06/20 08:19 PM (1 year, 9 months ago)

All you can do is wait and see.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #26422048 - 01/07/20 11:47 PM (1 year, 9 months ago)

Hi all, thanks to @zombiwurm for replying to my last post. Have some updated photos (and figured out how to include images properly:cool:) so I though i'd post again. These BRF cakes are just over 2 months old and i think looking really good except for a few possible contams in each. Can anyone help identify and is there any cause for concern or should we proceed with the dunk and fruiting?



Thank you!


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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #26453014 - 01/26/20 08:47 AM (1 year, 8 months ago)

After a couple of weeks or so of mushrooms growing on the outside of the casing, however on the top where no mushrooms are growing some green mold has started to appear... any suggestions of what it is or to remedy the situation?? :confused:



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Pink [Re: Kizzle]
    #26456943 - 01/28/20 04:17 PM (1 year, 8 months ago)



Please can anyone help me, does anyone know if my crop is contaminated


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OfflineTynuk
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Re: Pink [Re: D123456]
    #26469563 - 02/05/20 03:26 AM (1 year, 8 months ago)

Hi everyone,
Was hoping if someone could give me an advise. I have 2 spots of dark green mold in my monotub (wbs and coco coir). I hace read on here that I can try to cut them out and cover the spots with salt. Anyone tried doing that before?

Thanks in advance!





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Offlinekillmatrixolme
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #26480287 - 02/11/20 04:14 PM (1 year, 8 months ago)

Can i eat this mushroom?
i think the white color is bold. they are contaminated right?




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OfflineSynKyd
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Re: Recognizing and dealing with contamination [Re: killmatrixolme]
    #26480980 - 02/11/20 10:04 PM (1 year, 8 months ago)

Quote:

killmatrixolme said:
Can i eat this mushroom?
i think the white color is bold. they are contaminated right?







Those are fine.  Scrape the verm off the bottom and enjoy :cheers:


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Offlineendlessnessagain
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Re: Recognizing and dealing with contamination [Re: SynKyd]
    #26483155 - 02/13/20 08:01 AM (1 year, 8 months ago)

Is there anything like this thread but specifically for agar contamination, to help identify them?


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OfflineNigel Stephens
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Re: Recognizing and dealing with contamination [Re: endlessnessagain]
    #26503091 - 02/25/20 02:27 PM (1 year, 7 months ago)

Do I have anything to worry about here, are they safe to eat?
I think it’s pseudomonas or verticillium, stems are hollow too

This is 2nd flush after dunking substrate / floating harvest tek.
Have since removed lid for FAE and drying but should I just harvest and toss? Obvs then bleach my whole grow area :smile:

Thank-you in advance for your time and advice







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OfflineKizzle
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Re: Recognizing and dealing with contamination [Re: Nigel Stephens]
    #26503268 - 02/25/20 04:09 PM (1 year, 7 months ago)

They're still edible. It looks like at an earlier time they were drenched and got waterlogged.


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InvisibleRyeHumor
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2 out of 18 BRF Jars... So long Fare Well? [Re: Kizzle]
    #26503422 - 02/25/20 05:33 PM (1 year, 7 months ago)