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Offlinekoma23
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Registered: 09/15/20
Posts: 100
Last seen: 3 hours, 22 minutes
Re: Recognizing and dealing with contamination [Re: Tolldom]
    #26936352 - 09/15/20 04:30 AM (3 years, 6 months ago)

Hi all! So I ordered a premade box. I was lazy at growing and ran out of shrooms and needed some asap. I figured that before I created my jars, these would already be ready for the first harvest.

After like 24h, the box started showing this. Is this cobweb, did I got shipped a contaminated box, is the box a goner? Tnx < 3


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OfflineFarmerEric
Friend
Registered: 04/06/20
Posts: 46
Last seen: 3 years, 3 months
Re: Recognizing and dealing with contamination [Re: koma23]
    #26936978 - 09/15/20 12:33 PM (3 years, 6 months ago)



Total noob still learning. Is this mold or myc? Is there a guide so I can learn to ID the difference?

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OfflineAlsetAlokin
Student

Registered: 07/30/20
Posts: 182
Last seen: 3 years, 3 months
Re: Recognizing and dealing with contamination [Re: FarmerEric]
    #26937122 - 09/15/20 01:48 PM (3 years, 6 months ago)

Quote:

FarmerEric said:


Total noob still learning. Is this mold or myc? Is there a guide so I can learn to ID the difference?




Not a veteran, but looks like a lot of myc genetics which is common from multispore.

At this point it is difficult to even see contamination if it is in there. Things like bacteria like to hide.

Transfer a couple of the leading edges to new plates and observe the growth from there. You could think of it as zoomimg in. Right now you're at 10x magnification, next plate will be 100x etc...

Tldr: looks good to me. Wouldn't put it to grain yet, but you're on the right track.

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OfflineFarmerEric
Friend
Registered: 04/06/20
Posts: 46
Last seen: 3 years, 3 months
Re: Recognizing and dealing with contamination [Re: AlsetAlokin]
    #26937849 - 09/15/20 07:01 PM (3 years, 6 months ago)

Quote:

AlsetAlokin said:
Quote:

FarmerEric said:


Total noob still learning. Is this mold or myc? Is there a guide so I can learn to ID the difference?




Not a veteran, but looks like a lot of myc genetics which is common from multispore.

At this point it is difficult to even see contamination if it is in there. Things like bacteria like to hide.

Transfer a couple of the leading edges to new plates and observe the growth from there. You could think of it as zoomimg in. Right now you're at 10x magnification, next plate will be 100x etc...

Tldr: looks good to me. Wouldn't put it to grain yet, but you're on the right track.





Thank you, what do you think of this wild isolation?


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Offlineeucario
Stranger
Registered: 07/25/20
Posts: 6
Last seen: 3 years, 4 months
Re: Recognizing and dealing with contamination [Re: FarmerEric]
    #26938658 - 09/16/20 09:29 AM (3 years, 6 months ago)

Hi guys, started my 2nd culture and this time I didn't have syringes ready to inoculate so I made my own from spore prints. After 1 week I started seeing these black spots in almost every jar, is it black mold? Am I fucked and will have to throw everything away? In case it is black mold, what could have caused the contamination? I didn't flame the syringe after every inoculation (yes, absolutely stupid) so I'm afraid it could have been that (used glove box, btw).






Edited by eucario (09/16/20 09:37 AM)

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OfflineFarmerEric
Friend
Registered: 04/06/20
Posts: 46
Last seen: 3 years, 3 months
Re: Recognizing and dealing with contamination [Re: eucario]
    #26938995 - 09/16/20 12:58 PM (3 years, 6 months ago)

Quote:

eucario said:
Hi guys, started my 2nd culture and this time I didn't have syringes ready to inoculate so I made my own from spore prints. After 1 week I started seeing these black spots in almost every jar, is it black mold? Am I fucked and will have to throw everything away? In case it is black mold, what could have caused the contamination? I didn't flame the syringe after every inoculation (yes, absolutely stupid) so I'm afraid it could have been that (used glove box, btw).










Did you consider your spore print was contaminated?

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InvisibleWyoMX
I'm a teapot User Gallery
Registered: 07/06/15
Posts: 2,220
Loc: PNW
Re: Recognizing and dealing with contamination [Re: eucario]
    #26939062 - 09/16/20 01:29 PM (3 years, 6 months ago)

My first guess would be dirty syringe first then after that the glovebox. If those were my jars i would throw them out.

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Offlineeucario
Stranger
Registered: 07/25/20
Posts: 6
Last seen: 3 years, 4 months
Re: Recognizing and dealing with contamination [Re: WyoMX]
    #26939271 - 09/16/20 03:16 PM (3 years, 6 months ago)

Quote:

WyoMX said:
My first guess would be dirty syringe first then after that the glovebox. If those were my jars i would throw them out.




Yeah. Probably because I forgot to flame it after each injection, or maybe the air inside the glove box was contaminated as well.

If those spots don't grow would you throw them as well?

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Offlineeucario
Stranger
Registered: 07/25/20
Posts: 6
Last seen: 3 years, 4 months
Re: Recognizing and dealing with contamination [Re: FarmerEric]
    #26939276 - 09/16/20 03:17 PM (3 years, 6 months ago)

Quote:

FarmerEric said:
Quote:

eucario said:
Hi guys, started my 2nd culture and this time I didn't have syringes ready to inoculate so I made my own from spore prints. After 1 week I started seeing these black spots in almost every jar, is it black mold? Am I fucked and will have to throw everything away? In case it is black mold, what could have caused the contamination? I didn't flame the syringe after every inoculation (yes, absolutely stupid) so I'm afraid it could have been that (used glove box, btw).










Did you consider your spore print was contaminated?




Maybe. It would be really bad bc I have some spores left I was planning to use in case I throw these out. What is the best way to store them to avoid contam?

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OfflineNibs
Peasant
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Registered: 02/17/20
Posts: 39
Loc: 🇨🇦
Last seen: 2 years, 9 months
Re: Recognizing and dealing with contamination [Re: eucario]
    #26940063 - 09/17/20 03:28 AM (3 years, 6 months ago)

Hey homie`s, I`m having some issues identifying if this is bacteria I`m dealing with. Every one of my culture plates I got from an APE swab look like this. there is clear sectoring so im thinking its just very different mycelium than what I`m used to.


When it goes to jars though it ends up looking like this, which really convinced me its bacteria because of the uncolonized grains pressed against the glass, how thick it looks, and some of the jars i left for a while continued to fuzz up nearly to the lid and almost turn into solid bricks.



I ended up putting some in a mini mono and everything seems to be looking fine with the pins coming in, so now i have no idea if this is clean myc or not...

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InvisibleShroomluvr69
luvr of shrooms


Registered: 09/25/19
Posts: 32
Loc: The Netherlands Flag
Re: Recognizing and dealing with contamination [Re: Nibs]
    #26940100 - 09/17/20 04:48 AM (3 years, 6 months ago)

I have some odd fungus growing I think. At least, it doesn't look like the P. cubensis I know... It has weird black dots in the mycellenium/hairs.


--------------------
"When in doubt, double the dose."
Terence McKenna

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OfflineWoolyMarmot
Stranger
Registered: 06/01/20
Posts: 38
Last seen: 2 years, 8 months
Re: Recognizing and dealing with contamination [Re: Shroomluvr69]
    #26940145 - 09/17/20 05:56 AM (3 years, 6 months ago)

cobweb

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Offlinexgfnxf
Stranger
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Registered: 04/27/20
Posts: 6
Last seen: 7 months, 16 hours
Re: Recognizing and dealing with contamination [Re: WoolyMarmot]
    #26943241 - 09/18/20 10:12 PM (3 years, 6 months ago)

I accidentally unloaded some contaminated spawn into my monotub substrate. it smelt and looked green.  I tried to remove it but it spread throughout the substrate.  Stemmed to slow down was pinning started.  Kind like thin licen. Thoughts?
https://files.shroomery.org/files/20-38/048862518-IMG_20200916_165643_916.jpg

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OfflineCrashTest
Stay Learning


Registered: 06/21/20
Posts: 623
Last seen: 9 months, 15 days
Re: Recognizing and dealing with contamination [Re: Shroomluvr69]
    #26943537 - 09/19/20 04:52 AM (3 years, 6 months ago)

Quote:

Shroomluvr69 said:
I have some odd fungus growing I think. At least, it doesn't look like the P. cubensis I know... It has weird black dots in the mycellenium/hairs.





That’s mold. Don’t mist it or open that tub inside.


--------------------
:mushroom2: this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020:mushroom2:


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OfflineInthepit
Aum Mani Padme Hum
Male User Gallery


Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 2 months, 29 days
Re: Recognizing and dealing with contamination [Re: xgfnxf]
    #26943549 - 09/19/20 05:06 AM (3 years, 6 months ago)

Quote:

xgfnxf said:
I accidentally unloaded some contaminated spawn into my monotub substrate. it smelt and looked green.  I tried to remove it but it spread throughout the substrate.  Stemmed to slow down was pinning started.  Kind like thin licen. Thoughts?
https://files.shroomery.org/files/20-38/048862518-IMG_20200916_165643_916.jpg




               


--------------------
:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

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Offlinearrows240
Stranger

Registered: 09/23/20
Posts: 1
Last seen: 3 years, 6 months
Re: Recognizing and dealing with contamination [Re: Inthepit]
    #26951322 - 09/23/20 03:34 PM (3 years, 6 months ago)

Hi. I hope i'm in the right place? I'm having a heck of a time navigating Shroomery.

I have a contamination problem in my outdoor azurescens bed. I got a good batch of shrooms last year and I thought I had removed most of the contaminate. However i'm now seeing that the contaminate did indeed make a vengeful come back this year.

Do I let the bed go? (I was lucky to find mycilium on some sticks a few years ago but maybe i can get some more, off the sand dunes somehow?)

Or, Do I try to rescue the couple of little islands i see that are still looking like they are solid azurescens mycilium? (this is what i'm leaning towards)

Thanks in advance.

Sorry if i misposted or said something i wasn't supposed to. I read a lot of confusing rules and tried to keep it PC.

Thanks again. Happy munching!

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OfflineEloquentParadox
Stranger
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Registered: 09/07/20
Posts: 22
Last seen: 3 years, 3 months
Re: Recognizing and dealing with contamination [Re: Kizzle]
    #26957439 - 09/27/20 01:05 PM (3 years, 6 months ago)

First attempt.. contamination?




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OfflineHikeadellic
Fungi Fan
Male


Registered: 08/31/20
Posts: 1,227
Loc: Appalachian Trail
Last seen: 23 days, 16 hours
Re: Recognizing and dealing with contamination [Re: EloquentParadox]
    #26959388 - 09/28/20 08:05 PM (3 years, 6 months ago)

Might be tamed bro :frown:

Look on the Brightside! You can try to save your grow by throwing the mycelium into a flowerpot with around 1:1 with potting soil. Sure it will probably contam too but you still have a decent chance at getting some fruit!

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OfflineEloquentParadox
Stranger
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Registered: 09/07/20
Posts: 22
Last seen: 3 years, 3 months
Re: Recognizing and dealing with contamination [Re: Hikeadellic]
    #26959694 - 09/29/20 03:53 AM (3 years, 6 months ago)

Is it safe to open a contanimated jar though? I always thought you were supposed to put them into the bin?

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OfflineRaiko112
Down under
Male User Gallery

Registered: 08/23/20
Posts: 4
Loc: Australia
Last seen: 2 years, 6 months
Re: Recognizing and dealing with contamination [Re: EloquentParadox]
    #26959756 - 09/29/20 05:56 AM (3 years, 6 months ago)

Hi you all!
Very new to growing. Failed my first brf cakes, but giving it another go with mazatapec cakes. The have been colonising for 2 weeks and seemed to be ready to go. So opened one of them up and there's lot a fluffy mycelium. Are they any good?

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Myyco.com APE Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates   OlympusMyco.com Sterilized Grain Bag   North Spore Bulk Substrate


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