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OfflineMycoSnailCarl
Stranger

Registered: 11/22/23
Posts: 181
Last seen: 1 day, 10 hours
Re: Recognizing and dealing with contamination [Re: RockinRobot]
    #28593883 - 12/22/23 10:02 AM (1 month, 5 days ago)

Beautiful.


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OfflinePresidente
Stranger

Registered: 11/15/23
Posts: 21
Last seen: 7 days, 7 hours
Re: Recognizing and dealing with contamination [Re: MycoSnailCarl]
    #28594276 - 12/22/23 04:32 PM (1 month, 5 days ago)

where should I take transfers from these two contaminated plates?




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OfflinePresidente
Stranger

Registered: 11/15/23
Posts: 21
Last seen: 7 days, 7 hours
Re: Recognizing and dealing with contamination [Re: Presidente]
    #28594321 - 12/22/23 05:08 PM (1 month, 5 days ago)

Premade agar plates looks like they are contaminated?



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InvisibleFloret
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Registered: 01/02/23
Posts: 260
Loc: underneath the water
Re: Recognizing and dealing with contamination [Re: Presidente]
    #28594672 - 12/23/23 01:18 AM (1 month, 5 days ago)

I would try 4-6 on the first plate with at least another transfer after that.

The premade plate is definitely contaminated.


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LAGM 2024


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OfflineDRAKENCL
Stranger
Registered: 02/02/23
Posts: 6
Last seen: 7 days, 16 hours
Re: Recognizing and dealing with contamination [Re: Floret]
    #28597103 - 12/24/23 09:11 PM (1 month, 3 days ago)

Hello, I have a question about my last boxes, the growth stagnates and they look like rot, I would like to know your opinions


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Edited by DRAKENCL (12/24/23 09:13 PM)


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OfflineSmellyhobbit
Actual Retard
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Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 24 minutes, 14 seconds
Re: Recognizing and dealing with contamination [Re: DRAKENCL]
    #28597106 - 12/24/23 09:13 PM (1 month, 3 days ago)

Super dry. You need to initiate heavy misting until it stops drinking, then maintain surface conditions. Reduce FAE if possible. Maybe even bottom water this. She THIRSTY


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A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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OfflineDRAKENCL
Stranger
Registered: 02/02/23
Posts: 6
Last seen: 7 days, 16 hours
Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #28597308 - 12/25/23 04:53 AM (1 month, 3 days ago)

Thanks for your help, repeat what you said.
Now I have another question: should I use a hairdressing-type sprayer or a plant sprayer? I use the latter. Could it be that it hurts the buckets?
Finally I show you a photo of the growth that I am trying to describe to you.


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OfflineDRAKENCL
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Registered: 02/02/23
Posts: 6
Last seen: 7 days, 16 hours
Re: Recognizing and dealing with contamination [Re: DRAKENCL]
    #28597309 - 12/25/23 04:55 AM (1 month, 3 days ago)

For humidity I use this tool, is it wrong?


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InvisibleGoatrider
Rhythm Guitarist
Male


Registered: 04/08/20
Posts: 4,396
Loc: Germany
Re: Recognizing and dealing with contamination [Re: DRAKENCL]
    #28597449 - 12/25/23 10:16 AM (1 month, 2 days ago)

You want fine mist for not hurting mycelium,
and to leave the fine water pearls intact
while landing on the surface.
Hairdesser pumps are the best imo,
but if you know what you´re doing you can use else.

          :cookiemonster:


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OfflineDRAKENCL
Stranger
Registered: 02/02/23
Posts: 6
Last seen: 7 days, 16 hours
Re: Recognizing and dealing with contamination [Re: Goatrider]
    #28597469 - 12/25/23 10:55 AM (1 month, 2 days ago)

Thank you very much for your opinion, I share it.


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InvisibleGoatrider
Rhythm Guitarist
Male


Registered: 04/08/20
Posts: 4,396
Loc: Germany
Re: Recognizing and dealing with contamination [Re: DRAKENCL] * 1
    #28597473 - 12/25/23 11:05 AM (1 month, 2 days ago)

Forgot to mention to keep distance to the surface.
A fine mister will also hurt myc when applied straight.
Always mist from high above and let them pearls land smoothly.

              :cookiemonster:


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Offlinefnulnu
Student

Registered: 12/20/23
Posts: 15
Last seen: 1 day, 18 hours
Re: Recognizing and dealing with contamination [Re: Goatrider]
    #28610182 - 01/05/24 06:54 AM (23 days, 1 hour ago)



Hey now
Made some agar plates with mea purchased online, inoculated a couple dozen, and the Ps cyanescens is doing that blue stuff, not sure what it is, as i thought trich was green, and that shit looks blue.
Sorry bout the pic, brought the plates with me...
What is it what caused it and where can I read more about stopping it.
All other plates seem fine, but cyans/azis are what I want to innocuoate the world with
Thank you


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OnlineSupaThaRipper
Genetics Hoarder
I'm a teapot User Gallery


Registered: 09/02/13
Posts: 1,491
Loc: USA
Last seen: 1 second
Re: Recognizing and dealing with contamination [Re: fnulnu]
    #28610199 - 01/05/24 07:29 AM (23 days, 28 minutes ago)

Could be bruising due to stress. Could be bacteria under that or it’s just flat out mold. Do you have a better plate to work with


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OfflineGenome794
Spore-Whore-der & Agar-Addict


Registered: 07/20/22
Posts: 437
Loc: The Blue Planet
Last seen: 5 hours, 46 minutes
Re: Recognizing and dealing with contamination [Re: fnulnu] * 2
    #28610272 - 01/05/24 09:11 AM (22 days, 22 hours ago)

Looks green to me. Could be the white balance of the pic.

You say you noc'd a dozen or so... If it were me, that plate would be dumped. I have never had anything good from a plate that looked like that.

Sorry

794


--------------------
Looking at myc on agar never gets old.

LAGM-2023
Bigfootscreepyuncl - LAGM2023 - Challenge


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InvisibleGoatrider
Rhythm Guitarist
Male


Registered: 04/08/20
Posts: 4,396
Loc: Germany
Re: Recognizing and dealing with contamination [Re: fnulnu] * 3
    #28610289 - 01/05/24 09:41 AM (22 days, 22 hours ago)

Quote:

fnulnu said:


Hey now
Made some agar plates with mea purchased online, inoculated a couple dozen, and the Ps cyanescens is doing that blue stuff, not sure what it is, as i thought trich was green, and that shit looks blue.
Sorry bout the pic, brought the plates with me...
What is it what caused it and where can I read more about stopping it.
All other plates seem fine, but cyans/azis are what I want to innocuoate the world with
Thank you




To me that´s either penicillium or aspergillus.

            :cookiemonster:


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OfflineJW123
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Registered: 01/17/22
Posts: 1,303
Loc: Tn
Last seen: 15 hours, 8 minutes
Re: Recognizing and dealing with contamination [Re: Goatrider] * 1
    #28610563 - 01/05/24 02:04 PM (22 days, 17 hours ago)

Does anyone know what would cause mushrooms to turn into a slimey black mass in a day or two. I had to harvest my subtropicalis because whatever it is seems to be spreading. Should I worry about my other tubs?


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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,655
Re: Recognizing and dealing with contamination [Re: JW123] * 2
    #28610740 - 01/05/24 04:10 PM (22 days, 15 hours ago)

Looks wet af in there, likely bacterial, got mold creeping from the left.


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OfflineJW123
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Registered: 01/17/22
Posts: 1,303
Loc: Tn
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Re: Recognizing and dealing with contamination [Re: LadysKnight]
    #28610860 - 01/05/24 06:09 PM (22 days, 13 hours ago)

Thank you. I have been misting it daily to try to get them to produce spores, but maybe I am not giving them enough FAE.


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OfflineGastronomicus
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Registered: 03/31/05
Posts: 9,727
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Re: Recognizing and dealing with contamination [Re: JW123] * 2
    #28610870 - 01/05/24 06:19 PM (22 days, 13 hours ago)

Just don't mist. It looks like mud in there


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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OfflineJW123
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Registered: 01/17/22
Posts: 1,303
Loc: Tn
Last seen: 15 hours, 8 minutes
Re: Recognizing and dealing with contamination [Re: Gastronomicus]
    #28610877 - 01/05/24 06:28 PM (22 days, 13 hours ago)

I haven't been able to get these things to produce spores. Misting daily is the advice for this species to get them to sporulate. I have more tubs going. I threw that one out. I will mist my next run less often and try to find the sweet spot. Thank you for the response.


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