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OfflineRockLicker
Yummy Yummy Rocks

Registered: 08/16/23
Posts: 21
Last seen: 11 days, 19 hours
Re: Recognizing and dealing with contamination [Re: huey.bluey]
    #28581642 - 12/13/23 05:49 PM (1 month, 14 days ago)

What are we looking at here? I made clones from PF tek and transferred them. How do they look? I was told by another user they appear to be mold. Would others agree with this? How can you tell it's mold and not tomentose growth if it is mold I've been transferring this whole time.
T2 of treasure coast

T3 of malabar coast


--------------------
Rockhounding and mycology are two very different fields; but for those who enjoy both nature is our ultimate playground. Keep playing, don’t let life wear you down. Best life advice I’ve gotten “Sometimes you need to slow down and lick the rocks”, these are wise words to live by.


Edited by RockLicker (12/13/23 05:50 PM)


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OfflineFuzzyness
Stranger
Registered: 04/21/23
Posts: 7
Last seen: 24 days, 23 hours
Re: Recognizing and dealing with contamination [Re: WyoMX]
    #28582082 - 12/14/23 01:52 AM (1 month, 14 days ago)

Quote:

WyoMX said:
Was it one later of micropore tape only? Also was it done in a Still air box at all or open air?




one layer of MP tape and not in a still air box.


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OfflineZakkery
I'm a teapot User Gallery


Registered: 10/15/18
Posts: 293
Loc: UK Flag
Last seen: 6 days, 3 hours
Re: Recognizing and dealing with contamination [Re: RockLicker]
    #28582084 - 12/14/23 01:54 AM (1 month, 14 days ago)







What am I actually growing here? I can't decide if it's actually cube myc or some sort of white mold.

They're from spore print>agar>millet. The agar plate smelled very mushroomy but did grow quite thick and fast. Growth on the agar looked ok considering the temps but I did loose some jars to clear contamination.


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OfflineAspectOfTheCreator
Mastering the Art of Success
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Registered: 12/07/22
Posts: 1,263
Last seen: 1 hour, 22 minutes
Re: Recognizing and dealing with contamination [Re: Zakkery]
    #28582204 - 12/14/23 06:14 AM (1 month, 14 days ago)

Does the box smell like mushrooms? If so youre good.


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OfflineKinoko314
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Registered: 12/16/22
Posts: 1,521
Loc: Colorado Flag
Last seen: 13 days, 15 hours
Re: Recognizing and dealing with contamination [Re: Zakkery] * 1
    #28582251 - 12/14/23 07:06 AM (1 month, 14 days ago)

Quote:

RockLicker said:
What are we looking at here? I made clones from PF tek and transferred them. How do they look? I was told by another user they appear to be mold. Would others agree with this? How can you tell it's mold and not tomentose growth if it is mold I've been transferring this whole time.
T2 of treasure coast

T3 of malabar coast





Some people will say that about any tomentose culture.  I think the 2nd one is fine, but the first one is more questionable.  I would keep transferring that one.


EDIT:  You know, I saw the APE clone pic in the QQ-QA thread.  I thought that myc looked as good as these (on the edges), but it looks like it's sporulating.  Unless that color was just from the actual mushroom tissue you were cloning.  This makes me much less sure about the other 2. 

It's almost impossible to tell the difference between tomentose cube myc and some white molds.  I'm sure some people have thrown away cube myc, and some people have grown a tub of mold.


Quote:

Zakkery said:

What am I actually growing here? I can't decide if it's actually cube myc or some sort of white mold.

They're from spore print>agar>millet. The agar plate smelled very mushroomy but did grow quite thick and fast. Growth on the agar looked ok considering the temps but I did loose some jars to clear contamination.




It looks like cube myc to me. 

I hope you're not sticking your face over an open plate to smell it.  Smell is a horrible first indicator.  If it smells bad, your contamination problems are major.  If it smells fine you could still have contamination.


Edited by Kinoko314 (12/14/23 08:47 AM)


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OfflineZakkery
I'm a teapot User Gallery


Registered: 10/15/18
Posts: 293
Loc: UK
Last seen: 6 days, 3 hours
Re: Recognizing and dealing with contamination [Re: Kinoko314]
    #28582685 - 12/14/23 02:22 PM (1 month, 13 days ago)

Quote:

Kinoko314 said:


It looks like cube myc to me. 

I hope you're not sticking your face over an open plate to smell it.  Smell is a horrible first indicator.  If it smells bad, your contamination problems are major.  If it smells fine you could still have contamination.




No, the smell test were on similar plates from the same batch that I didn't use. I was curious about the thicker growth and decided to give it a scratch and sniff out of curiosity and to get some kind of confirmation that it wasn't just mold.

I'll let the tubs ride and see what happens. Appreciate the feedback as always gents.


Edited by Zakkery (12/14/23 02:23 PM)


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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 8 hours, 16 minutes
Re: Recognizing and dealing with contamination [Re: AspectOfTheCreator]
    #28582687 - 12/14/23 02:24 PM (1 month, 13 days ago)

Quote:

AspectOfTheCreator said:
Does the box smell like mushrooms? If so youre good.




This is my goto for all boxes I'm unsure about


--------------------

Links For Beginners And Beyond


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OfflinePresidente
Stranger

Registered: 11/15/23
Posts: 21
Last seen: 7 days, 7 hours
Re: Recognizing and dealing with contamination [Re: rumfor69]
    #28583198 - 12/14/23 09:11 PM (1 month, 13 days ago)

What the fuck is this?!

I’m not sure if the pinkish/red is from the myc eating the agar food coloring or if I have a serious case of mold.

This is my clone plate:



This is my first transfer:



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OfflinePBJ710
Strangler

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Registered: 07/05/19
Posts: 2,623
Last seen: 17 days, 23 hours
Re: Recognizing and dealing with contamination [Re: Presidente] * 2
    #28583210 - 12/14/23 09:19 PM (1 month, 13 days ago)

Looks like 2 plates of mold to me.


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OfflineKinoko314
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Registered: 12/16/22
Posts: 1,521
Loc: Colorado Flag
Last seen: 13 days, 15 hours
Re: Recognizing and dealing with contamination [Re: PBJ710]
    #28583223 - 12/14/23 09:30 PM (1 month, 13 days ago)

I think any kind of myc can eat the food coloring.  That part is no big deal.

I'm also leaning toward those being mold.


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OfflinePresidente
Stranger

Registered: 11/15/23
Posts: 21
Last seen: 7 days, 7 hours
Re: Recognizing and dealing with contamination [Re: Kinoko314]
    #28583266 - 12/14/23 10:12 PM (1 month, 13 days ago)

Any ideas what kind of mold? I want to do some research because all my plates have the same characteristics; this one specific type of growth.

Edit: The “mold” isn’t growing exceptionally fast or anything. Second plate is approx. 12 days. First plate is approx 3 weeks.


Edited by Presidente (12/14/23 10:15 PM)


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OfflinePBJ710
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Re: Recognizing and dealing with contamination [Re: Presidente] * 1
    #28583278 - 12/14/23 10:24 PM (1 month, 13 days ago)

It's whatever mold spores are found in your home :shrug:  It really doesn't matter which specific mold it is as the root issue of poor sterile technique is to blame.

Is there a reason you took transfers from directly beside the original transfer without letting anything grow out first?


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OfflinePresidente
Stranger

Registered: 11/15/23
Posts: 21
Last seen: 7 days, 7 hours
Re: Recognizing and dealing with contamination [Re: PBJ710]
    #28583852 - 12/15/23 11:40 AM (1 month, 12 days ago)

Okay, I did another transfer. I’ve been flipping a tote on the side and doing my agar work in there. I’ll just flip it upside down next to a ledge and try it that way for my next transfer.

I took the transfer so close because it looked like healthy myc. I’m also new to this so still learning.

My “Tek” is:

Iso 70% working space (tops and walls)
Iso 70% drench tote x 2
Flip tote on side
Wipe down equipment (scapel, plates) with ISO 70%
Put plates in tote
Put scalpel in shot glass filled with ISO 70% after each use
Flame sterilize scalpel before each use



Edited by Presidente (12/15/23 11:44 AM)


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OfflineGastronomicus
3-0-G
Male User Gallery


Registered: 03/31/05
Posts: 9,727
Last seen: 1 day, 16 hours
Re: Recognizing and dealing with contamination [Re: Presidente]
    #28583940 - 12/15/23 01:19 PM (1 month, 12 days ago)

How concerning is this clear leading edge? I'm on the fence but my heart is saying it's a contam riding along



Some plates that were improperly sealed

Yeast



Bacteria



--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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InvisibleWay
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
Re: Recognizing and dealing with contamination [Re: Gastronomicus]
    #28584070 - 12/15/23 03:06 PM (1 month, 12 days ago)

That third pic of the clear edge doesn't look too bad after it grows out. I don't consider a clear leading edge as a cause for concern if the culture is otherwise healthy imo. No guarantee it's not bad but it seems to be pretty common ime.


--------------------

That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.


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Offlineboomerbob
Hologram
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Registered: 06/25/21
Posts: 22
Loc: Los Angeles, CA Flag
Last seen: 1 month, 11 days
Re: Recognizing and dealing with contamination [Re: Way]
    #28585260 - 12/16/23 11:34 AM (1 month, 11 days ago)

These are macro shots of a PE tub after four flushes and a couple of days after a dunk.  This patch appeared and grew to about 2 inches wide over the course of 24 hours.
Looks like I could have my first case of Trichoderma.  Can someone confirm?





--------------------





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OfflineKinoko314
Stranger Danger
Male

Registered: 12/16/22
Posts: 1,521
Loc: Colorado Flag
Last seen: 13 days, 15 hours
Re: Recognizing and dealing with contamination [Re: boomerbob]
    #28585324 - 12/16/23 12:26 PM (1 month, 11 days ago)

I think it most likely is.  I see a little green spot at 9 o'clock from the splotch.  Also if it's growing that fast it must be mold of some kind.

Something about it looks a little different to me than trich usually looks, but maybe I'm crazy.  Whatever mold it actually is, the problem is the same.


EDIT: Is your name boomerbob because you like boomers, or because you are a boomer?  Just curious.


Edited by Kinoko314 (12/16/23 12:37 PM)


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Offlineboomerbob
Hologram
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Registered: 06/25/21
Posts: 22
Loc: Los Angeles, CA
Last seen: 1 month, 11 days
Re: Recognizing and dealing with contamination [Re: Kinoko314]
    #28585330 - 12/16/23 12:33 PM (1 month, 11 days ago)

Quote:

Kinoko314 said:
I think it most likely is.  I see a little green spot at 9 o'clock from the splotch.  Also if it's growing that fast it must be mold of some kind.

Something about it looks a little different to me than trich usually looks, but maybe I'm crazy.  Whatever mold it actually is, the problem is the same.




Yes another small green splotch around 2 o'clock from main patch in second pic.


--------------------





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OfflineGastronomicus
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Registered: 03/31/05
Posts: 9,727
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Re: Recognizing and dealing with contamination [Re: boomerbob] * 1
    #28585430 - 12/16/23 02:00 PM (1 month, 11 days ago)

100% trich, that puffy white growth always goes green


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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OfflineHerman123
Stranger
Registered: 10/23/23
Posts: 56
Last seen: 18 hours, 13 minutes
Re: Recognizing and dealing with contamination [Re: Kizzle]
    #28585480 - 12/16/23 02:46 PM (1 month, 11 days ago)

Really helpful thank you


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