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InvisiblePastywhyteM
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Re: Recognizing and dealing with contamination [Re: psychedethiogenic]
    #23893565 - 12/04/16 01:43 PM (2 years, 3 months ago)

Hard to say. If it's in every jar it's probably a bad syringe or bad inoculation procedure.


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Offlinekrudam2
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Re: Recognizing and dealing with contamination [Re: Pastywhyte]
    #23939886 - 12/19/16 10:51 AM (2 years, 2 months ago)

thanks for the post.

could we get a picture of metabolites?

I don't know the difference between normal metabolites and infection, and I've had a commercial oyster bag (bought at store) have a large orange bit but customer service told me it was fine and it seemed to grow fine


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OfflineThe Mycologist
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Re: Recognizing and dealing with contamination [Re: psychedethiogenic]
    #23951013 - 12/23/16 10:02 AM (2 years, 2 months ago)

Well whats the deal with that lid?


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OfflineKizzle
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Re: Recognizing and dealing with contamination [Re: krudam2]
    #23984419 - 01/05/17 06:31 PM (2 years, 2 months ago)

Quote:

krudam2 said:
thanks for the post.

could we get a picture of metabolites?

I don't know the difference between normal metabolites and infection, and I've had a commercial oyster bag (bought at store) have a large orange bit but customer service told me it was fine and it seemed to grow fine



I added some. Metabolites normally appear in very amounts as in the pics but if the substrate has fully colonized for quite a while or it's been sitting in particularly warm conditions you might see more. If you're seeing large puddles or uncolonized areas then it could still be metabolites your seeing but probably the result of a contaminant.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #23987543 - 01/06/17 09:26 PM (2 years, 2 months ago)

Excellent article. I always send people the link to this thread when they ask questions about contamn.


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OfflineJennifer1
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #23998459 - 01/10/17 04:53 PM (2 years, 2 months ago)

Smells like coconut! Thank you


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InvisibleMushenstein
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Re: Recognizing and dealing with contamination [Re: Jennifer1]
    #24063715 - 02/03/17 11:22 PM (2 years, 1 month ago)

Wow, That was an impressive read. Damn fine job.


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Re: Recognizing and dealing with contamination [Re: Mushenstein]
    #24198792 - 03/28/17 04:48 AM (1 year, 11 months ago)

I'm a total noob at this. This is my first attempt. I had 2 spore syringes and decided to inoculate 3 cakes and jar of liquid per syringe. I sterilized a small room, enclosed it with plastic sheeting, spritzed everything with a bleach solution, used a sterilized glove box, wore a tyvex suit, gloves, a respirator, flame sterilized the needle with an alcohol burner, sterilized the cakes in a pressure cooker @15 p.s.i. for 20 min. and the liquid for 15 to avoid caramalezation and after inoculation everything has been between 70-80 F. Mycelium growth in all six cakes appears fine after 4 days. One of the liquid cultures appears to have no activity while the other has this puffy ball with black spores from the syringe on it. Is this F.U.B.A.R.? Should I inoculate one new cake and see what happens, or should I just pitch it? Thanks.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #24203225 - 03/29/17 07:07 PM (1 year, 11 months ago)

I was also hoping to identify this "stuff" in my one jar... Not to hopeful about it :-(




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Re: Recognizing and dealing with contamination [Re: Aliceinwonderland3]
    #24203243 - 03/29/17 07:15 PM (1 year, 11 months ago)

Quote:

Aquatic Wombat said:




Defintely pitch, try to germinate and clean on agar before trying liquids.

Quote:

Aliceinwonderland3 said:
I was also hoping to identify this "stuff" in my one jar... Not to hopeful about it :-(






Idk it down to the species or even the genus, but it's definitely not clean. Looks like aspergillus maybe.


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #24203642 - 03/29/17 09:48 PM (1 year, 11 months ago)

My first Pasty Plate agar try.  I'm guessing this is Penicillium?  It's the only one so far so I'm hoping the others are viable.


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Offlinedart21
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Re: Recognizing and dealing with contamination [Re: Zachorion]
    #24225537 - 04/07/17 02:55 PM (1 year, 11 months ago)

:thumbup: Probs Zach, but cannot be certain from that photo.


Edited by dart21 (04/07/17 02:57 PM)


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Re: Recognizing and dealing with contamination [Re: dart21]
    #24233674 - 04/11/17 01:05 AM (1 year, 11 months ago)



1. Should I be concerned or worried about the cloudier, more solid area in the middle of the plate? I always notice my initial transfer wedge, being higher up than the level plate surface, bubbles up cloudy-like until it flattens out on the receiving plate's horizon. Is that what I'm seeing here?

2. Should the big divide between 11 and 12 concern me?


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OfflineDlurch314
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #24234306 - 04/11/17 11:06 AM (1 year, 11 months ago)

Hey guys in not sure if this is bruising or some kind of contam any ideas?



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Re: Recognizing and dealing with contamination [Re: Dlurch314]
    #24237185 - 04/12/17 12:42 PM (1 year, 11 months ago)

Bruising because its hella contaminated with bacteria


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Re: Recognizing and dealing with contamination [Re: Mad Season]
    #24237454 - 04/12/17 02:57 PM (1 year, 11 months ago)

So what would you do in my case? Would you pitch it or give it a shot?


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Re: Recognizing and dealing with contamination [Re: Dlurch314]
    #24237630 - 04/12/17 04:05 PM (1 year, 11 months ago)

lol, absolutely get rid of it. Give what a shot? Releasing bacteria into your living area? That's not a shot; it's a guarantee.


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OfflineThe Mycologist
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Re: Recognizing and dealing with contamination [Re: stareatclouds]
    #24237645 - 04/12/17 04:11 PM (1 year, 11 months ago)

Yea i dont like the look of that, transfer away from the cloudiness


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Re: Recognizing and dealing with contamination [Re: stareatclouds]
    #24241042 - 04/13/17 07:17 PM (1 year, 11 months ago)

Already in the dumpster thanks man


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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #24470851 - 07/09/17 08:10 PM (1 year, 8 months ago)

Good info, thanks.

After reading this thread I thought these were contaminated, need a second opinion.

They are Pan. Cyan Cambo Sandose.  This is my first grow.
Noc on 5-17, I had two jars start colonizing almost a month after inoc. I've had the rest of the jars sitting in a dark closet waiting for the last two to get to 100%. The closet averages 77-80F, 40-46% humidity.
I've been told the yellow circle is a sclerotia. I hope it is, that would be a cool surprise.

I'm growing these because they're f*cking awesome! A friends friend grew them some time ago and I was hooked. So now I'm trying to grow them. I had my friend look at them and he told me he thought he remembered bluing when his friend grew them.

What are your opinions?  Good or toss?
Anything I can do to save them? Let them sit under a UVC light if needed?

If they're f*cked, is it because I let them sit too long?

I read this in another thread, "Dip them into a sol'n of %10 H2O2, drain them, and return them to their jars."  Is this an option?





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Edited by outofservice (07/10/17 09:56 PM)


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