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Kizzle
Misanthrope


Registered: 08/30/11
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Recognizing and dealing with contamination 112
#23130868 - 04/18/16 02:01 PM (7 years, 9 months ago) |
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This guide is a work in progress. Feel free to add any contributions, including additional information, high-quality pictures, and corrections to any inaccuracies. While there are many mushroom growing guides available, this one is unique in its focus on Cubensis. However, much of the information presented here can be applied to other mushroom species as well.
Signs of Contamination Sectoring - This refers to the formation of distinct borders between two different fungal species growing in the same substrate. This phenomenon may be accompanied by the appearance of a line of metabolites where the two mycelia meet. In PF jars hourglass shaped mold colonies are typically caused by a compromised dry vermiculite layer, whereas circular mold colonies starting from an inoculation point are usually the result of contaminated inoculatant.     When the contaminant is bacterial the contaminated area appears uncolonized and are often rectangular shaped. When the mycelium encounters the infected area the edge loses it's thread-like appearance and metabolites may be present. Sporophores - Sporophores are the structures of a fungus responsible for producing spores. In some cases, they can be visible to the naked eye without magnification. As the spores mature, most of them change color, making their presence obvious. However, with keen observation, they can be identified before reaching maturity. The early appearance of larger sporophores often resembles a small whisker with a white dot on the end.    Odor - Odor can be highly useful in identifying contaminants when they're not visible. It can also assist in distinguishing between mushroom mycelium and molds that resemble it. In some cases, a hidden mold in a spawn jar can be detected simply by smelling the spawn before use. Odor can also play a helpful role in agar work. Cubensis cultures are known to have a distinct mushroomy odor. This aroma serves as a characteristic identifier during the process, providing a sensory clue to the nature of the culture. Slime - A common indication of bacterial and yeast contamination is the development of a slimy appearance in the mycelium or grains. In areas where the substrate presses against the glass and condensation occurs, you may observe the presence of brown-yellow slimy rings around the grains. It's worth noting that oils and starches from burst grains can have a similar appearance, but they will already be present at the end of the sterilization process. Contaminants, on the other hand, will manifest later on, indicating an issue. Dusty texture - As mentioned before, sporophores are usually too small to be seen individually. However, they often form a collective powdery layer that covers the substrate or mycelium. Paying attention to this texture is crucial for distinguishing sporophores from bruising or other discolorations that can occur in mushroom mycelium. Discolorations - These are the most obvious signs of mold contamination. Molds often change color as they produce spores and some produce metabolites that can alter the color of the substrate.   There are also discolorations not caused by contamination, see "Not Signs of Contamination" below. Overlay - Bright white mycelium that colonizes over a colonized substrate, casing layer or the vermiculite fruiting PF cakes have been rolled in is often the first sign of Trichoderma. Watch for the formation of green spores if these appear.   Soft patches - Contaminated substrate may become squishy or soft and crumble apart easily. The bright white patches Trichoderma can produce has the consistency of a squishy skin. PF cakes may crumble apart easily when contaminated. Pinning in partially colonized spawn or PF jars - In a properly prepared PF or spawn jar this is contamination related. Unfortunately since the short half-pints have become difficult to find the tall jars are often used in the PF tek in which case that alone can cause pinning before full colonization. It's even common when pints are used for it so consider that first if no other signs are present. Spongy mycelium - Molds, especially pins molds, can create a very dense mycelium in jars that seems to press against the glass and fill up the empty volume of the jar. The mycelium has a smooth texture compared to mushroom mycelium.     Not Signs of Contamination Bruising - Bruising occurs when cell walls in mycelia/mushroom tissue are damaged. Most often this is result of touching, particularly while harvesting the mushroom, and can also occur from dehydration. Dehydration bruising is often widespread in the mycelium and occurs mostly around the base of pins and mushroom on that substrate. Bruising may be green or blue and very heavy bruising may appear black. Extreme bruising is normally found on the stumps of harvested mushrooms.   Spores - Spores produced by Cubensis are a dark violet with the exception of certain mutant/novelty strains. Spores often first appear on the torn veil but if left to sporulate mushrooms will drop large amounts of spores on the substrate and other mushrooms. Air currents may carry spores and deposit them on the tops of the caps.  "Mutant" Blobs - Blobs usually appear when pinning first begin with less blobs being produced later. Mutant blobs are the result of genetic abnormalities and are particularly common in degenerated cultures and certain varieties such as Penis Envy. Environmental conditions also seem to play a role in blob development. On PF cakes they are mostly seen in when cakes are fruited immediately after full colonization.  Rhizomorphs - Rhizomorphs are large threads of mycelium that carry nutrients to pins hence they occur when pinning has begun. Very large rhizomorphs may occur when nutrients are being transferred to a pin growing on a non-nutritious surface such as a glass jar or the sides of a plastic tub. Aerial rhizomorphs are usually seen when a casing layer is used. They appear as cottony strands poking out of the casing layer many of which eventually knot and grow into pins. Metabolites - Although a large amount of metabolites often indicates contamination, small amounts are common on fully colonized substrates and spawn/PF jars. Metabolites are normally yellow but red metabolites sometimes occur in spawn jars. Metabolites may also appear as a yelllow discoloration of the mycelium.   Aborts - Pins that stop growing, develop a black cap, but are not slimy or show other signs of disease do not seem to be caused by pathogens. In vitro pinning - Tan growths pressing against the glass, usually developing a dark circle in the center and eventually forming into obvious pins. 
Identifying Contaminants PenicilliumPenicillium is a common mold found in indoor air and is one of the most commonly encountered contaminants in agar and spawn jars. It produces tiny spores that stay airborne for relatively long periods of time. Early growth is white and can be difficult to distinguish from mushroom mycelium. Colonies are typically circular with a white edge. Most species sporulate very soon after first appearance, producing green, yellow, or blue-green spores. This mold contaminant has the ability to spread quickly, overwhelming any mushroom mycelium that may be growing in the same substrate. Contamination after spawning is uncommon and usually has little effect on yield. Penicillium is sourced from soil, food, compost, and air, and has musty or dirt-like odors.     AspergillusAspergillus, like Penicillium, is a commonly found mold in indoor air. It produces tiny spores that have the ability to travel significant distances before settling. Many species of Aspergillus are known contaminants of mushroom substrates, posing a challenge to mushroom cultivation. The mycelium of Aspergillus is typically light grey, exhibiting linear threadlike growth that can be mistaken for mushroom mycelium. Some colonies may appear ringlike, with denser mycelium concentration near the edges. Aspergillus can exhibit different appearances, ranging from sporophores similar to pin molds to a resemblance to Penicillium. The color and size of sporophores vary depending on the species and substrate, with yellow, black, green, blue, and grey being common. This variability can make it difficult to differentiate Aspergillus from other mold species. Aspergillus finds its sources in soil, wood, dust, and the surrounding air. Its odor profile tends to be musty or dirt-like.     TrichodermaTrichoderma, despite being less commonly found as free spores in indoor air compared to other molds, has earned a notorious reputation as one of the most prevalent contaminants in mushroom cultivation due to its aggressive nature. Trichoderma often finds its way indoors through dust particles, which act as common sources and primary carriers of this mold. The mycelium of Trichoderma is usually transparent to light grey, making it quite challenging to spot, depending on the substrate. However, one of the first visible signs of Trichoderma contamination is the development of a thick, bright white aerial mycelium that grows over the surface of the substrate. On this mycelium, spores are produced, giving it a yellow to green color, often surrounded by a noticeable bright white "apron." While the wet spores produced by Trichoderma are too heavy to become airborne on their own within a fresh colony, they can adhere to airborne dust particles and spread through that means. Trichoderma sporulation can be triggered by factors such as light, changes in nutrient availability in the substrate, full colonization, and damage to colonies. Contamination of substrates by Trichoderma typically occurs after spawning. Freshly spawned grains are particularly vulnerable when exposed to a high concentration of Trichoderma spores. Cross-contamination can occur during handling, especially when coming into contact with sources of the spores, such as dust and soil. Although Trichoderma spores are easily killed by pasteurization, sterilizing certain bulk substrates can ironically make them more susceptible to contamination. However, the recovery of spawn grains plays a crucial role as well. Slow recovery, often due to the presence of bacteria in the grains, is a common cause of Trichoderma infection. Anything that slows down the recovery process is a significant risk factor. Infected substrates may display uncolonized patches where the mold's mycelium has taken hold, indicating the presence of Trichoderma mycelium. Visibly growing Trichoderma on living mushrooms is a rare occurrence. In cases where the mold infects a mushroom, obvious dark brown damage or heavy bruising will be noticeable. As a general rule, when harvesting from an infected substrate, if the mushroom appears healthy, it can be kept.    MucorMucor, also known as pin mold, derives its name from the small, grey to black sporophores that resemble tiny pinheads. It bears a striking resemblance to Rhizopus, another common mold. When observed on bulk substrates, Mucor contamination is typically a consequence of contaminated spawn. Mucor originates from soil, plants, and the surrounding air. Keeping a vigilant eye and implementing proper hygiene measures can help prevent the introduction and spread of this mold. RhizopusRhizopus, known for its rapid growth, is one of the swiftest invaders among common contaminants. Its appearance bears a strong resemblance to that of Mucor, making it easy to confuse the two. Rhizopus has the additional ability to parasitize damaged or aborted mushrooms, further complicating matters for mushroom cultivators. One notable characteristic of Rhizopus is its tendency to produce substantial amounts of aerial mycelium. This mycelium, often visible as a cottony or fluffy growth, can rapidly spread across the surface of bulk substrates. Sources of Rhizopus include soil and dust.    FusariumFusarium is a mold that originates from various sources such as soil, plants, unsterilized grain, and humidifiers. It exhibits a white mycelium that closely resembles mushroom mycelium and has the potential to produce vibrant colors such as purple, pink, orange, and yellow. However, it's important to note that color changes may not be evident in short-lived agar cultures. Fusarium contamination has most commonly been seen as a result of contaminated spore syringes. The mold looks like mushroom mycelium and grows at a similar speed. It can take two weeks before the colors develops.  CladosporiumCladosporium, a frequent contaminant mold in spawn, exhibits distinctive characteristics that set it apart. One of its most noticeable features is the presence of dark-green spores, which tend to change in color as they age, transitioning from green to grey or black. This mold carries a musty odor, providing a helpful clue for its identification. It is commonly encountered in spawn during mushroom cultivation processes. AlternariaAlternaria, a common mold found on agar and spawn, is characterized by its typically black or dark grey appearance. It emits a musty odor, which can aid in its identification. ChaetomiumChaetomium is a green mold that can be identified by the presence of numerous small green to tan bur-like structures scattered across the substrate during the spawn run. In some instances, mushroom mycelium may colonize these areas over time. One notable characteristic of Chaetomium is the high heat resistance of its spores compared to other fungi. This means that they can withstand relatively short pasteurization times or low pasteurization temperatures. As a result, Chaetomium contamination is more commonly encountered in straw or compost that has undergone insufficient pasteurization. MoniliaMonilia is a mold that typically appears as a powdery growth in shades of white, grey, or pink. It can be encountered in various environments, including mushroom cultivation settings. One particular form of Monilia, known as Neurospora, exhibits rapid growth with the development of an aerial mycelium. Over time, this mycelium undergoes a color change, turning bright red or orange, providing a distinct visual cue for identification.  Neurospora on casing layer ScopulariopsisScopulariopsis is a mold that manifests as patches of white mold with a powdery appearance. Over time, the color of the mold may transition to a slight pinkish hue, especially after approximately a week. This type of mold tends to thrive in substrates with high pH levels or high ammonia content. When Scopulariopsis contamination occurs, it can have a significant negative impact on mushroom cultivation. In severe cases, it can lead to a complete absence of mushroom development, greatly reducing the overall yield. CoprinusCoprinus is a common mushroom-producing contaminant frequently encountered in mushroom cultivation. Its presence is an indicator of excess ammonia in the substrate, particularly in manure-based substrates. This high ammonia content inhibits the growth of normal mushroom mycelium, leading to the proliferation of Coprinus. These contaminants typically appear during the spawn run phase and continue to emerge until the ammonia levels are depleted. Once the substrate becomes more suitable for normal mushroom mycelium, it may eventually colonize the substrate and replace the Coprinus growth. There are various species of Coprinus, resulting in variations in their appearance. However, they are generally recognizable and distinguishable from Cubensis. SchizophyllumSchizophyllum commune is a mushroom-producing contaminant. It can thrive in various substrates and environments, and its growth can adversely affect the desired mushroom species being cultivated. Its presence indoors is typically attributed to contaminated inoculant or spawn used in the cultivation process. BacillusBacillus, with Bacillus subtilis being the most common species, is a bacterial contaminant frequently encountered in spawn. It is characterized by its distinctive odors, often described as resembling feet or rotten apples. When Bacillus contaminates the spawn, it manifests as a foul smell and the presence of brownish slime or crust on the grains. The appearance of Bacillus bacteria is a sign that the sterilization process may have been insufficient. Additionally, Bacillus can be introduced through contaminated cultures or spores. To prevent Bacillus contamination, it is crucial to ensure proper sterilization protocols are followed and maintained. Adequate drying of the spawn grains help minimize the risk of Bacillus growth.   YeastsYeasts are a diverse group of microorganisms that can be encountered in mushroom cultivation, both in spawn and on agar. Yeast colonies actually resemble bacteria in their visual characteristics. Some yeasts are easily recognizable by the presence of tiny spots, typically white, pink, or yellow, that appear throughout the grain jar. However, there are other yeasts that are indistinguishable from bacteria based on visual appearance alone. Yeast contamination is prevented with good sterile technique. PseudomonasPseudomonas is a genus of bacteria that contains many species, some of which can be found in mushroom cultivation. While certain strains of Pseudomonas are harmless or even beneficial to fruit bodies, problems can arise when there is prolonged contact between water and the mushroom surface. This often occurs due to condensation, misting, or excessive soaking. When present in large amounts, Pseudomonas bacteria can produce enzymes that degrade the cell walls of the mushrooms. The symptoms of Pseudomonas contamination are typically superficial, manifesting as minor brown spotting on the mushroom surface. These spots generally do not affect the taste, texture, or odor of the mushroom. However, if the wet conditions persist, the contamination can progress to larger brown slimy patches and grooves, and may even lead to degradation of significant portions of the mushroom cap. Since Pseudomonas bacteria are naturally present, prevention relies on providing adequate air exchange to facilitate the quick evaporation of moisture droplets. It is important to remove infected pins before misting, as the splashing can spread large amounts of the bacteria and accelerate the contamination process on other fruit bodies.   CladobotryumCladobotryum is a genus of parasitic fungus that commonly infects mushrooms and casing layers in mushroom cultivation. It is typically sourced from soil and plants. There are multiple species of Cladobotryum, and the symptoms can vary depending on the specific species of mold and the type of mushroom being grown. Cladobotryum infection leads to a condition known as cobweb disease. It is characterized by the growth of cobweb-like mycelium that covers the surface of the mushroom, resulting in the decay of the fruit body and heavy spore production. Spore spotting is particularly common when spores are present in the air. The growth and infection of Cladobotryum on the casing layer of mushrooms typically occur in later flushes, rather than during the initial pinning stage. It is important to understand that Cladobotryum is primarily a casing layer and fruit body contaminant. It does not hinder the colonization of spawn or bulk substrate, nor does it serve as a source of infection for them. However, once the mushrooms have formed and the casing layer is in place, Cladobotryum can proliferate and cause significant damage to the fruit bodies. The disease quickly spreads the substrate will stop producing healthy mushrooms and need to be discarded. You should avoid disturbing the contaminated substrate while indoors to prevent spores from being released into the indoor air.   VerticilliumVerticillium is a parasitic mold that can infect various mushroom species. It has multiple sources, including soil, plants, and flies. The severity and manifestation of Verticillium contamination can vary significantly. The most common symptom of Verticillium infection is the appearance of brown spotting on the caps and stems of mushrooms. Unlike bacterial blotch, the spotting caused by Verticillium is dry, typically indented, and may develop a light gray fuzz in the center under high humidity conditions. The fungus often infects a specific side of a mushroom, causing growth to cease in that area. As healthy tissue continues to grow, it can lead to stem peeling, lesions, and cracking in the cap, which may result in a tilted appearance. When primordia (early stages of mushroom development) become infected, small "bubbles" of infected mushroom tissue may form. The infected tissue on both the bubbles and the growing mushrooms appears brown to gray and has a dry, leathery appearance. Verticillium infection occurs when developing fruit bodies are exposed to Verticillium spores. Symptoms typically appear 7-14 days after exposure. Spawn is not typically a vector of contamination. Proper pasteurization of the substrate or casing material can destroy Verticillium spores, but maintaining good hygiene practices is essential to prevent recontamination. The primary source of Verticillium spores is soil. Exposure can occur through airborne dust, insects, contact with contaminated surfaces (e.g., hands and clothes), and most notably, through aerosols created during misting. Even a single spray of water hitting a contaminated mushroom or surface can launch Verticillium spores up to two feet away. Despite it's reputation, Verticillium infection is not common with indoor Cubensis and symptoms caused by bacteria are often mistaken for it.  MycogoneMycogone is a parasitic fungus that infects primordia (early stages of mushroom development) and small pins, resulting in monstrous deformations and rotten smelling fruitbodies. Like Verticillium this one has a big reputation but there's little evidence of it infecting Cubensis. Nevertheless strange growths of unknown causes are often blamed on it. Cause of Contamination Contaminated inoculantCauses: Mold or bacteria in PF jars Mold or bacteria in grain spawn Mold or bacteria during or after spawn run (uncommon) When a spore syringe is contaminated most or all jars inoculated with that syringe usually show the same contaminants. They are common problem for new cultivators. Obtain syringes from a reputable vendor and use peer reviewed techniques for making your own. When spores transferred from a particular area of a spore print are contaminated the result is usually a single contaminated jar or plate. Any syringes made from that transfer will usually be contaminated. Limit transfers to small amounts of spores only to help avoid this. Avoid taking spores from outer edges of the printed area. Liquid cultures are easily contaminated as all contaminants in the inoculant used to create them will be directly exposed to the medium (the broth). Contaminated liquid culture jars usually appear normal even when contaminated although heavy bacterial contamination can give it a cloudy appearance or create blob-like formations. Agar cultures can be contaminated without showing signs. The contaminant could be something that landed on a colonized area or something growing beneath the mycelium. To avoid this isolate mycelium from spore or tissue inoculated plates by transferring a small piece of mycelium from the outer edge of the colony with an inoculation loop. Fully colonized plates should be avoided as the hyphae can grow up and out the sides of the plate. It may be helpful to smell the inoculation after a transfer if you suspect a contaminant may be present. Botched inoculationCauses: Mold or bacteria in grain spawn Mold or bacteria in PF jars Mold during or after spawn run Bacteria or stalling during spawn run When steps have been taken to prevent cross-contamination this will result in a single contaminated jar, and at some or all inoculation points in PF jars. Things that can contaminate an inoculation include: touching a needle, scalpel, or inoculation with something that isn't not sterile, touching the sterile inside part of a jar with your gloves, exposure of sterile items to airborne contaminants, moving unsterile item over sterile areas, or moving unsterile items between the filter and sterile while using a flow hood. Botched grain to grain transferCauses: Mold in spawn jars Slow recolonization or stalling in spawn jars Slow colonization during spawn run Bacteria or stalling during spawn run Mold during or after spawn run (most common) Similar causes to botched inoculations. The contaminant typically does not show up until the jar has been spawned. Contaminated g2gs often lead to a large number of jars becoming contaminated. G2Gs should always be done in a still air box or in front of a flow hood. Jars should be checked for odors before spawning. It's particularly important that jars and lids be disinfected as the vibrations created during the transfer can easily shake loose contaminants into the receiving jars. Insufficient sterilizationCauses: Bacteria and stalling in spawn jars (most common) Bacteria and stalling in PF jars When sterilization temperature/times are not adequate bacterial contamination may occur. The bacteria is usually widespread in the substrate appearing days to weeks after the sterilization. It's important that steam fills the entire pressure cooker/pot before you start timing the procedure. Larger substrates require longer times for the heat penetrate to the center, i.e. a gallon jar requires longer than 4 quart jars even though it's same amount of substrate. Whole grain jars contain a lot of empty space between the grains which slow down heat penetration and require either a pressure cooker or very long sterilization times. Compromised dry vermiculite layerCauses: Mold or bacteria in PF jars (most common) Mold on dry vermiculite layer of PF jars Mold on PF cakes after birthing When doing the PF the dry vermiculite layer on top acts as a filter. If it fails before the jar is fully colonized contaminants may start to grow in the uncolonized areas. This a common cause of contamination that first appears away from the inoculation points. If the layer is shifted while moving the jar it creates an opportunity for the contaminated vermiculite on the surface to reach the sterile substrate. For that reason it's a good idea to have the layer fill the entire of the jar so the lid will help hold it in place. Coarse vermiculite in less effective so fine vermiculite should be used. If the layer becomes damp it will not prevent contaminants from reach the substrate. Filter failureCauses: Mold in spawn jars Bacteria or stalling in spawn jars When a filter becomes damaged, wet, or leaves any kind of open gap around the air holes Improper pasteurizationCauses: Mold in later flushes (most common) Mold during spawn run or early flushes Pasteurization temperatures range from 130-170F. The lower end of that range is not always sufficient in preventing survival of heat resistant molds. In contrast, when temperatures are too high or sustained for too long it destroys more of the beneficial bacteria which would normally survive. Manure and compost substrates are most vulnerable to this. A common result from excessive pasteurization temperatures is incomplete colonization of the bulk substrate (early pinning) followed by visible Trichoderma infection. Cross contamination after pasteurizationCauses: Mold during or after spawn run When spawning it's important to limit exposure of the spawn grains and pasteurized substrate to contaminants. Hands, clothes, and hair are major vector for recontamination of pasteurized substrate. Cooling substrate should be protected from dust as much as possible. Casing material is also vulnerable and contaminated casing material may lead to diseases like cobweb or verticillium. If using unpasteurized casing material it should be used straight from freshly opened or sealed bags. Insufficient gas exchange during spawn runCauses: Bacteria or stalling during spawn run Mold during spawn run Fermentation odor during spawn run A lack a gas exchange while bulk substrates are colonizing creates anaerobic conditions which can stall the recovery and colonization of the mushroom mycelium and promote the growth of anaerobic bacteria. A fermentation odor may develop. In serious cases the mushroom mycelium does not recover allowing mold to colonize the grains. Insufficient cooling of bulk substrateCauses: Bacteria or stalling during spawn run Mold during spawn run Mushroom mycelium is easily killed by high temperatures. All of the bulk substrate must be cooled to room temperature before spawning. - Unfinished *title edited 3/28/2020
Edited by fahtster (01/19/24 09:41 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Recognizing and dealing with contamination [Re: Kizzle] 7
#23130900 - 04/18/16 02:16 PM (7 years, 9 months ago) |
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Fantastic work Kizzle, stickied 
I would like to chime in that verticillium fungicola has been reclassified as Lencacillium fungicola to distinguish it from the verticillium species that attack plants.
Very nice work man.
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Grey
ā ⯠ā



Registered: 11/06/14
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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 3
#23131962 - 04/18/16 06:50 PM (7 years, 9 months ago) |
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Excellent write up Kizzle.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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myceliumEX
Philomathmycology



Registered: 12/10/15
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Re: Recognizing and dealing with contamination [Re: Grey] 4
#23132191 - 04/18/16 07:37 PM (7 years, 9 months ago) |
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Very easy to follow, and great info on how to identify contams.
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B.C. by Johnny Hart "All mushrooms are edible, but some only once." -- Croatian Proverb
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jimmyjams
Stranger

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Re: Recognizing and dealing with contamination [Re: myceliumEX] 2
#23133079 - 04/19/16 12:54 AM (7 years, 9 months ago) |
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Great post! I've bookmarked it.
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SpicyWizard



Registered: 12/14/15
Posts: 511
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Re: Recognizing and dealing with contamination [Re: jimmyjams] 2
#23143527 - 04/22/16 07:41 AM (7 years, 9 months ago) |
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great post! this is amazing, and ill deff be saving this as a bookmark!
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GreenPrayingMantis
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Re: Recognizing and dealing with contamination [Re: SpicyWizard] 2
#23201691 - 05/08/16 09:38 AM (7 years, 9 months ago) |
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thanks for the very good guide
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sauroman1
Emrys

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How to deal with bacteria endospores? I have suspicion that spore prints might be contaminated after repeatedly getting ruined PF cakes.
-------------------- "You come from realms of unimaginable power and light, and you will return to those realms.ā ā Terence McKenna
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Recognizing and dealing with contamination [Re: sauroman1] 2
#23225653 - 05/14/16 11:23 AM (7 years, 8 months ago) |
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Usually endospores are a problem in cereal grain and we deal with them via pressure cooking. If your inoculate is the vector then you either need to get new prints or start working with agar to clean it up. Actually agar is the better road IMO. At that point tho most people get pressure cookers and start with grains.
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sauroman1
Emrys

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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 2
#23230050 - 05/15/16 01:33 PM (7 years, 8 months ago) |
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Yes, agar dish has advantages, but used it only for cyanescens. Endospores can be problem in any nutritious enviroment. Question is what it's vulnerable sides compared to cube spores? Keeping in water for long time can eliminate spores? Don't have currently access to pressure cooker. But I've read that for Pf cakes it's not prerequisite.
-------------------- "You come from realms of unimaginable power and light, and you will return to those realms.ā ā Terence McKenna
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Kizzle
Misanthrope


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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 2
#23274034 - 05/26/16 01:56 PM (7 years, 8 months ago) |
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How is the bacteria manifesting? I mean is it showing up right away? Is it localized to the inoculation points or what?
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PsilocyBen17
Pin Pornographer


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Re: Recognizing and dealing with contamination [Re: Kizzle] 2
#23281741 - 05/28/16 03:47 PM (7 years, 8 months ago) |
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Sgt. Overkill
Agar Warrior


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Re: Recognizing and dealing with contamination [Re: PsilocyBen17] 2
#23355549 - 06/17/16 05:52 PM (7 years, 7 months ago) |
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Great guide! Very informative
-------------------- Sgt. Overkill of the 23rd Agar Division, Reporting for Duty!
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funkymonk22
In Service to the Ineffable..


Registered: 01/25/16
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Loc: The Big O
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Re: Recognizing and dealing with contamination [Re: Sgt. Overkill] 2
#23357709 - 06/18/16 11:14 AM (7 years, 7 months ago) |
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Yes, great post here...kinda answered the wuestion in my post, but hoping for a more specific answer to my problem...great job
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  "The clouds didn't look like cotton, they didn't even look like clouds.."-Townes Van Zandt
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fundyfresh
Just bubbles



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Re: Recognizing and dealing with contamination [Re: funkymonk22] 2
#23374176 - 06/23/16 03:34 PM (7 years, 7 months ago) |
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Any thoughts on this. After first flushhttp:/
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
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Re: Recognizing and dealing with contamination [Re: fundyfresh] 1
#23374252 - 06/23/16 03:56 PM (7 years, 7 months ago) |
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Trich. At least ya got a flush.
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Skillzd
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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 2
#23391770 - 06/28/16 05:46 PM (7 years, 7 months ago) |
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Great post. This is awesome. Hey I have a question. Sorry for the long text. Wanted to explain quickly. I was looking for a good place to check and I guess this is as good as any. Ok so here's what happened. Two of my cakes were too moist and started to ferment just a little. I make wine and know the smell well and caught it early. Mushrooms grow naturally around here and used to outback the neighbor said but someone dug a bunch up and never came back. So I knew a perfect spot and dumped the cakes out in some shade but where light is perfect. And hunidty and moisture is great. I sprayed them down a bit so they would be rinsed and maybe grow hopefully. I found a chunk of Mycellium growing about 2weeks later and it was under a leaf on a chunk of the cake I missed when rinsing. I grabbed it up carefully and put it in some pasturized horse poo/straw/coco coir/Verm. And put the chunk in the middle and layer of coco/Verm On top of it with some dry Verm too. I set it in an Aqaurium I had and slid it under my Motorhome so it wouldn't get rained on or in the sun. Today here's what it looks like. Is this anything I should keep still? It really looked like the proper nice white Mycelium but now since its come thru the casing layer it's looking a tad bit different and just wanted a few opinions on wether j should keep or toss
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Dubtubs420
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Re: Recognizing and dealing with contamination [Re: Skillzd] 2
#23478319 - 07/26/16 10:39 AM (7 years, 6 months ago) |
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Is this contamination? Pitching it regardless, other jar has no progress, can post more photos. Day 13 now for this jar.
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nakano
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Re: Recognizing and dealing with contamination [Re: Dubtubs420] 2
#23568780 - 08/23/16 10:47 AM (7 years, 5 months ago) |
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Basically anything not white, right?
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Shaker77
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Re: Recognizing and dealing with contamination [Re: nakano] 2
#23575511 - 08/25/16 09:49 AM (7 years, 5 months ago) |
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Good Morning, I was hoping that someone would be able to help me. I'm in my first flush and pining has started, however I have, what looks like some contamination occurring, on half of the surface,it looks like green mold, I tried to scoop it all out, but it just keeps coming back, should I salt that whole area, and hope for the best, or do I just let it go for now. Any help from someone with more knowledge then me would be greatly appreciated. Looking forward to hearing from someone. Thank-you
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Grey
ā ⯠ā



Registered: 11/06/14
Posts: 6,223
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Re: Recognizing and dealing with contamination [Re: Shaker77] 1
#23577721 - 08/25/16 09:17 PM (7 years, 5 months ago) |
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If it's green, it'll likely keep coming back. I would toss it or bury outside.
If you're dead set on getting fruits, and it's still growing them, maybe don't keep it around your workspace/grows.
--------------------
AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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SilentMajority420
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Re: Recognizing and dealing with contamination [Re: Grey] 1
#23609729 - 09/03/16 11:02 PM (7 years, 5 months ago) |
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Hello individual people, and the One Cosmic Universe; same thing.
I have encountered the ghostly green gunk, and it was not pleasant. That smothering smug smell, which permeates it putrid spores over all that which is holy! The madness must cease, if I wish to find peace for me and my mushroom. So this brave knight woketh early when the cock crowed, and boomed "the cleansing will commence in this humble abode!"
So yea, woke up early, went to lowes, acquired equipment, and cleaned the ever living fuck out of my room. 10:1 H20/bleach sprayed the walls, containers, ceiling, everything. Moved 90% of stuff to basement, and kept the essentials in a single container. Cleaned the carpet, sprayed the carpet, ejaculated, but later cleaned up, on the carpet. Fucking hospitals ain't got shit on how sterile my room and closet is bruh. It better be since I spent 12 hours on that ass. No joke. Which makes me realize, now, several failed attempts later, why we need to listen to the experience 'old timers'. Growing is serious business. We will definitely get to play, but, we must first work diligently all the way through, before we blast off. We should treat it like we're building an actual rocket, to help us grow our metaphorical. Every nut and bolt is accounted for before ignition, and inspected multiple times. There are millions of dollars, and crew's lives at stake, so we must take every precaution. That is the way we should approach it; period.
I am going to vanquish thy enemy! No trich bitch is going to ruin my fun!
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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You're kinda going about it wrong. If you're having issues with trich, cleaning your room isn't the answer. Working on clean spawn and clean agar inoculant is. You only need to be sterile when opening media. That means in your still air box or in front of a hood. Clean rooms have nothing to do with still air box work
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SilentMajority420
Stranger

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Re: Recognizing and dealing with contamination [Re: Mad Season] 1
#23611395 - 09/04/16 01:27 PM (7 years, 5 months ago) |
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Interesting.
I've been browsing this forum and cultivating for the past few months, and only now am I finding out about this magic 'agar'. It really helps to post and receive responses from experienced users, instead of just watching from the sidelines. Thanks for your help.
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Bhbuda
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#23745426 - 10/17/16 12:16 PM (7 years, 3 months ago) |
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I'm new here and freaking out about what I have here. Sorry to restart an old post, but this seems the best place. So in the pictures you can see the colour of the caps are weird, and there are green streaks in the stems. Basically what could cause this, and is it safe to eat? Sorry again for being a forum noob.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Recognizing and dealing with contamination [Re: Bhbuda] 1
#23745432 - 10/17/16 12:18 PM (7 years, 3 months ago) |
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Mostly bruising. Looks like there's some insane vert in there. Hella fuzzy feet from no fresh air too, which is what vert thrives in.. is this a kit done in a bag? Those things have the worst conditions for mushrooms. Make a proper fruiting chamber like sgfc.
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Bhbuda
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Re: Recognizing and dealing with contamination [Re: Mad Season] 1
#23745461 - 10/17/16 12:30 PM (7 years, 3 months ago) |
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Thanks for the fast reply. Yea, they a the tubs in the bags which you only have to flower (I'm lazy). Used them a few times before, but never looked like this, normally I get better fresh air so I don't get the white fuzz so much. This one just went very wrong. If these were yours, would you consume them?
Also, I'm 99% sure they were ment to have brown/golden tops. Is the white heads due to the same infection?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
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Re: Recognizing and dealing with contamination [Re: Bhbuda] 1
#23745515 - 10/17/16 12:50 PM (7 years, 3 months ago) |
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Looks like it could just be leucism since they're still sporulating purple spores. There's a few strains which seem prone to this. Like mckenaii, and the obvious AA+
Vert is 100% edible, and almost never gets properly identified, yet nobody goes to hospital from bad medical shit. I'm just saying one of them are exhibiting the dots at the base of the stem, which is a verticillium indicator. I wouldn't print these guys either. Just take better care of them, and grow properly next time them suckers love air. More than humidity tbh.
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Bhbuda
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Re: Recognizing and dealing with contamination [Re: Mad Season] 1
#23745540 - 10/17/16 12:58 PM (7 years, 3 months ago) |
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So basically like an albino mushroom? Cool!
These ones are produced by "supa grow" and the type was "Brazil". Thank you so much, helped me with my stress levels. Sorry once again if I didn't use this section properly.
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Dlurch314
Stranger

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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#23841932 - 11/17/16 02:15 PM (7 years, 2 months ago) |
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Any idea what this is?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
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Re: Recognizing and dealing with contamination [Re: Dlurch314] 1
#23841986 - 11/17/16 02:29 PM (7 years, 2 months ago) |
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Trich
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Dlurch314
Stranger

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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 1
#23842059 - 11/17/16 02:51 PM (7 years, 2 months ago) |
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It's not green though. If it is trich is there anything I can do?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
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Loc: Canada
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Re: Recognizing and dealing with contamination [Re: Dlurch314] 1
#23842287 - 11/17/16 03:44 PM (7 years, 2 months ago) |
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Depending on a few factors trich before pins means the spawn was fucked and the whole tub will green up in no time. You can salt trich if the flush is almost done to hold it off for a day or two but this early in the game means it's probably all trash.
You can hang on to it for a day if you want to be sure, it will probably be green in a day and you will know for sure.
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Dlurch314
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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 1
#23842585 - 11/17/16 05:02 PM (7 years, 2 months ago) |
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Yeah this one just started pinning a couple of days ago
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Chickie
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Re: Recognizing and dealing with contamination [Re: Dlurch314] 1
#23850888 - 11/20/16 09:27 AM (7 years, 2 months ago) |
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Hello. This is my first grow. Started from a kit with 18 jars and GT spore syringes. Inoc on 11/12. Colonization observed on some jars 3 days in. Today, 8 days after inoc, all jars showing colonization with 2 jars with very minimal signs. All jars have what appear to be healthy mycelium. Notice one jar today with yellowing spots. Can anyone please suggest whether this is contaminate or water damage? My understanding is water damage appears yellow. Also, the area that is yellow appears wet...wetter then rest of jar, if you will. Please see pic. I have isolated the suspect jar from incubator for now. Thanks for your help.
Edited by Chickie (11/20/16 07:41 PM)
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Mistylee
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Re: Recognizing and dealing with contamination [Re: Kizzle] 2
#23852515 - 11/20/16 07:23 PM (7 years, 2 months ago) |
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Moved over a fruiting jar (dunked it) a few days ago and it seems kinda fuzzy today. I looked through all of the contaminant images and couldn't find any that look like this. Just wanted to post this and get some thoughts. The other jars were just moved in today.


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psychedethiogenic
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Re: Recognizing and dealing with contamination [Re: Mistylee] 1
#23892152 - 12/03/16 09:29 PM (7 years, 2 months ago) |
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Can anyone please tell me if it is possible for me to save this jar, or should I just toss iht?


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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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That is completely fucked.
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SpicyWizard



Registered: 12/14/15
Posts: 511
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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 1
#23892769 - 12/04/16 02:53 AM (7 years, 2 months ago) |
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Quote:
Pastywhyte said: That is completely fucked.

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psychedethiogenic
Stranger
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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 1
#23893393 - 12/04/16 10:39 AM (7 years, 2 months ago) |
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Do you happen to know what went wrong? I took extra sterilization caution and kept the jar at around 73 to 75 degrees although I think at one point the temp rose to at least 80 degrees if not more. I also noticed quite a bit of moisture.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Hard to say. If it's in every jar it's probably a bad syringe or bad inoculation procedure.
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krudam2
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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 1
#23939886 - 12/19/16 08:51 AM (7 years, 1 month ago) |
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thanks for the post.
could we get a picture of metabolites?
I don't know the difference between normal metabolites and infection, and I've had a commercial oyster bag (bought at store) have a large orange bit but customer service told me it was fine and it seemed to grow fine
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The Mycologist
Explorer

Registered: 05/06/16
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Well whats the deal with that lid?
-------------------- "That you are hereāthat life exists, and identity; That the powerful play goes on, and you will contribute a verse.ā ā Walt Whitman, Leaves of Grass

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Kizzle
Misanthrope


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Re: Recognizing and dealing with contamination [Re: krudam2] 1
#23984419 - 01/05/17 04:31 PM (7 years, 1 month ago) |
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Quote:
krudam2 said: thanks for the post.
could we get a picture of metabolites?
I don't know the difference between normal metabolites and infection, and I've had a commercial oyster bag (bought at store) have a large orange bit but customer service told me it was fine and it seemed to grow fine
I added some. Metabolites normally appear in very amounts as in the pics but if the substrate has fully colonized for quite a while or it's been sitting in particularly warm conditions you might see more. If you're seeing large puddles or uncolonized areas then it could still be metabolites your seeing but probably the result of a contaminant.
--------------------
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Scottishwarrior
Grower



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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#23987543 - 01/06/17 07:26 PM (7 years, 1 month ago) |
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Excellent article. I always send people the link to this thread when they ask questions about contamn.
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Jennifer1
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#23998459 - 01/10/17 02:53 PM (7 years, 1 month ago) |
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Smells like coconut! Thank you
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Mushenstein
Brain Damage



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Loc: At The Gates
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Re: Recognizing and dealing with contamination [Re: Jennifer1] 1
#24063715 - 02/03/17 09:22 PM (7 years, 13 days ago) |
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Wow, That was an impressive read. Damn fine job.
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Aquatic Wombat
Homie

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Re: Recognizing and dealing with contamination [Re: Mushenstein] 1
#24198792 - 03/28/17 02:48 AM (6 years, 10 months ago) |
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I'm a total noob at this. This is my first attempt. I had 2 spore syringes and decided to inoculate 3 cakes and jar of liquid per syringe. I sterilized a small room, enclosed it with plastic sheeting, spritzed everything with a bleach solution, used a sterilized glove box, wore a tyvex suit, gloves, a respirator, flame sterilized the needle with an alcohol burner, sterilized the cakes in a pressure cooker @15 p.s.i. for 20 min. and the liquid for 15 to avoid caramalezation and after inoculation everything has been between 70-80 F. Mycelium growth in all six cakes appears fine after 4 days. One of the liquid cultures appears to have no activity while the other has this puffy ball with black spores from the syringe on it. Is this F.U.B.A.R.? Should I inoculate one new cake and see what happens, or should I just pitch it? Thanks.
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Aliceinwonderland3

Registered: 03/29/17
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Last seen: 6 years, 6 months
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#24203225 - 03/29/17 05:07 PM (6 years, 10 months ago) |
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I was also hoping to identify this "stuff" in my one jar... Not to hopeful about it :-(
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
Aquatic Wombat said:

Defintely pitch, try to germinate and clean on agar before trying liquids.
Quote:
Aliceinwonderland3 said: I was also hoping to identify this "stuff" in my one jar... Not to hopeful about it :-(

Idk it down to the species or even the genus, but it's definitely not clean. Looks like aspergillus maybe.
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Zachorion


Registered: 03/06/17
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#24203642 - 03/29/17 07:48 PM (6 years, 10 months ago) |
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My first Pasty Plate agar try. I'm guessing this is Penicillium? It's the only one so far so I'm hoping the others are viable.
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dart21
Stranger
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Re: Recognizing and dealing with contamination [Re: Zachorion] 1
#24225537 - 04/07/17 12:55 PM (6 years, 10 months ago) |
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Probs Zach, but cannot be certain from that photo.
Edited by dart21 (04/07/17 12:57 PM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,930
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Re: Recognizing and dealing with contamination [Re: dart21] 1
#24233674 - 04/10/17 11:05 PM (6 years, 10 months ago) |
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1. Should I be concerned or worried about the cloudier, more solid area in the middle of the plate? I always notice my initial transfer wedge, being higher up than the level plate surface, bubbles up cloudy-like until it flattens out on the receiving plate's horizon. Is that what I'm seeing here?
2. Should the big divide between 11 and 12 concern me?
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Dlurch314
Stranger


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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#24234306 - 04/11/17 09:06 AM (6 years, 10 months ago) |
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Hey guys in not sure if this is bruising or some kind of contam any ideas?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Recognizing and dealing with contamination [Re: Dlurch314] 1
#24237185 - 04/12/17 10:42 AM (6 years, 10 months ago) |
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Bruising because its hella contaminated with bacteria
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Dlurch314
Stranger


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Re: Recognizing and dealing with contamination [Re: Mad Season] 1
#24237454 - 04/12/17 12:57 PM (6 years, 10 months ago) |
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So what would you do in my case? Would you pitch it or give it a shot?
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,930
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Re: Recognizing and dealing with contamination [Re: Dlurch314] 1
#24237630 - 04/12/17 02:05 PM (6 years, 10 months ago) |
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lol, absolutely get rid of it. Give what a shot? Releasing bacteria into your living area? That's not a shot; it's a guarantee.
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The Mycologist
Explorer

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Re: Recognizing and dealing with contamination [Re: stareatclouds] 1
#24237645 - 04/12/17 02:11 PM (6 years, 10 months ago) |
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Yea i dont like the look of that, transfer away from the cloudiness
-------------------- "That you are hereāthat life exists, and identity; That the powerful play goes on, and you will contribute a verse.ā ā Walt Whitman, Leaves of Grass

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Dlurch314
Stranger


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Re: Recognizing and dealing with contamination [Re: stareatclouds] 1
#24241042 - 04/13/17 05:17 PM (6 years, 9 months ago) |
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Already in the dumpster thanks man
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outofservice
I never get to touch no monkey

Registered: 04/27/17
Posts: 160
Loc: Sun & Sand
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#24470851 - 07/09/17 06:10 PM (6 years, 7 months ago) |
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Good info, thanks.
After reading this thread I thought these were contaminated, need a second opinion.
They are Pan. Cyan Cambo Sandose. This is my first grow. Noc on 5-17, I had two jars start colonizing almost a month after inoc. I've had the rest of the jars sitting in a dark closet waiting for the last two to get to 100%. The closet averages 77-80F, 40-46% humidity. I've been told the yellow circle is a sclerotia. I hope it is, that would be a cool surprise.
I'm growing these because they're f*cking awesome! A friends friend grew them some time ago and I was hooked. So now I'm trying to grow them. I had my friend look at them and he told me he thought he remembered bluing when his friend grew them.
What are your opinions? Good or toss? Anything I can do to save them? Let them sit under a UVC light if needed?
If they're f*cked, is it because I let them sit too long?
I read this in another thread, "Dip them into a sol'n of %10 H2O2, drain them, and return them to their jars." Is this an option?

Edited by outofservice (07/10/17 07:56 PM)
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outofservice
I never get to touch no monkey

Registered: 04/27/17
Posts: 160
Loc: Sun & Sand
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#24470861 - 07/09/17 06:15 PM (6 years, 7 months ago) |
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Is this Bacillus? If no, what is it? 5 grain, Mexis Jalisco. Trying for stones.
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outofservice
I never get to touch no monkey

Registered: 04/27/17
Posts: 160
Loc: Sun & Sand
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#24473617 - 07/10/17 07:34 PM (6 years, 7 months ago) |
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Hey All,
I plan to birth, dunk and roll tmw.
If I could some feedback on my post above with the pics (Pan Cyan Cambo), it would be greatly appreciated. I don't want to risk contaminating the other jars I have (Mex jalisco and Tampanensis).
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stewboy350
Stranger


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Re: Recognizing and dealing with contamination [Re: outofservice] 1
#24483771 - 07/14/17 08:51 PM (6 years, 7 months ago) |
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http:// cobweb ?
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outofservice
I never get to touch no monkey

Registered: 04/27/17
Posts: 160
Loc: Sun & Sand
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Re: Recognizing and dealing with contamination [Re: Kizzle] 2
#24514029 - 07/28/17 01:25 PM (6 years, 6 months ago) |
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This
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Steevo
Just a cog in the death wheel



Registered: 03/02/17
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Re: Recognizing and dealing with contamination [Re: outofservice] 1
#24514083 - 07/28/17 01:50 PM (6 years, 6 months ago) |
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Those grains look a little milky in that second pic. I haven't seen Jalisco colonizing before so it could be fine Why are you planning on birthing, dunking and rolling that? You can just let it go in the bag for stones
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outofservice
I never get to touch no monkey

Registered: 04/27/17
Posts: 160
Loc: Sun & Sand
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Re: Recognizing and dealing with contamination [Re: Steevo] 1
#24514949 - 07/28/17 08:45 PM (6 years, 6 months ago) |
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Quote:
Steevo said: Those grains look a little milky in that second pic. I haven't seen Jalisco colonizing before so it could be fine Why are you planning on birthing, dunking and rolling that? You can just let it go in the bag for stones 
The bag is for stones. I had a guy who is familiar with the Mexicans say it does colonize in a funky way. Regardless, I'll let it be and ride it out, maybe I'll get some stones. The jars are what I dunked and cased.
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margaretlcarlson
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Re: Stinky cheese smell. [Re: Kizzle] 1
#24568191 - 08/20/17 07:54 PM (6 years, 5 months ago) |
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First of all let me apologize, I am brand new to this site and to cultivating mushrooms. I am finding the site a challenge to navigate. I inoculated two agar plates one week ago and while as far as I can tell the growth on the plates looks normal, they smell strongly of stinky cheese, the mushy, aged kind. I don't know what, if anything, they are supposed to smell like.
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Crispykoot
Jello Wrangler



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Posts: 5,922
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Last seen: 1 day, 18 hours
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Quote:
margaretlcarlson said: First of all let me apologize, I am brand new to this site and to cultivating mushrooms. I am finding the site a challenge to navigate. I inoculated two agar plates one week ago and while as far as I can tell the growth on the plates looks normal, they smell strongly of stinky cheese, the mushy, aged kind. I don't know what, if anything, they are supposed to smell like.
Welcome to Shroomery!
Always post pictures if you can...It helps people help you. Don't open your plates up to smell them. That will contaminate them.
--------------------

Shadowboxing the apocalypse and wandering the land
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KrombopulosM
Mycoshaman



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Re: Recognizing and dealing with contamination [Re: Crispykoot] 1
#24652494 - 09/22/17 08:09 PM (6 years, 4 months ago) |
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Please help me identify if this aerial rhizo is caused by bacteria of some sort

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Pastywhyte
Say hello to my little friend



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Re: Recognizing and dealing with contamination [Re: KrombopulosM] 1
#24652496 - 09/22/17 08:10 PM (6 years, 4 months ago) |
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Maybe a little stressed but nothing to fret over. Will probably be fine.
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KrombopulosM
Mycoshaman



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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 1
#24654159 - 09/23/17 04:59 PM (6 years, 4 months ago) |
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thanks, i just made some AA so im gonna transfer to those from my 3rd gen to hopefully get contam free myc for gen 4
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Koka
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] 1
#24670345 - 09/29/17 02:33 PM (6 years, 4 months ago) |
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 [url=https://files.shroomery.org/files/17-39/671678266-20170928_151102.jpg][image]http://www.shroomery.org/forums/thumbs/17-39/671678266-thumb_20170928_151102.jpg[/image[url=http://files.shroomery.org/files/17-39/671677843-20170928_151050.jpg] Hi guys can anyone tell me are these mushrooms bad or good ? Its my first time growing have no clue. thanks for help
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paegan
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Re: [STICKY] Recognizing and dealing with contamination [Re: Koka] 1
#24685602 - 10/05/17 10:41 AM (6 years, 4 months ago) |
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Contam or no contam?
-------------------- Virtual Reality, Analog
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ChaostoOrder
Lakota Peji Wicasa,



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Re: Recognizing and dealing with contamination [Re: paegan] 1
#24744651 - 10/29/17 02:03 AM (6 years, 3 months ago) |
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Beautiful Sticky thank you very much!! I believe one needs to study the contaminates just as much, if not more, than the actual species being attempted or identified. This is very informational thank you!! good job!!!
-------------------- Too weird to live, to rare to die....-Hunter S. Thompson

: [url=https://files.shroomery.org/files/10-21/47399604
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Radagastthebrown



Registered: 10/22/17
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Loc: United states
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] 1
#24816299 - 11/30/17 08:31 AM (6 years, 2 months ago) |
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Now this here is very helpful and I think everyone needs to read this a few times especially beginners or anyone who wants to truly understand mycology. Bookmarked and read a few times, will be passing on the info. Thank you for this.
Edited by Radagastthebrown (11/30/17 08:33 AM)
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fukuzi
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#24829305 - 12/06/17 06:11 AM (6 years, 2 months ago) |
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Hello yellow, some pro outlook is required on these PFtek thingys. They've been around 4 weeks @ 23-28celcius, how do they look like and do they have a bright future? 1 of them shows not much signs of life, but what about these 2 others?
So they 2 are mostly white, but there is some yellowish colors and little bit black. This is a first try, so some pro comments would be really important.
Side A
 Side B
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mushroom_mode
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Re: Recognizing and dealing with contamination [Re: fukuzi] 1
#24862473 - 12/22/17 04:50 PM (6 years, 1 month ago) |
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can a contaminated (unmodified) tub infect a nearby healthy tub if both lids are ajar?
is it worth trying to fruit a tub with a light alcohol odour when the lid is opened?
using coir + oats
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beltain
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] 1
#25095261 - 03/27/18 04:55 PM (5 years, 10 months ago) |
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so many ways for it to go wrong! I'm gonna try to grow soon and hope to encounter none of these guys. Thanks for the excellent write-up!
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TheGreatGonzo
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] 1
#25140648 - 04/15/18 09:54 AM (5 years, 9 months ago) |
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Thanks for the info Kizzle, so much knowledge in one place!
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JiM aYYYY
GreenGoblin


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Re: [STICKY] Recognizing and dealing with contamination [Re: TheGreatGonzo] 1
#25147891 - 04/18/18 11:36 AM (5 years, 9 months ago) |
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 This is my first tray. I over misted and made some pools on accident overnight. Lesson learned.
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Groggybandito
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] 1
#25170085 - 04/27/18 07:35 PM (5 years, 9 months ago) |
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Is this contamination, it is in a shotgun furting chamber, 99% R/H, fan 5-6 times a day, been in the chamber for 3 days, brown rice flower verm,
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raoulduke948
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Re: Recognizing and dealing with contamination [Re: Groggybandito] 1
#25195105 - 05/10/18 09:13 AM (5 years, 9 months ago) |
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what do you think about those , they are contamined or just bruising ?
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JiM aYYYY
GreenGoblin


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Re: Recognizing and dealing with contamination [Re: raoulduke948] 1
#25195129 - 05/10/18 09:32 AM (5 years, 9 months ago) |
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Quote:
raoulduke948 said: what do you think about those , they are contamined or just bruising ? 
Contamination for sure. Sorry for the loss man
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raoulduke948
Stranger


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Re: Recognizing and dealing with contamination [Re: JiM aYYYY] 1
#25195541 - 05/10/18 12:57 PM (5 years, 9 months ago) |
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I will restart one of them , and one i cleaned , i hope it's gonna be ok , i will come with news soon , cheers ! And thx for support !
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SamAtticus

Registered: 02/27/18
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Fantastic [Re: Kizzle] 1
#25198690 - 05/11/18 11:54 PM (5 years, 9 months ago) |
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Amazingly helpful (and unnerving) index, especially useful are the pics of various contaminants. This must have taken months to put together. Fantastic work!
I must admit, though, I'm a little confused by this paragraph:
"Overlay - A common sign of Trichoderma is mycelium that colonizes over a casing layer or the vermiculite fruiting PF cakes have been rolled in. While mycelium can also create overlay, if your fruiting conditions have been adequate to prevent it, this can be one of the first signs you notice."
I'd love some clarification if and when you have the time or inclination.
Again, beautiful work! Thank you!
--------------------
  "I learn by going where I have to go." Theodore Roethke Bod's AF Search Engine Tek
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Gonzo the Eternal
In Sterquiliniis Invenitur

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Great write up! And yes I agree with the guy up top, unnerving as well.
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JiM aYYYY
GreenGoblin


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 What type of contamination would you call this?
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nix21
Stranger



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Re: Recognizing and dealing with contamination [Re: JiM aYYYY] 1
#25235804 - 05/29/18 10:15 AM (5 years, 8 months ago) |
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I found some weird spot in my BRF cake, i don't know can you tell me whats going on with this pictures.. today is 19 day after inoculation (koh samui, ms syringe) and everything looks great, but this spot!.. i can tell for sure nothing is growing there (for now) it looks like a bit wet and maybe missing some brown rice flour, but I'm 80% sure that wasn't there few days ago.. also those in picture that looks like black dots in that area isn't black dots but holes in verm/brf mixture.. also i steam sterilized 9 jars for 2 hours... all doing great... and this is 277ml jar if that's important..



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Kizzle
Misanthrope


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Quote:
SamAtticus said: Amazingly helpful (and unnerving) index, especially useful are the pics of various contaminants. This must have taken months to put together. Fantastic work!
I must admit, though, I'm a little confused by this paragraph:
"Overlay - A common sign of Trichoderma is mycelium that colonizes over a casing layer or the vermiculite fruiting PF cakes have been rolled in. While mycelium can also create overlay, if your fruiting conditions have been adequate to prevent it, this can be one of the first signs you notice."
I'd love some clarification if and when you have the time or inclination.
Again, beautiful work! Thank you!
Thanks for pointing that out. In part it's confusing because I meant to upload some better pictures later but I also rewrote it so it makes more sense. Basically what I meant is when you notice mycelium growing over parts of the substrate you normally don't see it growing particularly in comparison with identically made substrates. When you have an actual casing layer you normally aim for it to not be colonized, and to mostly have rhizomorphs growing through it but then suddenly there's this patch growing over it and...
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nube424

Registered: 12/03/17
Posts: 6,063
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Quote:
JiM aYYYY said:
 What type of contamination would you call this?
Um I think it's contaminated with psilocybe cubensis?
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tombonytom
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Re: Recognizing and dealing with contamination [Re: nube424] 1
#25312278 - 07/06/18 07:18 PM (5 years, 7 months ago) |
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just made a liquid culture with honey, took my old liquid culture which has proven success and a lot of it. but I'm quite new to lc, I know it's bad, but why not take the challenge.
my problems are, mycelium doesn't really float, more like dingle to the bottom.. I have kind of frisbee made of mycelium, which floats and looks like something I could pour out of an old bottle of milk..
it came from a clean culture, should I dump? or just add some absinth, put on my pirate wood leg, chuck it and yell at the neighbors?
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BloneDude
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Re: Recognizing and dealing with contamination [Re: tombonytom] 1
#25322887 - 07/12/18 07:01 PM (5 years, 7 months ago) |
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Hey yall,
I did not want to create another thread since it appears many people have posted on this thread for help in identifying potential contamination. I am currently harvesting some fruit, but I noticed a few spots on a variety of cakes that are giving me some concern. I took some photos, and if I did this right they should load. If you could provide any assistance in letting me know if it could be something, or if I am just being over cautious it would be greatly appreciated!
Shot 1:

Shot 2:

Shot 3:

Shot 4:

Thanks for any help, and much love to you all!
-------------------- Selin
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Kizzle
Misanthrope


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Re: Recognizing and dealing with contamination [Re: BloneDude] 2
#25323387 - 07/13/18 01:55 AM (5 years, 7 months ago) |
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It's just mycelium caused by the higher Co2 levels near the substrate and blue bruising from the substrate drying out.
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#25323608 - 07/13/18 07:25 AM (5 years, 7 months ago) |
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What I wanna know is, is everything we call trich actually trich? Or is most of it actually penicillin and we just can't tell the difference?
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FunGuyZ
Psilly Rabbit


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Re: Recognizing and dealing with contamination [Re: nube424] 1
#25339310 - 07/21/18 03:39 PM (5 years, 6 months ago) |
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Is this Trich- bad feeling
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AnkhSaisRaKhamun
hermit
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Re: Recognizing and dealing with contamination [Re: Grey] 1
#25374770 - 08/08/18 11:17 AM (5 years, 6 months ago) |
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what happens when all growth stops and is dry?
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Recognizing and dealing with contamination [Re: AnkhSaisRaKhamun] 1
#25374809 - 08/08/18 11:36 AM (5 years, 6 months ago) |
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Then u throw it out. And start over.
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IncreduLuck
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Re: Recognizing and dealing with contamination [Re: nube424] 1
#25380873 - 08/11/18 03:35 AM (5 years, 6 months ago) |
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Hey guys, newb cultivator here.. This started and has been growing for like a week on the side of my container.. Do you think it's the aerial rhyzomorphs or something more serious? It's in strands and seem to grow upwards. Picture:
Edited by IncreduLuck (08/11/18 12:48 PM)
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Recognizing and dealing with contamination [Re: IncreduLuck] 1
#25381370 - 08/11/18 10:17 AM (5 years, 6 months ago) |
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Upload the pic to the site. The little mountain icon over there when ur posting ------->>
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IncreduLuck
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Re: Recognizing and dealing with contamination [Re: nube424] 1
#25381577 - 08/11/18 12:49 PM (5 years, 6 months ago) |
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Alright, I edited my previous post to include it. Thanks for the instructions!
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Kizzle
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Re: Recognizing and dealing with contamination [Re: IncreduLuck] 1
#25382565 - 08/11/18 09:54 PM (5 years, 6 months ago) |
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It's just mycelium, and it looks like there are some knots forming nearby
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IncreduLuck
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#25384831 - 08/13/18 02:21 AM (5 years, 6 months ago) |
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Thanks for the advice! It's just weird to see mycelium grow up from the medium.
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SamAtticus

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Re: Fantastic [Re: Kizzle] 1
#25421729 - 08/29/18 02:48 PM (5 years, 5 months ago) |
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Quote:
Kizzle said:
Quote:
SamAtticus said: Amazingly helpful (and unnerving) index, especially useful are the pics of various contaminants. This must have taken months to put together. Fantastic work!
I must admit, though, I'm a little confused by this paragraph:
"Overlay - A common sign of Trichoderma is mycelium that colonizes over a casing layer or the vermiculite fruiting PF cakes have been rolled in. While mycelium can also create overlay, if your fruiting conditions have been adequate to prevent it, this can be one of the first signs you notice."
I'd love some clarification if and when you have the time or inclination.
Again, beautiful work! Thank you!
Thanks for pointing that out. In part it's confusing because I meant to upload some better pictures later but I also rewrote it so it makes more sense. Basically what I meant is when you notice mycelium growing over parts of the substrate you normally don't see it growing particularly in comparison with identically made substrates. When you have an actual casing layer you normally aim for it to not be colonized, and to mostly have rhizomorphs growing through it but then suddenly there's this patch growing over it and...

Thank you so much for the clarification! Much appreciated.
--------------------
  "I learn by going where I have to go." Theodore Roethke Bod's AF Search Engine Tek
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Friedmind
Stranger than thou

Registered: 07/20/18
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Looking for verification. [Re: Kizzle] 1
#25436984 - 09/05/18 08:26 AM (5 years, 5 months ago) |
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First time for everything first grow, first flush, new member, and first post. I am using rye berries in 110 quart mono tubs, with six 2 1/2 inch air exchange holes, three high and three low all stuffed with poly. Everything has gone well until I put my myc in the mono tub. The first time I watered them I bruised one tub very badly and the other not so much. Thought I had contamination and freaked out. Now thanks to this site, I have learned to mist my tubās and I have pins popping all over. Oh yes these are golden teachers on day 7 since the first pins appeared. I have a couple with fuzzy feet that I think is from poor fae, but with my poly and I fan them with each misting(up to 5 times/day if Iām home) I want a more experienced opinion. Thanks in advance and Thank You greatly for all the info on this site.
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Looking for verification. [Re: Friedmind] 1
#25444361 - 09/08/18 04:41 AM (5 years, 5 months ago) |
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It's very dry in there. Awesome job for ur first grow though!!!! Next time try making the poly on the bottom a lot tighter to hold the humidity in. If u dial in the tub right u shouldn't have to mist much.
Fuzzy feet are hard to avoid too. And it can happen from misting too. Ur on the right track tho man, I can tell uve been reading for awhile
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Friedmind
Stranger than thou

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Re: Looking for verification. [Re: nube424] 1
#25450519 - 09/10/18 07:06 PM (5 years, 5 months ago) |
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Thank you. Glad to hear Iām doing something right lol. First flush gave me just over 5oz of dehydrated product between the two tubs. Somewhere I read to pluck em soon as the veil tears, so I ended up with a bunch of little ones. Towards the end of the flush I read that it doesnāt hurt to let them spread their caps, the last few were huge. Any input as to which way is best?
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Looking for verification. [Re: Friedmind] 1
#25451603 - 09/11/18 05:57 AM (5 years, 5 months ago) |
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They say for potency it's best to pick early. And f ur trying to avoid spores all over ur shrooms. Otherwise it doesn't hurt to let them open. If u want max weight without getting spores everywhere, pick the ones with open caps b4 they drop spores everywhere, and leave the rest till they start to open up.
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Friedmind
Stranger than thou

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Re: Looking for verification. [Re: nube424] 1
#25478413 - 09/21/18 04:20 PM (5 years, 4 months ago) |
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At the end of my second flush I had spores drop, now 3 days later there is no new growth except for this (see picture) in one corner. Is my tub done for? Or can I cut out this corner and see what happens?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Looking for verification. [Re: Friedmind] 1
#25478415 - 09/21/18 04:22 PM (5 years, 4 months ago) |
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Quote:
Friedmind said:
At the end of my second flush I had spores drop, now 3 days later there is no new growth except for this (see picture) in one corner. Is my tub done for? Or can I cut out this corner and see what happens?
No it's done. Put it outside, it's compost now.
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Wing
The Eye Tyrant



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Re: Looking for verification. [Re: Pastywhyte] 1
#25478872 - 09/21/18 06:50 PM (5 years, 4 months ago) |
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Quote:
Pastywhyte said:
Quote:
Friedmind said:
At the end of my second flush I had spores drop, now 3 days later there is no new growth except for this (see picture) in one corner. Is my tub done for? Or can I cut out this corner and see what happens?
No it's done. Put it outside, it's compost now.

Caught a case o' Trichoderma
-------------------- My Old Grow Logs
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orionstarseed
Stranger

Registered: 09/22/18
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] 1
#25487094 - 09/25/18 02:29 AM (5 years, 4 months ago) |
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I have just tried to inoculate some jars with two different spore syringes on the 20th and I have started to have Cobweb mold in them. Do you think my syringes are bad or what is going on? It seems the cobweb mold starts at about the 3-4 day mark and I don't believe it is the syringes because the mold started on the outside of the cakes and I inoculated them straight down into the substrate as I did not know I was supposed to inoculate towards the glass. What is going on and how can I prevent this? This is my first time doing this and so far it has been very dissapointing, I already have to chuck out 5 jars and I don't want to waste anymore solution or jars.
Edited by orionstarseed (09/25/18 02:31 AM)
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: [STICKY] Recognizing and dealing with contamination [Re: orionstarseed] 1
#25487400 - 09/25/18 07:32 AM (5 years, 4 months ago) |
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Sterilize ur jars longer. Cobweb mostly comes from not sterilizing well enough ime.
And yes, next time inoculate toward the glass so the water drips down the glass. It's easier to monitor growth.
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alorac
Stranger
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] 1
#25502196 - 09/30/18 11:06 PM (5 years, 4 months ago) |
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Newbie question:
When I cut my shrooms to dry, I noticed blue-green everywhere I cut. Is this contamination? Is it bruising? Are these safe to eat?
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Wing
The Eye Tyrant



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Re: [STICKY] Recognizing and dealing with contamination [Re: alorac] 1
#25502202 - 09/30/18 11:12 PM (5 years, 4 months ago) |
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Its bruising and safe to eat
-------------------- My Old Grow Logs
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Gris
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Re: [STICKY] Recognizing and dealing with contamination [Re: Wing] 1
#25529114 - 10/11/18 08:17 AM (5 years, 4 months ago) |
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Getting started with agar and this pink dot showed up on one of my plates.
Anybody that could help identify and can I just cut it out of the plate ?

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dmar


Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#25531024 - 10/11/18 09:57 PM (5 years, 4 months ago) |
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On the list of non contaminants, you should include primordia. I had a bunch of yellow/brownish blobs form in my jars, and I thought it was some sort of bacterial infection. Come to find out it was primordia that eventually turned into knots and pinned. Probably just a rookie mistake, but since the point of this forum is helping rookies, it might be worth adding. Just a thought!
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mayot02
Stranger

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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] 1
#25540338 - 10/15/18 07:56 PM (5 years, 4 months ago) |
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what do you think? is there mold present? this is after the 2nd surge of mushrooms. there is a white fuzz growing that is new
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: [STICKY] Recognizing and dealing with contamination [Re: mayot02] 1
#25541137 - 10/16/18 05:04 AM (5 years, 3 months ago) |
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That's thrich. It'll be green shortly. Good eye. Most new people don't notice mold till it changes colors.
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dmar


Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
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Re: [STICKY] Recognizing and dealing with contamination [Re: nube424] 1
#25549934 - 10/19/18 10:59 AM (5 years, 3 months ago) |
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I got a nice flush out of these cakes, but this one had a good bit of blue bruising throughout the whole process. I dunked and rolled in verm for a second flush last night and about 14 hours later, I noticed that this patch where it was very blue is now looking awfully dark and almost gray. I think it's just bruising getting worse on that spot and i'm gonna keep an eye on it. Anyone more experienced think it looks more like a contam?
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whiskeyjack
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] 1
#25642754 - 11/27/18 01:36 PM (5 years, 2 months ago) |
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Hi Folks,
There's a long black thing in my grain jar. It's coming out of a particularly dark oat. I don't think it was there before. It looks like some kind of stroma. Admittedly, I haven't paid much attention to this batch, and it could've been there all along.
Also, I know the moisture is too high in these jars. My mistake. Please don't drag me.
Have you seen anything like this before?
Thanks!

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nube424


Registered: 12/03/17
Posts: 6,063
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Re: [STICKY] Recognizing and dealing with contamination [Re: whiskeyjack] 1
#25642764 - 11/27/18 01:41 PM (5 years, 2 months ago) |
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Could be ergot or just a rotted grain or was overly ripe when they picked it.
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Oatman2000
-=Outa Space=-




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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 1
#25650686 - 12/01/18 01:24 PM (5 years, 2 months ago) |
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I love that you used one of my pics in this.
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Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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fusselchen
Stranger
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Re: Recognizing and dealing with contamination [Re: Oatman2000] 1
#25667330 - 12/09/18 08:15 AM (5 years, 2 months ago) |
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thank you for this helpful sticky post! i'm always curious what kind of contamination there is. a few days ago i had these mushrooms
 and
 when they started looking wet i first thought thats just some water because of my misting and will evaporate. but it didnt and a moderate smell of dirty laundry appeared and i threw them away. i guess it was some kind of bacteria and my misting-mistake (too much? also on the fruit bodys)?
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Shroomspective
Looking into the void



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Re: Recognizing and dealing with contamination [Re: fusselchen] 1
#25667768 - 12/09/18 11:46 AM (5 years, 2 months ago) |
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Bottom one is just a mutant..normal in MS...The white flecks are also pretty standard, but the wet patches likely bacterial infection, probably over misting initially, usually you'd see it on the stipe too
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dmar



Registered: 09/09/18
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Re: Recognizing and dealing with contamination [Re: fusselchen] 1
#25675318 - 12/12/18 06:51 PM (5 years, 2 months ago) |
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Quote:
fusselchen said: thank you for this helpful sticky post! i'm always curious what kind of contamination there is. a few days ago i had these mushrooms
 and
 when they started looking wet i first thought thats just some water because of my misting and will evaporate. but it didnt and a moderate smell of dirty laundry appeared and i threw them away. i guess it was some kind of bacteria and my misting-mistake (too much? also on the fruit bodys)?
I had a MS grow that almost the entire tub was mutants with odd caps like that. Some of them grew with the cap completely inside out, some with the cap almost upside down, some just like yours is there. It's nothing to worry about.
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dmar



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Re: Recognizing and dealing with contamination [Re: dmar] 1
#25683468 - 12/16/18 04:51 PM (5 years, 2 months ago) |
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I'm concerned that this might be trich, but due to my abundance of side pins, I'm pretty sure that my surface conditions were dry. So it could also be bruising from being dry. It's in a few places on the surface of this sub, but it's very faint. If it is trich, how long would it take for it to become more distinguishable? How fast does trich move once it's started dropping spores and turning green?
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Dtwiz
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Re: Recognizing and dealing with contamination [Re: dmar] 1
#25689066 - 12/19/18 09:23 AM (5 years, 1 month ago) |
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This is my first time growing. I have four jars, inoculated with Puerto Rican. One of them is showing two light green patches. What can this be and what can i do?
Thanks in advance
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dmar


Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
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Re: Recognizing and dealing with contamination [Re: Dtwiz] 1
#25689444 - 12/19/18 12:34 PM (5 years, 1 month ago) |
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Quote:
Dtwiz said:


This is my first time growing. I have four jars, inoculated with Puerto Rican. One of them is showing two light green patches. What can this be and what can i do?
Thanks in advance
It's hard to tell with that picture, but if it's green, it's no good. If it's indeed contaminated, pressure cook it at 15 PSI for 90 minutes before you open it to throw it away. If it's kind of a faint color, wait a few days to be sure it's actually green.
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Growgrl



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Re: Recognizing and dealing with contamination [Re: dmar] 1
#25689552 - 12/19/18 01:30 PM (5 years, 1 month ago) |
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Okay, I'm totally frustrated here. Last year I had several successful grows: wbs and coir in monotubs. Life happened and I had to stop growing for a few months. On 11/16/18 I inoculated a bunch of sterile wbs jars with agar. Everything was looking great. They fully colonized and I made monotubs spawning the wbs with coco coir. I should've documented the date but I didn't (probably around 11/27). My mushrooms just aren't pinning. They've been really dry due to the winter air and the only room I have with heat that I can grow in is heated by a wood burning stove. So I've had to mist a lot and still I'm not seeing much condensation on the tubs like I did before. So now I'm thinking they're contaminated. I don't know. Anybody?
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XnMe
Somth'n of a Somth'n



Registered: 06/23/16
Posts: 1,278
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Re: Recognizing and dealing with contamination [Re: Growgrl] 1
#25689858 - 12/19/18 04:15 PM (5 years, 1 month ago) |
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Quote:
Growgrl said: Okay, I'm totally frustrated here. Last year I had several successful grows: wbs and coir in monotubs. Life happened and I had to stop growing for a few months. On 11/16/18 I inoculated a bunch of sterile wbs jars with agar. Everything was looking great. They fully colonized and I made monotubs spawning the wbs with coco coir. I should've documented the date but I didn't (probably around 11/27). My mushrooms just aren't pinning. They've been really dry due to the winter air and the only room I have with heat that I can grow in is heated by a wood burning stove. So I've had to mist a lot and still I'm not seeing much condensation on the tubs like I did before. So now I'm thinking they're contaminated. I don't know. Anybody?
Looks like bruising from pooling water, up FAE and stop misting for now.
Edit: at second look around the poly looks like really healthy myc, definitely up the fae !
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Thanks too all Best Tek List, click it
Edited by XnMe (12/19/18 04:17 PM)
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Recognizing and dealing with contamination [Re: XnMe] 1
#25690255 - 12/19/18 07:27 PM (5 years, 1 month ago) |
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Could be misting too much / too hard also. Just give it a light mist from a few feet above. High pressured mist can bruise mycelium
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Growgrl



Registered: 03/25/18
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Re: Recognizing and dealing with contamination [Re: nube424] 1
#25690744 - 12/20/18 04:52 AM (5 years, 1 month ago) |
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Okay. I'll up the fae and fewer mists from a higher level. Thanks for the replies.
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nube424


Registered: 12/03/17
Posts: 6,063
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Re: Recognizing and dealing with contamination [Re: Growgrl] 1
#25690768 - 12/20/18 05:28 AM (5 years, 1 month ago) |
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Any time chica. When u mist, u want it to look like morning dew on the surface. Just tiny droplets that glisten. U only need to mist if it actually looks dry.
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Growgrl



Registered: 03/25/18
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Re: Recognizing and dealing with contamination [Re: nube424] 1
#25693852 - 12/21/18 01:52 PM (5 years, 1 month ago) |
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Quote:
Okay, I'm totally frustrated here. Last year I had several successful grows: wbs and coir in monotubs. Life happened and I had to stop growing for a few months. On 11/16/18 I inoculated a bunch of sterile wbs jars with agar. Everything was looking great. They fully colonized and I made monotubs spawning the wbs with coco coir. I should've documented the date but I didn't (probably around 11/27). My mushrooms just aren't pinning. They've been really dry due to the winter air and the only room I have with heat that I can grow in is heated by a wood burning stove. So I've had to mist a lot and still I'm not seeing much condensation on the tubs like I did before. So now I'm thinking they're contaminated. I don't know. Anybody?
The morning after I posted this, I woke up to pins! lol
Edited by Growgrl (12/21/18 01:55 PM)
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Octopus8
Aquatic Shape Shifter


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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] 1
#25835850 - 02/24/19 11:18 PM (4 years, 11 months ago) |
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This is like the equivalent of sexual education and seeing all the pictures of STD's.
Great work!
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Blacklight98
Myc hobbyists


Registered: 12/09/18
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Re: Recognizing and dealing with contamination *DELETED* [Re: Octopus8] 1
#25874301 - 03/14/19 05:03 PM (4 years, 10 months ago) |
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Post deleted by Blacklight98
Reason for deletion: Wasnāt edited
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Blacklight98
Myc hobbyists


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Re: Recognizing and dealing with contamination [Re: Blacklight98] 1
#25874355 - 03/14/19 05:34 PM (4 years, 10 months ago) |
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Could anyone tell me if this shroom looks okay there is white fuzzies around all the stocks i read where someone was saying its fine if there is white fuzz on the stock said it was just myc because of lack of air exchange but the discoloring at the bottom of the stem right when i Cropped it and i cut it open an it was white inside dont smell bad
Edited by Blacklight98 (03/14/19 05:39 PM)
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Count of Sabugosa
Nerdy floater



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Re: Recognizing and dealing with contamination [Re: Blacklight98] 1
#25924873 - 04/09/19 11:40 AM (4 years, 10 months ago) |
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These mycobags were inoculated 3 weeks + 2 days ago and showed ZERO growth until last week. I mashed this bag with a couple of spots only because I thought "whatta heck, I've got nothing to lose anymore," since I was about to toss all the other bags. Out of 5, 2 survived. 1 shows a normal pace and healthy growth.
This one is sweating a lot. It's also colonizing faster (perhaps because of the mash?). I am not sure, because I see healthy myc inside, but also a thinner white veil and this to me seems excessive sweating. Thoughts?
-------------------- In Hebrew, the words "wine" and "secret" hold the same numerologic value. When wine comes in, secrets spill out. Do you think the person who said that knew mushrooms? When mushrooms come in... Is there anything beyond a secret?
Edited by Count of Sabugosa (04/09/19 11:41 AM)
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Kizzle
Misanthrope


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It's hard to see what's going on in that bag. I'd give it some time to finish colonizing and check it out then when you can open the bag.
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Count of Sabugosa
Nerdy floater



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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#25931803 - 04/13/19 03:55 AM (4 years, 9 months ago) |
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Here's 4.5 days after 100% break.
-------------------- In Hebrew, the words "wine" and "secret" hold the same numerologic value. When wine comes in, secrets spill out. Do you think the person who said that knew mushrooms? When mushrooms come in... Is there anything beyond a secret?
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Land Trout
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Iāve got a really nice pin set, but trich just showed up. What should I do Cut out the trich and let it flush? Smother the trich in limestone powder? Toss it in my worm bin and let it flush In There?
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Pusynality
Stranger



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Re: Recognizing and dealing with contamination [Re: Land Trout] 1
#25935227 - 04/14/19 10:32 PM (4 years, 9 months ago) |
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So I already threw this jar away when I saw this,

But, what kind of contamination is this and what would have caused it?
I also have 5 other jars next to this one (like a few inches apart on the same table) when I discovered this particular jar. The other jars do not show anything similar, but would they be at risk for anything since they were so close to the contaminated jar (like maybe spread contamination airborne or something)?
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lightrises
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Re: Recognizing and dealing with contamination [Re: Pusynality] 1
#26033231 - 06/04/19 08:33 PM (4 years, 8 months ago) |
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im guessing black mold? ive had a feew jars look exactly like that. I either get rid of the jar or deff isolate it from the others and out of your grow area
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Mycelial Messenger
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] 1
#26038242 - 06/07/19 11:33 AM (4 years, 8 months ago) |
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Great article
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Shroomterd
42



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Very nice write up Iāll definitely be saving this
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Zeak
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Re: Recognizing and dealing with contamination [Re: Shroomterd] 1
#26083857 - 07/01/19 09:36 AM (4 years, 7 months ago) |
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Seems like all of my grain jars have been contaminated. Trying to identify what with exactly and narrow down where could I have introduced it. It's very fluffy and appeared 4 days after ms injections in places of those injections. Rye was boiled for an hour and then PC'd for another hour. Any guesses? š¶

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the_mycologist
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Re: [STICKY] Recognizing and dealing with contamination [Re: Zeak] 1
#26086596 - 07/02/19 04:31 PM (4 years, 7 months ago) |
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My first real grow has been going really well until today when I noticed a couple of blue-ish spots in my tub. Do you think it's mold? If so, can I quarantine those spots somehow or should I go ahead and harvest what mushrooms have already grown and call it a bust?

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FryMe
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Re: [STICKY] Recognizing and dealing with contamination [Re: the_mycologist] 1
#26109935 - 07/15/19 03:47 PM (4 years, 6 months ago) |
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So I've been doing many bulk grows with cubes (B+, Treasure Coast, Golden Teachers, Blue Meanie, Koh Samui Super Strain, Pink Buffalo) and had great success on inoculating the jars in a SAB. Only contams I ever had was trich after many flushes of the tubs, so not big losses.
I decided to try out some Pan Cy's in agar dishes, inoculating directly from a syringe. All the plates I did turned to mold within 3 days. This is my first attempt with agar and my SAB was always nuked prior to working in it and I take many precautions, so I'm a bit confused. I used Bods methods for agar, following to a T and still got contams. Is there anything different with Pans? Should I just inoculate jars instead and then attempt to clone my Pans, since I know I don't get contams direct to jar?
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COGSx86
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Re: [STICKY] Recognizing and dealing with contamination [Re: FryMe] 1
#26129106 - 08/09/19 08:57 PM (4 years, 6 months ago) |
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Is this contamination?
If so, anything I can do, any suggestions?
First tub
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SamAtticus


Registered: 02/27/18
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Re: Recognizing and dealing with contamination [Re: Zeak] 1
#26143012 - 08/19/19 02:41 PM (4 years, 5 months ago) |
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I have the same problem. And the really bizarre part is, if you let it grow, it sprouts these weird-ass mushroom shaped' things.
--------------------
  "I learn by going where I have to go." Theodore Roethke Bod's AF Search Engine Tek
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COGSx86
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Re: Recognizing and dealing with contamination [Re: SamAtticus] 1
#26146496 - 08/21/19 03:05 PM (4 years, 5 months ago) |
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Quote:
SamAtticus said: I have the same problem. And the really bizarre part is, if you let it grow, it sprouts these weird-ass mushroom shaped' things.
sane, ate the mushrooms, and Im still alive
Edited by COGSx86 (08/21/19 03:05 PM)
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Spiritualscience
Researcher


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Re: Recognizing and dealing with contamination [Re: COGSx86] 1
#26150213 - 08/23/19 09:48 PM (4 years, 5 months ago) |
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Man, a guy just shared this and I have never seen anything like it. What in the holy Hell is this???? Looks like some alien bacterial blobs. I just can't put a name to it.
-------------------- Jesus said to the ones who believed Him, If ye continue in my word, you shall know the truth, and the truth shall make you free.
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peanutbutterwaferb
Stranger

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Re: Recognizing and dealing with contamination [Re: Spiritualscience] 1
#26152935 - 08/25/19 09:06 PM (4 years, 5 months ago) |
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Does anyone have a name for this?

Here's another:
Edited by peanutbutterwaferb (08/27/19 09:00 AM)
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ontheinternet
Stranger

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Second monotub grow and got some weird growth. It's almost like there are mushrooms heads growing on the mushroom heads. I don't think it's a contaminate, but not sure.
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Spiritualscience
Researcher


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Re: Recognizing and dealing with contamination [Re: ontheinternet] 1
#26165126 - 09/02/19 10:43 AM (4 years, 5 months ago) |
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Totally normal. It will not hurt anything.
Here is a first flush from a tub. It is on its sixth flush now. Only the first flush had the growths. It could be high humidity, minor response to bacteria, etc. Whatever it is, it has no negative effects on the shrooms.
-------------------- Jesus said to the ones who believed Him, If ye continue in my word, you shall know the truth, and the truth shall make you free.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Recognizing and dealing with contamination [Re: Spiritualscience] 1
#26165128 - 09/02/19 10:46 AM (4 years, 5 months ago) |
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Quote:
Spiritualscience said: Man, a guy just shared this and I have never seen anything like it. What in the holy Hell is this???? Looks like some alien bacterial blobs. I just can't put a name to it.

Just looks like normal SW penis envy blobs. They are common on the first flush.
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Spiritualscience
Researcher


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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 1
#26165157 - 09/02/19 11:07 AM (4 years, 5 months ago) |
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Dang, I think he chunked it already, but I will let him know for sure. He said he put it outside, but that was over a week ago.
Thanks for letting us know!
-------------------- Jesus said to the ones who believed Him, If ye continue in my word, you shall know the truth, and the truth shall make you free.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Recognizing and dealing with contamination [Re: Spiritualscience] 1
#26165185 - 09/02/19 11:24 AM (4 years, 5 months ago) |
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Thatās a shame. Blobs will get some decent size and are just as potent and good to eat.
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ReptilianBrain
Stranger
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Re: Recognizing and dealing with contamination [Re: Pastywhyte] 1
#26165897 - 09/02/19 07:47 PM (4 years, 5 months ago) |
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Would it be cause for concern if Iāve noticed a slight fermentation/ earthy/ musty smell coming off my substrate 7 days into the spawn run? Initially I had the lid gasket sealed for 4 days before finding out that was a mistakeāopened it up and there was a strong fermented smell, but not sure it wasnāt just from having misted H2O2 over the walls and substrate before sealing it up. Now 7 days into spawn run it seems like everything is okay, still notice a slight smell just sort of earthy/mustyānothing crazy. At 4 days, I flipped the lid upside down for FAE, sprayed a bit more H2O2 and have since just been keeping a close eye on everything. Does thi sound okay by what Iāve described? Any suggestions on how to proceed? Iām not quite 100% colonized yet, temps around 75-78, humidity seems good. Did I dodge a bullet or Is it too early to tell? Below is a pictureāseems okay? Anybody see something Iām missing?
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: Recognizing and dealing with contamination [Re: ReptilianBrain] 1
#26169223 - 09/04/19 05:37 PM (4 years, 5 months ago) |
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Quote:
ReptilianBrain said: Would it be cause for concern if Iāve noticed a slight fermentation/ earthy/ musty smell coming off my substrate 7 days into the spawn run? Initially I had the lid gasket sealed for 4 days before finding out that was a mistakeāopened it up and there was a strong fermented smell, but not sure it wasnāt just from having misted H2O2 over the walls and substrate before sealing it up. Now 7 days into spawn run it seems like everything is okay, still notice a slight smell just sort of earthy/mustyānothing crazy. At 4 days, I flipped the lid upside down for FAE, sprayed a bit more H2O2 and have since just been keeping a close eye on everything. Does thi sound okay by what Iāve described? Any suggestions on how to proceed? Iām not quite 100% colonized yet, temps around 75-78, humidity seems good. Did I dodge a bullet or Is it too early to tell? Below is a pictureāseems okay? Anybody see something Iām missing?

The fermentation odor was related to the lack of gas exchange. It's possible for a substrate to recover fully after fixing a problem like that but no guarantees.
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drspastic
the good doctor



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Re: Recognizing and dealing with contamination [Re: Kizzle]
#26171753 - 09/06/19 01:08 AM (4 years, 5 months ago) |
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hi, latest batch of substrate has that sour smell soon after i spawned it. corn spawn very good and clean, straw boiled for over an hour. only difference from last set of monos was i tried packing the layers of spawn and straw into clear plastic sacks stood in a empty mono to make large bricks then putting the bags on shelving.
I have read that gas exchange problems can cause smells but these are sour.
is there any cure or shall I dinv it outdoor to make another garden patch?
bricks are about 5kg each and are heavily spawned
-------------------- did you eat all that acid? you better pray to god there's some thorazine in that bag.
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Motomushy
Stranger

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Re: Recognizing and dealing with contamination [Re: drspastic]
#26191576 - 09/16/19 11:56 AM (4 years, 4 months ago) |
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Can anyone tell me if this is contaminated? The get lighter in color as they grow
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Recognizing and dealing with contamination [Re: Motomushy]
#26192379 - 09/16/19 05:11 PM (4 years, 4 months ago) |
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Quote:
Motomushy said: Can anyone tell me if this is contaminated? The get lighter in color as they grow

Those are too dry. You need another layer of mp tape over the bottom holes. No contams that I can see.
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Motomushy
Stranger

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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
#26192930 - 09/16/19 10:43 PM (4 years, 4 months ago) |
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Thank you! I sprayed a lil water mist in the tub and they have a blueish hue now... That's normal right?
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myc.kind.o.lady


Registered: 09/17/19
Posts: 4
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#26208652 - 09/25/19 01:31 PM (4 years, 4 months ago) |
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Hello all :-) Amazing original post, thanks for all the great info!! MUSH APPRECIATED!!
This is my first time posting anything so I'm apologizing in advance if not proper...
I've been having this issue and i'm losing sleep ...
colonized jars look and smell great - when they are spawned to bulk and after consolidation this is what is happening around day 7-10 after fruiting:


(I could try taking better pics if necessary)
My worst fear is that this is ASPERGILLUS - Can anyone confirm what it is?
**I've been retracing steps and thinking that my pasteurization technique is prob faulty - vac bags filled with bulk sub and cooked in water (170 for 2hrs) i deff over filled and over wet bulk probably causing to get too hot on outside and not enough in center ?? *** I've also had the window open (no AC and super hot in here) could this have been a factor?
if not aspergillus ...Could these blocks be saved? Maybe soaking in mild bleach solution??
I tossed out about 10 (2L) blocks last time as soon as I saw yellow (they were just starting to fruit)....so sad ... but noticed that it only affected the top - under liner everything looked and smelled healthy....
now I have 8 new tubs that are affected and hoping I can save if possible
If not able to save - no problem - health and safety first and also the MAIN thing I need to know is What am I doing wrong and how can I avoid this in the future???
Thanks so much for your time
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flyingcap
Explorer

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Re: Recognizing and dealing with contamination [Re: myc.kind.o.lady]
#26243011 - 10/10/19 03:30 PM (4 years, 4 months ago) |
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Got a few ugly Bettys growing. Reading through some prior threads it can be over misting and lack of FAE or a genetic mutation. Can anyone advise the exact cause and solution for future growing knowledge ? Appreciate the knowledge folks.
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tomcards



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Re: Recognizing and dealing with contamination [Re: flyingcap]
#26289644 - 10/31/19 11:45 AM (4 years, 3 months ago) |
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Thanks to Kizzle for this fantastic, informative thread.
Can anyone identify the beast or beasties that contaminated this tub?
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solarflash
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
#26349508 - 11/27/19 08:44 PM (4 years, 2 months ago) |
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The first 4 times I ever tried the pftek method I was successful. I enjoyed the fruits of my labor. The next 6 times all ended in fast spreading green contamination. The cakes were fully colonized and put into sanitized fruiting chamber. I fanned them daily and used the same care as the first 4 successful batches. Soon after the contamination overtook all the cakes. Batch after batch until I wasted 6 spore syringes. I gave up for a couple of years but am going to try again. This time using a sterilized rye grain kit. Many posters here are very adamant about not using a kit but how bad can these kits be. Whatās worse? Spending $50 on a sterilized kit or wasting syringe after syringe and not knowing exactly where you went wrong? Appreciate any constructive advice.
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ZombiWurm
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Re: Recognizing and dealing with contamination [Re: tomcards]
#26366470 - 12/07/19 08:44 AM (4 years, 2 months ago) |
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Cobweb in the bottom left corner?
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drewman522
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Re: Recognizing and dealing with contamination [Re: ZombiWurm]
#26368810 - 12/08/19 10:48 AM (4 years, 2 months ago) |
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Thank you for this detailed write up! I'm brand new to this forum, apologies if this reply breaks any rules. And sorry to ask what may be the obvious but hoping someone more experienced than me can confirm that this cake is contaminated and should just be trashed? OR is it worth continuing?
This is our first grow and we've been following the PF Tek Easy BRF Cakes found here: https://www.shroomology.org/forums/topic/13173-pf-tek-easy-brf-cakes-mushroom-growing-method/
Started on 11/3 with 8 cakes and already had to dispose of 6 of them because of visible contams and really no white mycelium.
Any guidance is greatly appreciated!
https://imgur.com/bP32ldr
Edited by drewman522 (12/08/19 10:50 AM)
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ZombiWurm
Stranger

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Re: Recognizing and dealing with contamination [Re: drewman522]
#26368828 - 12/08/19 11:00 AM (4 years, 2 months ago) |
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That's the wrong jar for BRF cakes. You won't ever get the cake out of that jar. Anyway it looks fine to me. Stick to wide mouth half pint jars. I've always used the regular mouth half pints but it seems the short ones colonize faster
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Psilotyl
× Öø×Ö“×ר


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Re: Recognizing and dealing with contamination [Re: tomcards]
#26368935 - 12/08/19 12:05 PM (4 years, 2 months ago) |
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Quote:
tomcards said: Thanks to Kizzle for this fantastic, informative thread.
Can anyone identify the beast or beasties that contaminated this tub?

hard too really see there. Even so...who knows :/ I donāt think itās penicillium. It could be aspergillus, chaetomium, trichoderma, maybe cladosporium...but clad is usually darker looking than whatās in your pic. Sadly there are so many āgreensā that can eff shit up itās hard for most of us to say whatās what with certainty
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ZombiWurm
Stranger

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Re: Recognizing and dealing with contamination [Re: Psilotyl]
#26369350 - 12/08/19 04:02 PM (4 years, 2 months ago) |
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Quote:
Psilotyl said:
Quote:
tomcards said: Thanks to Kizzle for this fantastic, informative thread.
Can anyone identify the beast or beasties that contaminated this tub?

hard too really see there. Even so...who knows :/ I donāt think itās penicillium. It could be aspergillus, chaetomium, trichoderma, maybe cladosporium...but clad is usually darker looking than whatās in your pic. Sadly there are so many āgreensā that can eff shit up itās hard for most of us to say whatās what with certainty
Yeah it's really hard to tell. You could culture it but even then without a scope you are stuck with observing how it colonized the agar to determine what it is and how the mycelium grows.
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tomcards



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Re: Recognizing and dealing with contamination [Re: ZombiWurm]
#26370574 - 12/09/19 09:50 AM (4 years, 2 months ago) |
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Thanks for the feedback.
My sterile technique was sorely lacking. There were probably all manner of nasty beasties in the substrate. After one flush, I harvested 83.3 cracker-dry grams of Golden Teacher. I dumped the rest of the substrate. I kick myself as I fantasize about how much that tub could have yielded.
Since then, I've learned much. This won't happen again.
Tom
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drewman522
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Re: Recognizing and dealing with contamination [Re: ZombiWurm]
#26370733 - 12/09/19 11:19 AM (4 years, 2 months ago) |
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Realized I replied to the wrong post, but thanks for the reply! Wow so you think no concerns with contams based on those pics?
Thanks for the note on the jars - these are wide mouth as directed but realized too late exactly what you're saying... may end up having to break them to get the cakes out.
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Psilotyl
× Öø×Ö“×ר


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Re: Recognizing and dealing with contamination [Re: tomcards]
#26370877 - 12/09/19 12:49 PM (4 years, 2 months ago) |
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Quote:
tomcards said: Thanks for the feedback.
My sterile technique was sorely lacking. There were probably all manner of nasty beasties in the substrate. After one flush, I harvested 83.3 cracker-dry grams of Golden Teacher. I dumped the rest of the substrate. I kick myself as I fantasize about how much that tub could have yielded.
Since then, I've learned much. This won't happen again.
Tom
Thatās still not bad! Thereās always next time, as lame as that sounds lol
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A_Fun_Guy_Learns
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Re: Recognizing and dealing with contamination [Re: Psilotyl]
#26372091 - 12/09/19 11:54 PM (4 years, 2 months ago) |
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Is this the meany greeny, or just metabolites?
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Psilotyl
× Öø×Ö“×ר


Registered: 08/30/19
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Loc: ×¢×××
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Quote:
A_Fun_Guy_Learns said: Is this the meany greeny, or just metabolites?

Looks like thatās probably fine. Keep an eye on it and see if it turns & youāll get a definitive answer pretty quickly
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MushToLearn
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Re: Recognizing and dealing with contamination [Re: Psilotyl]
#26418832 - 01/06/20 07:06 AM (4 years, 1 month ago) |
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Hi folks, newbie here. I've just managed my first grow of 5 BRF cakes. I've done one flush so far and have dunked and setup for flush No.2. Past day or so i've noticed a very slight musky smell coming from my FC which made me head here to try get some info on possible contams. After reading a lil i am leaning towards a possible Trich contam but im not sure as i obviously lack the experience. I can still smell the Mycelium as on the first flush but now with a very slight musky smell. I don't see any obvious mold spots but after reading about the white growth that comes before Trich spores i have a feeling this is what i am faced with. If it wasn't for the slight smell i would have thought these cakes were fine. Posted some pics so if anyone would like to help out a newbie i'd appreciate the advice. Cheers.




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Kizzle
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Re: Recognizing and dealing with contamination [Re: MushToLearn]
#26419784 - 01/06/20 06:19 PM (4 years, 1 month ago) |
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All you can do is wait and see.
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drewman522
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#26422048 - 01/07/20 09:47 PM (4 years, 1 month ago) |
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Hi all, thanks to @zombiwurm for replying to my last post. Have some updated photos (and figured out how to include images properly ) so I though i'd post again. These BRF cakes are just over 2 months old and i think looking really good except for a few possible contams in each. Can anyone help identify and is there any cause for concern or should we proceed with the dunk and fruiting?

Thank you!
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snowytips
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
#26453014 - 01/26/20 06:47 AM (4 years, 21 days ago) |
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After a couple of weeks or so of mushrooms growing on the outside of the casing, however on the top where no mushrooms are growing some green mold has started to appear... any suggestions of what it is or to remedy the situation?? 
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D123456
Stranger

Registered: 01/28/20
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Please can anyone help me, does anyone know if my crop is contaminated
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Tynuk
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Hi everyone, Was hoping if someone could give me an advise. I have 2 spots of dark green mold in my monotub (wbs and coco coir). I hace read on here that I can try to cut them out and cover the spots with salt. Anyone tried doing that before?
Thanks in advance!

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killmatrixolme
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#26480287 - 02/11/20 02:14 PM (4 years, 5 days ago) |
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Can i eat this mushroom? i think the white color is bold. they are contaminated right?
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SynKyd
ctrl-alt-delite



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Re: Recognizing and dealing with contamination [Re: killmatrixolme]
#26480980 - 02/11/20 08:04 PM (4 years, 5 days ago) |
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Quote:
killmatrixolme said: Can i eat this mushroom? i think the white color is bold. they are contaminated right?

Those are fine. Scrape the verm off the bottom and enjoy
-------------------- New inclusive poop emojis from Apple!
   
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endlessnessagain
Stranger
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Re: Recognizing and dealing with contamination [Re: SynKyd]
#26483155 - 02/13/20 06:01 AM (4 years, 3 days ago) |
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Is there anything like this thread but specifically for agar contamination, to help identify them?
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Nigel Stephens

Registered: 02/21/20
Posts: 103
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Do I have anything to worry about here, are they safe to eat? I think itās pseudomonas or verticillium, stems are hollow too
This is 2nd flush after dunking substrate / floating harvest tek. Have since removed lid for FAE and drying but should I just harvest and toss? Obvs then bleach my whole grow area 
Thank-you in advance for your time and advice


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Kizzle
Misanthrope


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Re: Recognizing and dealing with contamination [Re: Nigel Stephens]
#26503268 - 02/25/20 02:09 PM (3 years, 11 months ago) |
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They're still edible. It looks like at an earlier time they were drenched and got waterlogged.
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RyeHumor
MasterProcrastinator



Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
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2 out of 18 BRF Jars... So long Fare Well? [Re: Kizzle]
#26503422 - 02/25/20 03:33 PM (3 years, 11 months ago) |
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Howdy
To give a background on the grow started 2-16:
Purchased a grow kit online, "pre-sterilized" jars and all. Coupled with my poor technique looks like of the 18 pint jar BRFs: (3)nonstarters (1)definitely contam'd and thrown out (green mold) (12)looking good (2)I suspect contam and have isolated.
Here are the two in question: The first was a result of a slipped plunger that colonized half the jar almost within the first week. Looked good until sectioning appeared, the second growth looks white if not as bright as the first portion. What really bugs me is that there appears to be no rhizomorphic growth at the edges to match the brighter section. Here are some photos of that jar. The black marks are in the 3rd photo are left over from pen marks I was using to track progress.
   
And here is the second jar in question:
 
I'm 95% sure that these two are ready to be tossed, however I would love to hear any thoughts to the contrary as well as a possible identity of the contamination if anyone can provide. Thank you!
Edited by RyeHumor (02/25/20 06:30 PM)
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Funjunk1121
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Re: Recognizing and dealing with contamination [Re: RyeHumor]
#26509979 - 02/29/20 04:22 PM (3 years, 11 months ago) |
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Hey what are your thoughts on what I have going on here?
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Funjunk1121
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Re: Recognizing and dealing with contamination [Re: Funjunk1121]
#26510147 - 02/29/20 05:59 PM (3 years, 11 months ago) |
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beammeup90
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
#26520965 - 03/06/20 04:59 PM (3 years, 11 months ago) |
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How bad is the contamination and is it a toss the whole tub situation?
Higher res https://files.shroomery.org/files/20-10/355223956-QdaqgFl.jpg
This is the original grow:[image]Original https://imgur.com/a/BCOGcnf[/image]
This is the current grow that I think may be contaminated. Any thoughts on the difference in size and shape? https://files.shroomery.org/files/20-10/363943527-IMG_20200307_180811.jpg
Edited by beammeup90 (03/07/20 08:52 PM)
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KookaburraMan
not a jock



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Re: [STICKY] Recognizing and dealing with contamination [Re: beammeup90]
#26525452 - 03/09/20 12:52 PM (3 years, 11 months ago) |
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Hey shroomers! I think these are pre-pin bumps but as a second-time grower, I just wanna make sure everything is honky dory. Thanks for reviewing and possibly giving me some feedback! Love y'all!

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Cowboy Buckie
General


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Re: Recognizing and dealing with contamination [Re: tomcards]
#26528840 - 03/11/20 09:52 AM (3 years, 11 months ago) |
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I assume youāre talking about the cpl green spots? How much has it changed since you posted?
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beammeup90
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Re: Recognizing and dealing with contamination [Re: Cowboy Buckie]
#26530127 - 03/12/20 12:18 AM (3 years, 11 months ago) |
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The color didn' change much. Primarily white with a little yellow, brown, and a tiny bit of green. I harvested the first flush today. It may have been a bit early but i had a mix of buttons to fully developed mushrooms. What's most interesting is how they differ from the grow that the spores came from. The prior grow had Longer skinny stalks and relatively small caps. This grow is filled with short stocky robust mushrooms. [url=http://files.shoomery.org/forums/thumbs/20-11/399103535-thumb_MG_0263.jpg[/image][/url] [url=http://files.shoomery.org/forums/thumbs/20-11/399104362-thumb_IMG_20200311-113634.jpg[/image]
-------------------- I've had poison oak every month for five years
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LREAM
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Re: Recognizing and dealing with contamination [Re: beammeup90]
#26534789 - 03/14/20 01:00 PM (3 years, 10 months ago) |
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Towards the bottom right corner I have a blue discoloration. Its a bit hard to tell since I just noticed this morning. This is my first grow and I just pushed my first fruting bin in a few days ago. Want to know if I should trash and start over or if I'm dealing with something other than blue mold

In front of the pin

It does seem a bit wet on the surface so I cracked the lid more for FAE. If anyone can help identify or has any suggestions that would be great, before I end up trashing it if it gets worse. Thanks!
Edited by LREAM (03/14/20 01:03 PM)
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squidward18
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
#26535466 - 03/14/20 08:11 PM (3 years, 10 months ago) |
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Any ideas on what this might be? Looks like there's a little pool of gunk.

 Some of the stocks have developed these bubbles:
 Thanks in advance for any help!
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Uncaged_Goose
Stranger
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Re: [STICKY] Recognizing and dealing with contamination [Re: squidward18]
#26563145 - 03/28/20 12:42 PM (3 years, 10 months ago) |
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Long time lurker, first time poster! I'm in the middle of my first ever grow and I'm worried about contamination.
I definitely made some mistakes - 7 out of 10 jars were possibly contaminated so I only fruited 3 of them. The grow looked good but one of my mushrooms was a little slimy under the cap. All the veils were broken, but it didn't seem like they dropped spores yet.
There is some blue that looks like it could be mold on the dirt on top of my cakes (they didn't quite colonize fully). Pictures for everything here: http://imgur.com/gallery/2xhk6Ai
Is this OK? Should I abort and try again? Thank you in advance
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cronicr



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Re: [STICKY] Recognizing and dealing with contamination [Re: Uncaged_Goose]
#26563272 - 03/28/20 01:41 PM (3 years, 10 months ago) |
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the fruits are fine I'm not sure if that's mold or bruising, if it's green it's contaminated
--------------------
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Elusively
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Re: [STICKY] Recognizing and dealing with contamination [Re: cronicr]
#26563896 - 03/28/20 06:20 PM (3 years, 10 months ago) |
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Using pastyās easy agar tek ā> bods easy oat prep tek
I feel like this look good, but they were a little fluffy in the glad mini rounds and still look kinda fluffy.
Anyone have some time to reassure my fragile ego?! Btw, thank in advance for the abundance of info on this site/community, yāall are Effin amazing!
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jbgtaa
extraterrestrial



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Re: [STICKY] Recognizing and dealing with contamination [Re: Elusively]
#26564432 - 03/29/20 01:47 AM (3 years, 10 months ago) |
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Does look a lil fluffy but youāre gonna need a week or two more to really tell unless itās really tams out. How long did that take to grow and how many transfers away from the (I assume) spore plate was the culture you use for inoculation?
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Elusively
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Re: [STICKY] Recognizing and dealing with contamination [Re: jbgtaa]
#26564749 - 03/29/20 08:17 AM (3 years, 10 months ago) |
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Thanks for the reply sir. This is my first time w/ agar and a bulk tek, so Iām just feeling it out, doing sort of a ātrial runā to see how my technique is and what kind of learning curve Iām working on. Just giving you some context before I answer your questions:
-that growth is about 5-6 days after innoc those grain jars.
-I used about 1/10th of a wedge in each jar from what I (very noobishly and very arbitrarily) decided was the healthiest of the 4 agar plates I had.
-I did zero transfers, thatās my first go at agar and like I said, I arbitrarily decided that one looked healthy so eff it letās keep rolling, was my thought process.
-I still have the 3 other agar plates, theyāre about 3.5 weeks old now and the growth appears to have stopped, could I still do a transfer with those?
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Inthepit
Aum Mani Padme Hum



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Re: [STICKY] Recognizing and dealing with contamination [Re: Elusively] 1
#26568473 - 03/31/20 05:40 AM (3 years, 10 months ago) |
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Thank for this awesome reference and helpful place! I could use some help to correct whatever I did to get this black mold. My first guess is the from the water I was misting with. Possibly I could avoid the black mold by misting with bleach?
  A little history of the tubs; first they were too wet, haven't dialed in the proportions yet. Then I took the lids and put them sideways, but I believe the tubs got too dry. Then I started misting heavily. So basically over correction all around, noob style!
This looks like mold. Black Mold? LOL My question is if I caught it early would this How to Make 150 ppm Chlorine Solution by c10 to get rid of it. And I wonder how I got it, there's lots of mold here in the tropics. No one drinks the tap water here!
Edited by Inthepit (03/31/20 07:15 AM)
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Bikerfool
Your Local Edgelord


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Re: [STICKY] Recognizing and dealing with contamination [Re: Inthepit]
#26570251 - 04/01/20 12:36 AM (3 years, 10 months ago) |
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Inthepit, there's no saving that, throw it away ASAP.
If I ever find any contams it goes instantly into the trash. Even a tiny speck of green mold, I'll pick what's there and throw it out. It's not worth "trying to get one more flush." Trace back through your sterile technique and change or eliminate suspect practices.
My #1 piece of advice to a new grower is to get a flow hood.
-------------------- Just an angsty teen contributing to the pubs decline with contentless posts.
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Inthepit
Aum Mani Padme Hum



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Re: [STICKY] Recognizing and dealing with contamination [Re: Bikerfool]
#26570390 - 04/01/20 04:48 AM (3 years, 10 months ago) |
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Quote:
Bikerfool said: Inthepit, there's no saving that, throw it away ASAP.
If I ever find any contams it goes instantly into the trash. Even a tiny speck of green mold, I'll pick what's there and throw it out. It's not worth "trying to get one more flush." Trace back through your sterile technique and change or eliminate suspect practices.
My #1 piece of advice to a new grower is to get a flow hood.
Thankyou for the reply. I wonder if I should use bleach mist anyway as this area has lots of mold.
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Galactic_Grape
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Re: [STICKY] Recognizing and dealing with contamination [Re: Inthepit] 1
#26570923 - 04/01/20 10:57 AM (3 years, 10 months ago) |
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This is day 8 of fruiting, no pins yet. Pf classic with wbs spawn / damion 50/50 coir tek. Woke up this morning and saw these fluffy little balls but I cannot tell if these are knots or the start of trich. Separated this tub from the rest. Iāll know if it goes green by tomorrow but looking for opinions / might be useful to someone down the road if it turns out to be trich so you can see what it looks like before it goes totally green. Spot in question is in the middle of the tub above the crack.
Crack in the sub is from the bottom of the tub bowing down when I had them stacked and then pressed up flat when I put it on the ground.
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Bikerfool
Your Local Edgelord


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Re: [STICKY] Recognizing and dealing with contamination [Re: Inthepit]
#26571048 - 04/01/20 11:58 AM (3 years, 10 months ago) |
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Quote:
Inthepit said:
Quote:
Bikerfool said: Inthepit, there's no saving that, throw it away ASAP.
If I ever find any contams it goes instantly into the trash. Even a tiny speck of green mold, I'll pick what's there and throw it out. It's not worth "trying to get one more flush." Trace back through your sterile technique and change or eliminate suspect practices.
My #1 piece of advice to a new grower is to get a flow hood.
Thankyou for the reply. I wonder if I should use bleach mist anyway as this area has lots of mold.

I wouldn't recommend spraying your grow with bleach even if it is a weak solution. If will kill the mushrooms along with all other types of mold.
Having a dirty/ moldy environment is a disadvantage, but if you have good sterile technique and FAE you'll still be successful.
-------------------- Just an angsty teen contributing to the pubs decline with contentless posts.
Edited by Bikerfool (04/01/20 12:15 PM)
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judenfisch
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Re: [STICKY] Recognizing and dealing with contamination [Re: Bikerfool] 1
#26576664 - 04/04/20 03:57 AM (3 years, 10 months ago) |
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hello everyone!
the mycelium on the stem of the tallest mushrooms looks strange to me.
the greenish/ blueish spot i was afraid of first is bruising i think...
what do u think?? is it contaminated there? i am very afraid, would be nice to have another opinion!
-------------------- everything is information
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murderlabz
RIP Stoneman


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Re: [STICKY] Recognizing and dealing with contamination [Re: judenfisch] 1
#26578245 - 04/04/20 10:18 PM (3 years, 10 months ago) |
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Quote:
judenfisch said: hello everyone!
the mycelium on the stem of the tallest mushrooms looks strange to me.
the greenish/ blueish spot i was afraid of first is bruising i think...
what do u think?? is it contaminated there? i am very afraid, would be nice to have another opinion!

Hard to see, I have had deep greenish bruising before that freaked me out. Do you have a "sterile" Q-Tip? Swab it, if it transfer to the Q-Tip it's spores from a mould.
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artkng
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Re: [STICKY] Recognizing and dealing with contamination [Re: murderlabz] 1
#26580457 - 04/05/20 08:45 PM (3 years, 10 months ago) |
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Lots of great info being shared, much needed for new growers. Thanks for the wisdom!
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Goombala
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Re: [STICKY] Recognizing and dealing with contamination [Re: artkng]
#26581542 - 04/06/20 11:52 AM (3 years, 10 months ago) |
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Hey guys does this look sketchy? It has a smell like bread dough which is the only thing scaring me so far.
 Spawned on 3/30
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R3dstaffy
š¤



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Re: [STICKY] Recognizing and dealing with contamination [Re: Goombala]
#26582170 - 04/06/20 05:01 PM (3 years, 10 months ago) |
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.
Edited by R3dstaffy (07/23/20 04:13 PM)
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coAsTal
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Re: [STICKY] Recognizing and dealing with contamination [Re: R3dstaffy]
#26585900 - 04/08/20 09:04 AM (3 years, 10 months ago) |
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FML-- I just dropped a quart jar full of sporulating trich in my kitchen, releasing a cloud of goddamn spores into the air-- how fucked am i?
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats Spore Trading List
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RyeHumor
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Re: [STICKY] Recognizing and dealing with contamination [Re: R3dstaffy] 1
#26586892 - 04/08/20 05:19 PM (3 years, 10 months ago) |
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With the exception of that one black spot in the middle photo I would say you're looking fine.
I just birthed my pf jars a week ago and the initial growth looked about like that 2-3 days after noc.
any ideas as to what that black portion in the middle jar is?
If you can get in closer on it, might help.
I was in a similar situation with my jars. I obsessed over it, but really unless you are in a hurry to reuse the jar there's not much you can do other than watch it colonize and see if it expresses obvious signs of contamination.
Edited by RyeHumor (07/26/20 11:00 PM)
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RyeHumor
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Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
#26586902 - 04/08/20 05:24 PM (3 years, 10 months ago) |
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Quote:
coAsTal said:
FML-- I just dropped a quart jar full of sporulating trich in my kitchen, releasing a cloud of goddamn spores into the air-- how fucked am i?
Might want to wipe down the area/vacuum, especially if you have a hepa filter in your unit. I don't think there's much more you can do.
I was just reading a thread with some input from Bhod and Mad that seemed on a similar subject (cleaning contaminated tubs).
It seems the best thing you can do regardless is just to make damn sure that your spawn is clean AF.
For your future projects, as long as your spawn is fully colonized and contam free, the rest of the process is not sterile and the myc should be strong enough to resist contamination at that point.
So despair not 
I've heard a lot about bleach bombing places, from what I've gathered on this site from more experienced posters is that this is really not necessary. Probably more like soapy water washdown and vacuuming.
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R3dstaffy
š¤



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Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
#26587300 - 04/08/20 08:38 PM (3 years, 10 months ago) |
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.
Edited by R3dstaffy (07/23/20 04:14 PM)
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onoff
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Re: [STICKY] Recognizing and dealing with contamination [Re: R3dstaffy]
#26588833 - 04/09/20 01:07 PM (3 years, 10 months ago) |
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Thanks for the information everyone  Another first-timer here. Do you think this may be in vitro pinning or metabolites? Not sure whether I should fruit these or not, any opinions welcomed! Thanks.
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coAsTal
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Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
#26589036 - 04/09/20 02:56 PM (3 years, 10 months ago) |
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Thank you for the kind words- - I let the area settle for a couple hours then sprayed/misted the area with cleaner, let is get wet, then cleaned it up. I've already had two months of contamination issues, which is why I was so bummed by this, so maybe in my old age I'm no longer able to properly make clean spawn and am destined to swear off mushrooms forever 
Onward and upward!
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats Spore Trading List
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DezGWest7
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Re: [STICKY] Recognizing and dealing with contamination [Re: onoff]
#26589537 - 04/09/20 07:26 PM (3 years, 10 months ago) |
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What's up guys. First timer here. I inoculated WBS March 27 in 3 Jars with GT. The thing is, i see NO activity in 2 of them and the one that I do see activity, I believe its probably contaminated but I'm not so certain. Here is a pic of it. I left it alone originally for the 1st 9 days or so until i decided to check it out and saw what the picture shows but smaller and yes, growth in those 2 different spots. I'm not sure where I f'ed up but *note- this WBS preparation did not include coffee grounds or gypsum. I rinsed , picked the floating grain off, rinsed until clear - soaked 20+ hours - simmered stovetop - then strained , spread out and left to dry a little over 2 hours(I recall it wasn't 100% dry when I decided to pack in the Jar for PS 120min Sterilization. Was it wet wet ? Noo. It just had enough moisture for me to notice when the grains were touching the inside of the jar after loading up. After the PC I allowed it to cool down more than 16 hours from my recollection & then I did the whole sterilize GT syringe etc inoculation of jars.




Feedback is appreciated.
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folder52
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Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
#26593587 - 04/11/20 01:55 PM (3 years, 10 months ago) |
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Hi everyone! So I got my first 4 cakes recently. There are those green-blue spots everywhere (including pins). It was here since colonization process. Is that contamination? Should I burn my entire house now?



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RyeHumor
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Re: [STICKY] Recognizing and dealing with contamination [Re: folder52]
#26593700 - 04/11/20 03:01 PM (3 years, 10 months ago) |
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Quote:
folder52 said: Hi everyone! So I got my first 4 cakes recently. There are those green-blue spots everywhere (including pins). It was here since colonization process. Is that contamination? Should I burn my entire house now?




I'm sure someone more experienced will chime in. But to me it appears like bruising, which is not something to worry about.
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Crackatoa
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Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
#26594188 - 04/11/20 08:16 PM (3 years, 10 months ago) |
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Big_Dub
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Re: [STICKY] Recognizing and dealing with contamination [Re: Crackatoa]
#26596648 - 04/12/20 08:34 PM (3 years, 9 months ago) |
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Yeah. Looks like bruising from where you have been handling it
-------------------- split_by_nine said: click me you fuck do the right thing
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coAsTal
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Re: [STICKY] Recognizing and dealing with contamination [Re: Big_Dub]
#26599833 - 04/14/20 08:45 AM (3 years, 9 months ago) |
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So a question fellas--- (sorry for the long post, but I'm really stumped.)
I've had some limited results with a 50/50 blend of hydrated straw pellet matter and fine verm in half-pint jars with both SAT cubes and ATL#7 colonizing well with no evident contams. I also have a Zapotecorum pint WBS that appears not contammed that has colonized 100%. 
BUT-- I've had contam issues in all other pints of straw mix, WBS, and barley I've done (a dozen at least), and 100% contamination in all quart-sized jars of straw mix, Barley, and also WBS.
It's been driving me crazy to figure out what the hell is going on, because I have been using a SAB and making sure my agar wedges were good-- but I've had over a dozen-- probably closer to 20 jars actually-- contam with trich in the past 2 months.
I PC'd these jars for no less than 90 minutes after full-heat pre-steaming for 10 to 15 minutes prior to putting the weight on my pressure cooker to get all the dry air out. There are no leaks from the lid.
Initially I thought I must be introducing bacteria into the jars when nocking up, but something just wasn't right, because I have been going nuts to keep things clean w/liberal alcohol and proper SAB methods.
So I decided to PC some jars of the straw and verm mix, and some barley, and just keep them sealed straight out of the PC (with foil on still) set aside to see if somehow I wasn't getting full sterilization.
Well, the results are in: Every jar has contaminated within 2 weeks despite being PC'd and never opened.
How the fuck could this happen?!
I'm using a very small PC-- it'll only fit 2 quarts in it angled on their sides-- and I had a quarter taped to the weight to help weigh it down some. I wait until it has cooled to open and remove the lid. I have the lid holes covered with micropore tape. But somehow, despite the weight shaking a bit indicating full inside pressure, I'm clearly not sterilizing these quarts even with 2 hours under pressure.
I can only surmise that the temps/pressures aren't getting to the levels they need to be at to sterilize the quarts.
So now that I've wasted 10 weeks watching all my efforts fail, can you please help me figure out why I'm failing-- I should have been sitting on ounces of dried shrooms by now, and instead I have 4 half-pint straw jars colonized with mycelium, and one pint of Zap.
I'm making some more straw mix now, and will put two additional quarters on the weight to further increase pressure and will try to make sure I hit 120 minutes at full pressure as a start-- but otherwise I'm not sure what to do...
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats Spore Trading List
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coAsTal
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Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
#26602939 - 04/15/20 01:01 PM (3 years, 9 months ago) |
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I added the extra quarters and PC's for 1:45 hr yesterday-- will nock up with existing SAT half-pint that's 100% colonized on Friday-- I'm hoping the addition of the weight will have increased pressure and heat to fully sterilize the two qurts of pellet straw/verm.
Will update
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats Spore Trading List
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RyeHumor
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Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
#26603006 - 04/15/20 01:26 PM (3 years, 9 months ago) |
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Just a thought. You might benefit from buying some of those autoclave indicators.
I believe the sponsors (mushroomsupply) have both polymer pellets in glass and the autoclave tape.
It indicates when sterilization temperatures have been reached.
Not sure if they take into account the amount of time spent at temp but if it does that could be really useful.
As I've seen mentioned before, venting your pc for ~10min to make sure you have nothing but steam in there is also important.
If you have a mixture of air and water you will not be reaching 121C even if you're up to pressure.
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coAsTal
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Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
#26603278 - 04/15/20 03:20 PM (3 years, 9 months ago) |
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I don't put the lid on until it's already at full boil, then I always vent at full heat and full steam (after the lid lock has popped up) for 10 to 15 minutes with the lid on before putting the primary weight on the valve--
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats Spore Trading List
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RyeHumor
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Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
#26603304 - 04/15/20 03:37 PM (3 years, 9 months ago) |
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Given that you were seeing contamination inside the pc without opening the lid...
I would guess that either it wasn't reaching the pressure you thought it was.
In which case you can just add weight the way you described (but at a certain point you don't want to exceed the limitations of your pc)
Alternatively you can try and confirm with one of those indicators.
and third, it would be nice to have a pressure gauge... but on second thought modifying a pressure vessel is not the safest of ideas. So I can't actually recommend it.
Is $80 for a 23qt Presto PC with a pressure gauge outside of the budget?
I know it's on the more expensive side for this hobby, but I just picked one up and it's been great so far.
----------------
Another thought, what are you using to filter the gas exchange?
Edited by RyeHumor (04/15/20 03:37 PM)
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coAsTal
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Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
#26603353 - 04/15/20 04:02 PM (3 years, 9 months ago) |
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I've been laid off because of this stupid fucking virus bullshit, and have no spare money to use on this hobby (without my wife castrating me)-- so upgrading equipment is a 0% chance for the foreseeable future I'm afraid. 
The three quarters on the weight didn't pop the overpressure valve on the lid, so I think it's OK-- still astonished that I was contamming straight from the PC-- I'm looking forward to these two to see if they perform better with the weights on.
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats Spore Trading List
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RyeHumor
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Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
#26603399 - 04/15/20 04:27 PM (3 years, 9 months ago) |
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Roger that. And sorry to hear it man.
One more exp you could try is to make a no pour and put something obviously contaminated in it to see if it still grows after.
But that's not necessarily that great due to the fact that spores and bacteria can be more or less resistant.
If it fails, it would tell you that something was up for sure though.
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coAsTal
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Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
#26603450 - 04/15/20 04:47 PM (3 years, 9 months ago) |
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No worries-- I appreciate the advice. REEEEALLLY hoping I can get this shit figured out so I can get some shit growing
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RyeHumor
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Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
#26603505 - 04/15/20 05:17 PM (3 years, 9 months ago) |
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yeah I know the feeling.
dealing with contamination aint no fun.
Hang in there man,
if at first you fail, try again.
if you fail again, go office space on the son of a
Just kidding. You'll get there
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folder52
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Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
#26604993 - 04/16/20 06:56 AM (3 years, 9 months ago) |
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An update for my previous post: green-blue something is still here, also some mutants How are we doing?




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poisoned
untitled



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Re: [STICKY] Recognizing and dealing with contamination [Re: folder52]
#26605246 - 04/16/20 08:54 AM (3 years, 9 months ago) |
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Looks dry
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folder52
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Re: [STICKY] Recognizing and dealing with contamination [Re: poisoned]
#26605512 - 04/16/20 10:43 AM (3 years, 9 months ago) |
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Quote:
poisoned said: Looks dry
Is it OK to eat?
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poisoned
untitled



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Re: [STICKY] Recognizing and dealing with contamination [Re: folder52]
#26605657 - 04/16/20 11:31 AM (3 years, 9 months ago) |
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Most definitely!
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mxmushroom
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Re: Recognizing and dealing with contamination [Re: poisoned]
#26605728 - 04/16/20 12:08 PM (3 years, 9 months ago) |
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Hey deadheads! Just harvested a batch of p. cubensis, and sadly my batch has a bad case of cottonweb mildew. Are these still edible? There are no unusual smells or colors, and none of the mushies have been altered in any way except some small amounts of the mildew on the stems. Probably going to eat one and see what happens. I'd love some advice though! I'm a first time grower! Cheers all
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coAsTal
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Re: Recognizing and dealing with contamination [Re: mxmushroom]
#26605746 - 04/16/20 12:19 PM (3 years, 9 months ago) |
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Welcome to the Shroomery-- I'd just take a toothbrush to it to scrub off the funk, but I can't imagine it would be a problem
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats Spore Trading List
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poisoned
untitled



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Re: Recognizing and dealing with contamination [Re: coAsTal]
#26605896 - 04/16/20 01:41 PM (3 years, 9 months ago) |
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Can you show us some pics? Sounds like myc to me
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Evolvedr79
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Re: Recognizing and dealing with contamination [Re: poisoned]
#26610360 - 04/18/20 12:13 PM (3 years, 9 months ago) |
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Hello everyone. This tub is one of three that came from a WBS spawn. Substrate is CVG. The other two tubs produced 2500 wet grams with a 12.5:1 wet to dry ratio. This last tub looks to have metabolites to me which would indicate a contamination but I am not sure which it is. I've only ever dealt with Trich in my previous tubs. The yellow coloring is abnormal to me.
Can someone please tell me if this tub is contaminated and if so, what is it?
Thanks.

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DezGWest7
JacQ


Registered: 04/09/20
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Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
#26610619 - 04/18/20 01:52 PM (3 years, 9 months ago) |
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Quote:
DezGWest7 said: What's up guys. First timer here. I inoculated WBS March 27 in 3 Jars with GT. The thing is, i see NO activity in 2 of them and the one that I do see activity, I believe its probably contaminated but I'm not so certain. Here is a pic of it. I left it alone originally for the 1st 9 days or so until i decided to check it out and saw what the picture shows but smaller and yes, growth in those 2 different spots. I'm not sure where I f'ed up but *note- this WBS preparation did not include coffee grounds or gypsum. I rinsed , picked the floating grain off, rinsed until clear - soaked 20+ hours - simmered stovetop - then strained , spread out and left to dry a little over 2 hours(I recall it wasn't 100% dry when I decided to pack in the Jar for PS 120min Sterilization. Was it wet wet ? Noo. It just had enough moisture for me to notice when the grains were touching the inside of the jar after loading up. After the PC I allowed it to cool down more than 16 hours from my recollection & then I did the whole sterilize GT syringe etc inoculation of jars.




Feedback is appreciated.
Since no one gave me any feedback I waited to see how it would progress and I suppose it answered any questions I had. This is how it looks as of today
Edited by DezGWest7 (04/18/20 01:53 PM)
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WillieFunGuy
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Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
#26622479 - 04/23/20 02:12 PM (3 years, 9 months ago) |
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Dude...looks perfect to me. I understand your trepidation though. Iām fairly new myself. Contams happen to the best of us. Keep it moving. Good job!
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Crackatoa
Stranger in a strange land



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Re: [STICKY] Recognizing and dealing with contamination [Re: WillieFunGuy] 1
#26622501 - 04/23/20 02:18 PM (3 years, 9 months ago) |
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Give her a shake š
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nevereataamanita


Registered: 09/08/19
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Re: [STICKY] Recognizing and dealing with contamination [Re: Crackatoa]
#26623669 - 04/23/20 11:47 PM (3 years, 9 months ago) |
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Is this some stage of knots/primordia I've never noticed before or am I growing something freaky?
-------------------- My LAGM 2020 Journal
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murderlabz
RIP Stoneman


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Posts: 551
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Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita]
#26623681 - 04/23/20 11:56 PM (3 years, 9 months ago) |
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Quote:
nevereataamanita said: Is this some stage of knots/primordia I've never noticed before or am I growing something freaky?

Looks like it's building up Metabolites getting ready for war with something.
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nevereataamanita


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Re: [STICKY] Recognizing and dealing with contamination [Re: murderlabz]
#26623689 - 04/24/20 12:03 AM (3 years, 9 months ago) |
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Damn, guess I'll keep an eye out and be ready to toss the shoebox. Here's a pic of the whole surface, I haven't spotted the contam yet.
-------------------- My LAGM 2020 Journal
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poisoned
untitled



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Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita]
#26624749 - 04/24/20 01:15 PM (3 years, 9 months ago) |
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Probably a bacteria, you won't really see it and you'll still get fruits.
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nevereataamanita


Registered: 09/08/19
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Re: [STICKY] Recognizing and dealing with contamination [Re: poisoned]
#26624761 - 04/24/20 01:19 PM (3 years, 9 months ago) |
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I've been wondering, is it a bad idea to clone or print from a bacterial grow? Is that shit going to get passed on or could you get away from it on agar?
-------------------- My LAGM 2020 Journal
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poisoned
untitled



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Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita] 1
#26624801 - 04/24/20 01:39 PM (3 years, 9 months ago) |
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usually fruits won't host other organisms on the inside.
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