Home | Community | Message Board

World Seed Supply
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: < First | < Back | 240 | 241 | 242 | 243 | 244 | 245 | 246 | 247 | 248 | 249 | 250 | 251 | 252 | 253 | 254 | 255 | 256 | 257 | 258 | 259 | 260 | Next >
InvisibleWay
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
Re: Recognizing and dealing with contamination [Re: kengken]
    #28580527 - 12/12/23 08:36 PM (1 month, 15 days ago)

It's just bruising and yellowing from bad conditions. Very stressed mycelium.


--------------------

That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.


Extras: Filter Print Post Top
OfflineHugheGRection
Male

Registered: 02/04/21
Posts: 129
Loc: United States
Last seen: 9 hours, 10 minutes
Re: Recognizing and dealing with contamination [Re: kengken]
    #28580529 - 12/12/23 08:38 PM (1 month, 15 days ago)

Just looks like an abort or two and some bruising. Fruits won the race, keep an eye on it, but I don't think you should be concerned


--------------------
"The cost of Sanity, in this Society, is a certain level of alienation."


๐Ÿ…ƒ๐Ÿ„ด๐Ÿ„ฐ๐Ÿ„ผ ๐Ÿ„ฟ๐Ÿ„ฐ๐Ÿ…๐Ÿ„ฐ๐Ÿ„ต๐Ÿ„ธ๐Ÿ„ป๐Ÿ„ผ


Extras: Filter Print Post Top
OfflineFuzzyness
Stranger
Registered: 04/21/23
Posts: 7
Last seen: 24 days, 23 hours
Re: Recognizing and dealing with contamination [Re: HugheGRection]
    #28580804 - 12/13/23 05:57 AM (1 month, 15 days ago)

hia. I'm struggling with contamination. I'm trying PFTek with spores from liquid solution. my containers and rice flour/verm have been pressured cooked for 75 minutes. however, on the last 3 batches (with two different liquid cultures) i always seem to get the green mould appearing - please see picture.

i've followed the PFket guide on here and once inoculated just leave the tubs on a shelf. is it something i'm doing wrong with the steralisation or the innoculating or have i just been unlucky with the liquid cultures that i've bought (again i've tried a different supplier and the same has happened with both). any thoughts greatly appreciated.


Extras: Filter Print Post Top
OfflineKinoko314
Stranger Danger
Male

Registered: 12/16/22
Posts: 1,521
Loc: Colorado Flag
Last seen: 13 days, 15 hours
Re: Recognizing and dealing with contamination [Re: Fuzzyness]
    #28581022 - 12/13/23 08:54 AM (1 month, 14 days ago)

Quote:

Fuzzyness said:
hia. I'm struggling with contamination. I'm trying PFTek with spores from liquid solution. my containers and rice flour/verm have been pressured cooked for 75 minutes. however, on the last 3 batches (with two different liquid cultures) i always seem to get the green mould appearing - please see picture.

i've followed the PFket guide on here and once inoculated just leave the tubs on a shelf. is it something i'm doing wrong with the steralisation or the innoculating or have i just been unlucky with the liquid cultures that i've bought (again i've tried a different supplier and the same has happened with both). any thoughts greatly appreciated.





Spores or LC?  How did you actually do this?  It looks like you use a PP container instead of a jar.  What are you using as a filter on the lid?  How are you inoculating?

It may simply be that the syringes are contaminated, but more information would help us figure that out better.


Extras: Filter Print Post Top
OfflineSmellyhobbit
Actual Retard
Male User Gallery


Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 24 minutes, 22 seconds
Re: Recognizing and dealing with contamination [Re: Kinoko314]
    #28581060 - 12/13/23 09:58 AM (1 month, 14 days ago)

I have a question for you big brains out there concerning my recent bout with contam.

I have identified one change in procedure that might explain my problems. The only thing different about these batches vs the new ones is that I stopped scooping my sunflower seeds. Now that might not sound like a big issue, but these last two batches were bottom of the bucket grains, and they have an unusually high concentration of sunflower seeds anyway. It could be my imagination, but the jars that are going bad the fastest all appear to have a high volume of sunflower seeds in them vs the ones that donโ€™t.

My working theory right now is that my prep is good enough to finish hydrating and sterilizing a FEW sunflower seeds in the mixture, but not a proper prep if we are approaching 25%+ in mixture.

This isnโ€™t a problem in normal WBS blends because millet buffs out the composition by about 30%, but with millet removed I think itโ€™s possible this is creating uneven hydration.

Any thoughts?

Tomorrow Iโ€™m making 4 jars and removing the sunflower seeds as I used to and Iโ€™ll see what happens.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



Extras: Filter Print Post Top
OfflineKinoko314
Stranger Danger
Male

Registered: 12/16/22
Posts: 1,521
Loc: Colorado Flag
Last seen: 13 days, 15 hours
Re: Recognizing and dealing with contamination [Re: Smellyhobbit] * 1
    #28581077 - 12/13/23 10:30 AM (1 month, 14 days ago)

It seems reasonable to me.  I would assume sunflower seeds would be harder to hydrate than the smaller seeds.

I usually take out almost all the sunflower seeds from my WBS, so I wouldn't know from experience.

Do let us know how that turns out.


Extras: Filter Print Post Top
OfflineJW123
Nooby Nooby Noob
 User Gallery

Registered: 01/17/22
Posts: 1,303
Loc: Tn
Last seen: 15 hours, 8 minutes
Re: Recognizing and dealing with contamination [Re: Kinoko314] * 3
    #28581096 - 12/13/23 10:46 AM (1 month, 14 days ago)

All this discussion is why smelly will always be a better cultivator than me. My second to last grain run went horribly wrong. Did I ask for help? Did I try to discover flaws in my process?
:whatnonono:
Just gonna do more jars next time I said to myself. Now this run goes off without a hitch. I still don't know what happened last time and I don't have room for all my tubs. Don't be like me kids.


Extras: Filter Print Post Top
OfflineFuzzyness
Stranger
Registered: 04/21/23
Posts: 7
Last seen: 24 days, 23 hours
Re: Recognizing and dealing with contamination [Re: Kinoko314]
    #28581143 - 12/13/23 11:25 AM (1 month, 14 days ago)

hia.
Quote:

Smellyhobbit said:
I have a question for you big brains out there concerning my recent bout with contam.

I have identified one change in procedure that might explain my problems. The only thing different about these batches vs the new ones is that I stopped scooping my sunflower seeds. Now that might not sound like a big issue, but these last two batches were bottom of the bucket grains, and they have an unusually high concentration of sunflower seeds anyway. It could be my imagination, but the jars that are going bad the fastest all appear to have a high volume of sunflower seeds in them vs the ones that donโ€™t.

My working theory right now is that my prep is good enough to finish hydrating and sterilizing a FEW sunflower seeds in the mixture, but not a proper prep if we are approaching 25%+ in mixture.

This isnโ€™t a problem in normal WBS blends because millet buffs out the composition by about 30%, but with millet removed I think itโ€™s possible this is creating uneven hydration.

Any thoughts?

Tomorrow Iโ€™m making 4 jars and removing the sunflower seeds as I used to and Iโ€™ll see what happens.



Quote:

Kinoko314 said:
Quote:

Fuzzyness said:
hia. I'm struggling with contamination. I'm trying PFTek with spores from liquid solution. my containers and rice flour/verm have been pressured cooked for 75 minutes. however, on the last 3 batches (with two different liquid cultures) i always seem to get the green mould appearing - please see picture.

i've followed the PFket guide on here and once inoculated just leave the tubs on a shelf. is it something i'm doing wrong with the steralisation or the innoculating or have i just been unlucky with the liquid cultures that i've bought (again i've tried a different supplier and the same has happened with both). any thoughts greatly appreciated.





Spores or LC?  How did you actually do this?  It looks like you use a PP container instead of a jar.  What are you using as a filter on the lid?  How are you inoculating?

It may simply be that the syringes are contaminated, but more information would help us figure that out better.




so i mixed my rice flour with verm and water, then put in plastic containers (fast food takeaway containers). the holes were covered with micro pore tape when they went into the pressure cooker. i then put the container in a pressure cooker for 75 minutes. once cooled (48 hours later), i then injected spores which came in liquid in a syringe. using 4 small holes in each corner of the lid. once the solution had been injected i recovered the holes with micropore tape and left. teh green stuff started appearing about 4 days after the tray had been inoculated.


Extras: Filter Print Post Top
InvisibleWyoMX
I'm a teapot User Gallery
Registered: 07/06/15
Posts: 2,100
Loc: PNW
Re: Recognizing and dealing with contamination [Re: Fuzzyness]
    #28581152 - 12/13/23 11:31 AM (1 month, 14 days ago)

Was it one later of micropore tape only? Also was it done in a Still air box at all or open air?


Extras: Filter Print Post Top
OfflineSmellyhobbit
Actual Retard
Male User Gallery


Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 24 minutes, 22 seconds
Re: Recognizing and dealing with contamination [Re: WyoMX]
    #28581242 - 12/13/23 12:24 PM (1 month, 14 days ago)

Did you include a dry verm barrier? If so, the MP tape is unnecessary. Also I would personally cook PF substrate for 90 minutes.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



Extras: Filter Print Post Top
OfflineKinoko314
Stranger Danger
Male

Registered: 12/16/22
Posts: 1,521
Loc: Colorado Flag
Last seen: 13 days, 15 hours
Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #28581286 - 12/13/23 01:22 PM (1 month, 14 days ago)

Quote:

Smellyhobbit said:
Did you include a dry verm barrier? If so, the MP tape is unnecessary. Also I would personally cook PF substrate for 90 minutes.



It's normally 90 minutes if you steam it.  Crazy, I know.  I hadn't done PF tek for a long time, so when I wanted to make some pucks I went back to check.  In fact I just checked again now and what he's doing is all in there as an option.



I don't like single layers of micropore.  2-3 layers would be much safer.  If you're not heating your needle red hot before inoculating that could be a vector too.

It's hard to say if mold is getting in through the poor filter that a single layer of MP tape makes, or if it's happening during inoculation, or if it's actually in the syringe itself.

Try more tape and heating your needle during innoc and see if that fixes things.


Extras: Filter Print Post Top
InvisibleWay
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #28581359 - 12/13/23 02:08 PM (1 month, 14 days ago)

Quote:

Smellyhobbit said:
I have a question for you big brains out there concerning my recent bout with contam.

I have identified one change in procedure that might explain my problems. The only thing different about these batches vs the new ones is that I stopped scooping my sunflower seeds. Now that might not sound like a big issue, but these last two batches were bottom of the bucket grains, and they have an unusually high concentration of sunflower seeds anyway. It could be my imagination, but the jars that are going bad the fastest all appear to have a high volume of sunflower seeds in them vs the ones that donโ€™t.

My working theory right now is that my prep is good enough to finish hydrating and sterilizing a FEW sunflower seeds in the mixture, but not a proper prep if we are approaching 25%+ in mixture.

This isnโ€™t a problem in normal WBS blends because millet buffs out the composition by about 30%, but with millet removed I think itโ€™s possible this is creating uneven hydration.

Any thoughts?

Tomorrow Iโ€™m making 4 jars and removing the sunflower seeds as I used to and Iโ€™ll see what happens.




You said you got contam over your last 4 grain preps. Does that mean for those 4 preps you were using a higher concentration of sunflower seeds?

The logic does check out for me but you also said LC works fine on grains, right?


--------------------

That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.


Extras: Filter Print Post Top
OfflineSmellyhobbit
Actual Retard
Male User Gallery


Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 24 minutes, 22 seconds
Re: Recognizing and dealing with contamination [Re: Way]
    #28581409 - 12/13/23 02:35 PM (1 month, 14 days ago)

Yes to both.

I started thinking today and looking at dates. My A2G jars are going bad 2-3 days after a LC jar would be just finishing up. Up until that they look fine. Iโ€™ll shake them and then they wonโ€™t recover. What if the LC is just getting ahead of whatever is germing in those sunflower seeds?


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



Extras: Filter Print Post Top
InvisibleWay
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #28581440 - 12/13/23 02:59 PM (1 month, 14 days ago)

Sounds entirely possible to me friend. Switch it up and let us know what happens.


--------------------

That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.


Extras: Filter Print Post Top
InvisibleLadyBird123S


Registered: 09/27/22
Posts: 257
Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #28581507 - 12/13/23 04:09 PM (1 month, 14 days ago)

Been my thoughts on this matter. :wink: sketch ass sunflower seeds.


Extras: Filter Print Post Top
OfflineNonagon
Bacon frying, sparrows chirping
 User Gallery

Registered: 09/01/22
Posts: 1,077
Loc: Hyperspace
Last seen: 23 hours, 3 minutes
Re: Recognizing and dealing with contamination [Re: LadyBird123]
    #28581543 - 12/13/23 04:34 PM (1 month, 14 days ago)

Yo smelly, all my jars look pretty darn good rn except for one spot, in one jar, on a sunflower seed.


--------------------
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ  ๐Ÿ„ฒ ๐Ÿ… ๐Ÿ…„ ๐Ÿ„ฝ ๐Ÿ„ฒ ๐Ÿ„ท ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


Extras: Filter Print Post Top
OfflineSmellyhobbit
Actual Retard
Male User Gallery


Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 24 minutes, 22 seconds
Re: Recognizing and dealing with contamination [Re: Nonagon]
    #28581570 - 12/13/23 04:55 PM (1 month, 14 days ago)

Quote:

LadyBird123 said:
Been my thoughts on this matter. :wink: sketch ass sunflower seeds.



Well I now hate them more than millet. Iโ€™ve always been told theyโ€™re not a problem, and thatโ€™s probably mostly true. But Iโ€™m gonna take them out and see what happens.

Quote:

Nonagon said:
Yo smelly, all my jars look pretty darn good rn except for one spot, in one jar, on a sunflower seed.



Guess we just gotta scoop em, man. Go figure


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



Extras: Filter Print Post Top
Onlinehuey.bluey
I'm a teapot Unread Journal User Gallery

Registered: 01/16/19
Posts: 1,447
Loc: Mississippi River
Last seen: 7 seconds
Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #28581578 - 12/13/23 05:01 PM (1 month, 14 days ago)

Rye :ancientaliens:


--------------------
:mushroom2: huey.bluey's Trading List


Extras: Filter Print Post Top
OfflineSmellyhobbit
Actual Retard
Male User Gallery


Registered: 04/01/22
Posts: 11,144
Loc: Stables
Last seen: 24 minutes, 22 seconds
Re: Recognizing and dealing with contamination [Re: huey.bluey] * 1
    #28581583 - 12/13/23 05:05 PM (1 month, 14 days ago)

Quote:

huey.bluey said:
Rye :ancientaliens:



Bye ๐Ÿ‘‹


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



Extras: Filter Print Post Top
Onlinehuey.bluey
I'm a teapot Unread Journal User Gallery

Registered: 01/16/19
Posts: 1,447
Loc: Mississippi River
Last seen: 7 seconds
Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #28581635 - 12/13/23 05:43 PM (1 month, 14 days ago)

Fry the best spawn is a Yukon potata


--------------------
:mushroom2: huey.bluey's Trading List


Edited by huey.bluey (12/13/23 05:44 PM)


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 240 | 241 | 242 | 243 | 244 | 245 | 246 | 247 | 248 | 249 | 250 | 251 | 252 | 253 | 254 | 255 | 256 | 257 | 258 | 259 | 260 | Next >

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Contamination + Bleach tek = ? anonymoushate 2,706 7 07/04/02 02:37 AM
by Hippie3
* CONTAMINATION!!! HELP!!!!! trichoderma,apergillus niger iopener 1,943 8 09/29/03 01:37 AM
by OJ
* Contamination, very minor area - still a major problem? HerbanShaman 830 2 04/10/05 09:53 PM
by HerbanShaman
* Contaminated casing - edibility..? (pics) BitchCakes 3,016 8 12/04/02 08:01 AM
by NeonBlack
* Smelt Jars for contamination. I was taken to ER!!!
( 1 2 3 4 5 6 all )
JayBin 42,882 110 01/15/21 06:15 AM
by bodhisatta
* Niger Seed Kalix 1,420 10 07/07/05 01:55 AM
by Kalix
* May be contaminated? AKMycelium 176 7 01/23/24 10:11 PM
by HelloImBob
* Purple/Pink contamination can be DEADLY
( 1 2 3 all )
Ryche HawkV 32,498 42 04/23/22 11:05 PM
by Imatryin

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
422,979 topic views. 35 members, 209 guests and 66 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.011 seconds on 13 queries.