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InvisibleSmartattackS
C'mon man
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Registered: 12/21/18
Posts: 3,286
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Re: Recognizing and dealing with contamination [Re: Big_Dub]
    #27473295 - 09/18/21 07:56 PM (2 months, 10 days ago)

Quote:

Big_Dub said:
I have an amscope. They are like the kind you would find in an undergraduate program




Yeah here as well. Im definitely going to be in the market for something more in the $2000 range in the future but for now I have plenty to see with a $450 scope.


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OnlineBig_DubS
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Re: Recognizing and dealing with contamination [Re: Smartattack]
    #27473359 - 09/18/21 08:54 PM (2 months, 10 days ago)

I think mine was like 200 new.

I need to pull it out of the box!


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OfflineSpaceBabyS
alchemist
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Registered: 11/01/20
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Re: Recognizing and dealing with contamination [Re: Big_Dub]
    #27473455 - 09/18/21 10:24 PM (2 months, 10 days ago)

alright guys

i taped the holes till final verdict.
obviously i aint comfy if i taped the holes. didnt put it out in 79F i. case it is not fucked. dont want to make a nice space heh for bacteria n shit.

I look forward to Smart w the gentle input. :laugh2:

all the same gt clone tub at 9 et

and if its what i did 4.5g of this evening touring it ain't no big loss. i get better visual with 9-lb hammer weed















--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

Cult Queen:

Another Day's Work:


MUSHBOY'S SHROOM TEA TEK


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InvisibleSmartattackS
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Re: Recognizing and dealing with contamination [Re: SpaceBaby]
    #27473461 - 09/18/21 10:29 PM (2 months, 10 days ago)

Well, as long as the rest fills in like that...:awesomenod:


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OfflineSpaceBabyS
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Re: Recognizing and dealing with contamination [Re: Smartattack]
    #27473513 - 09/18/21 11:13 PM (2 months, 10 days ago)

Quote:

alright guys
i taped the holes till final verdict.
obviously i aint comfy if i taped the holes. didnt put it out in 79F i. case it is not fucked. dont want to make a nice space heh for bacteria n shit.

the one photo of the huatla shoe is not part of my question...

Quote:

Smartattack said:
Well, as long as the rest fills in like that...:awesomenod:







so that means the tub is ok and i can untape the holes, right? :blazed:


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

Cult Queen:

Another Day's Work:


MUSHBOY'S SHROOM TEA TEK


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OfflineSpaceBabyS
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Re: Recognizing and dealing with contamination [Re: SpaceBaby]
    #27473636 - 09/19/21 12:37 AM (2 months, 9 days ago)

what's up here, please?



i'll edit in some more later. i

Enigma LC streak plate test. Rear view


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

Cult Queen:

Another Day's Work:


MUSHBOY'S SHROOM TEA TEK


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Offlinebenis12
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Re: Recognizing and dealing with contamination [Re: SpaceBaby]
    #27474014 - 09/19/21 09:29 AM (2 months, 9 days ago)

Got a ton of plates across multiple cultures that are starting to look like this.  I'm thinking this is what it looks like when a mold has gotten embedded into the myc to the point where I need to trash them and start again.  I know not all good myc growth has to be rhyzo, but these don't even look like healthy tomentose growth to me.  Any thoughts?

Been battling some type of subtle mold for the better part of six months now.  Frustrating.





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Offlineshuna
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Registered: 03/07/21
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Re: Recognizing and dealing with contamination [Re: benis12]
    #27474029 - 09/19/21 09:46 AM (2 months, 9 days ago)

Been battling that too.  I reckon it is bacteria causing the myc to grow funny, making it look like mold.

I've finally got round to trying some jars with a little layer of brf+verm, or in my case home blended brown rice and coir mixed to field capacity and in the PC to sterilise. Waiting for supplies to arrive for brf+verm.

So there's 8 jars:
2x small rice size wedge from myc on agar
2x josex poke from myc on agar
2x spores from print direct
2x drop or two from syringe made from a print

The wedges are taking off already, so fingers crossed they will spread on top of the cake and leave behind the bacteria.

Constant agar transfers were getting me nowhere. If this works then..:awedance:


Edited by shuna (09/19/21 09:59 AM)


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OfflineOra
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Re: Recognizing and dealing with contamination [Re: shuna]
    #27474074 - 09/19/21 10:26 AM (2 months, 9 days ago)

Did you change your agar recipe? Maybe too many nutes


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OfflineJXAllen
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Registered: 08/06/21
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Re: Recognizing and dealing with contamination [Re: Ora]
    #27475301 - 09/20/21 09:54 AM (2 months, 8 days ago)

I set this jar aside a week ago because it has what I believe to be yeast contamination, based on other pictures I've looked at. Well, I decided to take a look at it today before deciding if I should chuck it and it's pinning!

So, here are my questions....how bad is yeast? Can I still try and top fruit the jar? If I top fruit, I rinse out the verm layer and fill it back with CVG, correct? Should I add any water or would that just flush the yeast all over?



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OfflineDj_karma_magnet

Registered: 04/03/19
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27479951 - 09/23/21 10:37 PM (2 months, 5 days ago)

I have 12 of these bags and some of them are starting to turn blue. Was going to break and shake but think that might make it worse. People on Reddit are telling me it’s not contamination if it’s blue but I’m quickly learning people on Reddit are generally fucking morons. I got even more suspicious when I read this thread and saw that the way the mycelium forms can be telling of contamination as well. These 2 are the worst but there’s a couple more, was going to break and shake this weekend but thinking these just need to be tossed. Would like some opinions, the strain is cubensis b+ and I inoculated on 9/6





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InvisibleSmartattackS
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Re: Recognizing and dealing with contamination [Re: Dj_karma_magnet]
    #27479960 - 09/23/21 10:42 PM (2 months, 5 days ago)

I would definitely shake and see how they go. Can't see in the pics but don't look terrible.


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OfflineDj_karma_magnet

Registered: 04/03/19
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Re: Recognizing and dealing with contamination [Re: Smartattack]
    #27479985 - 09/23/21 11:11 PM (2 months, 5 days ago)

Thanks, managed to get a couple more while breaking and shaking








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OfflineCapMeh
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Re: Recognizing and dealing with contamination [Re: Dj_karma_magnet]
    #27480849 - 09/24/21 03:47 PM (2 months, 4 days ago)

There's no obvious contam I can see from the pictures, I would shake, let fully colonize, and then introduce fruiting conditions. You'll probably get fruits if you let it do it's thang!


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OfflineNekonation
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Registered: 02/22/21
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27481719 - 09/25/21 01:38 PM (2 months, 3 days ago)

All the following pics are cultivated from the same syringe of JMF. Three different shoe boxes. No signs of contamination otherwise. Are they ok?







--------------------
:mushroom2: Why bitch about things? If you can't change them there's no point. If you can change them then bitching just wastes time you could use for making changes. :grin:


Edited by Nekonation (09/25/21 01:40 PM)


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OfflineOra
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Re: Recognizing and dealing with contamination [Re: Nekonation]
    #27481870 - 09/25/21 04:12 PM (2 months, 3 days ago)

maybe they need a little more FAE? they look very lanky (unless theyre supposed to look like that) and that one in the back in the last pic has fuzz all the way up the stem.


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OfflineSpaceBabyS
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Re: Recognizing and dealing with contamination [Re: Ora]
    #27482427 - 09/26/21 07:04 AM (2 months, 2 days ago)

Quote:

Ora said:
maybe they need a little more FAE? they look very lanky (unless theyre supposed to look like that) and that one in the back in the last pic has fuzz all the way up the stem.





:whathesaid:


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

Cult Queen:

Another Day's Work:


MUSHBOY'S SHROOM TEA TEK


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OfflineNekonation
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Registered: 02/22/21
Posts: 33
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Re: Recognizing and dealing with contamination [Re: SpaceBaby]
    #27482700 - 09/26/21 02:01 PM (2 months, 2 days ago)

My only concern about FAE is the humidity getting too low for fruiting.


--------------------
:mushroom2: Why bitch about things? If you can't change them there's no point. If you can change them then bitching just wastes time you could use for making changes. :grin:


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OfflineRiv3r
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Registered: 09/26/21
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Re: Recognizing and dealing with contamination [Re: Nekonation]
    #27482776 - 09/26/21 02:57 PM (2 months, 2 days ago)

:thumbup:


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OfflineYoungshroomdude
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27482970 - 09/26/21 06:21 PM (2 months, 2 days ago)

This was a great help thank you! I have an agar plate that I put a piece of wild turkey tail on from the inside of the mushroom to avoid contamination.  And after two weeks I am starting to see a white slimy texture grow from it. Doesn’t match any of the contaminates posted above but it doesn’t really seem like mycelium. Can someone help me figure out if I have turkey tail growing or some weird contaminate?


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