Home | Community | Message Board

Mushrooms.com
Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: < First | < Back | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | Next >
Offlinekidcharlemagne
Stranger
 User Gallery

Registered: 11/14/13
Posts: 242
Last seen: 4 hours, 28 minutes
Re: Recognizing and dealing with contamination [Re: JXAllen]
    #27452325 - 09/01/21 03:34 PM (1 month, 13 days ago)



Hey all,  this was from a likely bacterial LC that I put on carbon and  gentamicin agar (though the gentamicin was a bit old). Is the starburst pattern (weak then stronger) a result of it out running contamination, or possibly adapting to a new medium, or both? Thanks!

Edit: there are two pieces because it broke, not for any dumber reason.


Edited by kidcharlemagne (09/01/21 03:42 PM)


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 796
Last seen: 3 hours, 6 seconds
Re: Recognizing and dealing with contamination [Re: kidcharlemagne]
    #27452909 - 09/02/21 01:22 AM (1 month, 12 days ago)

I can't say for sure, but I saw the same growth pattern from some of the agar wedges I put to slants. It could be adjustment to a new medium. And next time, you can try to spread a drop of LC around more so bacteria would be more obvious than the mycelia fragments.

The point of testing LC is to see whether it has bacteria. Since you used antibacterial agar, you learned nothing from the test. Do it again on plain agar. Does the charcoal hamper bacteria? If so, skip that as well.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinekidcharlemagne
Stranger
 User Gallery

Registered: 11/14/13
Posts: 242
Last seen: 4 hours, 28 minutes
Re: Recognizing and dealing with contamination [Re: gone-pear-shaped]
    #27452947 - 09/02/21 01:50 AM (1 month, 12 days ago)

Quote:

gone-pear-shaped said:
I can't say for sure, but I saw the same growth pattern from some of the agar wedges I put to slants. It could be adjustment to a new medium. And next time, you can try to spread a drop of LC around more so bacteria would be more obvious than the mycelia fragments.

The point of testing LC is to see whether it has bacteria. Since you used antibacterial agar, you learned nothing from the test. Do it again on plain agar. Does the charcoal hamper bacteria? If so, skip that as well.




It wasn't a test, it was to clean it up because I thought it was bacterial, but I needed the strain.  I received it from someone.


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleSmartattackS
C'mon man
 User Gallery


Registered: 12/21/18
Posts: 3,244
Loc: A thought
Re: Recognizing and dealing with contamination [Re: kidcharlemagne]
    #27453161 - 09/02/21 06:53 AM (1 month, 12 days ago)

Regardless of the origin you should make transfers until cultures are clean. But I agree on skipping the amendments.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineShadow Shrooms
Stranger

Registered: 08/19/21
Posts: 7
Last seen: 14 hours, 24 minutes
Re: Recognizing and dealing with contamination [Re: Smartattack]
    #27453507 - 09/02/21 01:26 PM (1 month, 12 days ago)

Hi I'm new and just transferred my first substrate and rye grain mix into the fruiting chamber and fear for lack of experience and greater knowledge that I may have screwed the pooch on my little golden teacher cubes



Is this the start of penicillium? I fear I may have not done the transfer properly and caused the introduction of this new fungal agent into my environment

Can anyone help verify this for me?


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlineshr00mFer
Stranger
Registered: 07/07/20
Posts: 96
Last seen: 4 hours, 19 minutes
Re: Recognizing and dealing with contamination [Re: Shadow Shrooms]
    #27453554 - 09/02/21 02:17 PM (1 month, 12 days ago)

Quote:

Shadow Shrooms said:
Hi I'm new and just transferred my first substrate and rye grain mix into the fruiting chamber and fear for lack of experience and greater knowledge that I may have screwed the pooch on my little golden teacher cubes



Is this the start of penicillium? I fear I may have not done the transfer properly and caused the introduction of this new fungal agent into my environment

Can anyone help verify this for me?



Looks fine to me.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinekidcharlemagne
Stranger
 User Gallery

Registered: 11/14/13
Posts: 242
Last seen: 4 hours, 28 minutes
Re: Recognizing and dealing with contamination [Re: shr00mFer]
    #27453702 - 09/02/21 04:37 PM (1 month, 12 days ago)



Anyone know what this is?  Thanks!


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinemoshe1

Registered: 08/29/15
Posts: 346
Last seen: 5 days, 16 hours
Re: Recognizing and dealing with contamination [Re: kidcharlemagne]
    #27453725 - 09/02/21 04:53 PM (1 month, 12 days ago)

anyone have had to battle pesky trich problems in their tubs?

out of 10 or so tubs so far i've had ~3 trich out on me before F1, and 2 to trich out on me before F2.  The subs were 100% colonized for all of the tubs when trich hit.  I know i could be doing better than that. 

idk where to start besides doing a proper floor to ceiling clean up in my space. All my spawn is grown out from clean agar cultures and/or G2G'd from a master jar, which have all been looking clean and growing great so far.  The common consensus seems to be that any trich before F1 points to contaminated spawn, but keep in mind the trich is popping up like 10+ days into fruiting with fully colonized sub. I'm yet to see trich in any of my bags or jars this cycle.


--------------------


Edited by moshe1 (09/02/21 05:00 PM)


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineMycoBrainz
Mycotic
Male User Gallery


Registered: 03/14/15
Posts: 540
Loc: West Coast
Last seen: 2 days, 11 hours
Re: Recognizing and dealing with contamination [Re: moshe1]
    #27453806 - 09/02/21 05:47 PM (1 month, 12 days ago)

Quote:

moshe1 said:
anyone have had to battle pesky trich problems in their tubs?

out of 10 or so tubs so far i've had ~3 trich out on me before F1, and 2 to trich out on me before F2.  The subs were 100% colonized for all of the tubs when trich hit.  I know i could be doing better than that. 

idk where to start besides doing a proper floor to ceiling clean up in my space. All my spawn is grown out from clean agar cultures and/or G2G'd from a master jar, which have all been looking clean and growing great so far.  The common consensus seems to be that any trich before F1 points to contaminated spawn, but keep in mind the trich is popping up like 10+ days into fruiting with fully colonized sub. I'm yet to see trich in any of my bags or jars this cycle.




The tric starts white like mycelium.  It's hard to spot without lots of experience. I still get trich on my straw subs after second flush, 3rd if I'm really lucky. Just get a fruit to clone your good. Lots of clean transfers. If you think it's contam give it a week,it'll show itself.


--------------------
:stoned:


PFC x Creeper


Lets Get Stoned


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 796
Last seen: 3 hours, 6 seconds
Re: Recognizing and dealing with contamination [Re: kidcharlemagne]
    #27454318 - 09/03/21 03:00 AM (1 month, 11 days ago)

Quote:

kidcharlemagne said:


Anyone know what this is?  Thanks!



Looks like cobweb mold. Sorry for your loss. The fact that it starts from the bottom makes me think you may have inoculated with it, rather than it slipping in through the threads/filter.


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleMunchauzen
spore scammer
 User Gallery

Registered: 06/23/11
Posts: 14,190
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: moshe1]
    #27454537 - 09/03/21 09:47 AM (1 month, 11 days ago)

Quote:

moshe1 said:
anyone have had to battle pesky trich problems in their tubs?

out of 10 or so tubs so far i've had ~3 trich out on me before F1, and 2 to trich out on me before F2.  The subs were 100% colonized for all of the tubs when trich hit.  I know i could be doing better than that. 

idk where to start besides doing a proper floor to ceiling clean up in my space. All my spawn is grown out from clean agar cultures and/or G2G'd from a master jar, which have all been looking clean and growing great so far.  The common consensus seems to be that any trich before F1 points to contaminated spawn, but keep in mind the trich is popping up like 10+ days into fruiting with fully colonized sub. I'm yet to see trich in any of my bags or jars this cycle.



Possibly bacterial contamination in your spawn, which invites trich to grow at any stage. Bacteria is the shoe in the door for a lot of bad shit.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineJXAllen
Cannalyst
 User Gallery


Registered: 08/06/21
Posts: 106
Last seen: 10 hours, 12 minutes
Re: Recognizing and dealing with contamination [Re: Munchauzen]
    #27454554 - 09/03/21 09:59 AM (1 month, 11 days ago)

Did I pick up spider mold somehow?? This plate hasn't been opened since the transfer, it looked clean until today. Now It looks like the mycelium is isolating itself off and the corner is filled with cobweb.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineThe_goodfuz
Stranger
 User Gallery
Registered: 09/05/21
Posts: 2
Last seen: 1 month, 8 days
Re: Recognizing and dealing with contamination [Re: JXAllen]
    #27457304 - 09/05/21 05:28 PM (1 month, 9 days ago)

Hi so after 3 failed attempts due to obvious contam (my fault for not being sterile) I've got to a stage where I've got them into fruiting conditions. The tubs fruiting on top all the side and all over the bottom :crazy: I'm not too concerned about that but some of the fruits have this wierd splitting on them. I'm guessing it's nota good sign but just hoping one of you experienced guys can tell me if it's a write off or should I just let them grow some look perfectly normal.

https://files.shroomery.org/files/21-35/087789074-IMG_20210905_125644.jpg

https://files.shroomery.org/files/21-35/087789168-IMG_20210905_125628.jpg


Edited by The_goodfuz (09/05/21 05:49 PM)


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinegone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 796
Last seen: 3 hours, 6 seconds
Re: Recognizing and dealing with contamination [Re: The_goodfuz]
    #27457768 - 09/06/21 01:56 AM (1 month, 8 days ago)

I'm not sure what you mean by splitting, and you're not doing yourself any favors by scaling down an image that small, but normally damaged fruits is a symptom of a parasitic infection--pseudomonas or verticillium. But split caps can be due to too low humidity.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineJXAllen
Cannalyst
 User Gallery


Registered: 08/06/21
Posts: 106
Last seen: 10 hours, 12 minutes
Re: Recognizing and dealing with contamination [Re: gone-pear-shaped]
    #27457799 - 09/06/21 03:11 AM (1 month, 8 days ago)

Can I do anything about this yeast? or is this jar toast?


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineThe_goodfuz
Stranger
 User Gallery
Registered: 09/05/21
Posts: 2
Last seen: 1 month, 8 days
Re: Recognizing and dealing with contamination [Re: JXAllen]
    #27457882 - 09/06/21 06:56 AM (1 month, 8 days ago)

Sorry the pics are poor I was struggling to get them on. Its like a split straight across the stem almost like it's been cut with a knife there's 4 like that can't find any images of anything else like it. The rest all look fine though so I'll just isolate the tub and see how it goes with them.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineFeedyourhead33
Stranger
 User Gallery
Registered: 06/05/21
Posts: 18
Last seen: 4 days, 1 hour
Re: Recognizing and dealing with contamination [Re: The_goodfuz]
    #27459158 - 09/07/21 01:11 PM (1 month, 7 days ago)






Thats a contaminant right?


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineBastibla
Stranger
Registered: 01/06/21
Posts: 6
Last seen: 3 days, 9 hours
Re: Recognizing and dealing with contamination [Re: Feedyourhead33]
    #27459961 - 09/08/21 04:07 AM (1 month, 6 days ago)

Hey everyone!
I had some struggles with my autoclave and my spawn is a bit overdue.
Most of my bags for multiple strains (king oyster, oysters,  pioppino) have yellowish or orange spots. It looked fine till it was fully colonized and standing for multiple days.

I’ve started from lc -> agar and then either lc -> grain or agar -> grain.

I think my problem is that my spawn is too wet. But I’m not sure if it’s just metabolites or contamination. Really need your help:



Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineDoinker
Stranger
Registered: 09/08/21
Posts: 1
Last seen: 1 month, 6 days
Re: Recognizing and dealing with contamination [Re: Bastibla]
    #27460046 - 09/08/21 07:25 AM (1 month, 6 days ago)



Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineSpaceBabyS
alchemist
I'm a teapot User Gallery


Registered: 11/01/20
Posts: 1,074
Loc: My Own Private Idaho
Last seen: 1 hour, 9 minutes
Re: Recognizing and dealing with contamination [Re: Doinker]
    #27463180 - 09/10/21 04:35 PM (1 month, 4 days ago)

Hey hey, it's been a minute. But only cause I recognize more tams.
That said...DaFuq?

MILLET: GTc





LC: MALc



Can I get an indepth Dx?
TYIA


--------------------
SpaceBaby
SPACEBABY's LAZY FUCKER SHOEBOX LINING TEK

Cult Queen:

Another Day's Work:


Extras: Filter Print Post Remind Me! Notify Moderator Top
Jump to top Pages: < First | < Back | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | Next >

Shop: Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Contamination Identification & Subjugation NutsackRucksack 11,463 4 11/19/20 11:38 PM
by Goat 2020
* Spore Splotch? Product 741 2 08/15/01 06:44 PM
by Product
* Spread of contamination Xherrus 3,353 15 01/28/04 02:27 PM
by Anno
* Should I fruit my tub yet? Top & Sides fully Colnzed. But bottom a bit splotchy Mike Ology 686 13 08/11/12 11:21 AM
by Mike Ology
* Myco-Bag Contamination? Help Us Please! Phantastic 2,508 9 06/11/02 07:21 AM
by Phantastic
* What are these splotches? archivist 531 7 03/27/09 04:43 PM
by archivist
* Smelt Jars for contamination. I was taken to ER!!!
( 1 2 3 4 5 6 all )
JayBin 35,837 110 01/15/21 08:15 AM
by bodhisatta
* Contamination or no????
( 1 2 all )
CosmicShame 5,149 20 05/27/03 01:59 AM
by igore

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
199,348 topic views. 31 members, 164 guests and 64 web crawlers are browsing this forum.
[ Print Topic | ]
Search this thread:

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.007 seconds on 14 queries.