Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Boomr Bag   Mushroom-Hut Liquid Cultures   Kraken Kratom Kratom Capsules for Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Capsules   Bridgetown Botanicals Bridgetown Botanicals

Jump to first unread post Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 20 hours, 8 minutes
Trusted Cultivator
Recognizing and dealing with contamination * 112
    #23130868 - 04/18/16 02:01 PM (7 years, 9 months ago)

This guide is a work in progress. Feel free to add any contributions, including additional information, high-quality pictures, and corrections to any inaccuracies. While there are many mushroom growing guides available, this one is unique in its focus on Cubensis. However, much of the information presented here can be applied to other mushroom species as well.

Signs of Contamination


Sectoring - This refers to the formation of distinct borders between two different fungal species growing in the same substrate. This phenomenon may be accompanied by the appearance of a line of metabolites where the two mycelia meet. In PF jars hourglass shaped mold colonies are typically caused by a compromised dry vermiculite layer, whereas circular mold colonies starting from an inoculation point are usually the result of contaminated inoculatant.



When the contaminant is bacterial the contaminated area appears uncolonized and are often rectangular shaped. When the mycelium encounters the infected area the edge loses it's thread-like appearance and metabolites may be present.



Sporophores - Sporophores are the structures of a fungus responsible for producing spores. In some cases, they can be visible to the naked eye without magnification. As the spores mature, most of them change color, making their presence obvious. However, with keen observation, they can be identified before reaching maturity. The early appearance of larger sporophores often resembles a small whisker with a white dot on the end.



Odor - Odor can be highly useful in identifying contaminants when they're not visible. It can also assist in distinguishing between mushroom mycelium and molds that resemble it. In some cases, a hidden mold in a spawn jar can be detected simply by smelling the spawn before use.

Odor can also play a helpful role in agar work. Cubensis cultures are known to have a distinct mushroomy odor. This aroma serves as a characteristic identifier during the process, providing a sensory clue to the nature of the culture.

Slime - A common indication of bacterial and yeast contamination is the development of a slimy appearance in the mycelium or grains. In areas where the substrate presses against the glass and condensation occurs, you may observe the presence of brown-yellow slimy rings around the grains. It's worth noting that oils and starches from burst grains can have a similar appearance, but they will already be present at the end of the sterilization process. Contaminants, on the other hand, will manifest later on, indicating an issue.



Dusty texture - As mentioned before, sporophores are usually too small to be seen individually. However, they often form a collective powdery layer that covers the substrate or mycelium. Paying attention to this texture is crucial for distinguishing sporophores from bruising or other discolorations that can occur in mushroom mycelium.



Discolorations - These are the most obvious signs of mold contamination. Molds often change color as they produce spores and some produce metabolites that can alter the color of the substrate.



There are also discolorations not caused by contamination, see "Not Signs of Contamination" below.

Overlay - Bright white mycelium that colonizes over a colonized substrate, casing layer or the vermiculite fruiting PF cakes have been rolled in is often the first sign of Trichoderma. Watch for the formation of green spores if these appear.



Soft patches - Contaminated substrate may become squishy or soft and crumble apart easily. The bright white patches Trichoderma can produce has the consistency of a squishy skin. PF cakes may crumble apart easily when contaminated.

Pinning in partially colonized spawn or PF jars - In a properly prepared PF or spawn jar this is contamination related. Unfortunately since the short half-pints have become difficult to find the tall jars are often used in the PF tek in which case that alone can cause pinning before full colonization. It's even common when pints are used for it so consider that first if no other signs are present.

Spongy mycelium - Molds, especially pins molds, can create a very dense mycelium in jars that seems to press against the glass and fill up the empty volume of the jar. The mycelium has a smooth texture compared to mushroom mycelium.




Not Signs of Contamination



Bruising - Bruising occurs when cell walls in mycelia/mushroom tissue are damaged. Most often this is result of touching, particularly while harvesting the mushroom, and can also occur from dehydration. Dehydration bruising is often widespread in the mycelium and occurs mostly around the base of pins and mushroom on that substrate. Bruising may be green or blue and very heavy bruising may appear black. Extreme bruising is normally found on the stumps of harvested mushrooms.



Spores - Spores produced by Cubensis are a dark violet with the exception of certain mutant/novelty strains. Spores often first appear on the torn veil but if left to sporulate mushrooms will drop large amounts of spores on the substrate and other mushrooms. Air currents may carry spores and deposit them on the tops of the caps.



"Mutant" Blobs - Blobs usually appear when pinning first begin with less blobs being produced later. Mutant blobs are the result of genetic abnormalities and are particularly common in degenerated cultures and certain varieties such as Penis Envy. Environmental conditions also seem to play a role in blob development. On PF cakes they are mostly seen in when cakes are fruited immediately after full colonization.



Rhizomorphs - Rhizomorphs are large threads of mycelium that carry nutrients to pins hence they occur when pinning has begun. Very large rhizomorphs may occur when nutrients are being transferred to a pin growing on a non-nutritious surface such as a glass jar or the sides of a plastic tub. Aerial rhizomorphs are usually seen when a casing layer is used. They appear as cottony strands poking out of the casing layer many of which eventually knot and grow into pins.



Metabolites - Although a large amount of metabolites often indicates contamination, small amounts are common on fully colonized substrates and spawn/PF jars. Metabolites are normally yellow but red metabolites sometimes occur in spawn jars. Metabolites may also appear as a yelllow discoloration of the mycelium.



Aborts - Pins that stop growing, develop a black cap, but are not slimy or show other signs of disease do not seem to be caused by pathogens.



In vitro pinning - Tan growths pressing against the glass, usually developing a dark circle in the center and eventually forming into obvious pins.





Identifying Contaminants



Penicillium

Penicillium is a common mold found in indoor air and is one of the most commonly encountered contaminants in agar and spawn jars. It produces tiny spores that stay airborne for relatively long periods of time. Early growth is white and can be difficult to distinguish from mushroom mycelium. Colonies are typically circular with a white edge. Most species sporulate very soon after first appearance, producing green, yellow, or blue-green spores. This mold contaminant has the ability to spread quickly, overwhelming any mushroom mycelium that may be growing in the same substrate. Contamination after spawning is uncommon and usually has little effect on yield. Penicillium is sourced from soil, food, compost, and air, and has musty or dirt-like odors.



Aspergillus

Aspergillus, like Penicillium, is a commonly found mold in indoor air. It produces tiny spores that have the ability to travel significant distances before settling. Many species of Aspergillus are known contaminants of mushroom substrates, posing a challenge to mushroom cultivation.

The mycelium of Aspergillus is typically light grey, exhibiting linear threadlike growth that can be mistaken for mushroom mycelium. Some colonies may appear ringlike, with denser mycelium concentration near the edges. Aspergillus can exhibit different appearances, ranging from sporophores similar to pin molds to a resemblance to Penicillium. The color and size of sporophores vary depending on the species and substrate, with yellow, black, green, blue, and grey being common. This variability can make it difficult to differentiate Aspergillus from other mold species.

Aspergillus finds its sources in soil, wood, dust, and the surrounding air. Its odor profile tends to be musty or dirt-like.



Trichoderma

Trichoderma, despite being less commonly found as free spores in indoor air compared to other molds, has earned a notorious reputation as one of the most prevalent contaminants in mushroom cultivation due to its aggressive nature. Trichoderma often finds its way indoors through dust particles, which act as common sources and primary carriers of this mold.

The mycelium of Trichoderma is usually transparent to light grey, making it quite challenging to spot, depending on the substrate. However, one of the first visible signs of Trichoderma contamination is the development of a thick, bright white aerial mycelium that grows over the surface of the substrate. On this mycelium, spores are produced, giving it a yellow to green color, often surrounded by a noticeable bright white "apron."

While the wet spores produced by Trichoderma are too heavy to become airborne on their own within a fresh colony, they can adhere to airborne dust particles and spread through that means. Trichoderma sporulation can be triggered by factors such as light, changes in nutrient availability in the substrate, full colonization, and damage to colonies.

Contamination of substrates by Trichoderma typically occurs after spawning. Freshly spawned grains are particularly vulnerable when exposed to a high concentration of Trichoderma spores. Cross-contamination can occur during handling, especially when coming into contact with sources of the spores, such as dust and soil. Although Trichoderma spores are easily killed by pasteurization, sterilizing certain bulk substrates can ironically make them more susceptible to contamination. However, the recovery of spawn grains plays a crucial role as well. Slow recovery, often due to the presence of bacteria in the grains, is a common cause of Trichoderma infection. Anything that slows down the recovery process is a significant risk factor.

Infected substrates may display uncolonized patches where the mold's mycelium has taken hold, indicating the presence of Trichoderma mycelium.

Visibly growing Trichoderma on living mushrooms is a rare occurrence. In cases where the mold infects a mushroom, obvious dark brown damage or heavy bruising will be noticeable. As a general rule, when harvesting from an infected substrate, if the mushroom appears healthy, it can be kept.




Mucor

Mucor, also known as pin mold, derives its name from the small, grey to black sporophores that resemble tiny pinheads. It bears a striking resemblance to Rhizopus, another common mold. When observed on bulk substrates, Mucor contamination is typically a consequence of contaminated spawn.

Mucor originates from soil, plants, and the surrounding air. Keeping a vigilant eye and implementing proper hygiene measures can help prevent the introduction and spread of this mold.

Rhizopus

Rhizopus, known for its rapid growth, is one of the swiftest invaders among common contaminants. Its appearance bears a strong resemblance to that of Mucor, making it easy to confuse the two. Rhizopus has the additional ability to parasitize damaged or aborted mushrooms, further complicating matters for mushroom cultivators.

One notable characteristic of Rhizopus is its tendency to produce substantial amounts of aerial mycelium. This mycelium, often visible as a cottony or fluffy growth, can rapidly spread across the surface of bulk substrates.

Sources of Rhizopus include soil and dust.



Fusarium

Fusarium is a mold that originates from various sources such as soil, plants, unsterilized grain, and humidifiers. It exhibits a white mycelium that closely resembles mushroom mycelium and has the potential to produce vibrant colors such as purple, pink, orange, and yellow. However, it's important to note that color changes may not be evident in short-lived agar cultures.

Fusarium contamination has most commonly been seen as a result of contaminated spore syringes. The mold looks like mushroom mycelium and grows at a similar speed. It can take two weeks before the colors develops.




Cladosporium

Cladosporium, a frequent contaminant mold in spawn, exhibits distinctive characteristics that set it apart. One of its most noticeable features is the presence of dark-green spores, which tend to change in color as they age, transitioning from green to grey or black.

This mold carries a musty odor, providing a helpful clue for its identification. It is commonly encountered in spawn during mushroom cultivation processes.


Alternaria

Alternaria, a common mold found on agar and spawn, is characterized by its typically black or dark grey appearance. It emits a musty odor, which can aid in its identification.


Chaetomium

Chaetomium is a green mold that can be identified by the presence of numerous small green to tan bur-like structures scattered across the substrate during the spawn run. In some instances, mushroom mycelium may colonize these areas over time.

One notable characteristic of Chaetomium is the high heat resistance of its spores compared to other fungi. This means that they can withstand relatively short pasteurization times or low pasteurization temperatures. As a result, Chaetomium contamination is more commonly encountered in straw or compost that has undergone insufficient pasteurization.


Monilia

Monilia is a mold that typically appears as a powdery growth in shades of white, grey, or pink. It can be encountered in various environments, including mushroom cultivation settings.

One particular form of Monilia, known as Neurospora, exhibits rapid growth with the development of an aerial mycelium. Over time, this mycelium undergoes a color change, turning bright red or orange, providing a distinct visual cue for identification.


Neurospora on casing layer


Scopulariopsis

Scopulariopsis is a mold that manifests as patches of white mold with a powdery appearance. Over time, the color of the mold may transition to a slight pinkish hue, especially after approximately a week.

This type of mold tends to thrive in substrates with high pH levels or high ammonia content. When Scopulariopsis contamination occurs, it can have a significant negative impact on mushroom cultivation. In severe cases, it can lead to a complete absence of mushroom development, greatly reducing the overall yield.

Coprinus

Coprinus is a common mushroom-producing contaminant frequently encountered in mushroom cultivation. Its presence is an indicator of excess ammonia in the substrate, particularly in manure-based substrates. This high ammonia content inhibits the growth of normal mushroom mycelium, leading to the proliferation of Coprinus.

These contaminants typically appear during the spawn run phase and continue to emerge until the ammonia levels are depleted. Once the substrate becomes more suitable for normal mushroom mycelium, it may eventually colonize the substrate and replace the Coprinus growth. There are various species of Coprinus, resulting in variations in their appearance. However, they are generally recognizable and distinguishable from Cubensis.





Schizophyllum

Schizophyllum commune is a mushroom-producing contaminant. It can thrive in various substrates and environments, and its growth can adversely affect the desired mushroom species being cultivated. Its presence indoors is typically attributed to contaminated inoculant or spawn used in the cultivation process.




Bacillus

Bacillus, with Bacillus subtilis being the most common species, is a bacterial contaminant frequently encountered in spawn. It is characterized by its distinctive odors, often described as resembling feet or rotten apples. When Bacillus contaminates the spawn, it manifests as a foul smell and the presence of brownish slime or crust on the grains.

The appearance of Bacillus bacteria is a sign that the sterilization process may have been insufficient. Additionally, Bacillus can be introduced through contaminated cultures or spores.

To prevent Bacillus contamination, it is crucial to ensure proper sterilization protocols are followed and maintained. Adequate drying of the spawn grains help minimize the risk of Bacillus growth.




Yeasts

Yeasts are a diverse group of microorganisms that can be encountered in mushroom cultivation, both in spawn and on agar. Yeast colonies actually resemble bacteria in their visual characteristics. Some yeasts are easily recognizable by the presence of tiny spots, typically white, pink, or yellow, that appear throughout the grain jar. However, there are other yeasts that are indistinguishable from bacteria based on visual appearance alone.

Yeast contamination is prevented with good sterile technique.




Pseudomonas

Pseudomonas is a genus of bacteria that contains many species, some of which can be found in mushroom cultivation. While certain strains of Pseudomonas are harmless or even beneficial to fruit bodies, problems can arise when there is prolonged contact between water and the mushroom surface. This often occurs due to condensation, misting, or excessive soaking.

When present in large amounts, Pseudomonas bacteria can produce enzymes that degrade the cell walls of the mushrooms. The symptoms of Pseudomonas contamination are typically superficial, manifesting as minor brown spotting on the mushroom surface. These spots generally do not affect the taste, texture, or odor of the mushroom. However, if the wet conditions persist, the contamination can progress to larger brown slimy patches and grooves, and may even lead to degradation of significant portions of the mushroom cap.

Since Pseudomonas bacteria are naturally present, prevention relies on providing adequate air exchange to facilitate the quick evaporation of moisture droplets. It is important to remove infected pins before misting, as the splashing can spread large amounts of the bacteria and accelerate the contamination process on other fruit bodies.




Cladobotryum

Cladobotryum is a genus of parasitic fungus that commonly infects mushrooms and casing layers in mushroom cultivation. It is typically sourced from soil and plants. There are multiple species of Cladobotryum, and the symptoms can vary depending on the specific species of mold and the type of mushroom being grown.

Cladobotryum infection leads to a condition known as cobweb disease. It is characterized by the growth of cobweb-like mycelium that covers the surface of the mushroom, resulting in the decay of the fruit body and heavy spore production. Spore spotting is particularly common when spores are present in the air. The growth and infection of Cladobotryum on the casing layer of mushrooms typically occur in later flushes, rather than during the initial pinning stage.

It is important to understand that Cladobotryum is primarily a casing layer and fruit body contaminant. It does not hinder the colonization of spawn or bulk substrate, nor does it serve as a source of infection for them. However, once the mushrooms have formed and the casing layer is in place, Cladobotryum can proliferate and cause significant damage to the fruit bodies.

The disease quickly spreads the substrate will stop producing healthy mushrooms and need to be discarded. You should avoid disturbing the contaminated substrate while indoors to prevent spores from being released into the indoor air.




Verticillium

Verticillium is a parasitic mold that can infect various mushroom species. It has multiple sources, including soil, plants, and flies. The severity and manifestation of Verticillium contamination can vary significantly.

The most common symptom of Verticillium infection is the appearance of brown spotting on the caps and stems of mushrooms. Unlike bacterial blotch, the spotting caused by Verticillium is dry, typically indented, and may develop a light gray fuzz in the center under high humidity conditions. The fungus often infects a specific side of a mushroom, causing growth to cease in that area. As healthy tissue continues to grow, it can lead to stem peeling, lesions, and cracking in the cap, which may result in a tilted appearance. When primordia (early stages of mushroom development) become infected, small "bubbles" of infected mushroom tissue may form. The infected tissue on both the bubbles and the growing mushrooms appears brown to gray and has a dry, leathery appearance.

Verticillium infection occurs when developing fruit bodies are exposed to Verticillium spores. Symptoms typically appear 7-14 days after exposure. Spawn is not typically a vector of contamination. Proper pasteurization of the substrate or casing material can destroy Verticillium spores, but maintaining good hygiene practices is essential to prevent recontamination.

The primary source of Verticillium spores is soil. Exposure can occur through airborne dust, insects, contact with contaminated surfaces (e.g., hands and clothes), and most notably, through aerosols created during misting. Even a single spray of water hitting a contaminated mushroom or surface can launch Verticillium spores up to two feet away.

Despite it's reputation, Verticillium infection is not common with indoor Cubensis and symptoms caused by bacteria are often mistaken for it.




Mycogone

Mycogone is a parasitic fungus that infects primordia (early stages of mushroom development) and small pins, resulting in monstrous deformations and rotten smelling fruitbodies. Like Verticillium this one has a big reputation but there's little evidence of it infecting Cubensis. Nevertheless strange growths of unknown causes are often blamed on it.



Cause of Contamination



Contaminated inoculant
Causes: Mold or bacteria in PF jars
        Mold or bacteria in grain spawn
        Mold or bacteria during or after spawn run (uncommon)

When a spore syringe is contaminated most or all jars inoculated with that syringe usually show the same contaminants. They are common problem for new cultivators. Obtain syringes from a reputable vendor and use peer reviewed techniques for making your own.

When spores transferred from a particular area of a spore print are contaminated the result is usually a single contaminated jar or plate. Any syringes made from that transfer will usually be contaminated. Limit transfers to small amounts of spores only to help avoid this. Avoid taking spores from outer edges of the printed area.

Liquid cultures are easily contaminated as all contaminants in the inoculant used to create them will be directly exposed to the medium (the broth). Contaminated liquid culture jars usually appear normal even when contaminated although heavy bacterial contamination can give it a cloudy appearance or create blob-like formations.

Agar cultures can be contaminated without showing signs. The contaminant could be something that landed on a colonized area or something growing beneath the mycelium. To avoid this isolate mycelium from spore or tissue inoculated plates by transferring a small piece of mycelium from the outer edge of the colony with an inoculation loop. Fully colonized plates should be avoided as the hyphae can grow up and out the sides of the plate. It may be helpful to smell the inoculation after a transfer if you suspect a contaminant may be present.

Botched inoculation
Causes: Mold or bacteria in grain spawn
        Mold or bacteria in PF jars
        Mold during or after spawn run
        Bacteria or stalling during spawn run

When steps have been taken to prevent cross-contamination this will result in a single contaminated jar, and at some or all inoculation points in PF jars. Things that can contaminate an inoculation include: touching a needle, scalpel, or inoculation with something that isn't not sterile, touching the sterile inside part of a jar with your gloves, exposure of sterile items to airborne contaminants, moving unsterile item over sterile areas, or moving unsterile items between the filter and sterile while using a flow hood.

Botched grain to grain transfer
Causes: Mold in spawn jars     
        Slow recolonization or stalling in spawn jars
        Slow colonization during spawn run
        Bacteria or stalling during spawn run
        Mold during or after spawn run (most common)
       
Similar causes to botched inoculations. The contaminant typically does not show up until the jar has been spawned. Contaminated g2gs often lead to a large number of jars becoming contaminated. G2Gs should always be done in a still air box or in front of a flow hood. Jars should be checked for odors before spawning. It's particularly important that jars and lids be disinfected as the vibrations created during the transfer can easily shake loose contaminants into the receiving jars.

Insufficient sterilization
Causes: Bacteria and stalling in spawn jars (most common)
        Bacteria and stalling in PF jars     

When sterilization temperature/times are not adequate bacterial contamination may occur. The bacteria is usually widespread in the substrate appearing days to weeks after the sterilization. It's important that steam fills the entire pressure cooker/pot before you start timing the procedure.

Larger substrates require longer times for the heat penetrate to the center, i.e. a gallon jar requires longer than 4 quart jars even though it's same amount of substrate.

Whole grain jars contain a lot of empty space between the grains which slow down heat penetration and require either a pressure cooker or very long sterilization times.

Compromised dry vermiculite layer
Causes: Mold or bacteria in PF jars (most common)
        Mold on dry vermiculite layer of PF jars
        Mold on PF cakes after birthing

When doing the PF the dry vermiculite layer on top acts as a filter. If it fails before the jar is fully colonized contaminants may start to grow in the uncolonized areas. This a common cause of contamination that first appears away from the inoculation points.

If the layer is shifted while moving the jar it creates an opportunity for the contaminated vermiculite on the surface to reach the sterile substrate. For that reason it's a good idea to have the layer fill the entire of the jar so the lid will help hold it in place. Coarse vermiculite in less effective so fine vermiculite should be used. If the layer becomes damp it will not prevent contaminants from reach the substrate.

Filter failure
Causes: Mold in spawn jars
        Bacteria or stalling in spawn jars       

When a filter becomes damaged, wet, or leaves any kind of open gap around the air holes

Improper pasteurization
Causes: Mold in later flushes (most common)
        Mold during spawn run or early flushes

Pasteurization temperatures range from 130-170F. The lower end of that range is not always sufficient in preventing survival of heat resistant molds.

In contrast, when temperatures are too high or sustained for too long it destroys more of the beneficial bacteria which would normally survive. Manure and compost substrates are most vulnerable to this. A common result from excessive pasteurization temperatures is incomplete colonization of the bulk substrate (early pinning) followed by visible Trichoderma infection.

Cross contamination after pasteurization
Causes: Mold during or after spawn run

When spawning it's important to limit exposure of the spawn grains and pasteurized substrate to contaminants. Hands, clothes, and hair are major vector for recontamination of pasteurized substrate. Cooling substrate should be protected from dust as much as possible.

Casing material is also vulnerable and contaminated casing material may lead to diseases like cobweb or verticillium. If using unpasteurized casing material it should be used straight from freshly opened or sealed bags.

Insufficient gas exchange during spawn run
Causes: Bacteria or stalling during spawn run
        Mold during spawn run
        Fermentation odor during spawn run

A lack a gas exchange while bulk substrates are colonizing creates anaerobic conditions which can stall the recovery and colonization of the mushroom mycelium and promote the growth of anaerobic bacteria. A fermentation odor may develop. In serious cases the mushroom mycelium does not recover allowing mold to colonize the grains.

Insufficient cooling of bulk substrate
Causes: Bacteria or stalling during spawn run
        Mold during spawn run

Mushroom mycelium is easily killed by high temperatures. All of the bulk substrate must be cooled to room temperature before spawning.

- Unfinished

*title edited 3/28/2020


Edited by fahtster (01/19/24 09:41 AM)


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Kizzle] * 7
    #23130900 - 04/18/16 02:16 PM (7 years, 9 months ago)

Fantastic work Kizzle, stickied :congrats:

I would like to chime in that verticillium fungicola has been reclassified as Lencacillium fungicola to distinguish it from the verticillium species that attack plants.

Very nice work man.


Extras: Filter Print Post Top
InvisibleGrey
ā‡œ ✯ ā‡
 User Gallery


Registered: 11/06/14
Posts: 6,223
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 3
    #23131962 - 04/18/16 06:50 PM (7 years, 9 months ago)

:takingnotes:

Excellent write up Kizzle.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


Extras: Filter Print Post Top
OfflinemyceliumEX
Philomathmycology
I'm a teapot User Gallery


Registered: 12/10/15
Posts: 153
Loc: South East US
Last seen: 3 years, 11 months
Re: Recognizing and dealing with contamination [Re: Grey] * 4
    #23132191 - 04/18/16 07:37 PM (7 years, 9 months ago)

:whathesaid:
Very easy to follow, and great info on how to identify contams.


--------------------

B.C. by Johnny Hart
"All mushrooms are edible, but some only once."
-- Croatian Proverb


Extras: Filter Print Post Top
Invisiblejimmyjams
Stranger

Registered: 04/18/16
Posts: 29
Loc: Earth
Re: Recognizing and dealing with contamination [Re: myceliumEX] * 2
    #23133079 - 04/19/16 12:54 AM (7 years, 9 months ago)

Great post! I've bookmarked it.:thumbup:


Extras: Filter Print Post Top
InvisibleSpicyWizard
I'm a teapot


Registered: 12/14/15
Posts: 511
Re: Recognizing and dealing with contamination [Re: jimmyjams] * 2
    #23143527 - 04/22/16 07:41 AM (7 years, 9 months ago)

great post! this is amazing, and ill deff be saving this as a bookmark!


Extras: Filter Print Post Top
OfflineGreenPrayingMantis
Stranger
 User Gallery
Registered: 03/20/16
Posts: 18
Last seen: 6 years, 5 months
Re: Recognizing and dealing with contamination [Re: SpicyWizard] * 2
    #23201691 - 05/08/16 09:38 AM (7 years, 9 months ago)

thanks for the very good guide


Extras: Filter Print Post Top
Offlinesauroman1
Emrys

Registered: 03/22/14
Posts: 204
Loc: Shangrila
Last seen: 13 hours, 44 minutes
Re: Recognizing and dealing with contamination [Re: GreenPrayingMantis] * 2
    #23225531 - 05/14/16 10:49 AM (7 years, 8 months ago)

How to deal with bacteria endospores? I have suspicion that spore prints might be contaminated after repeatedly getting ruined PF cakes.


--------------------
"You come from realms of unimaginable power and light, and you will return to those realms.ā€ ― Terence McKenna


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: sauroman1] * 2
    #23225653 - 05/14/16 11:23 AM (7 years, 8 months ago)

Usually endospores are a problem in cereal grain and we deal with them via pressure cooking. If your inoculate is the vector then you either need to get new prints or start working with agar to clean it up. Actually agar is the better road IMO. At that point tho most people get pressure cookers and start with grains.


Extras: Filter Print Post Top
Offlinesauroman1
Emrys

Registered: 03/22/14
Posts: 204
Loc: Shangrila
Last seen: 13 hours, 44 minutes
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 2
    #23230050 - 05/15/16 01:33 PM (7 years, 8 months ago)

Yes, agar dish has advantages, but used it only for cyanescens. Endospores can be problem in any nutritious enviroment. Question is what it's vulnerable sides compared to cube spores? Keeping in water for long time can eliminate spores?
Don't have currently access to pressure cooker. But I've read that for Pf cakes it's not prerequisite.


--------------------
"You come from realms of unimaginable power and light, and you will return to those realms.ā€ ― Terence McKenna


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 20 hours, 8 minutes
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 2
    #23274034 - 05/26/16 01:56 PM (7 years, 8 months ago)

How is the bacteria manifesting? I mean is it showing up right away? Is it localized to the inoculation points or what?


--------------------


Extras: Filter Print Post Top
OfflinePsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
Re: Recognizing and dealing with contamination [Re: Kizzle] * 2
    #23281741 - 05/28/16 03:47 PM (7 years, 8 months ago)

:thumbup:


--------------------
PsilocyBen's Trade List!


Extras: Filter Print Post Top
OfflineSgt. Overkill
Agar Warrior


Registered: 05/24/16
Posts: 10
Last seen: 6 years, 5 months
Re: Recognizing and dealing with contamination [Re: PsilocyBen17] * 2
    #23355549 - 06/17/16 05:52 PM (7 years, 7 months ago)

Great guide! Very informative


--------------------
Sgt. Overkill of the 23rd Agar Division, Reporting for Duty!


Extras: Filter Print Post Top
Invisiblefunkymonk22
In Service to the Ineffable..
Male User Gallery

Registered: 01/25/16
Posts: 469
Loc: The Big O
Re: Recognizing and dealing with contamination [Re: Sgt. Overkill] * 2
    #23357709 - 06/18/16 11:14 AM (7 years, 7 months ago)

Yes, great post here...kinda answered the wuestion in my post, but hoping for a more specific answer to my problem...great job


--------------------


"The clouds didn't look like cotton, they didn't even look like clouds.."-Townes Van Zandt


Extras: Filter Print Post Top
Offlinefundyfresh
Just bubbles
 User Gallery


Registered: 02/28/13
Posts: 11
Last seen: 7 years, 7 months
Re: Recognizing and dealing with contamination [Re: funkymonk22] * 2
    #23374176 - 06/23/16 03:34 PM (7 years, 7 months ago)

Any thoughts on this. After first flushhttp:/


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: fundyfresh] * 1
    #23374252 - 06/23/16 03:56 PM (7 years, 7 months ago)

Trich. At least ya got a flush.


Extras: Filter Print Post Top
OfflineSkillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 4 months
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 2
    #23391770 - 06/28/16 05:46 PM (7 years, 7 months ago)

Great post.  This is awesome.        Hey I have a question.  Sorry for the long text.  Wanted to explain quickly.    I was looking for a good place to check and I guess this is as good as any.      Ok so here's what happened.  Two of my cakes were too moist and started to ferment just a little.  I make wine and know the smell well and caught it early.    Mushrooms grow naturally around here and used to outback the neighbor said but someone dug a bunch up and never came back.    So I knew a perfect spot and dumped the cakes out in some shade but where light is perfect.  And hunidty and moisture is great.  I sprayed them down a bit so they would be rinsed and maybe grow hopefully.    I found a chunk of Mycellium growing about 2weeks later and it was under a leaf on a chunk of the cake I missed when rinsing.      I grabbed it up carefully and put it in some pasturized horse poo/straw/coco coir/Verm.  And put the chunk in the middle and layer of coco/Verm On top of it with some dry Verm too. 
I set it in an Aqaurium I had and slid it under my Motorhome so it wouldn't get rained on or in the sun.    Today here's what it looks like.    Is this anything I should keep still?    It really looked like the proper nice white Mycelium but now since its come thru the casing layer it's looking a tad bit different and just wanted a few opinions on wether j should keep or toss


Extras: Filter Print Post Top
OfflineDubtubs420
Stranger
 User Gallery
Registered: 07/21/16
Posts: 2
Last seen: 7 years, 6 months
Re: Recognizing and dealing with contamination [Re: Skillzd] * 2
    #23478319 - 07/26/16 10:39 AM (7 years, 6 months ago)

Is this contamination? Pitching it regardless, other jar has no progress, can post more photos.
Day 13 now for this jar.



Extras: Filter Print Post Top
Offlinenakano
Stranger
Registered: 05/21/16
Posts: 10
Last seen: 7 years, 4 months
Re: Recognizing and dealing with contamination [Re: Dubtubs420] * 2
    #23568780 - 08/23/16 10:47 AM (7 years, 5 months ago)

Basically anything not white, right?


Extras: Filter Print Post Top
OfflineShaker77
Stranger
 User Gallery

Registered: 04/30/16
Posts: 2
Last seen: 7 years, 4 months
Re: Recognizing and dealing with contamination [Re: nakano] * 2
    #23575511 - 08/25/16 09:49 AM (7 years, 5 months ago)

Good Morning, I was hoping that someone would be able to help me. I'm in my first flush and pining has started, however I have, what looks like some contamination occurring, on half of the surface,it looks like green mold, I tried to scoop it all out, but it just keeps coming back, should I salt that whole area, and hope for the best, or do I just let it go for now. Any help from someone with more knowledge then me would be greatly appreciated. Looking forward to hearing from someone. Thank-you


Extras: Filter Print Post Top
InvisibleGrey
ā‡œ ✯ ā‡
 User Gallery


Registered: 11/06/14
Posts: 6,223
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Shaker77] * 1
    #23577721 - 08/25/16 09:17 PM (7 years, 5 months ago)

If it's green,  it'll likely keep coming back. I would toss it or bury outside. 

If you're dead set on getting fruits,  and it's still growing them,  maybe don't keep it around your workspace/grows.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


Extras: Filter Print Post Top
OfflineSilentMajority420
Stranger

Registered: 09/02/16
Posts: 3
Last seen: 7 years, 3 months
Re: Recognizing and dealing with contamination [Re: Grey] * 1
    #23609729 - 09/03/16 11:02 PM (7 years, 5 months ago)

Hello individual people, and the One Cosmic Universe; same thing.

I have encountered the ghostly green gunk, and it was not pleasant. That smothering smug smell, which permeates it putrid spores over all that which is holy! The madness must cease, if I wish to find peace for me and my mushroom. So this brave knight woketh early when the cock crowed, and boomed "the cleansing will commence in this humble abode!"

So yea, woke up early, went to lowes, acquired equipment, and cleaned the ever living fuck out of my room. 10:1 H20/bleach sprayed the walls, containers, ceiling, everything. Moved 90% of stuff to basement, and kept the essentials in a single container. Cleaned the carpet, sprayed the carpet, ejaculated, but later cleaned up, on the carpet. Fucking hospitals ain't got shit on how sterile my room and closet is bruh. It better be since I spent 12 hours on that ass. No joke. Which makes me realize, now, several failed attempts later, why we need to listen to the experience 'old timers'. Growing is serious business. We will definitely get to play, but, we must first work diligently all the way through, before we blast off. We should treat it like we're building an actual rocket, to help us grow our metaphorical. Every nut and bolt is accounted for before ignition, and inspected multiple times. There are millions of dollars, and crew's lives at stake, so we must take every precaution. That is the way we should approach it; period.

I am going to vanquish thy enemy! No trich bitch is going to ruin my fun!


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: SilentMajority420] * 2
    #23609766 - 09/03/16 11:20 PM (7 years, 5 months ago)

You're kinda going about it wrong. If you're having issues with trich, cleaning your room isn't the answer. Working on clean spawn and clean agar inoculant is. You only need to be sterile when opening media. That means in your still air box or in front of a hood. Clean rooms have nothing to do with still air box work


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflineSilentMajority420
Stranger

Registered: 09/02/16
Posts: 3
Last seen: 7 years, 3 months
Re: Recognizing and dealing with contamination [Re: Mad Season] * 1
    #23611395 - 09/04/16 01:27 PM (7 years, 5 months ago)

Interesting.

I've been browsing this forum and cultivating for the past few months, and only now am I finding out about this magic 'agar'. It really helps to post and receive responses from experienced users, instead of just watching from the sidelines. Thanks for your help.


Extras: Filter Print Post Top
OfflineBhbuda
Stranger
 User Gallery
Registered: 10/17/16
Posts: 3
Last seen: 7 years, 3 months
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #23745426 - 10/17/16 12:16 PM (7 years, 3 months ago)

I'm new here and freaking out about what I have here. Sorry to restart an old post, but this seems the best place.
So in the pictures you can see the colour of the caps are weird, and there are green streaks in the stems. Basically what could cause this, and is it safe to eat?
Sorry again for being a forum noob.



Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Bhbuda] * 1
    #23745432 - 10/17/16 12:18 PM (7 years, 3 months ago)

Mostly bruising. Looks like there's some insane vert in there. Hella fuzzy feet from no fresh air too, which is what vert thrives in.. is this a kit done in a bag? Those things have the worst conditions for mushrooms. Make a proper fruiting chamber like sgfc.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflineBhbuda
Stranger
 User Gallery

Registered: 10/17/16
Posts: 3
Last seen: 7 years, 3 months
Re: Recognizing and dealing with contamination [Re: Mad Season] * 1
    #23745461 - 10/17/16 12:30 PM (7 years, 3 months ago)

Thanks for the fast reply. Yea, they a the tubs in the bags which you only have to flower (I'm lazy). Used them a few times before, but never looked like this, normally I get better fresh air so I don't get the white fuzz so much. This one just went very wrong. If these were yours, would you consume them?

Also, I'm 99% sure they were ment to have brown/golden tops. Is the white heads due to the same infection?


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Bhbuda] * 1
    #23745515 - 10/17/16 12:50 PM (7 years, 3 months ago)

Looks like it could just be leucism since they're still sporulating purple spores. There's a few strains which seem prone to this. Like mckenaii, and the obvious AA+

Vert is 100% edible, and almost never gets properly identified, yet nobody goes to hospital from bad medical shit. I'm just saying one of them are exhibiting the dots at the base of the stem, which is a verticillium indicator. I wouldn't print these guys either. Just take better care of them, and grow properly next time :wink: them suckers love air. More than humidity tbh.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflineBhbuda
Stranger
 User Gallery

Registered: 10/17/16
Posts: 3
Last seen: 7 years, 3 months
Re: Recognizing and dealing with contamination [Re: Mad Season] * 1
    #23745540 - 10/17/16 12:58 PM (7 years, 3 months ago)

So basically like an albino mushroom? Cool!

These ones are produced by "supa grow" and the type was "Brazil".
Thank you so much, helped me with my stress levels. Sorry once again if I didn't use this section properly.


Extras: Filter Print Post Top
OfflineDlurch314
Stranger
 User Gallery
Registered: 10/21/16
Posts: 21
Last seen: 3 years, 2 months
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #23841932 - 11/17/16 02:15 PM (7 years, 2 months ago)

Any idea what this is?



Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Dlurch314] * 1
    #23841986 - 11/17/16 02:29 PM (7 years, 2 months ago)

Trich


Extras: Filter Print Post Top
OfflineDlurch314
Stranger
 User Gallery
Registered: 10/21/16
Posts: 21
Last seen: 3 years, 2 months
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 1
    #23842059 - 11/17/16 02:51 PM (7 years, 2 months ago)

It's not green though. If it is trich is there anything I can do?


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Dlurch314] * 1
    #23842287 - 11/17/16 03:44 PM (7 years, 2 months ago)

Depending on a few factors trich before pins means the spawn was fucked and the whole tub will green up in no time. You can salt trich if the flush is almost done to hold it off for a day or two but this early in the game means it's probably all trash.

You can hang on to it for a day if you want to be sure, it will probably be green in a day and you will know for sure.


Extras: Filter Print Post Top
OfflineDlurch314
Stranger
 User Gallery
Registered: 10/21/16
Posts: 21
Last seen: 3 years, 2 months
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 1
    #23842585 - 11/17/16 05:02 PM (7 years, 2 months ago)

Yeah this one just started pinning a couple of days ago


Extras: Filter Print Post Top
OfflineChickie
Stranger
Registered: 11/20/16
Posts: 1
Last seen: 7 years, 20 days
Re: Recognizing and dealing with contamination [Re: Dlurch314] * 1
    #23850888 - 11/20/16 09:27 AM (7 years, 2 months ago)

Hello. This is my first grow. Started from a kit with 18 jars and GT spore syringes. Inoc on 11/12. Colonization observed on some jars 3 days in. Today, 8 days after inoc, all jars showing colonization with 2 jars with very minimal signs. All jars have what appear to be healthy mycelium. Notice one jar today with yellowing spots. Can anyone please suggest whether this is contaminate or water damage? My understanding is water damage appears yellow. Also, the area that is yellow appears wet...wetter then rest of jar, if you will. Please see pic. I have isolated the suspect jar from incubator for now. Thanks for your help.



Edited by Chickie (11/20/16 07:41 PM)


Extras: Filter Print Post Top
OfflineMistylee
Stranger
Registered: 11/20/16
Posts: 1
Last seen: 7 years, 2 months
Re: Recognizing and dealing with contamination [Re: Kizzle] * 2
    #23852515 - 11/20/16 07:23 PM (7 years, 2 months ago)

Moved over a fruiting jar (dunked it) a few days ago and it seems kinda fuzzy today.  I looked through all of the contaminant images and couldn't find any that look like this.  Just wanted to post this and get some thoughts.  The other jars were just moved in today.








Extras: Filter Print Post Top
Offlinepsychedethiogenic
Stranger
Registered: 07/11/16
Posts: 19
Last seen: 5 years, 3 months
Re: Recognizing and dealing with contamination [Re: Mistylee] * 1
    #23892152 - 12/03/16 09:29 PM (7 years, 2 months ago)

Can anyone please tell me if it is possible for me to save this jar, or should I just toss iht?






Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: psychedethiogenic] * 1
    #23892169 - 12/03/16 09:34 PM (7 years, 2 months ago)

That is completely fucked.


Extras: Filter Print Post Top
InvisibleSpicyWizard
I'm a teapot


Registered: 12/14/15
Posts: 511
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 1
    #23892769 - 12/04/16 02:53 AM (7 years, 2 months ago)

Quote:

Pastywhyte said:
That is completely fucked.



:punish2::whathesaid: :urreallydumb:  :kidreally:


Extras: Filter Print Post Top
Offlinepsychedethiogenic
Stranger
Registered: 07/11/16
Posts: 19
Last seen: 5 years, 3 months
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 1
    #23893393 - 12/04/16 10:39 AM (7 years, 2 months ago)

Do you happen to know what went wrong? I took extra sterilization caution and kept the jar at around 73 to 75 degrees although I think at one point the temp rose to at least 80 degrees if not more. I also noticed quite a bit of moisture.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: psychedethiogenic] * 1
    #23893565 - 12/04/16 11:43 AM (7 years, 2 months ago)

Hard to say. If it's in every jar it's probably a bad syringe or bad inoculation procedure.


Extras: Filter Print Post Top
Offlinekrudam2
Stranger
 User Gallery
Registered: 11/02/16
Posts: 20
Last seen: 7 years, 28 days
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 1
    #23939886 - 12/19/16 08:51 AM (7 years, 1 month ago)

thanks for the post.

could we get a picture of metabolites?

I don't know the difference between normal metabolites and infection, and I've had a commercial oyster bag (bought at store) have a large orange bit but customer service told me it was fine and it seemed to grow fine


Extras: Filter Print Post Top
OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 13 days, 3 hours
Re: Recognizing and dealing with contamination [Re: psychedethiogenic] * 1
    #23951013 - 12/23/16 08:02 AM (7 years, 1 month ago)

Well whats the deal with that lid?


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.ā€
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 20 hours, 8 minutes
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: krudam2] * 1
    #23984419 - 01/05/17 04:31 PM (7 years, 1 month ago)

Quote:

krudam2 said:
thanks for the post.

could we get a picture of metabolites?

I don't know the difference between normal metabolites and infection, and I've had a commercial oyster bag (bought at store) have a large orange bit but customer service told me it was fine and it seemed to grow fine



I added some. Metabolites normally appear in very amounts as in the pics but if the substrate has fully colonized for quite a while or it's been sitting in particularly warm conditions you might see more. If you're seeing large puddles or uncolonized areas then it could still be metabolites your seeing but probably the result of a contaminant.


--------------------


Extras: Filter Print Post Top
OfflineScottishwarrior
Grower
Male User Gallery


Registered: 05/13/16
Posts: 16
Loc: Indiana
Last seen: 6 years, 11 months
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #23987543 - 01/06/17 07:26 PM (7 years, 1 month ago)

Excellent article. I always send people the link to this thread when they ask questions about contamn.


Extras: Filter Print Post Top
OfflineJennifer1
Stranger
 User Gallery
Registered: 01/10/17
Posts: 3
Last seen: 7 years, 1 month
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #23998459 - 01/10/17 02:53 PM (7 years, 1 month ago)

Smells like coconut! Thank you


Extras: Filter Print Post Top
InvisibleMushenstein
Brain Damage
I'm a teapot User Gallery


Registered: 02/19/15
Posts: 680
Loc: At The Gates
Re: Recognizing and dealing with contamination [Re: Jennifer1] * 1
    #24063715 - 02/03/17 09:22 PM (7 years, 13 days ago)

Wow, That was an impressive read. Damn fine job.


--------------------
This is how I get down!


Muffler Tek
Slump's BRF Grinder Tek
My Agrocybe Aegerita Grow Log


Extras: Filter Print Post Top
OfflineAquatic Wombat
Homie
Male User Gallery
Registered: 03/24/17
Posts: 1
Loc: Cali
Last seen: 5 years, 9 months
Re: Recognizing and dealing with contamination [Re: Mushenstein] * 1
    #24198792 - 03/28/17 02:48 AM (6 years, 10 months ago)

I'm a total noob at this. This is my first attempt. I had 2 spore syringes and decided to inoculate 3 cakes and jar of liquid per syringe. I sterilized a small room, enclosed it with plastic sheeting, spritzed everything with a bleach solution, used a sterilized glove box, wore a tyvex suit, gloves, a respirator, flame sterilized the needle with an alcohol burner, sterilized the cakes in a pressure cooker @15 p.s.i. for 20 min. and the liquid for 15 to avoid caramalezation and after inoculation everything has been between 70-80 F. Mycelium growth in all six cakes appears fine after 4 days. One of the liquid cultures appears to have no activity while the other has this puffy ball with black spores from the syringe on it. Is this F.U.B.A.R.? Should I inoculate one new cake and see what happens, or should I just pitch it? Thanks.


Extras: Filter Print Post Top
OfflineAliceinwonderland3
Female User Gallery
Registered: 03/29/17
Posts: 6
Last seen: 6 years, 6 months
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #24203225 - 03/29/17 05:07 PM (6 years, 10 months ago)

I was also hoping to identify this "stuff" in my one jar... Not to hopeful about it :-(




Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Aliceinwonderland3] * 1
    #24203243 - 03/29/17 05:15 PM (6 years, 10 months ago)

Quote:

Aquatic Wombat said:




Defintely pitch, try to germinate and clean on agar before trying liquids.

Quote:

Aliceinwonderland3 said:
I was also hoping to identify this "stuff" in my one jar... Not to hopeful about it :-(






Idk it down to the species or even the genus, but it's definitely not clean. Looks like aspergillus maybe.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflineZachorion
 User Gallery

Registered: 03/06/17
Posts: 14
Last seen: 5 months, 3 days
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #24203642 - 03/29/17 07:48 PM (6 years, 10 months ago)

My first Pasty Plate agar try.  I'm guessing this is Penicillium?  It's the only one so far so I'm hoping the others are viable.


Extras: Filter Print Post Top
Offlinedart21
Stranger
Registered: 03/01/17
Posts: 14
Last seen: 6 years, 7 months
Re: Recognizing and dealing with contamination [Re: Zachorion] * 1
    #24225537 - 04/07/17 12:55 PM (6 years, 10 months ago)

:thumbup: Probs Zach, but cannot be certain from that photo.


Edited by dart21 (04/07/17 12:57 PM)


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,930
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: dart21] * 1
    #24233674 - 04/10/17 11:05 PM (6 years, 10 months ago)



1. Should I be concerned or worried about the cloudier, more solid area in the middle of the plate? I always notice my initial transfer wedge, being higher up than the level plate surface, bubbles up cloudy-like until it flattens out on the receiving plate's horizon. Is that what I'm seeing here?

2. Should the big divide between 11 and 12 concern me?


Extras: Filter Print Post Top
OfflineDlurch314
Stranger
 User Gallery

Registered: 10/21/16
Posts: 21
Last seen: 3 years, 2 months
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #24234306 - 04/11/17 09:06 AM (6 years, 10 months ago)

Hey guys in not sure if this is bruising or some kind of contam any ideas?



Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Dlurch314] * 1
    #24237185 - 04/12/17 10:42 AM (6 years, 10 months ago)

Bruising because its hella contaminated with bacteria


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflineDlurch314
Stranger
 User Gallery

Registered: 10/21/16
Posts: 21
Last seen: 3 years, 2 months
Re: Recognizing and dealing with contamination [Re: Mad Season] * 1
    #24237454 - 04/12/17 12:57 PM (6 years, 10 months ago)

So what would you do in my case? Would you pitch it or give it a shot?


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,930
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Dlurch314] * 1
    #24237630 - 04/12/17 02:05 PM (6 years, 10 months ago)

lol, absolutely get rid of it. Give what a shot? Releasing bacteria into your living area? That's not a shot; it's a guarantee.


Extras: Filter Print Post Top
OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 13 days, 3 hours
Re: Recognizing and dealing with contamination [Re: stareatclouds] * 1
    #24237645 - 04/12/17 02:11 PM (6 years, 10 months ago)

Yea i dont like the look of that, transfer away from the cloudiness


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.ā€
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


Extras: Filter Print Post Top
OfflineDlurch314
Stranger
 User Gallery

Registered: 10/21/16
Posts: 21
Last seen: 3 years, 2 months
Re: Recognizing and dealing with contamination [Re: stareatclouds] * 1
    #24241042 - 04/13/17 05:17 PM (6 years, 9 months ago)

Already in the dumpster thanks man


Extras: Filter Print Post Top
Invisibleoutofservice
I never get to touch no monkey
Male
Registered: 04/27/17
Posts: 160
Loc: Sun & Sand Flag
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #24470851 - 07/09/17 06:10 PM (6 years, 7 months ago)

Good info, thanks.

After reading this thread I thought these were contaminated, need a second opinion.

They are Pan. Cyan Cambo Sandose.  This is my first grow.
Noc on 5-17, I had two jars start colonizing almost a month after inoc. I've had the rest of the jars sitting in a dark closet waiting for the last two to get to 100%. The closet averages 77-80F, 40-46% humidity.
I've been told the yellow circle is a sclerotia. I hope it is, that would be a cool surprise.

I'm growing these because they're f*cking awesome! A friends friend grew them some time ago and I was hooked. So now I'm trying to grow them. I had my friend look at them and he told me he thought he remembered bluing when his friend grew them.

What are your opinions?  Good or toss?
Anything I can do to save them? Let them sit under a UVC light if needed?

If they're f*cked, is it because I let them sit too long?

I read this in another thread, "Dip them into a sol'n of %10 H2O2, drain them, and return them to their jars."  Is this an option?





--------------------


Edited by outofservice (07/10/17 07:56 PM)


Extras: Filter Print Post Top
Invisibleoutofservice
I never get to touch no monkey
Male
Registered: 04/27/17
Posts: 160
Loc: Sun & Sand Flag
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #24470861 - 07/09/17 06:15 PM (6 years, 7 months ago)

Is this Bacillus? If no, what is it?  5 grain, Mexis Jalisco. Trying for stones.



--------------------


Extras: Filter Print Post Top
Invisibleoutofservice
I never get to touch no monkey
Male
Registered: 04/27/17
Posts: 160
Loc: Sun & Sand Flag
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #24473617 - 07/10/17 07:34 PM (6 years, 7 months ago)

Hey All,

I plan to birth, dunk and roll tmw.

If I could some feedback on my post above with the pics (Pan Cyan Cambo), it would be greatly appreciated.  I don't want to risk contaminating the other jars I have (Mex jalisco and Tampanensis).


--------------------


Extras: Filter Print Post Top
Offlinestewboy350
Stranger
 User Gallery

Registered: 02/10/13
Posts: 31
Last seen: 4 years, 7 months
Re: Recognizing and dealing with contamination [Re: outofservice] * 1
    #24483771 - 07/14/17 08:51 PM (6 years, 7 months ago)

http://cobweb ?


Extras: Filter Print Post Top
Invisibleoutofservice
I never get to touch no monkey
Male
Registered: 04/27/17
Posts: 160
Loc: Sun & Sand Flag
Re: Recognizing and dealing with contamination [Re: Kizzle] * 2
    #24514029 - 07/28/17 01:25 PM (6 years, 6 months ago)

This


--------------------


Extras: Filter Print Post Top
OfflineSteevo
Just a cog in the death wheel
 User Gallery


Registered: 03/02/17
Posts: 2,702
Loc: Here and There
Last seen: 1 year, 9 months
Re: Recognizing and dealing with contamination [Re: outofservice] * 1
    #24514083 - 07/28/17 01:50 PM (6 years, 6 months ago)

Those grains look a little milky in that second pic. I haven't seen Jalisco colonizing before so it could be fine
Why are you planning on birthing, dunking and rolling that? You can just let it go in the bag for stones :shrug:


--------------------


Extras: Filter Print Post Top
Invisibleoutofservice
I never get to touch no monkey
Male
Registered: 04/27/17
Posts: 160
Loc: Sun & Sand Flag
Re: Recognizing and dealing with contamination [Re: Steevo] * 1
    #24514949 - 07/28/17 08:45 PM (6 years, 6 months ago)

Quote:

Steevo said:
Those grains look a little milky in that second pic. I haven't seen Jalisco colonizing before so it could be fine
Why are you planning on birthing, dunking and rolling that? You can just let it go in the bag for stones :shrug:




The bag is for stones. I had a guy who is familiar with the Mexicans say it does colonize in a funky way. Regardless, I'll let it be and ride it out, maybe I'll get some stones.
The jars are what I dunked and cased.


--------------------


Extras: Filter Print Post Top
Offlinemargaretlcarlson
Stranger
Registered: 08/20/17
Posts: 1
Last seen: 6 years, 3 months
Re: Stinky cheese smell. [Re: Kizzle] * 1
    #24568191 - 08/20/17 07:54 PM (6 years, 5 months ago)

First of all let me apologize, I am brand new to this site and to cultivating mushrooms. I am finding the site a challenge to navigate. I inoculated two agar plates one week ago and while as far as I can tell the growth on the plates looks normal, they smell strongly of stinky cheese, the mushy, aged kind. I don't know what, if anything, they are supposed to smell like.


Extras: Filter Print Post Top
OfflineCrispykoot
Jello Wrangler
Male User Gallery


Registered: 10/16/16
Posts: 5,922
Loc: Flag
Last seen: 1 day, 18 hours
Trusted Cultivator
Re: Stinky cheese smell. [Re: margaretlcarlson] * 1
    #24610007 - 09/06/17 09:01 PM (6 years, 5 months ago)

Quote:

margaretlcarlson said:
First of all let me apologize, I am brand new to this site and to cultivating mushrooms. I am finding the site a challenge to navigate. I inoculated two agar plates one week ago and while as far as I can tell the growth on the plates looks normal, they smell strongly of stinky cheese, the mushy, aged kind. I don't know what, if anything, they are supposed to smell like.




Welcome to Shroomery!

Always post pictures if you can...It helps people help you. Don't open your plates up to smell them. That will contaminate them.


--------------------






:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:


Extras: Filter Print Post Top
OfflineKrombopulosM
Mycoshaman
I'm a teapot User Gallery


Registered: 08/17/17
Posts: 674
Loc: Northeast Florida Flag
Last seen: 5 years, 3 months
Re: Recognizing and dealing with contamination [Re: Crispykoot] * 1
    #24652494 - 09/22/17 08:09 PM (6 years, 4 months ago)

Please help me identify if this aerial rhizo is caused by bacteria of some sort




Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: KrombopulosM] * 1
    #24652496 - 09/22/17 08:10 PM (6 years, 4 months ago)

Maybe a little stressed but nothing to fret over. Will probably be fine.


Extras: Filter Print Post Top
OfflineKrombopulosM
Mycoshaman
I'm a teapot User Gallery


Registered: 08/17/17
Posts: 674
Loc: Northeast Florida Flag
Last seen: 5 years, 3 months
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 1
    #24654159 - 09/23/17 04:59 PM (6 years, 4 months ago)

thanks, i just made some AA so im gonna transfer to those from my 3rd gen to hopefully get contam free myc for gen 4


Extras: Filter Print Post Top
OfflineKoka
Stranger
 User Gallery

Registered: 09/29/17
Posts: 1
Last seen: 6 years, 4 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] * 1
    #24670345 - 09/29/17 02:33 PM (6 years, 4 months ago)







[url=https://files.shroomery.org/files/17-39/671678266-20170928_151102.jpg][image]http://www.shroomery.org/forums/thumbs/17-39/671678266-thumb_20170928_151102.jpg[/image[url=http://files.shroomery.org/files/17-39/671677843-20170928_151050.jpg]
Hi guys can anyone tell me are these mushrooms bad or good ? Its my first time growing have no clue. thanks for help


Extras: Filter Print Post Top
Offlinepaegan
Stranger
Registered: 10/04/17
Posts: 35
Last seen: 5 years, 7 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Koka] * 1
    #24685602 - 10/05/17 10:41 AM (6 years, 4 months ago)



Contam or no contam?


--------------------
Virtual Reality, Analog


Extras: Filter Print Post Top
OfflineChaostoOrder
Lakota Peji Wicasa,
Male User Gallery


Registered: 08/01/09
Posts: 553
Last seen: 1 year, 2 months
Re: Recognizing and dealing with contamination [Re: paegan] * 1
    #24744651 - 10/29/17 02:03 AM (6 years, 3 months ago)

Beautiful Sticky thank you very much!! I believe one needs to study the contaminates just as much, if not more, than the actual species being attempted or identified.  This is very informational thank you!! good job!!!


--------------------
Too weird to live, to rare to die....-Hunter S. Thompson


:
[url=https://files.shroomery.org/files/10-21/47399604


Extras: Filter Print Post Top
InvisibleRadagastthebrown
Male


Registered: 10/22/17
Posts: 17
Loc: United states
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] * 1
    #24816299 - 11/30/17 08:31 AM (6 years, 2 months ago)

Now this here is very helpful and I think everyone needs to read this  a few times especially beginners or anyone who wants to truly understand mycology.
Bookmarked and read a few times, will be passing on the info.
Thank you for this.


Edited by Radagastthebrown (11/30/17 08:33 AM)


Extras: Filter Print Post Top
Offlinefukuzi
Stranger
Registered: 12/04/17
Posts: 6
Last seen: 6 years, 1 month
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #24829305 - 12/06/17 06:11 AM (6 years, 2 months ago)

Hello yellow, some pro outlook is required on these PFtek thingys. They've been around 4 weeks @ 23-28celcius, how do they look like and do they have a bright future? 1 of them shows not much signs of life, but what about these 2 others?

So they 2 are mostly white, but there is some yellowish colors and little bit black. This is a first try, so some pro comments would be really important.

Side A

Side B


Extras: Filter Print Post Top
Offlinemushroom_mode
Stranger
I'm a teapot User Gallery

Registered: 05/24/16
Posts: 74
Last seen: 3 years, 11 months
Re: Recognizing and dealing with contamination [Re: fukuzi] * 1
    #24862473 - 12/22/17 04:50 PM (6 years, 1 month ago)

can a contaminated (unmodified) tub infect a nearby healthy tub if both lids are ajar?

is it worth trying to fruit a tub with a light alcohol odour when the lid is opened?

using coir + oats


Extras: Filter Print Post Top
Offlinebeltain
Stranger
Registered: 12/20/17
Posts: 10
Last seen: 5 years, 10 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25095261 - 03/27/18 04:55 PM (5 years, 10 months ago)

so many ways for it to go wrong! I'm gonna try to grow soon and hope to encounter none of these guys. Thanks for the excellent write-up!


Extras: Filter Print Post Top
OfflineTheGreatGonzo
Stranger
 User Gallery
Registered: 04/14/18
Posts: 6
Last seen: 5 years, 7 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25140648 - 04/15/18 09:54 AM (5 years, 9 months ago)

Thanks for the info Kizzle, so much knowledge in one place!


Extras: Filter Print Post Top
OfflineJiM aYYYY
GreenGoblin
Male User Gallery

Registered: 03/06/18
Posts: 266
Last seen: 4 years, 1 month
Re: [STICKY] Recognizing and dealing with contamination [Re: TheGreatGonzo] * 1
    #25147891 - 04/18/18 11:36 AM (5 years, 9 months ago)


This is my first tray. I over misted and made some pools on accident overnight. Lesson learned.


Extras: Filter Print Post Top
OfflineGroggybandito
Stranger
 User Gallery
Registered: 09/12/17
Posts: 1
Last seen: 5 years, 9 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25170085 - 04/27/18 07:35 PM (5 years, 9 months ago)

Is this contamination, it is in a shotgun furting chamber, 99% R/H, fan 5-6 times a day, been in the chamber for 3 days, brown rice flower verm,


Extras: Filter Print Post Top
Offlineraoulduke948
Stranger
 User Gallery

Registered: 05/10/18
Posts: 6
Last seen: 5 years, 9 months
Re: Recognizing and dealing with contamination [Re: Groggybandito] * 1
    #25195105 - 05/10/18 09:13 AM (5 years, 9 months ago)

what do you think about those , they are contamined or just bruising ?


Extras: Filter Print Post Top
OfflineJiM aYYYY
GreenGoblin
Male User Gallery

Registered: 03/06/18
Posts: 266
Last seen: 4 years, 1 month
Re: Recognizing and dealing with contamination [Re: raoulduke948] * 1
    #25195129 - 05/10/18 09:32 AM (5 years, 9 months ago)

Quote:

raoulduke948 said:
what do you think about those , they are contamined or just bruising ?



Contamination for sure. Sorry for the loss man


Extras: Filter Print Post Top
Offlineraoulduke948
Stranger
 User Gallery

Registered: 05/10/18
Posts: 6
Last seen: 5 years, 9 months
Re: Recognizing and dealing with contamination [Re: JiM aYYYY] * 1
    #25195541 - 05/10/18 12:57 PM (5 years, 9 months ago)

I will restart one of them , and one i cleaned , i hope it's gonna be ok , i will come with news soon , cheers ! And thx for support !


Extras: Filter Print Post Top
OfflineSamAtticus
Male User Gallery
Registered: 02/27/18
Posts: 175
Last seen: 6 months, 16 days
Fantastic [Re: Kizzle] * 1
    #25198690 - 05/11/18 11:54 PM (5 years, 9 months ago)

Amazingly helpful (and unnerving) index, especially useful are the pics of various contaminants. This must have taken months to put together. Fantastic work!

I must admit, though, I'm a little confused by this paragraph:

"Overlay - A common sign of Trichoderma is mycelium that colonizes over a casing layer or the vermiculite fruiting PF cakes have been rolled in. While mycelium can also create overlay, if your fruiting conditions have been adequate to prevent it, this can be one of the first signs you notice."

I'd love some clarification if and when you have the time or inclination.

Again, beautiful work! Thank you!


--------------------

"I learn by going where I have to go." Theodore Roethke


Bod's AF Search Engine Tek


Extras: Filter Print Post Top
OfflineGonzo the Eternal
In Sterquiliniis Invenitur

Registered: 05/09/18
Posts: 480
Last seen: 4 years, 5 months
Re: Fantastic [Re: SamAtticus] * 1
    #25198817 - 05/12/18 02:17 AM (5 years, 9 months ago)

Great write up! And yes I agree with the guy up top, unnerving as well.


Extras: Filter Print Post Top
OfflineJiM aYYYY
GreenGoblin
Male User Gallery

Registered: 03/06/18
Posts: 266
Last seen: 4 years, 1 month
Re: Fantastic [Re: Gonzo the Eternal] * 1
    #25205351 - 05/15/18 11:24 AM (5 years, 8 months ago)


What type of contamination would you call this?


Extras: Filter Print Post Top
Invisiblenix21
Stranger
 User Gallery


Registered: 05/29/18
Posts: 622
Re: Recognizing and dealing with contamination [Re: JiM aYYYY] * 1
    #25235804 - 05/29/18 10:15 AM (5 years, 8 months ago)

I found some weird spot in my BRF cake, i don't know can you tell me whats going on with this pictures.. today is 19 day after inoculation (koh samui, ms syringe) and everything looks great, but this spot!.. i can tell for sure nothing is growing there (for now) it looks like a bit wet and maybe missing some brown rice flour, but I'm 80% sure that wasn't there few days ago.. also those in picture that looks like black dots in that area isn't black dots but holes in verm/brf mixture.. also i steam sterilized 9 jars for 2 hours... all doing great... and this is 277ml jar if that's important..






--------------------


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 20 hours, 8 minutes
Trusted Cultivator
Re: Fantastic [Re: SamAtticus] * 1
    #25267099 - 06/14/18 02:59 AM (5 years, 7 months ago)

Quote:

SamAtticus said:
Amazingly helpful (and unnerving) index, especially useful are the pics of various contaminants. This must have taken months to put together. Fantastic work!

I must admit, though, I'm a little confused by this paragraph:

"Overlay - A common sign of Trichoderma is mycelium that colonizes over a casing layer or the vermiculite fruiting PF cakes have been rolled in. While mycelium can also create overlay, if your fruiting conditions have been adequate to prevent it, this can be one of the first signs you notice."

I'd love some clarification if and when you have the time or inclination.

Again, beautiful work! Thank you!



Thanks for pointing that out. In part it's confusing because I meant to upload some better pictures later but I also rewrote it so it makes more sense. Basically what I meant is when you notice mycelium growing over parts of the substrate you normally don't see it growing particularly in comparison with identically made substrates. When you have an actual casing layer you normally aim for it to not be colonized, and to mostly have rhizomorphs growing through it but then suddenly there's this patch growing over it and...
:scream:


--------------------


Extras: Filter Print Post Top
Invisiblenube424
Male
Registered: 12/03/17
Posts: 6,063
Re: Fantastic [Re: JiM aYYYY] * 1
    #25295857 - 06/27/18 08:45 PM (5 years, 7 months ago)

Quote:

JiM aYYYY said:

What type of contamination would you call this?



Um I think it's contaminated with psilocybe cubensis?


Extras: Filter Print Post Top
Offlinetombonytom
Stranger
Registered: 07/06/18
Posts: 1
Last seen: 5 years, 3 months
Re: Recognizing and dealing with contamination [Re: nube424] * 1
    #25312278 - 07/06/18 07:18 PM (5 years, 7 months ago)

just made a liquid culture with honey, took my old liquid culture which has proven success and a lot of it.
but I'm quite new to lc, I know it's bad, but why not take the challenge.

my problems are, mycelium doesn't really float, more like dingle to the bottom..
I have kind of frisbee made of mycelium, which floats and looks like something I could pour out of an old bottle of milk..

it came from a clean culture, should I dump?
or just add some absinth, put on my pirate wood leg, chuck it and yell at the neighbors?


Extras: Filter Print Post Top
OfflineBloneDude
Stranger
Male User Gallery


Registered: 06/24/18
Posts: 8
Loc: Colorado
Last seen: 4 years, 6 months
Re: Recognizing and dealing with contamination [Re: tombonytom] * 1
    #25322887 - 07/12/18 07:01 PM (5 years, 7 months ago)

Hey yall,

I did  not want to create another thread since it appears many people have posted on this thread for help in identifying potential contamination. I am currently harvesting some fruit, but I noticed a few spots on a variety of cakes that are giving me some concern. I took some photos, and if I did this right they should load. If you could provide any assistance in letting me know if it could be something, or if I am just being over cautious it would be greatly appreciated!:cool:

Shot 1:


Shot 2:


Shot 3:


Shot 4:


Thanks for any help, and much love to you all!


--------------------
Selin


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 20 hours, 8 minutes
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: BloneDude] * 2
    #25323387 - 07/13/18 01:55 AM (5 years, 7 months ago)

It's just mycelium caused by the higher Co2 levels near the substrate and blue bruising from the substrate drying out.


--------------------


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25323608 - 07/13/18 07:25 AM (5 years, 7 months ago)

What I wanna know is, is everything we call trich actually trich? Or is most of it actually penicillin and we just can't tell the difference?


Extras: Filter Print Post Top
OfflineFunGuyZ
Psilly Rabbit


Registered: 12/13/16
Posts: 177
Loc: down the rabbit hole
Last seen: 4 years, 10 months
Re: Recognizing and dealing with contamination [Re: nube424] * 1
    #25339310 - 07/21/18 03:39 PM (5 years, 6 months ago)

Is this Trich- bad feeling



Extras: Filter Print Post Top
OfflineAnkhSaisRaKhamun
hermit
Registered: 03/15/15
Posts: 3
Last seen: 5 years, 6 months
Re: Recognizing and dealing with contamination [Re: Grey] * 1
    #25374770 - 08/08/18 11:17 AM (5 years, 6 months ago)

what happens when all growth stops and is dry?


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Recognizing and dealing with contamination [Re: AnkhSaisRaKhamun] * 1
    #25374809 - 08/08/18 11:36 AM (5 years, 6 months ago)

Then u throw it out. And start over.


Extras: Filter Print Post Top
OfflineIncreduLuck
Stranger
Registered: 08/11/18
Posts: 3
Last seen: 5 years, 6 months
Re: Recognizing and dealing with contamination [Re: nube424] * 1
    #25380873 - 08/11/18 03:35 AM (5 years, 6 months ago)

Hey guys, newb cultivator here.. This started and has been growing for like a week on the side of my container.. Do you think it's the aerial rhyzomorphs or something more serious? It's in strands and seem to grow upwards.
Picture:


Edited by IncreduLuck (08/11/18 12:48 PM)


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Recognizing and dealing with contamination [Re: IncreduLuck] * 1
    #25381370 - 08/11/18 10:17 AM (5 years, 6 months ago)

Upload the pic to the site. The little mountain icon over there when ur posting  ------->>


Extras: Filter Print Post Top
OfflineIncreduLuck
Stranger
Registered: 08/11/18
Posts: 3
Last seen: 5 years, 6 months
Re: Recognizing and dealing with contamination [Re: nube424] * 1
    #25381577 - 08/11/18 12:49 PM (5 years, 6 months ago)

Alright, I edited my previous post to include it. Thanks for the instructions!


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 20 hours, 8 minutes
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: IncreduLuck] * 1
    #25382565 - 08/11/18 09:54 PM (5 years, 6 months ago)

It's just mycelium, and it looks like there are some knots forming nearby :thumbup:


--------------------


Extras: Filter Print Post Top
OfflineIncreduLuck
Stranger
Registered: 08/11/18
Posts: 3
Last seen: 5 years, 6 months
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25384831 - 08/13/18 02:21 AM (5 years, 6 months ago)

Thanks for the advice! It's just weird to see mycelium grow up from the medium.


Extras: Filter Print Post Top
OfflineSamAtticus
Male User Gallery
Registered: 02/27/18
Posts: 175
Last seen: 6 months, 16 days
Re: Fantastic [Re: Kizzle] * 1
    #25421729 - 08/29/18 02:48 PM (5 years, 5 months ago)

Quote:

Kizzle said:
Quote:

SamAtticus said:
Amazingly helpful (and unnerving) index, especially useful are the pics of various contaminants. This must have taken months to put together. Fantastic work!

I must admit, though, I'm a little confused by this paragraph:

"Overlay - A common sign of Trichoderma is mycelium that colonizes over a casing layer or the vermiculite fruiting PF cakes have been rolled in. While mycelium can also create overlay, if your fruiting conditions have been adequate to prevent it, this can be one of the first signs you notice."

I'd love some clarification if and when you have the time or inclination.

Again, beautiful work! Thank you!



Thanks for pointing that out. In part it's confusing because I meant to upload some better pictures later but I also rewrote it so it makes more sense. Basically what I meant is when you notice mycelium growing over parts of the substrate you normally don't see it growing particularly in comparison with identically made substrates. When you have an actual casing layer you normally aim for it to not be colonized, and to mostly have rhizomorphs growing through it but then suddenly there's this patch growing over it and...
:scream:




Thank you so much for the clarification! Much appreciated.


--------------------

"I learn by going where I have to go." Theodore Roethke


Bod's AF Search Engine Tek


Extras: Filter Print Post Top
OfflineFriedmind
Stranger than thou
Male User Gallery
Registered: 07/20/18
Posts: 3
Last seen: 5 years, 3 months
Looking for verification. [Re: Kizzle] * 1
    #25436984 - 09/05/18 08:26 AM (5 years, 5 months ago)

First time for everything first grow, first flush, new member, and first post.  I am using rye berries in 110 quart mono tubs, with six 2 1/2 inch air exchange holes, three high and three low all stuffed with poly.
Everything has gone well until I put my myc in the mono tub. The first time I watered them I bruised one tub very badly and the other not so much. Thought I had contamination and freaked out. Now thanks to this site, I have learned to mist my tub’s and I have pins popping all over. Oh yes these are golden teachers on day 7 since the first pins appeared. I have a couple with fuzzy feet that I think is from poor fae, but with my poly and I fan them with each misting(up to 5 times/day if I’m home) I want a more experienced opinion. Thanks in advance and Thank You greatly for all the info on this site.


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Looking for verification. [Re: Friedmind] * 1
    #25444361 - 09/08/18 04:41 AM (5 years, 5 months ago)

It's very dry in there. Awesome job for ur first grow though!!!! Next time try making the poly on the bottom a lot tighter to hold the humidity in. If u dial in the tub right u shouldn't have to mist much.

Fuzzy feet are hard to avoid too. And it can happen from misting too. Ur on the right track tho man, I can tell uve been reading for awhile :smile:


Extras: Filter Print Post Top
OfflineFriedmind
Stranger than thou
Male User Gallery
Registered: 07/20/18
Posts: 3
Last seen: 5 years, 3 months
Re: Looking for verification. [Re: nube424] * 1
    #25450519 - 09/10/18 07:06 PM (5 years, 5 months ago)

Thank you. Glad to hear I’m doing something right lol. First flush gave me just over 5oz of dehydrated product between the two tubs. Somewhere I read to pluck em soon as the veil tears, so I ended up with a bunch of little ones. Towards the end of the flush I read that it doesn’t hurt to let them spread their caps, the last few were huge. Any input as to which way is best?


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Looking for verification. [Re: Friedmind] * 1
    #25451603 - 09/11/18 05:57 AM (5 years, 5 months ago)

They say for potency it's best to pick early. And f ur trying to avoid spores all over ur shrooms. Otherwise it doesn't hurt to let them open. If u want max weight without getting spores everywhere, pick the ones with open caps b4 they drop spores everywhere, and leave the rest till they start to open up.


Extras: Filter Print Post Top
OfflineFriedmind
Stranger than thou
Male User Gallery
Registered: 07/20/18
Posts: 3
Last seen: 5 years, 3 months
Re: Looking for verification. [Re: nube424] * 1
    #25478413 - 09/21/18 04:20 PM (5 years, 4 months ago)

At the end of my second flush I had spores drop, now 3 days later there is no new growth except for this (see picture) in one corner. Is my tub done for? Or can I cut out this corner and see what happens?


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Looking for verification. [Re: Friedmind] * 1
    #25478415 - 09/21/18 04:22 PM (5 years, 4 months ago)

Quote:

Friedmind said:
At the end of my second flush I had spores drop, now 3 days later there is no new growth except for this (see picture) in one corner. Is my tub done for? Or can I cut out this corner and see what happens?




No it's done. Put it outside, it's compost now.


Extras: Filter Print Post Top
OfflineWing
The Eye Tyrant
Male User Gallery


Registered: 04/25/11
Posts: 3,293
Last seen: 5 months, 28 days
Re: Looking for verification. [Re: Pastywhyte] * 1
    #25478872 - 09/21/18 06:50 PM (5 years, 4 months ago)

Quote:

Pastywhyte said:
Quote:

Friedmind said:
At the end of my second flush I had spores drop, now 3 days later there is no new growth except for this (see picture) in one corner. Is my tub done for? Or can I cut out this corner and see what happens?




No it's done. Put it outside, it's compost now.




:whathesaid:

Caught a case o' Trichoderma


--------------------
My Old Grow Logs



Extras: Filter Print Post Top
Invisibleorionstarseed
Stranger
 User Gallery
Registered: 09/22/18
Posts: 119
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25487094 - 09/25/18 02:29 AM (5 years, 4 months ago)

I have just tried to inoculate some jars with two different spore syringes  on the 20th and I have started to have Cobweb mold in them. Do you think my syringes are bad or what is going on? It seems the cobweb mold starts at about the 3-4 day mark and I don't believe it is the syringes because the mold started on the outside of the cakes and I inoculated them straight down into the substrate as I did not know I was supposed to inoculate towards the glass. What is going on and how can I prevent this? This is my first time doing this and so far it has been very dissapointing, I already have to chuck out 5 jars and I don't want to waste anymore solution or jars.



Edited by orionstarseed (09/25/18 02:31 AM)


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: [STICKY] Recognizing and dealing with contamination [Re: orionstarseed] * 1
    #25487400 - 09/25/18 07:32 AM (5 years, 4 months ago)

Sterilize ur jars longer. Cobweb mostly comes from not sterilizing well enough ime.

And yes, next time inoculate toward the glass so the water drips down the glass. It's easier to monitor growth.


Extras: Filter Print Post Top
Offlinealorac
Stranger
Registered: 09/30/18
Posts: 1
Last seen: 5 years, 4 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25502196 - 09/30/18 11:06 PM (5 years, 4 months ago)

Newbie question:

When I cut my shrooms to dry, I noticed blue-green everywhere I cut. Is this contamination? Is it bruising? Are these safe to eat?


Extras: Filter Print Post Top
OfflineWing
The Eye Tyrant
Male User Gallery


Registered: 04/25/11
Posts: 3,293
Last seen: 5 months, 28 days
Re: [STICKY] Recognizing and dealing with contamination [Re: alorac] * 1
    #25502202 - 09/30/18 11:12 PM (5 years, 4 months ago)

Its bruising and safe to eat


--------------------
My Old Grow Logs



Extras: Filter Print Post Top
OfflineGris
Stranger
Registered: 09/19/18
Posts: 1
Last seen: 4 years, 2 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Wing] * 1
    #25529114 - 10/11/18 08:17 AM (5 years, 4 months ago)

Getting started with agar and this pink dot showed up on one of my plates.

Anybody that could help identify and can I just cut it out of the plate ?





Extras: Filter Print Post Top
Invisibledmar
I'm a teapot

Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25531024 - 10/11/18 09:57 PM (5 years, 4 months ago)

On the list of non contaminants, you should include primordia. I had a bunch of yellow/brownish blobs form in my jars, and I thought it was some sort of bacterial infection. Come to find out it was primordia that eventually turned into knots and pinned. Probably just a rookie mistake, but since the point of this forum is helping rookies, it might be worth adding. Just a thought!


Extras: Filter Print Post Top
Offlinemayot02
Stranger
 User Gallery
Registered: 10/15/18
Posts: 1
Last seen: 5 years, 4 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25540338 - 10/15/18 07:56 PM (5 years, 4 months ago)



what do you think?
is there mold present?
this is after the 2nd surge of mushrooms.
there is a white fuzz growing that is new


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: [STICKY] Recognizing and dealing with contamination [Re: mayot02] * 1
    #25541137 - 10/16/18 05:04 AM (5 years, 3 months ago)

That's thrich. It'll be green shortly. Good eye. Most new people don't notice mold till it changes colors.


Extras: Filter Print Post Top
Invisibledmar
I'm a teapot

Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
Re: [STICKY] Recognizing and dealing with contamination [Re: nube424] * 1
    #25549934 - 10/19/18 10:59 AM (5 years, 3 months ago)



I got a nice flush out of these cakes, but this one had a good bit of blue bruising throughout the whole process. I dunked and rolled in verm for a second flush last night and about 14 hours later, I noticed that this patch where it was very blue is now looking awfully dark and almost gray. I think it's just bruising getting worse on that spot and i'm gonna keep an eye on it. Anyone more experienced think it looks more like a contam?


Extras: Filter Print Post Top
Offlinewhiskeyjack
Stranger
 User Gallery

Registered: 10/07/14
Posts: 15
Loc: Maryland
Last seen: 2 years, 7 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25642754 - 11/27/18 01:36 PM (5 years, 2 months ago)

Hi Folks,

  There's a long black thing in my grain jar. It's coming out of a particularly dark oat. I don't think it was there before. It looks like some kind of stroma. Admittedly, I haven't paid much attention to this batch, and it could've been there all along.

Also, I know the moisture is too high in these jars. My mistake. Please don't drag me.

Have you seen anything like this before?

Thanks!





--------------------


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: [STICKY] Recognizing and dealing with contamination [Re: whiskeyjack] * 1
    #25642764 - 11/27/18 01:41 PM (5 years, 2 months ago)

Could be ergot or just a rotted grain or was overly ripe when they picked it.


Extras: Filter Print Post Top
OfflineOatman2000
-=Outa Space=-
Male User Gallery


Folding@home Statistics
Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 1
    #25650686 - 12/01/18 01:24 PM (5 years, 2 months ago)

I love that you used one of my pics in this. :congrats:


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


Extras: Filter Print Post Top
Offlinefusselchen
Stranger
Registered: 11/04/18
Posts: 10
Last seen: 5 years, 1 month
Re: Recognizing and dealing with contamination [Re: Oatman2000] * 1
    #25667330 - 12/09/18 08:15 AM (5 years, 2 months ago)

thank you for this helpful sticky post! i'm always curious what kind of contamination there is.
a few days ago i had these mushrooms

and

when they started looking wet i first thought thats just some water because of my misting and will evaporate. but it didnt and a moderate smell of dirty laundry appeared and i threw them away.
i guess it was some kind of bacteria and my misting-mistake (too much? also on the fruit bodys)?


Extras: Filter Print Post Top
InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Recognizing and dealing with contamination [Re: fusselchen] * 1
    #25667768 - 12/09/18 11:46 AM (5 years, 2 months ago)

Bottom one is just a mutant..normal in MS...The white flecks are also pretty standard, but the wet patches likely bacterial infection, probably over misting initially, usually you'd see it on the stipe too


Extras: Filter Print Post Top
Invisibledmar
I'm a teapot


Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
Re: Recognizing and dealing with contamination [Re: fusselchen] * 1
    #25675318 - 12/12/18 06:51 PM (5 years, 2 months ago)

Quote:

fusselchen said:
thank you for this helpful sticky post! i'm always curious what kind of contamination there is.
a few days ago i had these mushrooms

and

when they started looking wet i first thought thats just some water because of my misting and will evaporate. but it didnt and a moderate smell of dirty laundry appeared and i threw them away.
i guess it was some kind of bacteria and my misting-mistake (too much? also on the fruit bodys)?



I had a MS grow that almost the entire tub was mutants with odd caps like that. Some of them grew with the cap completely inside out, some with the cap almost upside down, some just like yours is there. It's nothing to worry about.


Extras: Filter Print Post Top
Invisibledmar
I'm a teapot


Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
Re: Recognizing and dealing with contamination [Re: dmar] * 1
    #25683468 - 12/16/18 04:51 PM (5 years, 2 months ago)

I'm concerned that this might be trich, but due to my abundance of side pins, I'm pretty sure that my surface conditions were dry. So it could also be bruising from being dry. It's in a few places on the surface of this sub, but it's very faint. If it is trich, how long would it take for it to become more distinguishable? How fast does trich move once it's started dropping spores and turning green?


Extras: Filter Print Post Top
OfflineDtwiz
Stranger
I'm a teapot
Registered: 12/19/18
Posts: 6
Last seen: 3 years, 9 months
Re: Recognizing and dealing with contamination [Re: dmar] * 1
    #25689066 - 12/19/18 09:23 AM (5 years, 1 month ago)




This is my first time growing. I have four jars, inoculated with Puerto Rican.
One of them is showing two light green patches.
What can this be and what can i do?

Thanks in advance


Extras: Filter Print Post Top
Invisibledmar
I'm a teapot

Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
Re: Recognizing and dealing with contamination [Re: Dtwiz] * 1
    #25689444 - 12/19/18 12:34 PM (5 years, 1 month ago)

Quote:

Dtwiz said:



This is my first time growing. I have four jars, inoculated with Puerto Rican.
One of them is showing two light green patches.
What can this be and what can i do?

Thanks in advance



It's hard to tell with that picture, but if it's green, it's no good. If it's indeed contaminated, pressure cook it at 15 PSI for 90 minutes before you open it to throw it away. If it's kind of a faint color, wait a few days to be sure it's actually green.


Extras: Filter Print Post Top
OfflineGrowgrl
Female User Gallery


Registered: 03/25/18
Posts: 20
Loc: Hanging Gardens of Babylon
Last seen: 9 months, 24 days
Re: Recognizing and dealing with contamination [Re: dmar] * 1
    #25689552 - 12/19/18 01:30 PM (5 years, 1 month ago)

Okay, I'm totally frustrated here. Last year I had several successful grows: wbs and coir in monotubs. Life happened and I had to stop growing for a few months.  On 11/16/18 I inoculated a bunch of sterile wbs jars with agar. Everything was looking great.  They fully colonized and I made monotubs spawning the wbs with coco coir. I should've documented the date but I didn't (probably around 11/27). My mushrooms just aren't pinning.  They've been really dry due to the winter air and the only room I have with heat that I can grow in is heated by a wood burning stove.  So I've had to mist a lot and still I'm not seeing much condensation on the tubs like I did before. So now I'm thinking they're contaminated.  I don't know.  Anybody?


--------------------


Extras: Filter Print Post Top
InvisibleXnMe
Somth'n of a Somth'n
 User Gallery


Registered: 06/23/16
Posts: 1,278
Re: Recognizing and dealing with contamination [Re: Growgrl] * 1
    #25689858 - 12/19/18 04:15 PM (5 years, 1 month ago)

Quote:

Growgrl said:
Okay, I'm totally frustrated here. Last year I had several successful grows: wbs and coir in monotubs. Life happened and I had to stop growing for a few months.  On 11/16/18 I inoculated a bunch of sterile wbs jars with agar. Everything was looking great.  They fully colonized and I made monotubs spawning the wbs with coco coir. I should've documented the date but I didn't (probably around 11/27). My mushrooms just aren't pinning.  They've been really dry due to the winter air and the only room I have with heat that I can grow in is heated by a wood burning stove.  So I've had to mist a lot and still I'm not seeing much condensation on the tubs like I did before. So now I'm thinking they're contaminated.  I don't know.  Anybody?



Looks like bruising from pooling water, up FAE and stop misting for now.

Edit: at second look around the poly looks like really healthy myc, definitely up the fae !


--------------------
:volcano: Thanks too all :volcano:
        Best Tek List, click it


Edited by XnMe (12/19/18 04:17 PM)


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Recognizing and dealing with contamination [Re: XnMe] * 1
    #25690255 - 12/19/18 07:27 PM (5 years, 1 month ago)

Could be misting too much / too hard also. Just give it a light mist from a few feet above. High pressured mist can bruise mycelium


Extras: Filter Print Post Top
OfflineGrowgrl
Female User Gallery


Registered: 03/25/18
Posts: 20
Loc: Hanging Gardens of Babylon
Last seen: 9 months, 24 days
Re: Recognizing and dealing with contamination [Re: nube424] * 1
    #25690744 - 12/20/18 04:52 AM (5 years, 1 month ago)

Okay.  I'll up the fae and fewer mists from a higher level. Thanks for the replies.


--------------------


Extras: Filter Print Post Top
Invisiblenube424
Male

Registered: 12/03/17
Posts: 6,063
Re: Recognizing and dealing with contamination [Re: Growgrl] * 1
    #25690768 - 12/20/18 05:28 AM (5 years, 1 month ago)

Any time chica. When u mist, u want it to look like morning dew on the surface. Just tiny droplets that glisten. U only need to mist if it actually looks dry.


Extras: Filter Print Post Top
OfflineGrowgrl
Female User Gallery


Registered: 03/25/18
Posts: 20
Loc: Hanging Gardens of Babylon
Last seen: 9 months, 24 days
Re: Recognizing and dealing with contamination [Re: nube424] * 1
    #25693852 - 12/21/18 01:52 PM (5 years, 1 month ago)

Quote:

Okay, I'm totally frustrated here. Last year I had several successful grows: wbs and coir in monotubs. Life happened and I had to stop growing for a few months.  On 11/16/18 I inoculated a bunch of sterile wbs jars with agar. Everything was looking great.  They fully colonized and I made monotubs spawning the wbs with coco coir. I should've documented the date but I didn't (probably around 11/27). My mushrooms just aren't pinning.  They've been really dry due to the winter air and the only room I have with heat that I can grow in is heated by a wood burning stove.  So I've had to mist a lot and still I'm not seeing much condensation on the tubs like I did before. So now I'm thinking they're contaminated.  I don't know.  Anybody?




The morning after I posted this, I woke up to pins! lol


Edited by Growgrl (12/21/18 01:55 PM)


Extras: Filter Print Post Top
OfflineOctopus8
Aquatic Shape Shifter
I'm a teapot User Gallery

Registered: 02/05/19
Posts: 42
Loc: PNW Flag
Last seen: 4 years, 9 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25835850 - 02/24/19 11:18 PM (4 years, 11 months ago)

This is like the equivalent of sexual education and seeing all the pictures of STD's.

Great work!


Extras: Filter Print Post Top
OfflineBlacklight98
Myc hobbyists
 User Gallery

Registered: 12/09/18
Posts: 9
Last seen: 4 years, 9 months
Re: Recognizing and dealing with contamination *DELETED* [Re: Octopus8] * 1
    #25874301 - 03/14/19 05:03 PM (4 years, 10 months ago)

Post deleted by Blacklight98

Reason for deletion: Wasn’t edited


Extras: Filter Print Post Top
OfflineBlacklight98
Myc hobbyists
 User Gallery

Registered: 12/09/18
Posts: 9
Last seen: 4 years, 9 months
Re: Recognizing and dealing with contamination [Re: Blacklight98] * 1
    #25874355 - 03/14/19 05:34 PM (4 years, 10 months ago)

Could anyone tell me if this shroom looks okay there is white fuzzies around all the stocks i read where someone was saying its fine if there is white fuzz on the stock said it was just myc because of lack of air exchange but the discoloring at the bottom of the stem right when i Cropped it and i cut it open an it was white inside dont smell bad


Edited by Blacklight98 (03/14/19 05:39 PM)


Extras: Filter Print Post Top
OfflineCount of Sabugosa
Nerdy floater
Male User Gallery


Registered: 08/20/15
Posts: 939
Last seen: 8 months, 14 days
Re: Recognizing and dealing with contamination [Re: Blacklight98] * 1
    #25924873 - 04/09/19 11:40 AM (4 years, 10 months ago)

These mycobags were inoculated 3 weeks + 2 days ago and showed ZERO growth until last week. I mashed this bag with a couple of spots only because I thought "whatta heck, I've got nothing to lose anymore," since I was about to toss all the other bags. Out of 5, 2 survived. 1 shows a normal pace and healthy growth.

This one is sweating a lot. It's also colonizing faster (perhaps because of the mash?). I am not sure, because I see healthy myc inside, but also a thinner white veil and this to me seems excessive sweating. Thoughts?



--------------------
In Hebrew, the words "wine" and "secret" hold the same numerologic value. When wine comes in, secrets spill out. Do you think the person who said that knew mushrooms? When mushrooms come in... Is there anything beyond a secret?



Edited by Count of Sabugosa (04/09/19 11:41 AM)


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 20 hours, 8 minutes
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Count of Sabugosa] * 1
    #25931736 - 04/13/19 02:16 AM (4 years, 10 months ago)

It's hard to see what's going on in that bag. I'd give it some time to finish colonizing and check it out then when you can open the bag.


--------------------


Extras: Filter Print Post Top
OfflineCount of Sabugosa
Nerdy floater
Male User Gallery


Registered: 08/20/15
Posts: 939
Last seen: 8 months, 14 days
Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #25931803 - 04/13/19 03:55 AM (4 years, 9 months ago)

Here's 4.5 days after 100% break.



--------------------
In Hebrew, the words "wine" and "secret" hold the same numerologic value. When wine comes in, secrets spill out. Do you think the person who said that knew mushrooms? When mushrooms come in... Is there anything beyond a secret?



Extras: Filter Print Post Top
OfflineLand TroutM
Stranger
I'm a teapot User Gallery


Registered: 01/08/18
Posts: 3,144
Last seen: 14 hours, 43 minutes
Re: Recognizing and dealing with contamination [Re: Count of Sabugosa] * 1
    #25934594 - 04/14/19 04:14 PM (4 years, 9 months ago)

I’ve got a really nice pin set, but trich just showed up. What should I do Cut out the trich and let it flush?  Smother the trich in limestone powder?  Toss it in my worm bin and let it flush In There?


Extras: Filter Print Post Top
OfflinePusynality
Stranger
I'm a teapot User Gallery


Registered: 04/13/18
Posts: 10
Last seen: 4 years, 7 months
Re: Recognizing and dealing with contamination [Re: Land Trout] * 1
    #25935227 - 04/14/19 10:32 PM (4 years, 9 months ago)

So I already threw this jar away when I saw this,


But, what kind of contamination is this and what would have caused it?

I also have 5 other jars next to this one (like a few inches apart on the same table) when I discovered this particular jar. The other jars do not show anything similar, but would they be at risk for anything since they were so close to the contaminated jar (like maybe spread contamination airborne or something)?


Extras: Filter Print Post Top
Offlinelightrises
Stranger
 User Gallery
Registered: 02/07/19
Posts: 52
Last seen: 4 years, 6 months
Re: Recognizing and dealing with contamination [Re: Pusynality] * 1
    #26033231 - 06/04/19 08:33 PM (4 years, 8 months ago)

im guessing black mold? ive had a feew jars look exactly like that. I either get rid of the jar or deff isolate it from the others and out of your grow area


Extras: Filter Print Post Top
OfflineMycelial Messenger
Stranger
Registered: 10/29/18
Posts: 10
Last seen: 1 year, 8 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle] * 1
    #26038242 - 06/07/19 11:33 AM (4 years, 8 months ago)

Great article


Extras: Filter Print Post Top
OfflineShroomterd
42
Male User Gallery


Registered: 06/28/17
Posts: 220
Loc: washington
Last seen: 2 years, 6 months
Re: Recognizing and dealing with contamination [Re: Mycelial Messenger] * 1
    #26073132 - 06/25/19 02:35 PM (4 years, 7 months ago)

Very nice write up I’ll definitely be saving this


--------------------


Extras: Filter Print Post Top
OfflineZeak
Stranger
 User Gallery

Registered: 04/27/19
Posts: 31
Last seen: 3 years, 1 month
Re: Recognizing and dealing with contamination [Re: Shroomterd] * 1
    #26083857 - 07/01/19 09:36 AM (4 years, 7 months ago)

Seems like all of my grain jars have been contaminated. Trying to identify what with exactly and narrow down where could I have introduced it. It's very fluffy and appeared 4 days after ms injections in places of those injections. Rye was boiled for an hour and then PC'd for another hour. Any guesses?
😶




Extras: Filter Print Post Top
Offlinethe_mycologist
Stranger
Registered: 09/10/18
Posts: 4
Last seen: 4 years, 6 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Zeak] * 1
    #26086596 - 07/02/19 04:31 PM (4 years, 7 months ago)

My first real grow has been going really well until today when I noticed a couple of blue-ish spots in my tub. Do you think it's mold? If so, can I quarantine those spots somehow or should I go ahead and harvest what mushrooms have already grown and call it a bust?





Extras: Filter Print Post Top
OfflineFryMe
Stranger
Registered: 01/11/19
Posts: 11
Last seen: 4 years, 6 months
Re: [STICKY] Recognizing and dealing with contamination [Re: the_mycologist] * 1
    #26109935 - 07/15/19 03:47 PM (4 years, 6 months ago)

So I've been doing many bulk grows with cubes (B+, Treasure Coast, Golden Teachers, Blue Meanie, Koh Samui Super Strain, Pink Buffalo) and had great success on inoculating the jars in a SAB. Only contams I ever had was trich after many flushes of the tubs, so not big losses.

I decided to try out some Pan Cy's in agar dishes, inoculating directly from a syringe. All the plates I did turned to mold within 3 days. This is my first attempt with agar and my SAB was always nuked prior to working in it and I take many precautions, so I'm a bit confused. I used Bods methods for agar, following to a T and still got contams. Is there anything different with Pans? Should I just inoculate jars instead and then attempt to clone my Pans, since I know I don't get contams direct to jar?


Extras: Filter Print Post Top
OfflineCOGSx86
Stranger
Registered: 07/10/19
Posts: 8
Last seen: 4 years, 5 months
Re: [STICKY] Recognizing and dealing with contamination [Re: FryMe] * 1
    #26129106 - 08/09/19 08:57 PM (4 years, 6 months ago)

Is this contamination?

If so, anything I can do, any suggestions? 

First tub




Extras: Filter Print Post Top
OfflineSamAtticus
Male User Gallery

Registered: 02/27/18
Posts: 175
Last seen: 6 months, 16 days
Re: Recognizing and dealing with contamination [Re: Zeak] * 1
    #26143012 - 08/19/19 02:41 PM (4 years, 5 months ago)

I have the same problem. And the really bizarre part is, if you let it grow, it sprouts these weird-ass mushroom shaped' things.



--------------------

"I learn by going where I have to go." Theodore Roethke


Bod's AF Search Engine Tek


Extras: Filter Print Post Top
OfflineCOGSx86
Stranger
Registered: 07/10/19
Posts: 8
Last seen: 4 years, 5 months
Re: Recognizing and dealing with contamination [Re: SamAtticus] * 1
    #26146496 - 08/21/19 03:05 PM (4 years, 5 months ago)

Quote:

SamAtticus said:
I have the same problem. And the really bizarre part is, if you let it grow, it sprouts these weird-ass mushroom shaped' things.






sane, ate the mushrooms, and Im still alive :wink:


Edited by COGSx86 (08/21/19 03:05 PM)


Extras: Filter Print Post Top
OfflineSpiritualscience
Researcher
 User Gallery

Registered: 06/15/19
Posts: 305
Loc: USA
Last seen: 1 year, 8 months
Re: Recognizing and dealing with contamination [Re: COGSx86] * 1
    #26150213 - 08/23/19 09:48 PM (4 years, 5 months ago)

Man, a guy just shared this and I have never seen anything like it. What in the holy Hell is this???? Looks like some alien bacterial blobs. I just can't put a name to it.



--------------------
Jesus said to the ones who believed Him, If ye continue in my word, you shall know the truth, and the truth shall make you free.


Extras: Filter Print Post Top
Offlinepeanutbutterwaferb
Stranger
 User Gallery
Registered: 06/13/19
Posts: 22
Last seen: 4 years, 5 months
Re: Recognizing and dealing with contamination [Re: Spiritualscience] * 1
    #26152935 - 08/25/19 09:06 PM (4 years, 5 months ago)

Does anyone have a name for this?



Here's another:


Edited by peanutbutterwaferb (08/27/19 09:00 AM)


Extras: Filter Print Post Top
Offlineontheinternet
Stranger
 User Gallery
Registered: 07/19/19
Posts: 1
Last seen: 4 years, 4 months
Re: Recognizing and dealing with contamination [Re: peanutbutterwaferb] * 1
    #26165012 - 09/02/19 09:30 AM (4 years, 5 months ago)

Second monotub grow and got some weird growth. It's almost like there are mushrooms heads growing on the mushroom heads. I don't think it's a contaminate, but not sure.




Extras: Filter Print Post Top
OfflineSpiritualscience
Researcher
 User Gallery

Registered: 06/15/19
Posts: 305
Loc: USA
Last seen: 1 year, 8 months
Re: Recognizing and dealing with contamination [Re: ontheinternet] * 1
    #26165126 - 09/02/19 10:43 AM (4 years, 5 months ago)

Totally normal. It will not hurt anything.

Here is a first flush from a tub. It is on its sixth flush now. Only the first flush had the growths. It could be high humidity, minor response to bacteria, etc. Whatever it is, it has no negative effects on the shrooms.



--------------------
Jesus said to the ones who believed Him, If ye continue in my word, you shall know the truth, and the truth shall make you free.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Spiritualscience] * 1
    #26165128 - 09/02/19 10:46 AM (4 years, 5 months ago)

Quote:

Spiritualscience said:
Man, a guy just shared this and I have never seen anything like it. What in the holy Hell is this???? Looks like some alien bacterial blobs. I just can't put a name to it.






Just looks like normal SW penis envy blobs. They are common on the first flush.


Extras: Filter Print Post Top
OfflineSpiritualscience
Researcher
 User Gallery

Registered: 06/15/19
Posts: 305
Loc: USA
Last seen: 1 year, 8 months
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 1
    #26165157 - 09/02/19 11:07 AM (4 years, 5 months ago)

Dang, I think he chunked it already, but I will let him know for sure. He said he put it outside, but that was over a week ago.

Thanks for letting us know!


--------------------
Jesus said to the ones who believed Him, If ye continue in my word, you shall know the truth, and the truth shall make you free.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Spiritualscience] * 1
    #26165185 - 09/02/19 11:24 AM (4 years, 5 months ago)

That’s a shame. Blobs will get some decent size and are just as potent and good to eat.



Extras: Filter Print Post Top
OfflineReptilianBrain
Stranger
Registered: 09/01/19
Posts: 10
Last seen: 4 years, 2 months
Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 1
    #26165897 - 09/02/19 07:47 PM (4 years, 5 months ago)

Would it be cause for concern if I’ve noticed a slight fermentation/ earthy/ musty smell coming off my substrate 7 days into the spawn run? Initially I had the lid gasket sealed for 4 days before finding out that was a mistake—opened it up and there was a strong fermented smell, but not sure it wasn’t just from having misted H2O2 over the walls and substrate before sealing it up. Now 7 days into spawn run it seems like everything is okay, still notice a slight smell just sort of earthy/musty—nothing crazy.
At 4 days, I flipped the lid upside down for FAE, sprayed a bit more H2O2 and have since just been keeping a close eye on everything. Does thi sound okay by what I’ve described? Any suggestions on how to proceed? I’m not quite 100% colonized yet, temps around 75-78, humidity seems good. Did I dodge a bullet or Is it too early to tell? Below is a picture—seems okay? Anybody see something I’m missing?



Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 20 hours, 8 minutes
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: ReptilianBrain] * 1
    #26169223 - 09/04/19 05:37 PM (4 years, 5 months ago)

Quote:

ReptilianBrain said:
Would it be cause for concern if I’ve noticed a slight fermentation/ earthy/ musty smell coming off my substrate 7 days into the spawn run? Initially I had the lid gasket sealed for 4 days before finding out that was a mistake—opened it up and there was a strong fermented smell, but not sure it wasn’t just from having misted H2O2 over the walls and substrate before sealing it up. Now 7 days into spawn run it seems like everything is okay, still notice a slight smell just sort of earthy/musty—nothing crazy.
At 4 days, I flipped the lid upside down for FAE, sprayed a bit more H2O2 and have since just been keeping a close eye on everything. Does thi sound okay by what I’ve described? Any suggestions on how to proceed? I’m not quite 100% colonized yet, temps around 75-78, humidity seems good. Did I dodge a bullet or Is it too early to tell? Below is a picture—seems okay? Anybody see something I’m missing?





The fermentation odor was related to the lack of gas exchange. It's possible for a substrate to recover fully after fixing a problem like that but no guarantees.


--------------------


Extras: Filter Print Post Top
Offlinedrspastic
the good doctor
 User Gallery


Registered: 06/26/06
Posts: 147
Loc: bulgaria
Last seen: 1 year, 1 month
Re: Recognizing and dealing with contamination [Re: Kizzle]
    #26171753 - 09/06/19 01:08 AM (4 years, 5 months ago)

hi, latest batch of substrate has that sour smell soon after i spawned it. corn spawn very good and clean, straw boiled for over an hour.
only difference from last set of monos was i tried packing the layers of spawn and straw into clear plastic sacks stood in a empty mono to make large bricks then putting the bags on shelving.

I have read that gas exchange problems can cause smells but these are sour.

is there any cure or shall I dinv it outdoor to make another garden patch?

bricks are about 5kg each and are heavily spawned


--------------------
did you eat all that acid? you better pray to god there's some thorazine in that bag.


Extras: Filter Print Post Top
OfflineMotomushy
Stranger
 User Gallery
Registered: 09/16/19
Posts: 3
Last seen: 4 years, 4 months
Re: Recognizing and dealing with contamination [Re: drspastic]
    #26191576 - 09/16/19 11:56 AM (4 years, 4 months ago)

Can anyone tell me if this is contaminated? The get lighter in color as they grow


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Motomushy]
    #26192379 - 09/16/19 05:11 PM (4 years, 4 months ago)

Quote:

Motomushy said:
Can anyone tell me if this is contaminated? The get lighter in color as they grow





Those are too dry. You need another layer of mp tape over the bottom holes. No contams that I can see.


Extras: Filter Print Post Top
OfflineMotomushy
Stranger
 User Gallery
Registered: 09/16/19
Posts: 3
Last seen: 4 years, 4 months
Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #26192930 - 09/16/19 10:43 PM (4 years, 4 months ago)

Thank you! I sprayed a lil water mist in the tub and they have a blueish hue now... That's normal right?


Extras: Filter Print Post Top
Invisiblemyc.kind.o.lady
Female

Registered: 09/17/19
Posts: 4
Re: Recognizing and dealing with contamination [Re: Kizzle]
    #26208652 - 09/25/19 01:31 PM (4 years, 4 months ago)

Hello all :-) Amazing original post, thanks for all the great info!! MUSH APPRECIATED!!

This is my first time posting anything so I'm apologizing in advance if not proper...

I've been having this issue and i'm losing sleep:confused:...

colonized jars look and smell great - when they are spawned to bulk and after consolidation this is what is happening around day 7-10 after fruiting:





(I could try taking better pics if necessary)

My worst fear is that this is ASPERGILLUS - Can anyone confirm what it is?

**I've been retracing steps and thinking that my pasteurization technique is prob faulty - vac bags filled with bulk sub and cooked in water (170 for 2hrs) i deff over filled and over wet bulk probably causing to get too hot on outside and not enough in center ??
*** I've also had the window open (no AC and super hot in here) could this have been a factor?

if not aspergillus ...Could these blocks be saved? Maybe soaking in mild bleach solution??

I tossed out about 10 (2L) blocks last time as soon as I saw yellow (they were just starting to fruit)....so sad ... but noticed that it only affected the top - under liner everything looked and smelled healthy....

now I have 8 new tubs that are affected and hoping I can save if possible

If not able to save - no problem - health and safety first and also the MAIN thing I need to know is What am I doing wrong and how can I avoid this in the future???

Thanks so much for your time :heart:


Extras: Filter Print Post Top
Offlineflyingcap
Explorer
 User Gallery
Registered: 10/08/19
Posts: 8
Last seen: 2 months, 8 days
Re: Recognizing and dealing with contamination [Re: myc.kind.o.lady]
    #26243011 - 10/10/19 03:30 PM (4 years, 4 months ago)

Got a few ugly Bettys growing. Reading through some prior threads it can be over misting and lack of FAE or a genetic mutation. Can anyone advise the exact cause and solution for future growing knowledge ? Appreciate the knowledge folks.



Extras: Filter Print Post Top
Offlinetomcards
Male


Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 11 months, 6 days
Re: Recognizing and dealing with contamination [Re: flyingcap]
    #26289644 - 10/31/19 11:45 AM (4 years, 3 months ago)

Thanks to Kizzle for this fantastic, informative thread.

Can anyone identify the beast or beasties that contaminated this tub?




Extras: Filter Print Post Top
Offlinesolarflash
Stranger
Registered: 11/27/19
Posts: 1
Last seen: 3 years, 7 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #26349508 - 11/27/19 08:44 PM (4 years, 2 months ago)

The first 4 times I ever tried the pftek method I was successful.  I enjoyed the fruits of my labor.  The next 6 times all ended in fast spreading green contamination.  The cakes were fully colonized and put into sanitized fruiting chamber.  I fanned them daily and used the same care as the first 4 successful batches.  Soon after the contamination overtook all the cakes.      Batch after batch until I wasted 6 spore syringes.  I gave up for a couple of years but am going to try again.    This time using a sterilized rye grain kit. 
Many posters here are very adamant about not using a kit but how bad can these kits be.  What’s worse?  Spending $50 on a sterilized kit or wasting syringe after syringe and not knowing exactly where you went wrong?  Appreciate any constructive advice.


Extras: Filter Print Post Top
InvisibleZombiWurm
Stranger

Registered: 10/15/18
Posts: 644
Re: Recognizing and dealing with contamination [Re: tomcards]
    #26366470 - 12/07/19 08:44 AM (4 years, 2 months ago)

Cobweb in the bottom left corner?


Extras: Filter Print Post Top
Offlinedrewman522
Stranger
 User Gallery
Registered: 12/08/19
Posts: 3
Last seen: 3 years, 12 days
Re: Recognizing and dealing with contamination [Re: ZombiWurm]
    #26368810 - 12/08/19 10:48 AM (4 years, 2 months ago)

Thank you for this detailed write up! I'm brand new to this forum, apologies if this reply breaks any rules. And sorry to ask what may be the obvious but hoping someone more experienced than me can confirm that this cake is contaminated and should just be trashed? OR is it worth continuing?

This is our first grow and we've been following the PF Tek Easy BRF Cakes found here: https://www.shroomology.org/forums/topic/13173-pf-tek-easy-brf-cakes-mushroom-growing-method/

Started on 11/3 with 8 cakes and already had to dispose of 6 of them because of visible contams and really no white mycelium.

Any guidance is greatly appreciated!

https://imgur.com/bP32ldr



Edited by drewman522 (12/08/19 10:50 AM)


Extras: Filter Print Post Top
InvisibleZombiWurm
Stranger

Registered: 10/15/18
Posts: 644
Re: Recognizing and dealing with contamination [Re: drewman522]
    #26368828 - 12/08/19 11:00 AM (4 years, 2 months ago)

That's the wrong jar for BRF cakes. You won't ever get the cake out of that jar. Anyway it looks fine to me. Stick to wide mouth half pint jars. I've always used the regular mouth half pints but it seems the short ones colonize faster


Extras: Filter Print Post Top
InvisiblePsilotyl
נָז֓יר
Male User Gallery

Registered: 08/30/19
Posts: 469
Loc: עולם
Re: Recognizing and dealing with contamination [Re: tomcards]
    #26368935 - 12/08/19 12:05 PM (4 years, 2 months ago)

Quote:

tomcards said:
Thanks to Kizzle for this fantastic, informative thread.

Can anyone identify the beast or beasties that contaminated this tub?







hard too really see there. Even so...who knows :/ I don’t think it’s penicillium. It could be aspergillus, chaetomium, trichoderma, maybe cladosporium...but clad is usually darker looking than what’s in your pic. Sadly there are so many ā€œgreensā€ that can eff shit up it’s hard for most of us to say what’s what with certainty


--------------------
ā€Ž×©×œ×•× וְאור | PEACE & LIGHT


Extras: Filter Print Post Top
InvisibleZombiWurm
Stranger

Registered: 10/15/18
Posts: 644
Re: Recognizing and dealing with contamination [Re: Psilotyl]
    #26369350 - 12/08/19 04:02 PM (4 years, 2 months ago)

Quote:

Psilotyl said:
Quote:

tomcards said:
Thanks to Kizzle for this fantastic, informative thread.

Can anyone identify the beast or beasties that contaminated this tub?







hard too really see there. Even so...who knows :/ I don’t think it’s penicillium. It could be aspergillus, chaetomium, trichoderma, maybe cladosporium...but clad is usually darker looking than what’s in your pic. Sadly there are so many ā€œgreensā€ that can eff shit up it’s hard for most of us to say what’s what with certainty




Yeah it's really hard to tell. You could culture it but even then without a scope you are stuck with observing how it colonized the agar to determine what it is and how the mycelium grows.


Extras: Filter Print Post Top
Offlinetomcards
Male


Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 11 months, 6 days
Re: Recognizing and dealing with contamination [Re: ZombiWurm]
    #26370574 - 12/09/19 09:50 AM (4 years, 2 months ago)

Thanks for the feedback. 

My sterile technique was sorely lacking.  There were probably all manner of nasty beasties in the substrate.  After one flush, I harvested 83.3 cracker-dry grams of Golden Teacher.  I dumped the rest of the substrate.  I kick myself as I fantasize about how much that tub could have yielded.

Since then, I've learned much.  This won't happen again.


Tom


Extras: Filter Print Post Top
Offlinedrewman522
Stranger
 User Gallery
Registered: 12/08/19
Posts: 3
Last seen: 3 years, 12 days
Re: Recognizing and dealing with contamination [Re: ZombiWurm]
    #26370733 - 12/09/19 11:19 AM (4 years, 2 months ago)

Realized I replied to the wrong post, but thanks for the reply! Wow so you think no concerns with contams based on those pics?

Thanks for the note on the jars - these are wide mouth as directed but realized too late exactly what you're saying... may end up having to break them to get the cakes out.


Extras: Filter Print Post Top
InvisiblePsilotyl
נָז֓יר
Male User Gallery

Registered: 08/30/19
Posts: 469
Loc: עולם
Re: Recognizing and dealing with contamination [Re: tomcards]
    #26370877 - 12/09/19 12:49 PM (4 years, 2 months ago)

Quote:

tomcards said:
Thanks for the feedback. 

My sterile technique was sorely lacking.  There were probably all manner of nasty beasties in the substrate.  After one flush, I harvested 83.3 cracker-dry grams of Golden Teacher.  I dumped the rest of the substrate.  I kick myself as I fantasize about how much that tub could have yielded.

Since then, I've learned much.  This won't happen again.


Tom




That’s still not bad! There’s always next time, as lame as that sounds lol


--------------------
ā€Ž×©×œ×•× וְאור | PEACE & LIGHT


Extras: Filter Print Post Top
OfflineA_Fun_Guy_Learns
Stranger
 User Gallery
Registered: 03/09/19
Posts: 1
Last seen: 2 years, 5 months
Re: Recognizing and dealing with contamination [Re: Psilotyl]
    #26372091 - 12/09/19 11:54 PM (4 years, 2 months ago)

Is this the meany greeny, or just metabolites?




Extras: Filter Print Post Top
InvisiblePsilotyl
נָז֓יר
Male User Gallery

Registered: 08/30/19
Posts: 469
Loc: עולם
Re: Recognizing and dealing with contamination [Re: A_Fun_Guy_Learns]
    #26372913 - 12/10/19 10:58 AM (4 years, 2 months ago)

Quote:

A_Fun_Guy_Learns said:
Is this the meany greeny, or just metabolites?







Looks like that’s probably fine. Keep an eye on it and see if it turns & you’ll get a definitive answer pretty quickly


--------------------
ā€Ž×©×œ×•× וְאור | PEACE & LIGHT


Extras: Filter Print Post Top
OfflineMushToLearn
Stranger
 User Gallery
Registered: 01/06/20
Posts: 1
Last seen: 4 years, 1 month
Re: Recognizing and dealing with contamination [Re: Psilotyl]
    #26418832 - 01/06/20 07:06 AM (4 years, 1 month ago)

Hi folks, newbie here.
I've just managed my first grow of 5 BRF cakes. I've done one flush so far and have dunked and setup for flush No.2.
Past day or so i've noticed a very slight musky smell coming from my FC which made me head here to try get some info on possible contams.
After reading a lil i am leaning towards a possible Trich contam but im not sure as i obviously lack the experience.
I can still smell the Mycelium as on the first flush but now with a very slight musky smell. I don't see any obvious mold spots but after reading about the white growth that comes before Trich spores i have a feeling this is what i am faced with.
If it wasn't for the slight smell i would have thought these cakes were fine.
Posted some pics so if anyone would like to help out a newbie i'd appreciate the advice. Cheers.







Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 20 hours, 8 minutes
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: MushToLearn]
    #26419784 - 01/06/20 06:19 PM (4 years, 1 month ago)

All you can do is wait and see.


--------------------


Extras: Filter Print Post Top
Offlinedrewman522
Stranger
 User Gallery
Registered: 12/08/19
Posts: 3
Last seen: 3 years, 12 days
Re: Recognizing and dealing with contamination [Re: Kizzle]
    #26422048 - 01/07/20 09:47 PM (4 years, 1 month ago)

Hi all, thanks to @zombiwurm for replying to my last post. Have some updated photos (and figured out how to include images properly:cool:) so I though i'd post again. These BRF cakes are just over 2 months old and i think looking really good except for a few possible contams in each. Can anyone help identify and is there any cause for concern or should we proceed with the dunk and fruiting?



Thank you!


Extras: Filter Print Post Top
Offlinesnowytips
Stranger
 User Gallery
Registered: 01/21/20
Posts: 2
Last seen: 4 years, 20 days
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #26453014 - 01/26/20 06:47 AM (4 years, 21 days ago)

After a couple of weeks or so of mushrooms growing on the outside of the casing, however on the top where no mushrooms are growing some green mold has started to appear... any suggestions of what it is or to remedy the situation?? :confused:



Extras: Filter Print Post Top
OfflineD123456
Stranger
 User Gallery
Registered: 01/28/20
Posts: 1
Last seen: 4 years, 19 days
Pink [Re: Kizzle]
    #26456943 - 01/28/20 02:17 PM (4 years, 19 days ago)



Please can anyone help me, does anyone know if my crop is contaminated


Extras: Filter Print Post Top
OfflineTynuk
Stranger
Registered: 02/18/19
Posts: 1
Last seen: 1 year, 9 months
Re: Pink [Re: D123456]
    #26469563 - 02/05/20 01:26 AM (4 years, 12 days ago)

Hi everyone,
Was hoping if someone could give me an advise. I have 2 spots of dark green mold in my monotub (wbs and coco coir). I hace read on here that I can try to cut them out and cover the spots with salt. Anyone tried doing that before?

Thanks in advance!





Extras: Filter Print Post Top
Offlinekillmatrixolme
Stranger

Registered: 02/09/20
Posts: 12
Last seen: 8 months, 17 days
Re: Recognizing and dealing with contamination [Re: Kizzle]
    #26480287 - 02/11/20 02:14 PM (4 years, 5 days ago)

Can i eat this mushroom?
i think the white color is bold. they are contaminated right?




Extras: Filter Print Post Top
OfflineSynKyd
ctrl-alt-delite
Male User Gallery


Registered: 09/27/13
Posts: 1,554
Loc: ૐ
Last seen: 2 years, 10 months
Re: Recognizing and dealing with contamination [Re: killmatrixolme]
    #26480980 - 02/11/20 08:04 PM (4 years, 5 days ago)

Quote:

killmatrixolme said:
Can i eat this mushroom?
i think the white color is bold. they are contaminated right?







Those are fine.  Scrape the verm off the bottom and enjoy :cheers:


--------------------

New inclusive poop emojis from Apple!

:shpongle::shpongle::shpongle::shpongle::shpongle:



Extras: Filter Print Post Top
Offlineendlessnessagain
Stranger
Registered: 01/25/20
Posts: 22
Last seen: 3 years, 11 months
Re: Recognizing and dealing with contamination [Re: SynKyd]
    #26483155 - 02/13/20 06:01 AM (4 years, 3 days ago)

Is there anything like this thread but specifically for agar contamination, to help identify them?


Extras: Filter Print Post Top
InvisibleNigel Stephens
 User Gallery
Registered: 02/21/20
Posts: 103
Re: Recognizing and dealing with contamination [Re: endlessnessagain]
    #26503091 - 02/25/20 12:27 PM (3 years, 11 months ago)

Do I have anything to worry about here, are they safe to eat?
I think it’s pseudomonas or verticillium, stems are hollow too

This is 2nd flush after dunking substrate / floating harvest tek.
Have since removed lid for FAE and drying but should I just harvest and toss? Obvs then bleach my whole grow area :smile:

Thank-you in advance for your time and advice







Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 20 hours, 8 minutes
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: Nigel Stephens]
    #26503268 - 02/25/20 02:09 PM (3 years, 11 months ago)

They're still edible. It looks like at an earlier time they were drenched and got waterlogged.


--------------------


Extras: Filter Print Post Top
InvisibleRyeHumor
MasterProcrastinator
Male


Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
2 out of 18 BRF Jars... So long Fare Well? [Re: Kizzle]
    #26503422 - 02/25/20 03:33 PM (3 years, 11 months ago)

Howdy

To give a background on the grow started 2-16:

Purchased a grow kit online, "pre-sterilized" jars and all. Coupled with my poor technique looks like of the 18 pint jar BRFs: (3)nonstarters (1)definitely contam'd and thrown out (green mold) (12)looking good (2)I suspect contam and have isolated.

Here are the two in question: The first was a result of a slipped plunger that colonized half the jar almost within the first week. Looked good until sectioning appeared, the second growth looks white if not as bright as the first portion. What really bugs me is that there appears to be no rhizomorphic growth at the edges to match the brighter section. Here are some photos of that jar. The black marks are in the 3rd photo are left over from pen marks I was using to track progress.



And here is the second jar in question:



I'm 95% sure that these two are ready to be tossed, however I would love to hear any thoughts to the contrary as well as a possible identity of the contamination if anyone can provide.
Thank you!


Edited by RyeHumor (02/25/20 06:30 PM)


Extras: Filter Print Post Top
OfflineFunjunk1121
Stranger
 User Gallery
Registered: 02/03/20
Posts: 12
Last seen: 3 years, 10 months
Re: Recognizing and dealing with contamination [Re: RyeHumor]
    #26509979 - 02/29/20 04:22 PM (3 years, 11 months ago)

Hey what are your thoughts on what I have going on here?



Extras: Filter Print Post Top
OfflineFunjunk1121
Stranger
 User Gallery
Registered: 02/03/20
Posts: 12
Last seen: 3 years, 10 months
Re: Recognizing and dealing with contamination [Re: Funjunk1121]
    #26510147 - 02/29/20 05:59 PM (3 years, 11 months ago)

Better pics



Extras: Filter Print Post Top
Offlinebeammeup90
Stranger
 User Gallery

Registered: 04/08/19
Posts: 2
Loc: Central California Flag
Last seen: 3 years, 9 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #26520965 - 03/06/20 04:59 PM (3 years, 11 months ago)

How bad is the contamination and is it a toss the whole tub situation?

Higher res https://files.shroomery.org/files/20-10/355223956-QdaqgFl.jpg

This is the original grow:[image]Original https://imgur.com/a/BCOGcnf[/image]

This is the current grow that I think may be contaminated. Any thoughts on the difference in size and shape? https://files.shroomery.org/files/20-10/363943527-IMG_20200307_180811.jpg


Edited by beammeup90 (03/07/20 08:52 PM)


Extras: Filter Print Post Top
OfflineKookaburraMan
not a jock
I'm a teapot User Gallery


Registered: 12/12/19
Posts: 20
Last seen: 3 years, 5 months
Re: [STICKY] Recognizing and dealing with contamination [Re: beammeup90]
    #26525452 - 03/09/20 12:52 PM (3 years, 11 months ago)

Hey shroomers! I think these are pre-pin bumps but as a second-time grower, I just wanna make sure everything is honky dory.  Thanks for reviewing and possibly giving me some feedback! Love y'all!





--------------------


Extras: Filter Print Post Top
OfflineCowboy Buckie
General
Male User Gallery

Registered: 05/18/14
Posts: 93
Loc: Texas Flag
Last seen: 2 years, 10 months
Re: Recognizing and dealing with contamination [Re: tomcards]
    #26528840 - 03/11/20 09:52 AM (3 years, 11 months ago)

I assume you’re talking about the cpl green spots? How much has it changed since you posted?


Extras: Filter Print Post Top
Offlinebeammeup90
Stranger
 User Gallery

Registered: 04/08/19
Posts: 2
Loc: Central California Flag
Last seen: 3 years, 9 months
Re: Recognizing and dealing with contamination [Re: Cowboy Buckie]
    #26530127 - 03/12/20 12:18 AM (3 years, 11 months ago)

The color didn' change much. Primarily white with a little yellow, brown, and a tiny bit of green. I harvested the first flush today. It may have been a bit early but i had a mix of buttons to fully developed  mushrooms. What's most interesting is how they differ from the grow that the spores came from. The prior grow had Longer skinny stalks and relatively small caps. This grow is filled with short stocky robust mushrooms. [url=http://files.shoomery.org/forums/thumbs/20-11/399103535-thumb_MG_0263.jpg[/image][/url]      [url=http://files.shoomery.org/forums/thumbs/20-11/399104362-thumb_IMG_20200311-113634.jpg[/image]


--------------------
I've had poison oak every month for five years


Extras: Filter Print Post Top
OfflineLREAM
Registered: 11/13/19
Posts: 59
Loc: Pinner City
Last seen: 2 years, 1 month
Re: Recognizing and dealing with contamination [Re: beammeup90]
    #26534789 - 03/14/20 01:00 PM (3 years, 10 months ago)



Towards the bottom right corner I have a blue discoloration. Its a bit hard
to tell since I just noticed this morning.
This is my first grow and I just pushed my first fruting bin in a few days ago.
Want to know if I should trash and start over or if I'm dealing with something other than blue mold



In front of the pin



It does seem a bit wet on the surface so I cracked the lid more for FAE.
If anyone can help identify or has any suggestions that would be great, before I end up trashing it if it gets worse. Thanks!


Edited by LREAM (03/14/20 01:03 PM)


Extras: Filter Print Post Top
Offlinesquidward18
Stranger

Registered: 01/04/20
Posts: 19
Last seen: 3 years, 10 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
    #26535466 - 03/14/20 08:11 PM (3 years, 10 months ago)

Any ideas on what this might be? Looks like there's a little pool of gunk.


Some of the stocks have developed these bubbles:

Thanks in advance for any help!


Extras: Filter Print Post Top
OfflineUncaged_Goose
Stranger
Registered: 03/28/20
Posts: 1
Last seen: 3 years, 8 months
Re: [STICKY] Recognizing and dealing with contamination [Re: squidward18]
    #26563145 - 03/28/20 12:42 PM (3 years, 10 months ago)

Long time lurker, first time poster! I'm in the middle of my first ever grow and I'm worried about contamination.

I definitely made some mistakes - 7 out of 10 jars were possibly contaminated so I only fruited 3 of them. The grow looked good but one of my mushrooms was a little slimy under the cap. All the veils were broken, but it didn't seem like they dropped spores yet.

There is some blue that looks like it could be mold on the dirt on top of my cakes (they didn't quite colonize fully). Pictures for everything here: http://imgur.com/gallery/2xhk6Ai

Is this OK? Should I abort and try again? Thank you in advance :smile:


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Re: [STICKY] Recognizing and dealing with contamination [Re: Uncaged_Goose]
    #26563272 - 03/28/20 01:41 PM (3 years, 10 months ago)

the fruits are fine I'm not sure if that's mold or bruising, if it's green it's contaminated


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineElusively
Stranger
 User Gallery

Registered: 04/25/17
Posts: 11
Last seen: 3 years, 7 months
Re: [STICKY] Recognizing and dealing with contamination [Re: cronicr]
    #26563896 - 03/28/20 06:20 PM (3 years, 10 months ago)

Using pasty’s easy agar tek —> bods easy oat prep tek

I feel like this look good, but they were a little fluffy in the glad mini rounds and still look kinda fluffy.

Anyone have some time to reassure my fragile ego?!  Btw, thank in advance for the abundance of info on this site/community, y’all are Effin amazing!



Extras: Filter Print Post Top
Offlinejbgtaa
extraterrestrial
Male User Gallery


Registered: 06/09/19
Posts: 1,785
Last seen: 3 years, 3 months
Re: [STICKY] Recognizing and dealing with contamination [Re: Elusively]
    #26564432 - 03/29/20 01:47 AM (3 years, 10 months ago)

Does look a lil fluffy but you’re gonna need a week or two more to really tell unless it’s really tams out. How long did that take to grow and how many transfers away from the (I assume) spore plate was the culture you use for inoculation?


--------------------
If the thunder don't get ya, the lightning will.
In another time's forgotten space, your eyes looked through your mother's face.
Trade List
Forever giving away prints. PM at anytime for a free print.


Extras: Filter Print Post Top
OfflineElusively
Stranger
 User Gallery

Registered: 04/25/17
Posts: 11
Last seen: 3 years, 7 months
Re: [STICKY] Recognizing and dealing with contamination [Re: jbgtaa]
    #26564749 - 03/29/20 08:17 AM (3 years, 10 months ago)

Thanks for the reply sir.  This is my first time w/ agar and a bulk tek, so I’m just feeling it out, doing sort of a ā€œtrial runā€ to see how my technique is and what kind of learning curve I’m working on.    Just giving you some context before I answer your questions:

-that growth is about 5-6 days after innoc those grain jars.

-I used about 1/10th of a wedge in each jar from what I (very noobishly and very arbitrarily) decided was the healthiest of the 4 agar plates I had.

-I did zero transfers, that’s my first go at agar and like I said, I arbitrarily decided that one looked healthy so eff it let’s keep rolling, was my thought process.

-I still have the 3 other agar plates, they’re about 3.5 weeks old now and the growth appears to have stopped, could I still do a transfer with those?


Extras: Filter Print Post Top
OfflineInthepit
Aum Mani Padme Hum
Male User Gallery


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 month, 4 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: Elusively] * 1
    #26568473 - 03/31/20 05:40 AM (3 years, 10 months ago)

Thank for this awesome reference and helpful place!
I could use some help to correct whatever I did to get this black mold.
My first guess is the from the water I was misting with.
Possibly I could avoid the black mold by misting with bleach?
:mushdance:   
A little history of the tubs; first they were too wet, haven't dialed in the proportions yet.
Then I took the lids and put them sideways, but I believe the tubs got too dry. Then I started misting heavily.
So basically over correction all around, noob style!

This looks like mold. Black Mold? LOL
My question is if I caught it early
would this How to Make 150 ppm Chlorine Solution by c10 to get rid of it.
And I wonder how I got it, there's lots of mold here in the tropics.
No one drinks the tap water here!


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


Edited by Inthepit (03/31/20 07:15 AM)


Extras: Filter Print Post Top
OfflineBikerfool
Your Local Edgelord
Male

Registered: 11/21/05
Posts: 1,577
Last seen: 6 months, 3 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: Inthepit]
    #26570251 - 04/01/20 12:36 AM (3 years, 10 months ago)

Inthepit, there's no saving that, throw it away ASAP.

If I ever find any contams it goes instantly into the trash. Even a tiny speck of green mold, I'll pick what's there and throw it out. It's not worth "trying to get one more flush." Trace back through your sterile technique and change or eliminate suspect practices.

My #1 piece of advice to a new grower is to get a flow hood.


--------------------
Just an angsty teen contributing to the pubs decline with contentless posts.


Extras: Filter Print Post Top
OfflineInthepit
Aum Mani Padme Hum
Male User Gallery


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 month, 4 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: Bikerfool]
    #26570390 - 04/01/20 04:48 AM (3 years, 10 months ago)

Quote:

Bikerfool said:
Inthepit, there's no saving that, throw it away ASAP.

If I ever find any contams it goes instantly into the trash. Even a tiny speck of green mold, I'll pick what's there and throw it out. It's not worth "trying to get one more flush." Trace back through your sterile technique and change or eliminate suspect practices.

My #1 piece of advice to a new grower is to get a flow hood.




Thankyou for the reply.
I wonder if I should use bleach mist anyway as this area has lots of mold.
:hatsoff:


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


Extras: Filter Print Post Top
OfflineGalactic_Grape
Stranger
 User Gallery

Registered: 03/07/18
Posts: 21
Last seen: 6 months, 20 days
Re: [STICKY] Recognizing and dealing with contamination [Re: Inthepit] * 1
    #26570923 - 04/01/20 10:57 AM (3 years, 10 months ago)

This is day 8 of fruiting, no pins yet. Pf classic with wbs spawn / damion 50/50 coir tek. Woke up this morning and saw these fluffy little balls but I cannot tell if these are knots or the start of trich. Separated this tub from the rest. I’ll know if it goes green by tomorrow but looking for opinions / might be useful to someone down the road if it turns out to be trich so you can see what it looks like before it goes totally green. Spot in question is in the middle of the tub above the crack.

Crack in the sub is from the bottom of the tub bowing down when I had them stacked and then pressed up flat when I put it on the ground.



Extras: Filter Print Post Top
OfflineBikerfool
Your Local Edgelord
Male

Registered: 11/21/05
Posts: 1,577
Last seen: 6 months, 3 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: Inthepit]
    #26571048 - 04/01/20 11:58 AM (3 years, 10 months ago)

Quote:

Inthepit said:
Quote:

Bikerfool said:
Inthepit, there's no saving that, throw it away ASAP.

If I ever find any contams it goes instantly into the trash. Even a tiny speck of green mold, I'll pick what's there and throw it out. It's not worth "trying to get one more flush." Trace back through your sterile technique and change or eliminate suspect practices.

My #1 piece of advice to a new grower is to get a flow hood.




Thankyou for the reply.
I wonder if I should use bleach mist anyway as this area has lots of mold.
:hatsoff:




I wouldn't recommend spraying your grow with bleach even if it is a weak solution. If will kill the mushrooms along with all other types of mold.

Having a dirty/ moldy environment is a disadvantage, but if you have good sterile technique and FAE you'll still be successful.


--------------------
Just an angsty teen contributing to the pubs decline with contentless posts.


Edited by Bikerfool (04/01/20 12:15 PM)


Extras: Filter Print Post Top
Offlinejudenfisch
Stranger
Male User Gallery
Registered: 03/23/16
Posts: 37
Loc: Solothurn
Last seen: 18 hours, 43 minutes
Re: [STICKY] Recognizing and dealing with contamination [Re: Bikerfool] * 1
    #26576664 - 04/04/20 03:57 AM (3 years, 10 months ago)

hello everyone!

the mycelium on the stem of the tallest mushrooms looks strange to me.

the greenish/ blueish spot i was afraid of first is bruising i think...

what do u think?? is it contaminated there? i am very afraid, would be nice to have another opinion!



--------------------
everything is information


Extras: Filter Print Post Top
Invisiblemurderlabz
RIP Stoneman
I'm a teapot User Gallery

Registered: 05/18/19
Posts: 551
Loc: The Multiverse
Re: [STICKY] Recognizing and dealing with contamination [Re: judenfisch] * 1
    #26578245 - 04/04/20 10:18 PM (3 years, 10 months ago)

Quote:

judenfisch said:
hello everyone!

the mycelium on the stem of the tallest mushrooms looks strange to me.

the greenish/ blueish spot i was afraid of first is bruising i think...

what do u think?? is it contaminated there? i am very afraid, would be nice to have another opinion!






Hard to see, I have had deep greenish bruising before that freaked me out. Do you have a "sterile" Q-Tip? Swab it, if it transfer to the Q-Tip it's spores from a mould.


Extras: Filter Print Post Top
Offlineartkng
Stranger
Registered: 04/05/20
Posts: 10
Last seen: 3 years, 9 months
Re: [STICKY] Recognizing and dealing with contamination [Re: murderlabz] * 1
    #26580457 - 04/05/20 08:45 PM (3 years, 10 months ago)

Lots of great info being shared, much needed for new growers. Thanks for the wisdom!


Extras: Filter Print Post Top
OfflineGoombala
Professional Clown
I'm a teapot User Gallery

Registered: 02/06/20
Posts: 24
Last seen: 1 year, 6 months
Re: [STICKY] Recognizing and dealing with contamination [Re: artkng]
    #26581542 - 04/06/20 11:52 AM (3 years, 10 months ago)

Hey guys does this look sketchy? It has a smell like bread dough which is the only thing scaring me so far.

Spawned on 3/30


Extras: Filter Print Post Top
InvisibleR3dstaffy
šŸ’¤
Male


Registered: 01/25/20
Posts: 115
Re: [STICKY] Recognizing and dealing with contamination [Re: Goombala]
    #26582170 - 04/06/20 05:01 PM (3 years, 10 months ago)

.


Edited by R3dstaffy (07/23/20 04:13 PM)


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery


Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: [STICKY] Recognizing and dealing with contamination [Re: R3dstaffy]
    #26585900 - 04/08/20 09:04 AM (3 years, 10 months ago)

:fffuck:  :badtrip:  :shiiiiit:  :cannotunsee:  :fuckthisshit:

FML-- I just dropped a quart jar full of sporulating trich in my kitchen, releasing a cloud of goddamn spores into the air-- how fucked am i?


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

Spore Trading List


Extras: Filter Print Post Top
InvisibleRyeHumor
MasterProcrastinator
Male


Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
Re: [STICKY] Recognizing and dealing with contamination [Re: R3dstaffy] * 1
    #26586892 - 04/08/20 05:19 PM (3 years, 10 months ago)

With the exception of that one black spot in the middle photo I would say you're looking fine.

I just birthed my pf jars a week ago and the initial growth looked about like that 2-3 days after noc.

any ideas as to what that black portion in the middle jar is?

If you can get in closer on it, might help.

I was in a similar situation with my jars. I obsessed over it, but really unless you are in a hurry to reuse the jar there's not much you can do other than watch it colonize and see if it expresses obvious signs of contamination.


Edited by RyeHumor (07/26/20 11:00 PM)


Extras: Filter Print Post Top
InvisibleRyeHumor
MasterProcrastinator
Male


Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
    #26586902 - 04/08/20 05:24 PM (3 years, 10 months ago)

Quote:

coAsTal said:
:fffuck:  :badtrip:  :shiiiiit:  :cannotunsee:  :fuckthisshit:

FML-- I just dropped a quart jar full of sporulating trich in my kitchen, releasing a cloud of goddamn spores into the air-- how fucked am i?




Might want to wipe down the area/vacuum, especially if you have a hepa filter in your unit. I don't think there's much more you can do.

I was just reading a thread with some input from Bhod and Mad that seemed on a similar subject (cleaning contaminated tubs).

It seems the best thing you can do regardless is just to make damn sure that your spawn is clean AF.

For your future projects, as long as your spawn is fully colonized and contam free, the rest of the process is not sterile and the myc should be strong enough to resist contamination at that point.

So despair not :wink:

I've heard a lot about bleach bombing places, from what I've gathered on this site from more experienced posters is that this is really not necessary. Probably more like soapy water washdown and vacuuming.


Extras: Filter Print Post Top
InvisibleR3dstaffy
šŸ’¤
Male


Registered: 01/25/20
Posts: 115
Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
    #26587300 - 04/08/20 08:38 PM (3 years, 10 months ago)

.


Edited by R3dstaffy (07/23/20 04:14 PM)


Extras: Filter Print Post Top
Offlineonoff
Stranger
 User Gallery
Registered: 03/08/20
Posts: 2
Last seen: 3 years, 8 months
Re: [STICKY] Recognizing and dealing with contamination [Re: R3dstaffy]
    #26588833 - 04/09/20 01:07 PM (3 years, 10 months ago)

Thanks for the information everyone :smile:
Another first-timer here.
Do you think this may be in vitro pinning or metabolites?
Not sure whether I should fruit these or not, any opinions welcomed!
Thanks.



Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery


Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
    #26589036 - 04/09/20 02:56 PM (3 years, 10 months ago)

Thank you for the kind words- - I let the area settle for a couple hours then sprayed/misted the area with cleaner, let is get wet, then cleaned it up.
I've already had two months of contamination issues, which is why I was so bummed by this, so maybe in my old age I'm no longer able to properly make clean spawn and am destined to swear off mushrooms forever :fishsmack:

Onward and upward!


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

Spore Trading List


Extras: Filter Print Post Top
OfflineDezGWest7
JacQ


Registered: 04/09/20
Posts: 29
Last seen: 1 year, 8 months
Re: [STICKY] Recognizing and dealing with contamination [Re: onoff]
    #26589537 - 04/09/20 07:26 PM (3 years, 10 months ago)

What's up guys. First timer here. I inoculated WBS March 27 in 3 Jars with GT. The thing is, i see NO activity in 2 of them and the one that I do see activity, I believe its probably contaminated but I'm not so certain. Here is a pic of it. I left it alone originally for the 1st 9 days or so until i decided to check it out and saw what the picture shows but smaller and yes, growth in those 2 different spots. I'm not sure where I f'ed up but *note- this WBS preparation did not include coffee grounds or gypsum. I rinsed , picked the floating grain off, rinsed until clear - soaked 20+ hours - simmered stovetop - then strained , spread out and left to dry a little over 2 hours(I recall it wasn't 100% dry when I decided to pack in the Jar for PS 120min Sterilization. Was it wet wet ? Noo. It just had enough moisture for me to notice when the grains were touching the inside of the jar after loading up. After the PC I allowed it to cool down more than 16 hours from my recollection & then I did the whole sterilize GT syringe etc inoculation of jars.





Feedback is appreciated.


Extras: Filter Print Post Top
Offlinefolder52
Stranger
Registered: 04/11/20
Posts: 6
Last seen: 10 months, 2 days
Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
    #26593587 - 04/11/20 01:55 PM (3 years, 10 months ago)

Hi everyone! So I got my first 4 cakes recently. There are those green-blue spots everywhere (including pins). It was here since colonization process. Is that contamination? Should I burn my entire house now?





Extras: Filter Print Post Top
InvisibleRyeHumor
MasterProcrastinator
Male


Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
Re: [STICKY] Recognizing and dealing with contamination [Re: folder52]
    #26593700 - 04/11/20 03:01 PM (3 years, 10 months ago)

Quote:

folder52 said:
Hi everyone! So I got my first 4 cakes recently. There are those green-blue spots everywhere (including pins). It was here since colonization process. Is that contamination? Should I burn my entire house now?








I'm sure someone more experienced will chime in. But to me it appears like bruising, which is not something to worry about.


Extras: Filter Print Post Top
OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house Flag
Last seen: 7 hours, 53 minutes
Trusted Cultivator
Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
    #26594188 - 04/11/20 08:16 PM (3 years, 10 months ago)

:whathesaid:


--------------------

Crack's Tek's and Shit



Extras: Filter Print Post Top
OfflineBig_Dub
I'm just some guy
Male


Registered: 01/12/11
Posts: 2,700
Loc: Los Angeles Flag
Last seen: 1 day, 5 hours
Re: [STICKY] Recognizing and dealing with contamination [Re: Crackatoa]
    #26596648 - 04/12/20 08:34 PM (3 years, 9 months ago)

Yeah. Looks like bruising from where you have been handling it


--------------------
split_by_nine said:
click me you fuck


do the right thing


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery


Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: [STICKY] Recognizing and dealing with contamination [Re: Big_Dub]
    #26599833 - 04/14/20 08:45 AM (3 years, 9 months ago)

So a question fellas---
(sorry for the long post, but I'm really stumped.)

I've had some limited results with a 50/50 blend of hydrated straw pellet matter and fine verm in half-pint jars with both SAT cubes and ATL#7 colonizing well with no evident contams.
I also have a Zapotecorum pint WBS that appears not contammed that has colonized 100%. :mushroomgrow:

BUT-- I've had contam issues in all other pints of straw mix, WBS, and barley I've done (a dozen at least), and 100% contamination in all quart-sized jars of straw mix, Barley, and also WBS.

It's been driving me crazy to figure out what the hell is going on, because I have been using a SAB and making sure my agar wedges were good-- but I've had over a dozen-- probably closer to 20 jars actually-- contam with trich in the past 2 months.

I PC'd these jars for no less than 90 minutes after full-heat pre-steaming for 10 to 15 minutes prior to putting the weight on my pressure cooker to get all the dry air out. There are no leaks from the lid.

Initially I thought I must be introducing bacteria into the jars when nocking up, but something just wasn't right, because I have been going nuts to keep things clean w/liberal alcohol and proper SAB methods.

So I decided to PC some jars of the straw and verm mix, and some barley, and just keep them sealed straight out of the PC (with foil on still) set aside to see if somehow I wasn't getting full sterilization.

Well, the results are in: Every jar has contaminated within 2 weeks despite being PC'd and never opened.:banghead:

How the fuck could this happen?!

I'm using a very small PC-- it'll only fit 2 quarts in it angled on their sides-- and I had a quarter taped to the weight to help weigh it down some. I wait until it has cooled to open and remove the lid. I have the lid holes covered with micropore tape.
But somehow, despite the weight shaking a bit indicating full inside pressure, I'm clearly not sterilizing these quarts even with 2 hours under pressure.

I can only surmise that the temps/pressures aren't getting to the levels they need to be at to sterilize the quarts.

So now that I've wasted 10 weeks watching all my efforts fail, can you please help me figure out why I'm failing-- I should have been sitting on ounces of dried shrooms by now, and instead I have 4 half-pint straw jars colonized with mycelium, and one pint of Zap.

I'm making some more straw mix now, and will put two additional quarters on the weight to further increase pressure and will try to make sure I hit 120 minutes at full pressure as a start-- but otherwise I'm not sure what to do...


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

Spore Trading List


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery


Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
    #26602939 - 04/15/20 01:01 PM (3 years, 9 months ago)

I added the extra quarters and PC's for 1:45 hr yesterday-- will nock up with existing SAT half-pint that's 100% colonized on Friday-- I'm hoping the addition of the weight will have increased pressure and heat to fully sterilize the two qurts of pellet straw/verm.

Will update


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

Spore Trading List


Extras: Filter Print Post Top
InvisibleRyeHumor
MasterProcrastinator
Male


Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
    #26603006 - 04/15/20 01:26 PM (3 years, 9 months ago)

Just a thought. You might benefit from buying some of those autoclave indicators.

I believe the sponsors (mushroomsupply) have both polymer pellets in glass and the autoclave tape.

It indicates when sterilization temperatures have been reached.

Not sure if they take into account the amount of time spent at temp but if it does that could be really useful.

As I've seen mentioned before, venting your pc for ~10min to make sure you have nothing but steam in there is also important.

If you have a mixture of air and water you will not be reaching 121C even if you're up to pressure.


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery


Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
    #26603278 - 04/15/20 03:20 PM (3 years, 9 months ago)

I don't put the lid on until it's already at full boil, then I always vent at full heat and full steam (after the lid lock has popped up) for 10 to 15 minutes with the lid on before putting the primary weight on the valve--


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

Spore Trading List


Extras: Filter Print Post Top
InvisibleRyeHumor
MasterProcrastinator
Male


Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
    #26603304 - 04/15/20 03:37 PM (3 years, 9 months ago)

Given that you were seeing contamination inside the pc without opening the lid...

I would guess that either it wasn't reaching the pressure you thought it was.

In which case you can just add weight the way you described (but at a certain point you don't want to exceed the limitations of your pc)

Alternatively you can try and confirm with one of those indicators.

and third, it would be nice to have a pressure gauge... but on second thought modifying a pressure vessel is not the safest of ideas. So I can't actually recommend it.

Is $80 for a 23qt Presto PC with a pressure gauge outside of the budget?

I know it's on the more expensive side for this hobby, but I just picked one up and it's been great so far.

----------------

Another thought, what are you using to filter the gas exchange?


Edited by RyeHumor (04/15/20 03:37 PM)


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery


Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
    #26603353 - 04/15/20 04:02 PM (3 years, 9 months ago)

I've been laid off because of this stupid fucking virus bullshit, and have no spare money to use on this hobby (without my wife castrating me)-- so upgrading equipment is a 0% chance for the foreseeable future I'm afraid. :runaway:

The three quarters on the weight didn't pop the overpressure valve on the lid, so I think it's OK-- still astonished that I was contamming straight from the PC-- I'm looking forward to these two to see if they perform better with the weights on.:peace:


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

Spore Trading List


Extras: Filter Print Post Top
InvisibleRyeHumor
MasterProcrastinator
Male


Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
    #26603399 - 04/15/20 04:27 PM (3 years, 9 months ago)

Roger that. And sorry to hear it man.

One more exp you could try is to make a no pour and put something obviously contaminated in it to see if it still grows after.

But that's not necessarily that great due to the fact that spores and bacteria can be more or less resistant.

If it fails, it would tell you that something was up for sure though.


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery


Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
    #26603450 - 04/15/20 04:47 PM (3 years, 9 months ago)

No worries-- I appreciate the advice. REEEEALLLY hoping I can get this shit figured out so I can get some shit growing


Extras: Filter Print Post Top
InvisibleRyeHumor
MasterProcrastinator
Male


Registered: 02/21/20
Posts: 210
Loc: Yeast Of Knowhere
Re: [STICKY] Recognizing and dealing with contamination [Re: coAsTal]
    #26603505 - 04/15/20 05:17 PM (3 years, 9 months ago)

yeah I know the feeling.

dealing with contamination aint no fun.

Hang in there man,

if at first you fail, try again.

if you fail again, go office space on the son of a 
:beatingtodeath:

Just kidding. You'll get there :cheers:


Extras: Filter Print Post Top
Offlinefolder52
Stranger
Registered: 04/11/20
Posts: 6
Last seen: 10 months, 2 days
Re: [STICKY] Recognizing and dealing with contamination [Re: RyeHumor]
    #26604993 - 04/16/20 06:56 AM (3 years, 9 months ago)

An update for my previous post: green-blue something is still here, also some mutants :smile: How are we doing?







Extras: Filter Print Post Top
Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
Re: [STICKY] Recognizing and dealing with contamination [Re: folder52]
    #26605246 - 04/16/20 08:54 AM (3 years, 9 months ago)

Looks dry


--------------------
How I do glass dishes


Extras: Filter Print Post Top
Offlinefolder52
Stranger
Registered: 04/11/20
Posts: 6
Last seen: 10 months, 2 days
Re: [STICKY] Recognizing and dealing with contamination [Re: poisoned]
    #26605512 - 04/16/20 10:43 AM (3 years, 9 months ago)

Quote:

poisoned said:
Looks dry



Is it OK to eat?


Extras: Filter Print Post Top
Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
Re: [STICKY] Recognizing and dealing with contamination [Re: folder52]
    #26605657 - 04/16/20 11:31 AM (3 years, 9 months ago)

Most definitely!


--------------------
How I do glass dishes


Extras: Filter Print Post Top
Offlinemxmushroom
Stranger
Registered: 04/16/20
Posts: 1
Last seen: 3 years, 9 months
Re: Recognizing and dealing with contamination [Re: poisoned]
    #26605728 - 04/16/20 12:08 PM (3 years, 9 months ago)

Hey deadheads!
Just harvested a batch of p. cubensis, and sadly my batch has a bad case of cottonweb mildew. Are these still edible? There are no unusual smells or colors, and none of the mushies have been altered in any way except some small amounts of the mildew on the stems.
Probably going to eat one and see what happens.
I'd love some advice though! I'm a first time grower!
Cheers all


Extras: Filter Print Post Top
InvisiblecoAsTal
Friend
 User Gallery


Registered: 04/04/06
Posts: 2,970
Loc: 8a
Re: Recognizing and dealing with contamination [Re: mxmushroom]
    #26605746 - 04/16/20 12:19 PM (3 years, 9 months ago)

Welcome to the Shroomery-- I'd just take a toothbrush to it to scrub off the funk, but I can't imagine it would be a problem


--------------------
I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

Spore Trading List


Extras: Filter Print Post Top
Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
Re: Recognizing and dealing with contamination [Re: coAsTal]
    #26605896 - 04/16/20 01:41 PM (3 years, 9 months ago)

Can you show us some pics? Sounds like myc to me


--------------------
How I do glass dishes


Extras: Filter Print Post Top
OfflineEvolvedr79
Stranger
Registered: 04/21/19
Posts: 4
Last seen: 3 years, 23 days
Re: Recognizing and dealing with contamination [Re: poisoned]
    #26610360 - 04/18/20 12:13 PM (3 years, 9 months ago)

Hello everyone. This tub is one of three that came from a WBS spawn. Substrate is CVG. The other two tubs produced 2500 wet grams with a 12.5:1 wet to dry ratio. This last tub looks to have metabolites to me which would indicate a contamination but I am not sure which it is. I've only ever dealt with Trich in my previous tubs. The yellow coloring is abnormal to me.

Can someone please tell me if this tub is contaminated and if so, what is it?

Thanks.






Extras: Filter Print Post Top
OfflineDezGWest7
JacQ


Registered: 04/09/20
Posts: 29
Last seen: 1 year, 8 months
Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
    #26610619 - 04/18/20 01:52 PM (3 years, 9 months ago)

Quote:

DezGWest7 said:
What's up guys. First timer here. I inoculated WBS March 27 in 3 Jars with GT. The thing is, i see NO activity in 2 of them and the one that I do see activity, I believe its probably contaminated but I'm not so certain. Here is a pic of it. I left it alone originally for the 1st 9 days or so until i decided to check it out and saw what the picture shows but smaller and yes, growth in those 2 different spots. I'm not sure where I f'ed up but *note- this WBS preparation did not include coffee grounds or gypsum. I rinsed , picked the floating grain off, rinsed until clear - soaked 20+ hours - simmered stovetop - then strained , spread out and left to dry a little over 2 hours(I recall it wasn't 100% dry when I decided to pack in the Jar for PS 120min Sterilization. Was it wet wet ? Noo. It just had enough moisture for me to notice when the grains were touching the inside of the jar after loading up. After the PC I allowed it to cool down more than 16 hours from my recollection & then I did the whole sterilize GT syringe etc inoculation of jars.





Feedback is appreciated.




Since no one gave me any feedback I waited to see how it would progress and I suppose it answered any questions I had. This is how it looks as of today


Edited by DezGWest7 (04/18/20 01:53 PM)


Extras: Filter Print Post Top
OfflineWillieFunGuy
Stranger

Registered: 02/11/20
Posts: 34
Last seen: 5 months, 26 days
Re: [STICKY] Recognizing and dealing with contamination [Re: DezGWest7]
    #26622479 - 04/23/20 02:12 PM (3 years, 9 months ago)

Dude...looks perfect to me. I understand your trepidation though. I’m fairly new myself. Contams happen to the best of us. Keep it moving. Good job!


Extras: Filter Print Post Top
OfflineCrackatoa
Stranger in a strange land
I'm a teapot User Gallery


Registered: 03/31/19
Posts: 5,401
Loc: Over by your Mama's house Flag
Last seen: 7 hours, 53 minutes
Trusted Cultivator
Re: [STICKY] Recognizing and dealing with contamination [Re: WillieFunGuy] * 1
    #26622501 - 04/23/20 02:18 PM (3 years, 9 months ago)

Give her a shake šŸ‘


--------------------

Crack's Tek's and Shit



Extras: Filter Print Post Top
Offlinenevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 24 days
Re: [STICKY] Recognizing and dealing with contamination [Re: Crackatoa]
    #26623669 - 04/23/20 11:47 PM (3 years, 9 months ago)

Is this some stage of knots/primordia I've never noticed before or am I growing something freaky?


--------------------
My LAGM 2020 Journal


Extras: Filter Print Post Top
Invisiblemurderlabz
RIP Stoneman
I'm a teapot User Gallery

Registered: 05/18/19
Posts: 551
Loc: The Multiverse
Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita]
    #26623681 - 04/23/20 11:56 PM (3 years, 9 months ago)

Quote:

nevereataamanita said:
Is this some stage of knots/primordia I've never noticed before or am I growing something freaky?





Looks like it's building up Metabolites getting ready for war with something.


Extras: Filter Print Post Top
Offlinenevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 24 days
Re: [STICKY] Recognizing and dealing with contamination [Re: murderlabz]
    #26623689 - 04/24/20 12:03 AM (3 years, 9 months ago)

Damn, guess I'll keep an eye out and be ready to toss the shoebox. Here's a pic of the whole surface, I haven't spotted the contam yet.


--------------------
My LAGM 2020 Journal


Extras: Filter Print Post Top
Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita]
    #26624749 - 04/24/20 01:15 PM (3 years, 9 months ago)

Probably a bacteria, you won't really see it and you'll still get fruits.


--------------------
How I do glass dishes


Extras: Filter Print Post Top
Offlinenevereataamanita


Registered: 09/08/19
Posts: 121
Last seen: 2 months, 24 days
Re: [STICKY] Recognizing and dealing with contamination [Re: poisoned]
    #26624761 - 04/24/20 01:19 PM (3 years, 9 months ago)

I've been wondering, is it a bad idea to clone or print from a bacterial grow? Is that shit going to get passed on or could you get away from it on agar?


--------------------
My LAGM 2020 Journal


Extras: Filter Print Post Top
Offlinepoisoned
untitled
I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 3 months
Re: [STICKY] Recognizing and dealing with contamination [Re: nevereataamanita] * 1
    #26624801 - 04/24/20 01:39 PM (3 years, 9 months ago)

usually fruits won't host other organisms on the inside.


--------------------
How I do glass dishes


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Boomr Bag   Mushroom-Hut Liquid Cultures   Kraken Kratom Kratom Capsules for Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Capsules   Bridgetown Botanicals Bridgetown Botanicals


Similar ThreadsPosterViewsRepliesLast post
* Panaeolus Cyanescens TheShroomHermit 3,211 12 01/21/03 02:56 PM
by Psychoslut
* Tricks for recognizing overlay TaoinShrrom 456 3 07/17/03 12:43 PM
by Teon
* cauliflower?? soochi 1,857 3 10/04/02 10:42 AM
by soochi
* cauliflower question fungusflip 967 2 08/21/03 04:20 PM
by fungusflip
* Smelt Jars for contamination. I was taken to ER!!!
( 1 2 3 4 5 6 all )
JayBin 42,963 110 01/15/21 06:15 AM
by bodhisatta
* . *DELETED*
( 1 2 all )
Fd3000 5,843 22 08/22/02 11:08 PM
by Zen Peddler
* P. mexicana strains contaminated mjshroomer 1,438 3 07/26/04 03:04 PM
by Pinback
* Contamination Identification & Subjugation NutsackRucksack 13,871 4 11/19/20 09:38 PM
by Goat 2020

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
427,638 topic views. 16 members, 93 guests and 33 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.123 seconds spending 0.018 seconds on 13 queries.