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OfflineRedMustache
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Registered: 11/29/23
Posts: 2
Last seen: 1 month, 22 days
Re: Recognizing and dealing with contamination [Re: beezlebuffo]
    #28560811 - 11/29/23 01:37 PM (1 month, 28 days ago)

Hey guys so I did 2 monotubs everything went well up until fruiting then.... I fu##ed up low temps then bad air flow and in the dark while I was away for a few days.
There was a few flopped over skinny GT'S and white bumps that wanted to be...
I harvested a few and now don't know if I have some contam or it's just bruising what we think


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OfflineRedMustache
Stranger
Registered: 11/29/23
Posts: 2
Last seen: 1 month, 22 days
Re: Recognizing and dealing with contamination [Re: RedMustache]
    #28560817 - 11/29/23 01:40 PM (1 month, 28 days ago)





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Offlinewarrlok138
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Registered: 10/10/23
Posts: 63
Last seen: 11 days, 18 hours
Re: Recognizing and dealing with contamination [Re: RedMustache]
    #28561436 - 11/29/23 09:49 PM (1 month, 28 days ago)

Hello. What do you guys think this green spot is? I have thrown this cake away I am just curious what it could be.




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OfflineNichrome
I'm a torso!
I'm a teapot User Gallery

Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
Last seen: 7 hours, 34 minutes
Trusted Cultivator
Re: Recognizing and dealing with contamination [Re: warrlok138] * 1
    #28561550 - 11/30/23 12:05 AM (1 month, 28 days ago)

Trichoderma.


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineZwinst
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Registered: 02/27/21
Posts: 126
Loc: Germany
Last seen: 3 days, 21 hours
Re: Recognizing and dealing with contamination [Re: Kizzle]
    #28561585 - 11/30/23 01:50 AM (1 month, 28 days ago)

Here is something i haven´t seen before. At first it looks like the usual green mold, but it never changes colour.







Any idea what it could be? I guess it had to come from the latest batch of coir. After i lost everything to fungus gnats a short while ago, i sealed all boxes with cling wrap. And now this?! :mad2:

I guess by completely sealing the boxes i created a even better environment for mold.


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OfflineKinoko314
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Registered: 12/16/22
Posts: 1,521
Loc: Colorado Flag
Last seen: 13 days, 15 hours
Re: Recognizing and dealing with contamination [Re: Zwinst]
    #28563101 - 11/30/23 11:20 PM (1 month, 27 days ago)

Quote:

Zwinst said:
I guess by completely sealing the boxes i created a even better environment for mold.




You said it.  If you need to keep gnats out, put micropore tape over your monotub holes.  That way it can still get some air.  Whatever you're growing is pretty scary looking.  It looks like it's trying to crawl out of the tub to get me!

If the mold was in your coir, dumping boiling water on it when hydrating it will kill all the mold.  If you didn't do that, start doing that.  If you did do that, the mold came from elsewhere.  Probably just floating around the environment.


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OfflineZwinst
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Registered: 02/27/21
Posts: 126
Loc: Germany
Last seen: 3 days, 21 hours
Re: Recognizing and dealing with contamination [Re: Kinoko314]
    #28563254 - 12/01/23 03:18 AM (1 month, 27 days ago)

I always pasteurize the coir. However sometimes it seems to not be enough. Endospores i guess.

Everything i cased with the last batch of coir is contaminated. The few casings i made earlier the same day with the prior batch is still clean. Very curious.

I will try to take some macro photos later today. This is a very nasty contamination. I have to throw out 27 casings. :thumbdown:


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OfflineDERRAYLD
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Registered: 05/13/02
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Loc: South Africa
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Re: Recognizing and dealing with contamination [Re: Zwinst]
    #28563326 - 12/01/23 06:14 AM (1 month, 27 days ago)

By pasteurize do you mean a bucket with boiling water?


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OfflineZwinst
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Registered: 02/27/21
Posts: 126
Loc: Germany
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Re: Recognizing and dealing with contamination [Re: DERRAYLD]
    #28563466 - 12/01/23 09:02 AM (1 month, 26 days ago)

Yes. I just dump lots of boiling water on the bricks, let it soak and press the water out using a cloth bag after it cooled down.
I rarely have any contaminations, except for the occasional yeast from dirty spores, so this case is especially puzzling to me. It did everything the way i usually do it.


I tried my best to get some useful pictures of the culprit. Of course i have absolutely no idea what to look for when it comes to mold...





All i can tell is that there are lots of spores and they seem to be white or colourless.
What is it with me, that everything starts producing clear spores?! :rolleyes:


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OfflineDirtbaggery


Registered: 09/20/23
Posts: 59
Loc: LIGMA
Last seen: 7 days, 11 hours
Re: Recognizing and dealing with contamination [Re: Zwinst]
    #28566864 - 12/03/23 02:45 PM (1 month, 24 days ago)

Am I about to lose this tub to trich? Is there anything I can do to combat this?


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OfflineZwinst
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Registered: 02/27/21
Posts: 126
Loc: Germany
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Re: Recognizing and dealing with contamination [Re: Dirtbaggery] * 1
    #28566935 - 12/03/23 03:40 PM (1 month, 24 days ago)

You already lost it. Trying to cut it out wont work, it will come back in 2 days or so.

If you are desperate harvest the couple of big ones, but do discard the rest before everything turns green.

edit: Very nice looking shrooms btw. What are they?


Edited by Zwinst (12/03/23 03:42 PM)


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OfflineDirtbaggery


Registered: 09/20/23
Posts: 59
Loc: LIGMA
Last seen: 7 days, 11 hours
Re: Recognizing and dealing with contamination [Re: Zwinst]
    #28567186 - 12/03/23 05:56 PM (1 month, 24 days ago)

Quote:

Zwinst said:
You already lost it. Trying to cut it out wont work, it will come back in 2 days or so.

If you are desperate harvest the couple of big ones, but do discard the rest before everything turns green.

edit: Very nice looking shrooms btw. What are they?





Bummer. Thank you for the response. This is the second flush of some Thai Lipa Yai.


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OfflineSentiklos
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Registered: 11/24/22
Posts: 54
Loc: Southeastern Europe
Last seen: 1 day, 5 hours
Re: Recognizing and dealing with contamination [Re: Dirtbaggery]
    #28567862 - 12/04/23 06:43 AM (1 month, 24 days ago)

Wow. Best guide I've read so far. Nice work.


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InvisibleBigwormS
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Registered: 03/19/22
Posts: 1,868
Loc: Murica
Re: Recognizing and dealing with contamination [Re: Sentiklos]
    #28568088 - 12/04/23 10:26 AM (1 month, 23 days ago)

I may have found another vector for contamination. It's not a big vector and I haven't seen anyone mention it but I noticed it. While prepping grain I found some dry grain getting stuck around the rim of my strainer from mixing it up while rinsing the grain.
I use this type of strainer

And here is the lip in question.

I thought about it and when I dump my properly hydrated grain into the strainer a few dry grains my be getting released from the rim into them. It's probably never a lot, but I'm assuming a few dozen dry grains getting into the prepped grain might be carrying endospores. Being dry they might not get fully hydrated by the prepped grain during the PC cycle and even if they do, I suspect they aren't getting sterilized properly.
I'm not sure this is 100% correct just an assumption and another place to look for a possible contamination point.


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OfflineBrokenHeart
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Registered: 02/27/23
Posts: 127
Last seen: 3 hours, 5 minutes
Re: Recognizing and dealing with contamination [Re: Bigworm]
    #28571490 - 12/06/23 12:22 PM (1 month, 21 days ago)

Has the cube figured out how to drink from the water tub or is it contamination?



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OfflineKinoko314
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Registered: 12/16/22
Posts: 1,521
Loc: Colorado Flag
Last seen: 13 days, 15 hours
Re: Recognizing and dealing with contamination [Re: BrokenHeart] * 1
    #28571601 - 12/06/23 02:02 PM (1 month, 21 days ago)

Quote:

Zwinst said:
Yes. I just dump lots of boiling water on the bricks, let it soak and press the water out using a cloth bag after it cooled down.
I rarely have any contaminations, except for the occasional yeast from dirty spores, so this case is especially puzzling to me. It did everything the way i usually do it.




So you're using some cloth bag that's probably covered in mold spores from the regular environment and touching your coir with it?  I bet that's where it's coming from.  Either put the proper amount of water in so you don't have any to squeeze out, or use your hands or something else you can clean and is non-porous to get the water out.

If you use eco earth, it's 3.5x.  1000 g of coir to 3500 ml(grams) of water.
If you use coco bliss, it's 4.7x.
If you use something else I don't know if I can help you, but you can experiment to figure it out for your coir too.


Quote:

Bigworm said:
I may have found another vector for contamination. It's not a big vector and I haven't seen anyone mention it but I noticed it. While prepping grain I found some dry grain getting stuck around the rim of my strainer from mixing it up while rinsing the grain.
I use this type of strainer

And here is the lip in question.

I thought about it and when I dump my properly hydrated grain into the strainer a few dry grains my be getting released from the rim into them. It's probably never a lot, but I'm assuming a few dozen dry grains getting into the prepped grain might be carrying endospores. Being dry they might not get fully hydrated by the prepped grain during the PC cycle and even if they do, I suspect they aren't getting sterilized properly.
I'm not sure this is 100% correct just an assumption and another place to look for a possible contamination point.




It sounds like a reasonable hypothesis.  I would either use a different one or try to seal that one up. 


Quote:

BrokenHeart said:
Has the cube figured out how to drink from the water tub or is it contamination?






It looks like mold.  I would get rid of it.  Sorry for your loss.


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OfflineMycoSnailCarl
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Registered: 11/22/23
Posts: 181
Last seen: 1 day, 10 hours
Re: Recognizing and dealing with contamination [Re: Zwinst]
    #28571733 - 12/06/23 04:17 PM (1 month, 21 days ago)

Quote:

Zwinst said:
You already lost it. Trying to cut it out wont work, it will come back in 2 days or so.

If you are desperate harvest the couple of big ones, but do discard the rest before everything turns green.

edit: Very nice looking shrooms btw. What are they?




Any reason for him not to harvest the smaller ones as well? Will they not have developed actives yet?


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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,655
Re: Recognizing and dealing with contamination [Re: MycoSnailCarl]
    #28572466 - 12/07/23 07:21 AM (1 month, 21 days ago)

Quote:

MycoSnailCarl said:
Quote:

Zwinst said:
You already lost it. Trying to cut it out wont work, it will come back in 2 days or so.

If you are desperate harvest the couple of big ones, but do discard the rest before everything turns green.

edit: Very nice looking shrooms btw. What are they?




Any reason for him not to harvest the smaller ones as well? Will they not have developed actives yet?




All are good to pick


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OfflineMycoSnailCarl
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Registered: 11/22/23
Posts: 181
Last seen: 1 day, 10 hours
Re: Recognizing and dealing with contamination [Re: LadysKnight]
    #28572468 - 12/07/23 07:24 AM (1 month, 21 days ago)

Great, thank you


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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,655
Re: Recognizing and dealing with contamination [Re: MycoSnailCarl]
    #28572469 - 12/07/23 07:25 AM (1 month, 21 days ago)

:cheers:


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