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OfflineLuminous7


Registered: 01/26/16
Posts: 1,110
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Re: Recognizing and dealing with contamination [Re: LadysKnight]
    #27375042 - 07/05/21 02:48 PM (3 months, 9 days ago)

:whathesaid:


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Offlinebagga
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Registered: 05/19/21
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Re: Recognizing and dealing with contamination [Re: Luminous7]
    #27375093 - 07/05/21 03:37 PM (3 months, 9 days ago)

Just finished second flush - a several pins on one end of the tub have weird orange mycelium.

It's fruited well and has so far been clean, just wondering what this is and if I should be concerned.



Another example


Thanks!


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Offlinetututotutut
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Registered: 01/18/21
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Re: Recognizing and dealing with contamination [Re: bagga]
    #27375133 - 07/05/21 04:07 PM (3 months, 9 days ago)

Hello all, pardon if not the best place to ask:
i am also having an agar problem similar to ranch's.
I have plates that have these very small hardly visible blotches on the surface away from the transfer piece/new myce growth. Its hard to get a picture of. Im under the impression it's bacteria, but i cant say if its from poor non-sterile dishes, or if its poor SAB cleanliness.
And like ranch said, are the dishes trash? Transfer away from the blotches?
It's taking me a very long time to establish +50% agar success :frown:(
Heres an attempt at a photo of suspected dish with blotches

Im hoping its just the way the agar dried... But probably bacteria right


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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,156
Re: Recognizing and dealing with contamination [Re: bagga]
    #27375366 - 07/05/21 07:20 PM (3 months, 9 days ago)

Quote:

bagga said:
Just finished second flush - a several pins on one end of the tub have weird orange mycelium.

It's fruited well and has so far been clean, just wondering what this is and if I should be concerned.



Another example


Thanks!



Very bacterial with a little mold. Surprised you got 2 flushes. Again, I put contaminated subs outside for best results.


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InvisibleLadysKnight
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Re: Recognizing and dealing with contamination [Re: tututotutut]
    #27375371 - 07/05/21 07:23 PM (3 months, 9 days ago)

Quote:

tututotutut said:
Hello all, pardon if not the best place to ask:
i am also having an agar problem similar to ranch's.
I have plates that have these very small hardly visible blotches on the surface away from the transfer piece/new myce growth. Its hard to get a picture of. Im under the impression it's bacteria, but i cant say if its from poor non-sterile dishes, or if its poor SAB cleanliness.
And like ranch said, are the dishes trash? Transfer away from the blotches?
It's taking me a very long time to establish +50% agar success :frown:(
Heres an attempt at a photo of suspected dish with blotches

Im hoping its just the way the agar dried... But probably bacteria right




I can't make out anything in the first pic, too overgrown, but the second pic does look like the outline of bacteria.

Generally, you don't want to wait this long. After you see clean growth, you want to transfer the best piece to a new plate.


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Offlinetututotutut
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Re: Recognizing and dealing with contamination [Re: LadysKnight]
    #27375480 - 07/05/21 08:37 PM (3 months, 9 days ago)

Quote:

Generally, you don't want to wait this long. After you see clean growth, you want to transfer the best piece to a new plate.



Sure, but I figured when going agar dish to grains , you want the dish to be almost fully colonized, no?


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Offlinegone-pear-shaped
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Registered: 10/30/17
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Re: Recognizing and dealing with contamination [Re: tututotutut]
    #27375738 - 07/06/21 12:41 AM (3 months, 8 days ago)

Quote:

tututotutut said:
Quote:

Generally, you don't want to wait this long. After you see clean growth, you want to transfer the best piece to a new plate.



Sure, but I figured when going agar dish to grains , you want the dish to be almost fully colonized, no?



Cleanliness is more important than the amount of mycelium transferred. If you had really good growth that didn't have potential bacteria hiding in the middle, you could grow it out and transfer the whole plate. Last time I inoculated a jar, I used a couple rice grain sized pieces from the edge because the center may have been risky. (But big wedges are more common.) Your plates don't have that lovely consistent clean look that we would want if using a whole plate for a jar. Instead, you should have transferred them.

The main issue with using a small inoculation is that it takes a few days longer, and that increases contam risk. And I've had small wedges stick to the lid and immediately stall.

What should you do now? Depends on how much materials you have, and how much you value time versus effort. Will it crush you to fail? You can spawn plate #1 and see if it gets infected. If so and if the infection is very subtle, you can spawn it to a very small tub. If it's intermediate, top-fruit it. With major infection you'll have to throw it out. But if you are stingy with your energy or materials, just make some transfers. Make the transfers even if you do choose to use the plate to inoculate, because you'll want to clean up your growth for a later stage of this project. Don't put all your eggs in one basket.


Edited by gone-pear-shaped (07/06/21 12:43 AM)


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OfflineSpaceBabyS
alchemist
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Registered: 11/01/20
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Re: Recognizing and dealing with contamination [Re: bagga] * 1
    #27375789 - 07/06/21 03:08 AM (3 months, 8 days ago)

[it would be helpfu to read how to resize a pic in the ‘start here’ section. i don’t know that much so i won’t be definitive; one of these guys will know what. but that looks nasty to me.:shrug:]

Nevermind, if i had looked i’da seen the more informative answers


Edited by SpaceBaby (07/06/21 03:17 AM)


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OfflineBrownBear
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Registered: 06/05/20
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Re: Recognizing and dealing with contamination [Re: SpaceBaby]
    #27376403 - 07/06/21 03:50 PM (3 months, 8 days ago)

I'm guessing this is mold? I have never come across this particular contaminant before. Look how the mushroom mycelium colonizes right over top of it.



Well, I was able to make transfers from the second plate. This is one plate of the 4 transfer plates that I made.



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Offlinemonkey_accident
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Registered: 05/11/19
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Re: Recognizing and dealing with contamination [Re: BrownBear]
    #27376653 - 07/06/21 07:12 PM (3 months, 8 days ago)

i like the color green on those plates what dye do u use bb?
sorry - off topic


Edited by monkey_accident (07/06/21 07:13 PM)


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OfflineBrownBear
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Re: Recognizing and dealing with contamination [Re: monkey_accident]
    #27376657 - 07/06/21 07:15 PM (3 months, 8 days ago)

I use betty crocker food coloring gel.


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Offlinesunsetrem
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Registered: 07/07/21
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Re: Recognizing and dealing with contamination [Re: BrownBear]
    #27377582 - 07/07/21 02:32 PM (3 months, 7 days ago)

Fantastic post!

I had some cobweb mold on 3 tubs. I'm going to spray with hydrogen peroxide and see if that helps control it. Unfortunately my grow conditions are far from ideal.

Thanks for the post


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OfflineSillycelium123
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Registered: 03/29/21
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Re: Recognizing and dealing with contamination [Re: sunsetrem]
    #27379019 - 07/08/21 01:13 PM (3 months, 6 days ago)

Black spot under injection site. Isolated and doesnt serm to get worse. Could I birth outside?



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OfflineB1rdMan
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Registered: 07/07/21
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27379865 - 07/08/21 10:56 PM (3 months, 5 days ago)

Hey all. Just wanted to say that was a great read. Thank you ao much for all your hard work in setting that up. I am just starting out in the world of mycology but I’m loving everything im learning so far.

I was wondering if anyone could help me confirm for me or not if my setup is contaminated. (I thought it was a monotub but i was corrected on another threat that it wasn’t. Not sure what to call it)
Anyway i wasn't able to get a concrete answer as to if this was contaminated or not. I set this box up on 6-20 and so far i only have one tiny shroom. Should i just toss this out?




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InvisibleSmartattackS
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Registered: 12/21/18
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Re: Recognizing and dealing with contamination [Re: B1rdMan]
    #27379883 - 07/08/21 11:30 PM (3 months, 5 days ago)

It's pretty shitty man 😔 Those heating mats are not helping at all.


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OfflineB1rdMan
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Registered: 07/07/21
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Re: Recognizing and dealing with contamination [Re: Smartattack]
    #27379888 - 07/08/21 11:34 PM (3 months, 5 days ago)

Ahh damn. Well its a learning experience. I actually didn’t end up using the heating mat. It was just there but never turned on. I always overbuy and end up not using stuff in the end lol. I did use the thermometer though just for monitoring and it was always around 80 degrees. I’ll need to figure out how to keep it cooler
But thanks anyway


Edited by B1rdMan (07/08/21 11:39 PM)


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Offlinetututotutut
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Re: Recognizing and dealing with contamination [Re: gone-pear-shaped]
    #27379929 - 07/09/21 12:27 AM (3 months, 5 days ago)

Gonepear dude thank you, for the past year, somewhere along the way I deluded myself into the belief that i must procure one full clean dish for one clean jar. Gospel in my head that if the myce makes it all the way 100% and stays clean in agar = golden spawn candidate. Fucking cost me hundreds omg.
Thank you. I already knew the information but yeah, right, I was way off track. Just noccd 3 jars w the trusty hole tools.


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OfflineDERRAYLD
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Re: Recognizing and dealing with contamination [Re: tututotutut] * 1
    #27380040 - 07/09/21 04:27 AM (3 months, 5 days ago)

Still can't believe how you guys butcher your sabs :tongue2:


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InvisibleLadysKnight
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Registered: 10/09/15
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Re: Recognizing and dealing with contamination [Re: DERRAYLD] * 1
    #27380182 - 07/09/21 09:46 AM (3 months, 5 days ago)

Idk, those serrated edges might keep me focused. :lol:


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Onlinemilkboy
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Registered: 05/01/21
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Re: Recognizing and dealing with contamination [Re: LadysKnight] * 1
    #27380212 - 07/09/21 10:23 AM (3 months, 5 days ago)

If it works, it works. Melted my sharp edges down with a flame. :rasta:


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