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drspastic
the good doctor



Registered: 06/26/06
Posts: 147
Loc: bulgaria
Last seen: 1 year, 17 days
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#26171753 - 09/06/19 01:08 AM (4 years, 4 months ago) |
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hi, latest batch of substrate has that sour smell soon after i spawned it. corn spawn very good and clean, straw boiled for over an hour. only difference from last set of monos was i tried packing the layers of spawn and straw into clear plastic sacks stood in a empty mono to make large bricks then putting the bags on shelving.
I have read that gas exchange problems can cause smells but these are sour.
is there any cure or shall I dinv it outdoor to make another garden patch?
bricks are about 5kg each and are heavily spawned
-------------------- did you eat all that acid? you better pray to god there's some thorazine in that bag.
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Motomushy
Stranger

Registered: 09/16/19
Posts: 3
Last seen: 4 years, 4 months
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Re: Recognizing and dealing with contamination [Re: drspastic]
#26191576 - 09/16/19 11:56 AM (4 years, 4 months ago) |
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Can anyone tell me if this is contaminated? The get lighter in color as they grow
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Recognizing and dealing with contamination [Re: Motomushy]
#26192379 - 09/16/19 05:11 PM (4 years, 4 months ago) |
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Quote:
Motomushy said: Can anyone tell me if this is contaminated? The get lighter in color as they grow

Those are too dry. You need another layer of mp tape over the bottom holes. No contams that I can see.
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Motomushy
Stranger

Registered: 09/16/19
Posts: 3
Last seen: 4 years, 4 months
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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
#26192930 - 09/16/19 10:43 PM (4 years, 4 months ago) |
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Thank you! I sprayed a lil water mist in the tub and they have a blueish hue now... That's normal right?
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myc.kind.o.lady


Registered: 09/17/19
Posts: 4
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#26208652 - 09/25/19 01:31 PM (4 years, 4 months ago) |
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Hello all :-) Amazing original post, thanks for all the great info!! MUSH APPRECIATED!!
This is my first time posting anything so I'm apologizing in advance if not proper...
I've been having this issue and i'm losing sleep ...
colonized jars look and smell great - when they are spawned to bulk and after consolidation this is what is happening around day 7-10 after fruiting:


(I could try taking better pics if necessary)
My worst fear is that this is ASPERGILLUS - Can anyone confirm what it is?
**I've been retracing steps and thinking that my pasteurization technique is prob faulty - vac bags filled with bulk sub and cooked in water (170 for 2hrs) i deff over filled and over wet bulk probably causing to get too hot on outside and not enough in center ?? *** I've also had the window open (no AC and super hot in here) could this have been a factor?
if not aspergillus ...Could these blocks be saved? Maybe soaking in mild bleach solution??
I tossed out about 10 (2L) blocks last time as soon as I saw yellow (they were just starting to fruit)....so sad ... but noticed that it only affected the top - under liner everything looked and smelled healthy....
now I have 8 new tubs that are affected and hoping I can save if possible
If not able to save - no problem - health and safety first and also the MAIN thing I need to know is What am I doing wrong and how can I avoid this in the future???
Thanks so much for your time
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flyingcap
Explorer

Registered: 10/08/19
Posts: 8
Last seen: 1 month, 20 days
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Re: Recognizing and dealing with contamination [Re: myc.kind.o.lady]
#26243011 - 10/10/19 03:30 PM (4 years, 3 months ago) |
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Got a few ugly Bettys growing. Reading through some prior threads it can be over misting and lack of FAE or a genetic mutation. Can anyone advise the exact cause and solution for future growing knowledge ? Appreciate the knowledge folks.
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tomcards



Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 10 months, 19 days
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Re: Recognizing and dealing with contamination [Re: flyingcap]
#26289644 - 10/31/19 11:45 AM (4 years, 2 months ago) |
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Thanks to Kizzle for this fantastic, informative thread.
Can anyone identify the beast or beasties that contaminated this tub?
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solarflash
Stranger
Registered: 11/27/19
Posts: 1
Last seen: 3 years, 7 months
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Re: [STICKY] Recognizing and dealing with contamination [Re: Kizzle]
#26349508 - 11/27/19 08:44 PM (4 years, 2 months ago) |
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The first 4 times I ever tried the pftek method I was successful. I enjoyed the fruits of my labor. The next 6 times all ended in fast spreading green contamination. The cakes were fully colonized and put into sanitized fruiting chamber. I fanned them daily and used the same care as the first 4 successful batches. Soon after the contamination overtook all the cakes. Batch after batch until I wasted 6 spore syringes. I gave up for a couple of years but am going to try again. This time using a sterilized rye grain kit. Many posters here are very adamant about not using a kit but how bad can these kits be. What’s worse? Spending $50 on a sterilized kit or wasting syringe after syringe and not knowing exactly where you went wrong? Appreciate any constructive advice.
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ZombiWurm
Stranger

Registered: 10/15/18
Posts: 644
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Re: Recognizing and dealing with contamination [Re: tomcards]
#26366470 - 12/07/19 08:44 AM (4 years, 1 month ago) |
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Cobweb in the bottom left corner?
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drewman522
Stranger

Registered: 12/08/19
Posts: 3
Last seen: 2 years, 11 months
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Re: Recognizing and dealing with contamination [Re: ZombiWurm]
#26368810 - 12/08/19 10:48 AM (4 years, 1 month ago) |
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Thank you for this detailed write up! I'm brand new to this forum, apologies if this reply breaks any rules. And sorry to ask what may be the obvious but hoping someone more experienced than me can confirm that this cake is contaminated and should just be trashed? OR is it worth continuing?
This is our first grow and we've been following the PF Tek Easy BRF Cakes found here: https://www.shroomology.org/forums/topic/13173-pf-tek-easy-brf-cakes-mushroom-growing-method/
Started on 11/3 with 8 cakes and already had to dispose of 6 of them because of visible contams and really no white mycelium.
Any guidance is greatly appreciated!
https://imgur.com/bP32ldr
Edited by drewman522 (12/08/19 10:50 AM)
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ZombiWurm
Stranger

Registered: 10/15/18
Posts: 644
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Re: Recognizing and dealing with contamination [Re: drewman522]
#26368828 - 12/08/19 11:00 AM (4 years, 1 month ago) |
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That's the wrong jar for BRF cakes. You won't ever get the cake out of that jar. Anyway it looks fine to me. Stick to wide mouth half pint jars. I've always used the regular mouth half pints but it seems the short ones colonize faster
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Psilotyl
נָזִיר


Registered: 08/30/19
Posts: 469
Loc: עולם
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Re: Recognizing and dealing with contamination [Re: tomcards]
#26368935 - 12/08/19 12:05 PM (4 years, 1 month ago) |
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Quote:
tomcards said: Thanks to Kizzle for this fantastic, informative thread.
Can anyone identify the beast or beasties that contaminated this tub?

hard too really see there. Even so...who knows :/ I don’t think it’s penicillium. It could be aspergillus, chaetomium, trichoderma, maybe cladosporium...but clad is usually darker looking than what’s in your pic. Sadly there are so many “greens” that can eff shit up it’s hard for most of us to say what’s what with certainty
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שלום וְאור | PEACE & LIGHT
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ZombiWurm
Stranger

Registered: 10/15/18
Posts: 644
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Re: Recognizing and dealing with contamination [Re: Psilotyl]
#26369350 - 12/08/19 04:02 PM (4 years, 1 month ago) |
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Quote:
Psilotyl said:
Quote:
tomcards said: Thanks to Kizzle for this fantastic, informative thread.
Can anyone identify the beast or beasties that contaminated this tub?

hard too really see there. Even so...who knows :/ I don’t think it’s penicillium. It could be aspergillus, chaetomium, trichoderma, maybe cladosporium...but clad is usually darker looking than what’s in your pic. Sadly there are so many “greens” that can eff shit up it’s hard for most of us to say what’s what with certainty
Yeah it's really hard to tell. You could culture it but even then without a scope you are stuck with observing how it colonized the agar to determine what it is and how the mycelium grows.
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tomcards



Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 10 months, 19 days
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Re: Recognizing and dealing with contamination [Re: ZombiWurm]
#26370574 - 12/09/19 09:50 AM (4 years, 1 month ago) |
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Thanks for the feedback.
My sterile technique was sorely lacking. There were probably all manner of nasty beasties in the substrate. After one flush, I harvested 83.3 cracker-dry grams of Golden Teacher. I dumped the rest of the substrate. I kick myself as I fantasize about how much that tub could have yielded.
Since then, I've learned much. This won't happen again.
Tom
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drewman522
Stranger

Registered: 12/08/19
Posts: 3
Last seen: 2 years, 11 months
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Re: Recognizing and dealing with contamination [Re: ZombiWurm]
#26370733 - 12/09/19 11:19 AM (4 years, 1 month ago) |
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Realized I replied to the wrong post, but thanks for the reply! Wow so you think no concerns with contams based on those pics?
Thanks for the note on the jars - these are wide mouth as directed but realized too late exactly what you're saying... may end up having to break them to get the cakes out.
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Psilotyl
נָזִיר


Registered: 08/30/19
Posts: 469
Loc: עולם
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Re: Recognizing and dealing with contamination [Re: tomcards]
#26370877 - 12/09/19 12:49 PM (4 years, 1 month ago) |
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Quote:
tomcards said: Thanks for the feedback.
My sterile technique was sorely lacking. There were probably all manner of nasty beasties in the substrate. After one flush, I harvested 83.3 cracker-dry grams of Golden Teacher. I dumped the rest of the substrate. I kick myself as I fantasize about how much that tub could have yielded.
Since then, I've learned much. This won't happen again.
Tom
That’s still not bad! There’s always next time, as lame as that sounds lol
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שלום וְאור | PEACE & LIGHT
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A_Fun_Guy_Learns
Stranger

Registered: 03/09/19
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Last seen: 2 years, 4 months
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Re: Recognizing and dealing with contamination [Re: Psilotyl]
#26372091 - 12/09/19 11:54 PM (4 years, 1 month ago) |
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Is this the meany greeny, or just metabolites?
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Psilotyl
נָזִיר


Registered: 08/30/19
Posts: 469
Loc: עולם
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Quote:
A_Fun_Guy_Learns said: Is this the meany greeny, or just metabolites?

Looks like that’s probably fine. Keep an eye on it and see if it turns & you’ll get a definitive answer pretty quickly
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שלום וְאור | PEACE & LIGHT
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MushToLearn
Stranger

Registered: 01/06/20
Posts: 1
Last seen: 4 years, 15 days
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Re: Recognizing and dealing with contamination [Re: Psilotyl]
#26418832 - 01/06/20 07:06 AM (4 years, 23 days ago) |
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Hi folks, newbie here. I've just managed my first grow of 5 BRF cakes. I've done one flush so far and have dunked and setup for flush No.2. Past day or so i've noticed a very slight musky smell coming from my FC which made me head here to try get some info on possible contams. After reading a lil i am leaning towards a possible Trich contam but im not sure as i obviously lack the experience. I can still smell the Mycelium as on the first flush but now with a very slight musky smell. I don't see any obvious mold spots but after reading about the white growth that comes before Trich spores i have a feeling this is what i am faced with. If it wasn't for the slight smell i would have thought these cakes were fine. Posted some pics so if anyone would like to help out a newbie i'd appreciate the advice. Cheers.




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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,854
Last seen: 9 hours, 32 minutes
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Re: Recognizing and dealing with contamination [Re: MushToLearn]
#26419784 - 01/06/20 06:19 PM (4 years, 22 days ago) |
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All you can do is wait and see.
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