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 petra24000
Stranger
Registered: 04/27/21
Posts: 4
Last seen: 2 years, 9 months
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 Re: Recognizing and dealing with contamination  [Re: Rebelutionsssss]
#27283987 - 04/27/21 02:39 PM (2 years, 11 months ago) |
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Hi,
This is my first post. Hope, I'm not doing it in a wrong place, as it turned out to be a bit hard to navigate through a forum. I'm glad to join the community, I've already gained much from the resources and from others experience.
This is my first grow, it's Pf-tek with verm. and rice, the strain is F+. The jars took more then 2 months to colonize fully and two of them never made it. I gave birth to cakes almost 3 weeks ago, they are getting indirect light from the window, I keep the temp. around 21c, keep the walls moist and giving fresh air 2 or 3 times a day. The biggest of the pins (pic.3) was developed in a jar and didn't have much of a progress since then. I gave a tin foil round shell to one of the cakes and it seems that the one is getting the most of the pins down there. As you see from the photos the things are running quite slow...
But, on top of that, I got worried about the contamination as fluffy white structure got the dark 'bluish' heads. On the last pic there's a black pin and I can't figure out if this is an abort or the contaminated one. Please, let me know your thoughts if they look contaminated and I'd appreciate your suggestions as well.
   
  
Edited by petra24000 (04/27/21 02:45 PM)
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 Munchauzen
Registered: 06/22/11
Posts: 14,344
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 Re: Recognizing and dealing with contamination [Re: petra24000]
#27284038 - 04/27/21 03:25 PM (2 years, 11 months ago) |
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Most the fruits look like they aborted. I'd pluck em.
You need to mist the cakes, not the walls. The cakes grow the mushrooms, not the walls. The cakes need the water.
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petra24000
Stranger
Registered: 04/27/21
Posts: 4
Last seen: 2 years, 9 months
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Re: Recognizing and dealing with contamination [Re: Munchauzen]
#27284084 - 04/27/21 04:31 PM (2 years, 11 months ago) |
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Thank you for a disappointment 
Everywhere I read to avoid spraying the cakes directly. Should it be done somewhere above and beyond?
I guess they got contaminated in a chamber that I keep in my bedroom (got no other place), any ideas how to reduce chances of it?
Edited by petra24000 (04/27/21 05:11 PM)
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Kaburnerz
Stranger
Registered: 07/25/20
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Re: Recognizing and dealing with contamination [Re: petra24000]
#27284464 - 04/27/21 11:02 PM (2 years, 11 months ago) |
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I've got a jar that I set aside from my current grow that thought I might try G2G with, but looking closer I see something weird. Most of the myc seems fine, but there are bits of grain that they seem to be avoiding. Are these grains contaminated with something?
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Smartattack
C'mon man
Registered: 12/21/18
Posts: 3,779
Loc: A thought
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Re: Recognizing and dealing with contamination [Re: Kaburnerz]
#27284850 - 04/28/21 08:31 AM (2 years, 11 months ago) |
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Yeah that is NOT a candidate for G2G.
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SpaceBaby
alchemist
Registered: 11/01/20
Posts: 2,030
Loc: SPACE
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: Kizzle]
#27284955 - 04/28/21 10:00 AM (2 years, 11 months ago) |
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If I had a buck for every word I've read here, I would never have to work again.
I can study all I want and gain book knowledge not experience. Some contam is easily seen, some not, at least to me.
Is this GT brf jar clean? I thought maybe cobweb top center, over the in vitro pin, while hoping clean but am in over my depth. This is GT MSS. I am only playing with this while I complete my 3rd generation A2A cleanup transfers on 6 varieties.
On the upside, this is what I am mostly getting on beloved agar:
Malabar, 5 days post transfer.
I owe the learning to you all. 
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Inthepit
Aum Mani Padme Hum
Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 2 months, 29 days
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: SpaceBaby]
#27285081 - 04/28/21 11:37 AM (2 years, 11 months ago) |
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Eww that doesn't look right. but on the bright side maybe
you haven't seen this cult with cats!
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ninja cat 09
A paranoid android
Registered: 10/11/09
Posts: 4,170
Loc: Mexico 
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: Inthepit]
#27285120 - 04/28/21 12:08 PM (2 years, 11 months ago) |
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Made some (very ugly and bad) microwave agar petris, to see how well they work. This contam is from trying to use a bougainvillea flower extract as antibiotic, which didn't work, but the other ones seem to be clean. This looks like kham yeast to me, am I right about that?
How long should I wait to make sure the other petris are clean? Just to make sure the microwave technique works as expected.
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Edited by ninja cat 09 (04/28/21 12:16 PM)
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Daddy4487
The creator
Registered: 08/18/20
Posts: 406
Last seen: 2 months, 5 days
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: ninja cat 09]
#27286086 - 04/29/21 05:16 AM (2 years, 11 months ago) |
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Hey man, this is not my first time posting to this thread but I HAD to say.... you’re dedication and advise is one of the most impressive I’ve seen... 65 pages in counting!?! Nice f*ing work brother. Here are some pics of what I think will be ready to see some substrate within 48 hours tops... lmk your opinion..
  
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Hope I can help
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Thanks for the help!
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Mycelium Juice
Here for the weed
Registered: 10/31/20
Posts: 509
Loc: planet earth
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: Daddy4487]
#27286904 - 04/29/21 05:25 PM (2 years, 11 months ago) |
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I think it's going to take longer than 48 hours unless that's LC or g2g- but even then, 48 hours would be pushing it at their current state.
The myc looks healthy, just not 48 hours ready.
Enjoy your weekend first, then go check on 'em. Don't rush up to fuck up
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Daddy4487
The creator
Registered: 08/18/20
Posts: 406
Last seen: 2 months, 5 days
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: Mycelium Juice]
#27287103 - 04/29/21 08:21 PM (2 years, 11 months ago) |
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They are g2g but definitely noted. Over eagerness is undoubtedly my biggest downfall thank you
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Hope I can help
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Thanks for the help!
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Inthepit
Aum Mani Padme Hum
Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 2 months, 29 days
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: Daddy4487]
#27287402 - 04/30/21 02:24 AM (2 years, 11 months ago) |
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Quote:
Daddy4487 said:
Over eagerness is undoubtedly my biggest downfall thank you
Hey don't think yer the only one! Something about this process really
requires patience. Jokes and memes have been made about it!
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tomcards
Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 1 year, 1 month
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: Inthepit]
#27291562 - 05/03/21 07:43 AM (2 years, 11 months ago) |
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Guys,
11 days after shaking, my rye is about 95% colonized. Today, I discovered this drop of fluid.
Is it merely a metabolite, or contamination? Please advise.
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moshe1
Registered: 08/29/15
Posts: 378
Last seen: 1 year, 9 months
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: tomcards]
#27292923 - 05/03/21 08:29 PM (2 years, 11 months ago) |
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OP, that "drop of fluid" is just mushroom fluid correct. you're good- and will probably see more of it in the future. Its good stuff.
Got a bunch of my own trays with peat / verm / perlite lime casing showing whispy growths all around. Been treating with peroxide for a couple days now but just wanted to confirm. Myc cant get through casings like this right? when i break up some chunks of casing it looks like rhizo myc growing through but i don't think thats the case.
any of this obviously look like Dactylium to you? Seems like ive been cobwebbed- just need confirmations so i could keep at it with the 3% peroxide
    
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Edited by moshe1 (05/03/21 08:30 PM)
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WyoMX
Registered: 07/06/15
Posts: 2,220
Loc: PNW
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: moshe1]
#27293055 - 05/03/21 09:22 PM (2 years, 11 months ago) |
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Quote:
moshe1 said:
OP, that "drop of fluid" is just mushroom fluid correct. you're good- and will probably see more of it in the future. Its good stuff.
While 1 drop isn't a big deal you're right about that, but seeing any metabolites isn't good stuff. Tomcards just keep an eye out and if it gets alot worse probably trash unfortunately.
As for that growth Moshe I'm not exactly sure. I know real cobweb will take a substrate over like overnight. You trying to grow pans or what? Looks really wet to me.
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moshe1
Registered: 08/29/15
Posts: 378
Last seen: 1 year, 9 months
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: WyoMX]
#27293245 - 05/04/21 01:08 AM (2 years, 11 months ago) |
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Quote:
WyoMX said:
While 1 drop isn't a big deal you're right about that, but seeing any metabolites isn't good stuff. Tomcards just keep an eye out and if it gets alot worse probably trash unfortunately.
ehh, metabolites are not a reason to worry most of the time IMO saying it'll probably end up in the trash this early is a bit dramatic, especially with bulk subs. You are right though, it can be a sign of reacting to environmental stress, it is an anti-biotic after all. If things are unusual beyond some yellow spots then you are probably in for possible contams, but still no guarantee. Dont forget Its just part of the natural mushroom life cycle as well. It is secreted in order to help break down substrate.
I meant "good stuff" because it is the anti-biotic that helps fight contams, but also a tool to help myc break down material.
Its just fresh off post spray- hit it with a bunch of peroxide and misted on top as well. I should have taken some pictures before i sprayed the growths. These few trays are prepared on the wetter side though if that changes anything.
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Edited by moshe1 (05/04/21 01:17 AM)
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tomcards
Registered: 04/24/15
Posts: 427
Loc: San Francisco
Last seen: 1 year, 1 month
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Re: Recognizing and dealing with contamination...TYIA need experienced eyes [Re: moshe1]
#27293484 - 05/04/21 07:12 AM (2 years, 11 months ago) |
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Moshe1,
Thanks for confirming that it's a metabolite. There are no other signs of contamination. I'll keep my eyes peeled.
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Ashtray161
SettledNomad
Registered: 03/21/21
Posts: 4,503
Loc: Rugby, England
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Re: Recognizing and dealing with contamination [Re: tomcards]
#27294222 - 05/04/21 03:47 PM (2 years, 11 months ago) |
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Someone on discord said this is mold and not myc, yall agree?
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mushboy 
modboy
Registered: 04/24/05
Posts: 32,778
Loc: where?
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Re: Recognizing and dealing with contamination [Re: Ashtray161]
#27294251 - 05/04/21 04:10 PM (2 years, 11 months ago) |
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Got a regular pic? Lookin down on the culture?
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JakeSully
Stranger
Registered: 05/05/21
Posts: 8
Last seen: 2 months, 12 days
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Re: Recognizing and dealing with contamination [Re: mushboy]
#27295186 - 05/05/21 07:46 AM (2 years, 11 months ago) |
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This is my first attempt.
I made Potato Dextrose Agar in shallow jars, inoculated a syringe, with spores, waited a few days for the spores to hydrate and then inoculated 2 jars.
I wonder if there is any chance that this is actually mycelium on agar? Or I grew some bacteria or something.
 
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