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InvisiblePastywhyteM
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Re: Recognizing and dealing with contamination [Re: starbones]
    #26999627 - 10/23/20 02:17 PM (1 month, 6 days ago)

Quote:

starbones said:
If after switching coir back to Ecoearth and going back to boiling doesn't stop the mold outbreaks I don't know what I'm going to do. Lost another two tubs this morning as well as two shoeboxes. All different spawn from different genetics.

Sucks, severely depressed about this. A hundred or more successful monotubs this year alone and tons of shoeboxes. No issues.




I may be wrong but have you ruled out the spawn? I don’t recall seeing if you solved the spawn issue. Cron spawned to unpasteurized hpoo and had success but only because his spawn was rock solid. If it was just some seeds or whatever in the coir I could see losing a random tub here or there but not huge fails. Anything over 20% fail makes me think it’s all spawn unless there is some random yet monumental issue at play. I doubt it’s bulk substrate though.


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Onlinestarbones
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Re: Recognizing and dealing with contamination [Re: Pastywhyte] * 1
    #26999665 - 10/23/20 02:39 PM (1 month, 6 days ago)

Yeah I went back to old grain masters from successful grows, same. Tested LC on plates it was good. Tested colonized grain on a plate it was good. Used newer spawn, some older stuff from my B+ grow. All the same.

I am going through a fungus gnat problem which is more or less coming to an end now. However as I said I had switched to beatspeat and stopped boiling in my bucket tek. Hamloaf said he had the same problem with beatspeat.
I am sure with the age of some of these spawn jars that mold would have sporulated in vitro by now.

I haven't spawned a bag ever since I had so many problems with it. This is all jars and good looking fresh mushroom smelling ones with healthy mycellium. These are recovering after their 90% shake. Was saying out of 240+ jars I've only lost 2-3% of them


Getting both trich and aspergillus so I've tossed every monotub and I'm just colonizing grow bags with spawn+sub for awhile to ensure I've nailed down the fungus gnats.


Edited by starbones (10/23/20 05:09 PM)


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Onlinestarbones
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Re: Recognizing and dealing with contamination [Re: starbones] * 1
    #26999924 - 10/23/20 05:16 PM (1 month, 5 days ago)



Little experiment from the other day.
Sterilized some distilled water in a jar and tossed in a fungus gnat in front of my flowhood. Mold likes to eat the lil fuckers so I imagine they've as people have pointed out are just covered in the shit which could be the problem.

Mold spores might not like to germinate on coir but I bet they love a gnat corpse and will germinate on it and then spread to the coir, or they find one lil bit of growing mold and track it around with em. Either way I've got a good feeling this is the vector since the sealed bags of sub+spawn I made up last week that the little shitheads couldn't get into are colonizing fine without showing contamination (yet).


Edited by starbones (10/23/20 05:17 PM)


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InvisibleF. 3
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Re: Recognizing and dealing with contamination [Re: Inthepit]
    #27000758 - 10/24/20 04:27 AM (1 month, 5 days ago)

Quote:

Inthepit said:
Quote:

I'm using an ozone generator, purge the room with that thing and trust it killed the shit I cant see.
Maybe that would help you out too.
Im using Airthereal MA10k-PRO. fairly cheap considering the trouble you are going through.




Ooooh, does it smell like just after lightning?
I had a Jacobs Ladder in my bedroom
between a window and a Maryjane plant for security!

So you don't get any lung issues with a room full of ozone?
        :goodnews:




Haha, for the greater good the manufacturer sent an instruction manual and I can search on the web how to deal with this in a safe manner.

I take out all living things out of the space I wanna ozone. not that cruel.
it would be interesting to try working with room full of ozone, once.

wait the time necessary so ozone cleared out, doesn't take much time, 30min -2h . :coffee: :spliff:
proceed.


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Onlinestarbones
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Re: Recognizing and dealing with contamination [Re: F. 3] * 1
    #27000979 - 10/24/20 10:47 AM (1 month, 5 days ago)

As of right now all the bags that I spawned two weeks ago are contamination free. Strongly believe at this point it all comes down to these fungus gnats which are being nuked between sticky strips, ACV bowls and BTI. Very relieved to see nice healthy mycelium in the bags and I'll admit there's something kinda cool about a sub/spawn brick in-a-bag.

I have one monotub that they won't seem to go near and I'm not sure why. That one is now on its second flush. It's the only monotub that survived.

Fuck fungus gnats.


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Offlinesmalltalk_canceled
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Re: Recognizing and dealing with contamination [Re: starbones]
    #27001573 - 10/24/20 07:00 PM (1 month, 4 days ago)

My azurens is bruising some
spots inside the jar.
Dryness, bacteria?



Edited by smalltalk_canceled (10/24/20 07:00 PM)


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OfflineDanMatrix
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Re: Recognizing and dealing with contamination [Re: smalltalk_canceled]
    #27015927 - 11/01/20 05:24 PM (27 days, 21 hours ago)

Was hoping someone could advise if this mycelium is contaminated with mold?  There are some green shades on the grains that make me suspicious....





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Offlinedarecar33
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Re: Recognizing and dealing with contamination [Re: DanMatrix]
    #27016701 - 11/02/20 05:24 AM (27 days, 9 hours ago)

Hello
Sommetimes im having problems with reddish contamination with red dots on top... im doing on rye grain, small jars, sterilized in pressure cooker...all the time same thing...but soometimes half of the jars are contaminated, and the other half there is nice white micelium...i have some pictures..,can somebody please halp me what i am doing wrong?? Thank you













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Offlinebunsie
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Re: Recognizing and dealing with contamination [Re: DanMatrix]
    #27016776 - 11/02/20 07:33 AM (27 days, 7 hours ago)

Quote:

DanMatrix said:
Was hoping someone could advise if this mycelium is contaminated with mold?  There are some green shades on the grains that make me suspicious....








Looks contaminated to me


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OfflineDanMatrix
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Re: Recognizing and dealing with contamination [Re: bunsie]
    #27017076 - 11/02/20 10:53 AM (27 days, 3 hours ago)

Quote:

bunsie said:
Quote:

DanMatrix said:
Was hoping someone could advise if this mycelium is contaminated with mold?  There are some green shades on the grains that make me suspicious....








Looks contaminated to me




Well that sucks.  Thank you for your feedback.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27020157 - 11/03/20 09:50 PM (25 days, 16 hours ago)

I spawned a tub of PE on Friday (10/30/20). Its been about 5 days so far and I am already at 80-90% colonization. Was wondering if I could get some feedback and opinions on whether this tub is contaminated or not. Attached are some photos of the tub. Had a weird smell coming off it when I opened it to take some pics and am starting to notice some really fuzzy mycelium around the edges of the tub. This colonization time seems extremely fast to me and I am a bit worried I might be dealing with cowed mold. Sorry about the lighting, took these pics at night and used a flashlight to shine it on the tub to try and make the mycelium a little more visible. I was also wondering what you guys think about taping up holes during colonization or stuffing with polyfil. I am starting to think my sub will not get as wet during colonization if I start stuffing the holes with polyfil when I spawn the tub instead of covering with duct tape.























Edited by bradical (11/03/20 09:54 PM)


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Offlinebunsie
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Re: Recognizing and dealing with contamination [Re: bradical]
    #27021382 - 11/04/20 02:34 PM (25 days, 9 minutes ago)

I don't see any contamination, but it could be maybe deeper in the substrate depending on the smell?


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Re: Recognizing and dealing with contamination [Re: bunsie]
    #27029148 - 11/08/20 09:24 PM (20 days, 17 hours ago)

This is my first time growing and once I broke up the mycelium when about 70 percent of the bag colonized (and was totally white), some black specks have been forming. I am wondering if this is contamination and if I should not continue to the next step. It looks like specks on the grain and I can’t tell if it’s just the grain or mold. http://


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Re: Recognizing and dealing with contamination [Re: Poosh12]
    #27030047 - 11/09/20 01:31 PM (20 days, 1 hour ago)

Hey folks... sad day. I've got my first couple of North Spore spawn bags inoculated with B+ LC. They have been populating for around 3 months-- though maybe more. Second picture is a few weeks younger than the first, and I redistributed them three times over that time period. Now they finally seem fully populated when I handle em, but boy oh boy...





Did I just wait too long? These look awfully bad... so much condensation at the bottom. I've gotta start all over with new culture, don't I? I'm heartbroken.

edit: argh, sorry the images are too big-- can't seem to resize with bbcode


Edited by Sleet (11/09/20 01:40 PM)


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Re: Recognizing and dealing with contamination [Re: Sleet]
    #27030077 - 11/09/20 01:43 PM (20 days, 1 hour ago)

Yeah, that's a toss.

you should have shaken better the whole thing after inoculation so LC would have been distributed much better through out the bag.
maybe the tek u used to inoc. wasnt executed properly.


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Re: Recognizing and dealing with contamination [Re: F. 3]
    #27030124 - 11/09/20 02:15 PM (20 days, 28 minutes ago)

A real drag. Thought I was being awfully careful, liberal isopropyl use on hands, bag, work surfaces, flame sterilized needle-- but that's troubleshooting. Not looking forward to another few months without antidepressants.

Maybe I'll try agar next... finally get that pressure cooker.


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OfflineInthepit
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Re: Recognizing and dealing with contamination [Re: Sleet] * 1
    #27030218 - 11/09/20 03:09 PM (19 days, 23 hours ago)

Quote:

Sleet said:
Hey folks... sad day
edit: argh, sorry the images are too big-- can't seem to resize with bbcode




Try this How to add a picture to a post


--------------------
GLOSSARY            :chugbeer:


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Onlinethe man
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Re: Recognizing and dealing with contamination [Re: Inthepit]
    #27030904 - 11/10/20 01:14 AM (19 days, 13 hours ago)

3 months colonizing!! something aint right sounds like got a mix in ur LC :wink:


poosh: it looks like the knots are just bruising looks blue more then black?


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OfflineGnidrolog
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27034013 - 11/11/20 08:48 PM (17 days, 17 hours ago)

Is this jar bacterial? Noc'd 1 month ago via G2G.




Edit: These are oats.


--------------------


Edited by Gnidrolog (11/11/20 08:55 PM)


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Onlinestarbones
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Re: Recognizing and dealing with contamination [Re: Gnidrolog]
    #27034777 - 11/12/20 10:29 AM (17 days, 4 hours ago)

Wait for another opinion but when I get metabolites everywhere like such and uncolonized surfaces on grains against glass like such without anything more going on it's usually bacillus. Something I only ever get with oats and poopcorn.


--------------------
Clean your home.
Secret bulk recipe, broken jar of colonized spawn submerged in half a
can of concrete sealer paint cased with coir.



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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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