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Way
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
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Re: Recognizing and dealing with contamination [Re: kengken]
#28580527 - 12/12/23 08:36 PM (1 month, 15 days ago) |
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It's just bruising and yellowing from bad conditions. Very stressed mycelium.
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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.
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HugheGRection


Registered: 02/04/21
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Re: Recognizing and dealing with contamination [Re: kengken]
#28580529 - 12/12/23 08:38 PM (1 month, 15 days ago) |
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Just looks like an abort or two and some bruising. Fruits won the race, keep an eye on it, but I don't think you should be concerned
-------------------- "The cost of Sanity, in this Society, is a certain level of alienation."
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Fuzzyness
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Re: Recognizing and dealing with contamination [Re: HugheGRection]
#28580804 - 12/13/23 05:57 AM (1 month, 15 days ago) |
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hia. I'm struggling with contamination. I'm trying PFTek with spores from liquid solution. my containers and rice flour/verm have been pressured cooked for 75 minutes. however, on the last 3 batches (with two different liquid cultures) i always seem to get the green mould appearing - please see picture.
i've followed the PFket guide on here and once inoculated just leave the tubs on a shelf. is it something i'm doing wrong with the steralisation or the innoculating or have i just been unlucky with the liquid cultures that i've bought (again i've tried a different supplier and the same has happened with both). any thoughts greatly appreciated.
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Kinoko314
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Re: Recognizing and dealing with contamination [Re: Fuzzyness]
#28581022 - 12/13/23 08:54 AM (1 month, 14 days ago) |
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Quote:
Fuzzyness said: hia. I'm struggling with contamination. I'm trying PFTek with spores from liquid solution. my containers and rice flour/verm have been pressured cooked for 75 minutes. however, on the last 3 batches (with two different liquid cultures) i always seem to get the green mould appearing - please see picture.
i've followed the PFket guide on here and once inoculated just leave the tubs on a shelf. is it something i'm doing wrong with the steralisation or the innoculating or have i just been unlucky with the liquid cultures that i've bought (again i've tried a different supplier and the same has happened with both). any thoughts greatly appreciated.

Spores or LC? How did you actually do this? It looks like you use a PP container instead of a jar. What are you using as a filter on the lid? How are you inoculating?
It may simply be that the syringes are contaminated, but more information would help us figure that out better.
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: Kinoko314]
#28581060 - 12/13/23 09:58 AM (1 month, 14 days ago) |
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I have a question for you big brains out there concerning my recent bout with contam.
I have identified one change in procedure that might explain my problems. The only thing different about these batches vs the new ones is that I stopped scooping my sunflower seeds. Now that might not sound like a big issue, but these last two batches were bottom of the bucket grains, and they have an unusually high concentration of sunflower seeds anyway. It could be my imagination, but the jars that are going bad the fastest all appear to have a high volume of sunflower seeds in them vs the ones that donโt.
My working theory right now is that my prep is good enough to finish hydrating and sterilizing a FEW sunflower seeds in the mixture, but not a proper prep if we are approaching 25%+ in mixture.
This isnโt a problem in normal WBS blends because millet buffs out the composition by about 30%, but with millet removed I think itโs possible this is creating uneven hydration.
Any thoughts?
Tomorrow Iโm making 4 jars and removing the sunflower seeds as I used to and Iโll see what happens.
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Kinoko314
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit] 1
#28581077 - 12/13/23 10:30 AM (1 month, 14 days ago) |
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It seems reasonable to me. I would assume sunflower seeds would be harder to hydrate than the smaller seeds.
I usually take out almost all the sunflower seeds from my WBS, so I wouldn't know from experience.
Do let us know how that turns out.
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JW123
Nooby Nooby Noob


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Re: Recognizing and dealing with contamination [Re: Kinoko314] 3
#28581096 - 12/13/23 10:46 AM (1 month, 14 days ago) |
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All this discussion is why smelly will always be a better cultivator than me. My second to last grain run went horribly wrong. Did I ask for help? Did I try to discover flaws in my process?
 Just gonna do more jars next time I said to myself. Now this run goes off without a hitch. I still don't know what happened last time and I don't have room for all my tubs. Don't be like me kids.
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Fuzzyness
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Re: Recognizing and dealing with contamination [Re: Kinoko314]
#28581143 - 12/13/23 11:25 AM (1 month, 14 days ago) |
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hia. Quote:
Smellyhobbit said: I have a question for you big brains out there concerning my recent bout with contam.
I have identified one change in procedure that might explain my problems. The only thing different about these batches vs the new ones is that I stopped scooping my sunflower seeds. Now that might not sound like a big issue, but these last two batches were bottom of the bucket grains, and they have an unusually high concentration of sunflower seeds anyway. It could be my imagination, but the jars that are going bad the fastest all appear to have a high volume of sunflower seeds in them vs the ones that donโt.
My working theory right now is that my prep is good enough to finish hydrating and sterilizing a FEW sunflower seeds in the mixture, but not a proper prep if we are approaching 25%+ in mixture.
This isnโt a problem in normal WBS blends because millet buffs out the composition by about 30%, but with millet removed I think itโs possible this is creating uneven hydration.
Any thoughts?
Tomorrow Iโm making 4 jars and removing the sunflower seeds as I used to and Iโll see what happens.
Quote:
Kinoko314 said:
Quote:
Fuzzyness said: hia. I'm struggling with contamination. I'm trying PFTek with spores from liquid solution. my containers and rice flour/verm have been pressured cooked for 75 minutes. however, on the last 3 batches (with two different liquid cultures) i always seem to get the green mould appearing - please see picture.
i've followed the PFket guide on here and once inoculated just leave the tubs on a shelf. is it something i'm doing wrong with the steralisation or the innoculating or have i just been unlucky with the liquid cultures that i've bought (again i've tried a different supplier and the same has happened with both). any thoughts greatly appreciated.

Spores or LC? How did you actually do this? It looks like you use a PP container instead of a jar. What are you using as a filter on the lid? How are you inoculating?
It may simply be that the syringes are contaminated, but more information would help us figure that out better.
so i mixed my rice flour with verm and water, then put in plastic containers (fast food takeaway containers). the holes were covered with micro pore tape when they went into the pressure cooker. i then put the container in a pressure cooker for 75 minutes. once cooled (48 hours later), i then injected spores which came in liquid in a syringe. using 4 small holes in each corner of the lid. once the solution had been injected i recovered the holes with micropore tape and left. teh green stuff started appearing about 4 days after the tray had been inoculated.
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WyoMX

Registered: 07/06/15
Posts: 2,100
Loc: PNW
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Re: Recognizing and dealing with contamination [Re: Fuzzyness]
#28581152 - 12/13/23 11:31 AM (1 month, 14 days ago) |
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Was it one later of micropore tape only? Also was it done in a Still air box at all or open air?
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: WyoMX]
#28581242 - 12/13/23 12:24 PM (1 month, 14 days ago) |
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Did you include a dry verm barrier? If so, the MP tape is unnecessary. Also I would personally cook PF substrate for 90 minutes.
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Kinoko314
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#28581286 - 12/13/23 01:22 PM (1 month, 14 days ago) |
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Quote:
Smellyhobbit said: Did you include a dry verm barrier? If so, the MP tape is unnecessary. Also I would personally cook PF substrate for 90 minutes.
It's normally 90 minutes if you steam it. Crazy, I know. I hadn't done PF tek for a long time, so when I wanted to make some pucks I went back to check. In fact I just checked again now and what he's doing is all in there as an option.
I don't like single layers of micropore. 2-3 layers would be much safer. If you're not heating your needle red hot before inoculating that could be a vector too.
It's hard to say if mold is getting in through the poor filter that a single layer of MP tape makes, or if it's happening during inoculation, or if it's actually in the syringe itself.
Try more tape and heating your needle during innoc and see if that fixes things.
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Way
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#28581359 - 12/13/23 02:08 PM (1 month, 14 days ago) |
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Quote:
Smellyhobbit said: I have a question for you big brains out there concerning my recent bout with contam.
I have identified one change in procedure that might explain my problems. The only thing different about these batches vs the new ones is that I stopped scooping my sunflower seeds. Now that might not sound like a big issue, but these last two batches were bottom of the bucket grains, and they have an unusually high concentration of sunflower seeds anyway. It could be my imagination, but the jars that are going bad the fastest all appear to have a high volume of sunflower seeds in them vs the ones that donโt.
My working theory right now is that my prep is good enough to finish hydrating and sterilizing a FEW sunflower seeds in the mixture, but not a proper prep if we are approaching 25%+ in mixture.
This isnโt a problem in normal WBS blends because millet buffs out the composition by about 30%, but with millet removed I think itโs possible this is creating uneven hydration.
Any thoughts?
Tomorrow Iโm making 4 jars and removing the sunflower seeds as I used to and Iโll see what happens.
You said you got contam over your last 4 grain preps. Does that mean for those 4 preps you were using a higher concentration of sunflower seeds?
The logic does check out for me but you also said LC works fine on grains, right?
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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: Way]
#28581409 - 12/13/23 02:35 PM (1 month, 14 days ago) |
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Yes to both.
I started thinking today and looking at dates. My A2G jars are going bad 2-3 days after a LC jar would be just finishing up. Up until that they look fine. Iโll shake them and then they wonโt recover. What if the LC is just getting ahead of whatever is germing in those sunflower seeds?
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Way
The


Registered: 01/14/23
Posts: 4,336
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#28581440 - 12/13/23 02:59 PM (1 month, 14 days ago) |
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Sounds entirely possible to me friend. Switch it up and let us know what happens.
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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.
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LadyBird123


Registered: 09/27/22
Posts: 257
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#28581507 - 12/13/23 04:09 PM (1 month, 14 days ago) |
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Been my thoughts on this matter. sketch ass sunflower seeds.
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Nonagon
Bacon frying, sparrows chirping


Registered: 09/01/22
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Re: Recognizing and dealing with contamination [Re: LadyBird123]
#28581543 - 12/13/23 04:34 PM (1 month, 14 days ago) |
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Yo smelly, all my jars look pretty darn good rn except for one spot, in one jar, on a sunflower seed.
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: Nonagon]
#28581570 - 12/13/23 04:55 PM (1 month, 14 days ago) |
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Quote:
LadyBird123 said: Been my thoughts on this matter. sketch ass sunflower seeds.
Well I now hate them more than millet. Iโve always been told theyโre not a problem, and thatโs probably mostly true. But Iโm gonna take them out and see what happens.
Quote:
Nonagon said: Yo smelly, all my jars look pretty darn good rn except for one spot, in one jar, on a sunflower seed.
Guess we just gotta scoop em, man. Go figure
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huey.bluey


Registered: 01/16/19
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#28581578 - 12/13/23 05:01 PM (1 month, 14 days ago) |
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Rye
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: huey.bluey] 1
#28581583 - 12/13/23 05:05 PM (1 month, 14 days ago) |
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Quote:
huey.bluey said: Rye 
Bye ๐
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huey.bluey


Registered: 01/16/19
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#28581635 - 12/13/23 05:43 PM (1 month, 14 days ago) |
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Fry the best spawn is a Yukon potata
Edited by huey.bluey (12/13/23 05:44 PM)
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