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OfflineMetty
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Registered: 11/01/23
Posts: 16
Last seen: 6 days, 15 hours
Re: Recognizing and dealing with contamination [Re: Genome794]
    #28525273 - 11/01/23 08:51 AM (2 months, 26 days ago)

Hi there!
Is there anyone that could give me some advice on how to save this tub?
The strain is PES.H , agar to grain 28 days ago, grain to sub 15 days ago. Colonization was aggressive, Pic 1 is taken just 2 days after g2s. As u see The mycelium was strange so I didn’t open the tun until 3 days ago and noticed the contamination. It smells like bitter almonds but I don’t know i am a noob! Maybe it’s bacterial?! I marked the area but it seems the contamination didn’t expand from 3 days ago.

Is there any way to rescue the tub? How to deal with overlay and contamination?


*(I should add that possibly the tub has been  too wet. There were condensation on walls and lid . And water droplets on surfaces of substrate that i removed by a clean towel paper.)



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OfflineMetty
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Registered: 11/01/23
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Re: Recognizing and dealing with contamination [Re: Metty]
    #28525284 - 11/01/23 09:04 AM (2 months, 25 days ago)

Update: ⬆️
I decided to cut out the suspicious area. Is this any better?


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OfflineSmellyhobbit
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Re: Recognizing and dealing with contamination [Re: Metty]
    #28525291 - 11/01/23 09:14 AM (2 months, 25 days ago)

Truthfully probably not. May as well keep it around to see what happens. Seeing contam on one part of your surface doesn’t mean that’s the only part of the substrate it’s in. The entire block is contaminated. If you see contam on the surface before pins, it’s best to move on to a new project. If you see contam after pinning has begun, you can sometimes get a small flush out but it’s no guarantee.

Personally I’d toss that. If you want to hold onto it to see what happens to for it.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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OfflineMetty
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Registered: 11/01/23
Posts: 16
Last seen: 6 days, 15 hours
Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #28525298 - 11/01/23 09:22 AM (2 months, 25 days ago)

Yeah, I am kind of short on mushroom and a new project would take too lang! ;( so need to get at least one flush out of this.

Do you have any suggestions for dealing with overlay ?


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InvisibleMycolorado
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Re: Recognizing and dealing with contamination [Re: Metty] * 1
    #28525349 - 11/01/23 10:26 AM (2 months, 25 days ago)

That’s toast.


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OfflineAspectOfTheCreator
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Re: Recognizing and dealing with contamination [Re: Mycolorado]
    #28525365 - 11/01/23 10:44 AM (2 months, 25 days ago)

Toasty!


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OfflineSmellyhobbit
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Re: Recognizing and dealing with contamination [Re: Mycolorado] * 1
    #28525366 - 11/01/23 10:44 AM (2 months, 25 days ago)

Quote:

Mycolorado said:
That’s toast.



This is the truth. Expect nothing from that tub and start preparing your next project. It’s a bummer, but you’re almost definitely not getting anything from that leftover chunk.

Start your next project today and you’ll be as close to shrooms as you can be


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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OfflineKinoko314
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit] * 1
    #28525466 - 11/01/23 12:21 PM (2 months, 25 days ago)

Your chances probably aren't great, but if you don't have grain spawn ready to replace the tub then it doesn't matter if you keep it around.

You don't have overlay.  It looks matted down from repeated wet/dry cycles.


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Offlinehazyhorse
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Re: Recognizing and dealing with contamination [Re: Metty]
    #28525496 - 11/01/23 12:47 PM (2 months, 25 days ago)

:whathesaid:

im sure the spawn used wasn’t super clean either, just based on how you said the sub smelled.

you mentioned agar, i hope you’re using an SAB too! maybe try some modified tubs next time you go for a larger mono. ime the big unmodded tubs don’t allow great FAE but i know they work for other people

you actually do want water droplets on the surface (condensation on the side is irrelevant but shouldn’t hurt anything itself) & you want your sub to look like this at all times after 100%

there’s a lot of stuff that could have led to such poor results so make sure to really go over each part of your process to diagnose what is adding to the failure


--------------------
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OfflineMetty
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Registered: 11/01/23
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Re: Recognizing and dealing with contamination [Re: hazyhorse]
    #28525742 - 11/01/23 06:07 PM (2 months, 25 days ago)

Thank you all for your input and advise. 🤟
I just PC a new corn bag tonight. Crossing my fingers for success on the new round.

@hazyhorse now that you mention this. I recall that a very small area at the bottom of the bag was not colonized and was wet. But absolutely no bad smell from the spawn.
Yes i do have a SAB. But inoculation of spawn bag not possible in my SAB. So i do it above a preheated oven with  its door partially open.


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Offlinehazyhorse
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Re: Recognizing and dealing with contamination [Re: Metty]
    #28526038 - 11/01/23 10:54 PM (2 months, 25 days ago)

Quote:

Metty said:
Thank you all for your input and advise. 🤟
I just PC a new corn bag tonight. Crossing my fingers for success on the new round.

@hazyhorse now that you mention this. I recall that a very small area at the bottom of the bag was not colonized and was wet. But absolutely no bad smell from the spawn.
Yes i do have a SAB. But inoculation of spawn bag not possible in my SAB. So i do it above a preheated oven with  its door partially open.




yeah fuck that oven bullshit lol. it doesn’t work i promise. if you’re already using agar just use wedges to grain jars. no reason to be using a pressure cooker & agar but still injecting bags or whatever. jars inoculated with agar wedges are the way to go unless you take super special consideration in your SAB with bags if you’re using wedges. look into making jars of grain, i promise it’s better than bags for what you’re currently attempting 

smell is probably the worst indicator for contam imo. if it smells bad, it probably looks worse. uncolonized grains & weird wet spots are a huge sign of contam in colonizing grains

tbh i would ditch the corn & use a more standard grain like wheat, rye, millet, or WBS but if the corn is hydrated properly it should be fine. just a tricky grain to work with compared to the others

stick with it homie :thumbup:


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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Offlineloc dog
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Registered: 09/07/23
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Last seen: 22 days, 11 hours
Re: Recognizing and dealing with contamination [Re: hazyhorse]
    #28529766 - 11/04/23 11:53 PM (2 months, 22 days ago)

I have an LC of cambodian and the surface is one solid white blob about 1/8th inch thick. Is there any chance it is not mold??? Should have taken picture, but shook up and put on stirrer. Will put a few drops from each questionable one on agar and see what grows. The LC is 1 part LME 4 parts corn sugar, and 1/3 gram peptone per LC jar. They were stirred magnetically every 2 days, then was busy and 4 days later this happened.


Edited by loc dog (11/04/23 11:57 PM)


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Offlinescwoopz
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Re: Recognizing and dealing with contamination [Re: loc dog]
    #28531126 - 11/05/23 08:02 PM (2 months, 21 days ago)

Hey now! Here is an APE shoebox of mine going right now. It seems like there might be two different mycelium-looking things in the box. One is a bit thinner than the other, which seems thicker. But both look white and could be the same? I don’t notice any particular shells. Do you have any thoughts or recommendations? Sorry the pictures aren’t perfect—I can get better ones tomorrow in the sunlight! Thank you!



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OfflineKinoko314
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Re: Recognizing and dealing with contamination [Re: scwoopz]
    #28531213 - 11/05/23 09:12 PM (2 months, 21 days ago)

I'm not sure if they are the same mycelium or not, but it's a worrying sign that there is so much growth on the surface in some places, and none in others.

Whether it's bacteria or competing mycelium (mold), I think it's pretty contaminated.  Let it ride for now, but keep expectations low.


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OfflineTomShruise
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Registered: 01/03/14
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Re: Recognizing and dealing with contamination [Re: Kinoko314]
    #28531349 - 11/06/23 04:43 AM (2 months, 21 days ago)

Hi. Im trying to figure out why i dont have growth on one side of this cake. Could this be contamination? It looks like a thin veil covering the substrate. Im aware they need more fae which im trying to incorporate.

I should also note, this bag was left too long before i went to fruiting and they started pinning before hand.

Any info would be much appreciated.

Thanks




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Offlinescwoopz
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Re: Recognizing and dealing with contamination [Re: Kinoko314]
    #28531359 - 11/06/23 05:15 AM (2 months, 21 days ago)

Quote:

Kinoko314 said:
I'm not sure if they are the same mycelium or not, but it's a worrying sign that there is so much growth on the surface in some places, and none in others.

Whether it's bacteria or competing mycelium (mold), I think it's pretty contaminated.  Let it ride for now, but keep expectations low.



Thanks! Cool, that makes sense to me. It’s kinda what I expected from these jars.

Btw, I have these this shoebox (and one other that looks silar) about 4ft lower than my others. They all have lids in and snapped shut. Should I isolate the contaminated ones to a different closet area, or (given that these are all closed) is it alright? The other boxes spawned a week earlier and are basically fully colonized, I’m waiting for pinning now


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OfflineSmellyhobbit
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Re: Recognizing and dealing with contamination [Re: TomShruise] * 1
    #28531384 - 11/06/23 06:15 AM (2 months, 21 days ago)

Quote:

TomShruise said:
Hi. Im trying to figure out why i dont have growth on one side of this cake. Could this be contamination? It looks like a thin veil covering the substrate. Im aware they need more fae which im trying to incorporate.

I should also note, this bag was left too long before i went to fruiting and they started pinning before hand.

Any info would be much appreciated.

Thanks






You’re not guaranteed growth all over a cake. That’s just where it wants to fruit


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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OfflineKinoko314
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Re: Recognizing and dealing with contamination [Re: scwoopz]
    #28531441 - 11/06/23 07:41 AM (2 months, 21 days ago)

Quote:

scwoopz said:
Quote:

Kinoko314 said:
I'm not sure if they are the same mycelium or not, but it's a worrying sign that there is so much growth on the surface in some places, and none in others.

Whether it's bacteria or competing mycelium (mold), I think it's pretty contaminated.  Let it ride for now, but keep expectations low.



Thanks! Cool, that makes sense to me. It’s kinda what I expected from these jars.

Btw, I have these this shoebox (and one other that looks silar) about 4ft lower than my others. They all have lids in and snapped shut. Should I isolate the contaminated ones to a different closet area, or (given that these are all closed) is it alright? The other boxes spawned a week earlier and are basically fully colonized, I’m waiting for pinning now




You don't have to worry about isolating sketchy tubs.  It shouldn't affect your other tubs.


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OnlineSupaThaRipper
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Registered: 09/02/13
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #28531628 - 11/06/23 11:30 AM (2 months, 20 days ago)

Quote:

Kizzle said:
Metabolites - Although a large amount of metabolites often indicates contamination, small amounts are common on fully colonized substrates and spawn/PF jars. Metabolites are normally yellow but red metabolites sometimes occur in spawn jars. Metabolites may also appear as a yelllow discoloration of the mycelium.







Ridiculous amount of metabolites indicating contamination?
Another type of contamination all together?
Good or belongs in the trash?


Edited by SupaThaRipper (11/06/23 11:31 AM)


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OfflineFree Guy
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Registered: 11/06/23
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Re: Recognizing and dealing with contamination [Re: SupaThaRipper]
    #28531649 - 11/06/23 11:44 AM (2 months, 20 days ago)

Hey, first post to this forum; sorry if I'm jumping into the wrong place here.

My first monotub! These are Natalensis; I haven't had a huge flush, just a few fruits per day for a couple of weeks now.

This morning, I see hitchhikers on the fruits!

I pulled these, and these fuzzy growths seemed to exist wholly in the damp sheen on the top of the fruit; they wiped right off in a light rinse.

Can these fruits be kept?

Is this monotub compromised?

Thanks for any feedback!






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