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Offlinemoshe1

Registered: 08/29/15
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Re: Recognizing and dealing with contamination [Re: JakeSully]
    #27295495 - 05/05/21 02:56 PM (8 months, 16 days ago)

i could be wrong OP, but my guess is that your agar is very rich in nutrition and the myc is growing tomentose. Prolly have a TC confirm tho


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Edited by moshe1 (05/05/21 02:56 PM)


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InvisibleSmartattackS
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Re: Recognizing and dealing with contamination [Re: moshe1] * 1
    #27295499 - 05/05/21 03:01 PM (8 months, 16 days ago)

It's possibly the reason but tomentose happens man. And it's all good.


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OfflineJakeSully
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Re: Recognizing and dealing with contamination [Re: Smartattack]
    #27295659 - 05/05/21 05:06 PM (8 months, 16 days ago)

That’s great news, thanks for the answers! :smile:


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Offlinejomanda1990
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Re: Recognizing and dealing with contamination [Re: JakeSully]
    #27296500 - 05/06/21 04:54 AM (8 months, 15 days ago)

Does this look like some type of mold to you guys? I was keeping a close look on this jar, which first developed a small spot of cubensis mycelium, and then overnight a very large area near this spot got covered with thin, less-dense and less-white-looking mycellium.



Jar was innoccd with LC made from an agar wedge.


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Edited by jomanda1990 (05/06/21 04:54 AM)


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InvisibleGoatriderS
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Re: Recognizing and dealing with contamination [Re: jomanda1990]
    #27296503 - 05/06/21 04:59 AM (8 months, 15 days ago)

Guess cobweb


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Offlinemoshe1

Registered: 08/29/15
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Re: Recognizing and dealing with contamination [Re: jomanda1990]
    #27296507 - 05/06/21 05:10 AM (8 months, 15 days ago)

a bit hard to say, could very possibly be dactylium- i'm actually having a similar problem confirming dactylium but on my trays, but has me convinced its cobb- growth looks similar to yours in the jar

you cant tell in these pictures but the casing layer i layed down is laughably thin on these trays, you could see my colonized sub through the gaps of casing all across the trays.  Thin-ass casing + spraying with peroxide just incase has made it even harder to identify for me- i'm 3 days in so far with peroxide treatment. I've had a couple other trays get cobwebb recently but they acted nothing like these are. Those trays had concentrated areas of cobwebb and the growth was so large it was impossible to miss.

These trays on the other hand have said thin growths and can be spotted all across the trays. My sense of smell isn't the greatest so its hard to get a whiff and discern it from sweet myc smell- but im sure its dactylium



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Edited by moshe1 (05/06/21 05:32 AM)


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OnlineMoopers
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27297802 - 05/07/21 12:04 AM (8 months, 15 days ago)

Help me identify this mf. Rhizopus? It appeared on two recent plates, but has appeared in the past, too. Super aerial myc.



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InvisibleAshtray161S
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Re: Recognizing and dealing with contamination [Re: Moopers]
    #27297851 - 05/07/21 12:50 AM (8 months, 15 days ago)

Quote:

Moopers said:
Help me identify this mf. Rhizopus? It appeared on two recent plates, but has appeared in the past, too. Super aerial myc.






Mayb thats whats on my plates too...
Im having a really hard time telling white mold from mycelium on my plates cuz my mycel is growing pretty whispy from spores on these plates for some reason.


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It's been a tough few months for me. Itll probably still be 1 or 2 more before I'm truly back on my feet but I'm resuming sending out prints. Please feel free to begin claiming again.
Ashtrays giveaway: https://www.shroomery.org/forums/showflat.php/Number/27379511/page/3
If I owe you spores shoot me a PM or post in the marketplace thread.
:didnazithatcoming:


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InvisibleAshtray161S
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Re: Recognizing and dealing with contamination [Re: mushboy]
    #27297854 - 05/07/21 12:53 AM (8 months, 15 days ago)

Quote:

mushboy said:
Got a regular pic? Lookin down on the culture?




I dont and I just wrapped them all up :/
I can take one when I unwrap them in 2 days to work with


--------------------
It's been a tough few months for me. Itll probably still be 1 or 2 more before I'm truly back on my feet but I'm resuming sending out prints. Please feel free to begin claiming again.
Ashtrays giveaway: https://www.shroomery.org/forums/showflat.php/Number/27379511/page/3
If I owe you spores shoot me a PM or post in the marketplace thread.
:didnazithatcoming:


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OnlineMoopers
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Re: Recognizing and dealing with contamination [Re: Ashtray161]
    #27297871 - 05/07/21 01:13 AM (8 months, 15 days ago)

Quote:

Ashtray161 said:
Quote:

Moopers said:
Help me identify this mf. Rhizopus? It appeared on two recent plates, but has appeared in the past, too. Super aerial myc.






Mayb thats whats on my plates too...
Im having a really hard time telling white mold from mycelium on my plates cuz my mycel is growing pretty whispy from spores on these plates for some reason.





Your plate myc looks a bit denser, but it's hard to see. This is really sparse and open/lacey. It's is super fast-growing. That big spot is only about 24 hours old.

Whatever this is, it walks all over the plate in 3 days or less. It ruined a lot of my tampanensis plates when I first started. It would germinate on top of the good myc or next to it and cover it before I could blink.


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OfflineInthepit
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Re: Recognizing and dealing with contamination [Re: Moopers] * 1
    #27298060 - 05/07/21 06:22 AM (8 months, 14 days ago)

"This is really sparse and open/lacy. It's is super fast-growing. That big spot is only about 24 hours old. "

Ya, I feel like this is from the work in the SAB inoculating the plate.
Look at tightening up your sterile tek. It always seems to be on the edge
and possibly the edge where you pass the blade over.

I have markers on my plates and I try to orient them so the
marker is where I pass over the plate with the scalpel, that contam usually appears there.
I'm finally getting close to <1% contam.


--------------------
GLOSSARY    :mushroom2:  :chugbeer:  :cubie:    Acronyms!


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OfflineCon
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Re: Recognizing and dealing with contamination [Re: Inthepit]
    #27300905 - 05/09/21 12:48 AM (8 months, 13 days ago)

It looked fine during grain spawn... This just looks scary now... I suck at this. Has been in the tub for... 4 or 5 days. Any suggestions for my try will be appreciated. Half tempted to see what happens after some more time anyway. I did not case it.



Edited by Con (05/09/21 12:50 AM)


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InvisibleMycelium JuiceS
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Re: Recognizing and dealing with contamination [Re: Con]
    #27300914 - 05/09/21 12:55 AM (8 months, 13 days ago)

I think deep down inside you already know the suggestion.

Welcome to Shroomery! :firecum: :mushroom2:


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OfflineCon
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Re: Recognizing and dealing with contamination [Re: Mycelium Juice]
    #27300929 - 05/09/21 01:09 AM (8 months, 13 days ago)

"throw it out and try again"


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InvisibleMycelium JuiceS
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Re: Recognizing and dealing with contamination [Re: Con]
    #27300934 - 05/09/21 01:21 AM (8 months, 13 days ago)

It looks pretty bad, and the mycelium spiking is it's way of trying to escape your contamination.  Might be best to toss.

Questions...

Did you make your own grain spawn?

What did you inoculate the grains with?  i.e. agar, spore syringe, lc


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InvisibleGoatriderS
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Re: Recognizing and dealing with contamination [Re: Mycelium Juice]
    #27300940 - 05/09/21 01:29 AM (8 months, 13 days ago)

4-5 days in?
Wasn`t expecting this from the picture.
That looks like a premix, manure based,
with a lot of exposed grains to the surface.

          :cookiemonster:


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OfflineCon
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Re: Recognizing and dealing with contamination [Re: Mycelium Juice]
    #27300941 - 05/09/21 01:29 AM (8 months, 13 days ago)

I did not purchase my own grain spawn; I purchased it off of Etsy with some really good reviews...

It's crazy though, it smelled like straight shrooms at first. Those spikes appeared when it was cultivating in the grain, so I assumed it was normal.

I used a spore print, transferred with sterilized syringe.


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OfflineCon
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Re: Recognizing and dealing with contamination [Re: Goatrider]
    #27300946 - 05/09/21 01:32 AM (8 months, 13 days ago)

Quote:

Goatrider said:
4-5 days in?
Wasn`t expecting this from the picture.
That looks like a premix, manure based,
with a lot of exposed grains to the surface.

          :cookiemonster:



Yes, it is manure based. Should I have gone with wood?
Yeah.. I was told casing wasn't necessary, so I'm sure some grain is exposed. I'm not sure what you mean by premix.


Edited by Con (05/09/21 01:35 AM)


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InvisibleGoatriderS
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Re: Recognizing and dealing with contamination [Re: Con]
    #27300950 - 05/09/21 01:39 AM (8 months, 13 days ago)

Premix = Spawn/substrate made by a stranger,
which is always an idea i don`t like.
Casing isn`t necessary, but we usually apply a thin top layer
of coco coir on top at spawning.
As you just had your mix, you couldn`t do.
Won`t say it doesn`t work without a top layer though,
but your spawn was bacterial right from the jars,
if you saw that spikey mycelium in there.

I don`t know what species you try to cultivate,
but i think it`s cubensis, therefore no woodlovers.
People just inoculate some grains, like rye, oats, wbs whatever.
Fully colonized, it gets mixed with coco coir, maybe some verm,
a thin top layer of coir on top, and that`s it :wink:

        :cookiemonster:


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OfflineCon
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Re: Recognizing and dealing with contamination [Re: Goatrider]
    #27300952 - 05/09/21 01:46 AM (8 months, 13 days ago)

Quote:

Goatrider said:
Premix = Spawn/substrate made by a stranger,
which is always an idea i don`t like.
Casing isn`t necessary, but we usually apply a thin top layer
of coco coir on top at spawning.
As you just had your mix, you couldn`t do.
Won`t say it doesn`t work without a top layer though,
but your spawn was bacterial right from the jars,
if you saw that spikey mycelium in there.

              :cookiemonster:




Thanks, I've looked forever around for that and hadn't seen anything about what it meant. I knew it wasn't normal, but that's all. I have sprayed a 1/100 bleach mixture for it (not strong enough to kill the mycelium, but strong enough to kill bacteria) in hopes of seeing some recovery. My concern was the brownish patterns, especially in the first imagine. Is this a toss due to dangerous mold condition, or is there potential to get a (albeit small) grow?


Edited by Con (05/09/21 01:48 AM)


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