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MycoSnailCarl
Stranger

Registered: 11/22/23
Posts: 181
Last seen: 1 day, 10 hours
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Re: Recognizing and dealing with contamination [Re: RockinRobot]
#28593883 - 12/22/23 10:02 AM (1 month, 5 days ago) |
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Beautiful.
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Presidente
Stranger

Registered: 11/15/23
Posts: 21
Last seen: 7 days, 7 hours
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Re: Recognizing and dealing with contamination [Re: MycoSnailCarl]
#28594276 - 12/22/23 04:32 PM (1 month, 5 days ago) |
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where should I take transfers from these two contaminated plates?

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Presidente
Stranger

Registered: 11/15/23
Posts: 21
Last seen: 7 days, 7 hours
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Re: Recognizing and dealing with contamination [Re: Presidente]
#28594321 - 12/22/23 05:08 PM (1 month, 5 days ago) |
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Premade agar plates looks like they are contaminated?
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Floret
Unmodify everything



Registered: 01/02/23
Posts: 260
Loc: underneath the water
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Re: Recognizing and dealing with contamination [Re: Presidente]
#28594672 - 12/23/23 01:18 AM (1 month, 5 days ago) |
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I would try 4-6 on the first plate with at least another transfer after that.
The premade plate is definitely contaminated.
-------------------- LAGM 2024
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DRAKENCL
Stranger
Registered: 02/02/23
Posts: 6
Last seen: 7 days, 16 hours
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Re: Recognizing and dealing with contamination [Re: Floret]
#28597103 - 12/24/23 09:11 PM (1 month, 3 days ago) |
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Hello, I have a question about my last boxes, the growth stagnates and they look like rot, I would like to know your opinions
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Edited by DRAKENCL (12/24/23 09:13 PM)
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,144
Loc: Stables
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Re: Recognizing and dealing with contamination [Re: DRAKENCL]
#28597106 - 12/24/23 09:13 PM (1 month, 3 days ago) |
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Super dry. You need to initiate heavy misting until it stops drinking, then maintain surface conditions. Reduce FAE if possible. Maybe even bottom water this. She THIRSTY
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DRAKENCL
Stranger
Registered: 02/02/23
Posts: 6
Last seen: 7 days, 16 hours
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#28597308 - 12/25/23 04:53 AM (1 month, 3 days ago) |
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Thanks for your help, repeat what you said. Now I have another question: should I use a hairdressing-type sprayer or a plant sprayer? I use the latter. Could it be that it hurts the buckets? Finally I show you a photo of the growth that I am trying to describe to you.
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DRAKENCL
Stranger
Registered: 02/02/23
Posts: 6
Last seen: 7 days, 16 hours
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Re: Recognizing and dealing with contamination [Re: DRAKENCL]
#28597309 - 12/25/23 04:55 AM (1 month, 3 days ago) |
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For humidity I use this tool, is it wrong?
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Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,396
Loc: Germany
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Re: Recognizing and dealing with contamination [Re: DRAKENCL]
#28597449 - 12/25/23 10:16 AM (1 month, 2 days ago) |
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You want fine mist for not hurting mycelium, and to leave the fine water pearls intact while landing on the surface. Hairdesser pumps are the best imo, but if you know what you´re doing you can use else.
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DRAKENCL
Stranger
Registered: 02/02/23
Posts: 6
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Re: Recognizing and dealing with contamination [Re: Goatrider]
#28597469 - 12/25/23 10:55 AM (1 month, 2 days ago) |
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Thank you very much for your opinion, I share it.
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Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,396
Loc: Germany
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Re: Recognizing and dealing with contamination [Re: DRAKENCL] 1
#28597473 - 12/25/23 11:05 AM (1 month, 2 days ago) |
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Forgot to mention to keep distance to the surface. A fine mister will also hurt myc when applied straight. Always mist from high above and let them pearls land smoothly.
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fnulnu
Student

Registered: 12/20/23
Posts: 15
Last seen: 1 day, 18 hours
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Re: Recognizing and dealing with contamination [Re: Goatrider]
#28610182 - 01/05/24 06:54 AM (23 days, 1 hour ago) |
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Hey now Made some agar plates with mea purchased online, inoculated a couple dozen, and the Ps cyanescens is doing that blue stuff, not sure what it is, as i thought trich was green, and that shit looks blue. Sorry bout the pic, brought the plates with me... What is it what caused it and where can I read more about stopping it. All other plates seem fine, but cyans/azis are what I want to innocuoate the world with Thank you
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,491
Loc: USA
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Re: Recognizing and dealing with contamination [Re: fnulnu]
#28610199 - 01/05/24 07:29 AM (23 days, 28 minutes ago) |
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Could be bruising due to stress. Could be bacteria under that or it’s just flat out mold. Do you have a better plate to work with
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Genome794
Spore-Whore-der & Agar-Addict


Registered: 07/20/22
Posts: 437
Loc: The Blue Planet
Last seen: 5 hours, 46 minutes
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Re: Recognizing and dealing with contamination [Re: fnulnu] 2
#28610272 - 01/05/24 09:11 AM (22 days, 22 hours ago) |
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Looks green to me. Could be the white balance of the pic.
You say you noc'd a dozen or so... If it were me, that plate would be dumped. I have never had anything good from a plate that looked like that.
Sorry
794
-------------------- Looking at myc on agar never gets old. LAGM-2023 Bigfootscreepyuncl - LAGM2023 - Challenge
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Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,396
Loc: Germany
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Re: Recognizing and dealing with contamination [Re: fnulnu] 3
#28610289 - 01/05/24 09:41 AM (22 days, 22 hours ago) |
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Quote:
fnulnu said:

Hey now Made some agar plates with mea purchased online, inoculated a couple dozen, and the Ps cyanescens is doing that blue stuff, not sure what it is, as i thought trich was green, and that shit looks blue. Sorry bout the pic, brought the plates with me... What is it what caused it and where can I read more about stopping it. All other plates seem fine, but cyans/azis are what I want to innocuoate the world with Thank you
To me that´s either penicillium or aspergillus.
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JW123
Nooby Nooby Noob


Registered: 01/17/22
Posts: 1,303
Loc: Tn
Last seen: 15 hours, 8 minutes
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Re: Recognizing and dealing with contamination [Re: Goatrider] 1
#28610563 - 01/05/24 02:04 PM (22 days, 17 hours ago) |
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Does anyone know what would cause mushrooms to turn into a slimey black mass in a day or two. I had to harvest my subtropicalis because whatever it is seems to be spreading. Should I worry about my other tubs?
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,655
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Re: Recognizing and dealing with contamination [Re: JW123] 2
#28610740 - 01/05/24 04:10 PM (22 days, 15 hours ago) |
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Looks wet af in there, likely bacterial, got mold creeping from the left.
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JW123
Nooby Nooby Noob


Registered: 01/17/22
Posts: 1,303
Loc: Tn
Last seen: 15 hours, 8 minutes
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Re: Recognizing and dealing with contamination [Re: LadysKnight]
#28610860 - 01/05/24 06:09 PM (22 days, 13 hours ago) |
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Thank you. I have been misting it daily to try to get them to produce spores, but maybe I am not giving them enough FAE.
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Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,727
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Re: Recognizing and dealing with contamination [Re: JW123] 2
#28610870 - 01/05/24 06:19 PM (22 days, 13 hours ago) |
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Just don't mist. It looks like mud in there
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
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JW123
Nooby Nooby Noob


Registered: 01/17/22
Posts: 1,303
Loc: Tn
Last seen: 15 hours, 8 minutes
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Re: Recognizing and dealing with contamination [Re: Gastronomicus]
#28610877 - 01/05/24 06:28 PM (22 days, 13 hours ago) |
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I haven't been able to get these things to produce spores. Misting daily is the advice for this species to get them to sporulate. I have more tubs going. I threw that one out. I will mist my next run less often and try to find the sweet spot. Thank you for the response.
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