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Offlinecozmyc
gentle modern ape
I'm a teapot User Gallery

Registered: 06/20/21
Posts: 2,131
Last seen: 1 day, 1 hour
Re: Recognizing and dealing with contamination [Re: Genome794]
    #28504024 - 10/14/23 05:52 AM (3 months, 13 days ago)

Yes bacterial, but you didn't transfer it with scissors, it's was likely in your spawn. Clean healthy spawn has a great immune system.


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You're conscious population 2 stardust
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and that's valuable :sunny:


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OfflineCorey
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Registered: 02/28/22
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Last seen: 1 month, 20 days
Re: Recognizing and dealing with contamination [Re: cozmyc] * 1
    #28504047 - 10/14/23 07:23 AM (3 months, 13 days ago)

Thank you so much. Truly grateful for you.


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Offlinescwoopz
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Registered: 01/27/23
Posts: 82
Loc: Fennario
Last seen: 2 days, 12 hours
Re: Recognizing and dealing with contamination [Re: Corey]
    #28506779 - 10/16/23 11:43 AM (3 months, 10 days ago)

Hello! I was wondering if it looks like my quart jar has some contamination in it. Some of the areas look a bit extra fuzzy compared to the mycelium, but I’m struggling to tell if that is the fuzzy edge of the mycelium or a more cobweb-y mold. I could try spawning this jar regardless—it’ll be ready in a few days!

I tossed two from contamination already. I went from a spore syringe right to grain because… mostly laziness. I had no contam last time (my first grow), but I used pint jars instead of quarts—I think the extra amount of time it takes to colonize a quart from a spore syringe has been part of my contamination issues. I’ll definitely be doing agar next time but luckily have this jar (maybe contaminated) and four other that’s look good! Let me know what you all think.




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InvisibleSmartattack
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Registered: 12/21/18
Posts: 3,775
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Re: Recognizing and dealing with contamination [Re: scwoopz]
    #28506907 - 10/16/23 01:41 PM (3 months, 10 days ago)

Spore to grain isn't an issue because of time involved,  it's because they are dirty.

Oats that look like that never pan out well for me but they look better than I would expect. If it stays white then it's probably suitable to fruit in a shoebox at least.


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* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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Offlinescwoopz
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Registered: 01/27/23
Posts: 82
Loc: Fennario
Last seen: 2 days, 12 hours
Re: Recognizing and dealing with contamination [Re: Smartattack]
    #28506926 - 10/16/23 01:57 PM (3 months, 10 days ago)

Quote:

Smartattack said:
Spore to grain isn't an issue because of time involved,  it's because they are dirty.

Oats that look like that never pan out well for me but they look better than I would expect. If it stays white then it's probably suitable to fruit in a shoebox at least.




That makes sense about syringes being dirty—I was thinking along the lines of: there is probably always a chance of some other contamination getting in, even as they sit there colonizing for now a whole month, and even if the spores began clean, the longer they sit in a closet the higher the probability something gets in. Does that follow?

On my last grow, I did spores into pint jars of brown rice. I sterilized those (and my oats) for 2hr at 15psi. Could the brown rice have helped in any way? Thank you for helping educate me!


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Offlinewelho
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Registered: 08/30/23
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Last seen: 1 day, 18 hours
Re: Recognizing and dealing with contamination [Re: scwoopz]
    #28507791 - 10/17/23 07:14 AM (3 months, 10 days ago)



Contamination?


Edited by welho (10/21/23 06:18 PM)


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InvisibleMicroMycelium
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Registered: 06/06/23
Posts: 461
Loc: Probably at work
Re: Recognizing and dealing with contamination [Re: welho]
    #28507804 - 10/17/23 07:32 AM (3 months, 10 days ago)

So I had this slimy jar…
It looked great at 100% but I left it sitting for 3 weeks over which it shrank and produced slimy opaque metabolites that were pooling on the bottom.
This was fully “recovered” from shaking it again after I noticed that.



I spawned it at a 1:2 ratio and it fruits just fine.



Just wanted to post this to say metabolites don’t always mean your grain isn’t viable… IMO/E it’s more important to track the homogeneity of the mycelium development.


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OfflineNichrome
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Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
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Re: Recognizing and dealing with contamination [Re: Smartattack]
    #28508219 - 10/17/23 02:18 PM (3 months, 9 days ago)

Quote:

Smartattack said:
Spore to grain isn't an issue because of time involved,  it's because they are dirty.

Oats that look like that never pan out well for me but they look better than I would expect. If it stays white then it's probably suitable to fruit in a shoebox at least.





Very good to see you.

:hatsoff:


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineKuroKitsune
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Registered: 11/05/22
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Re: Recognizing and dealing with contamination [Re: Nichrome]
    #28508345 - 10/17/23 04:04 PM (3 months, 9 days ago)

Quote:

Smartattack said:
Spore to grain isn't an issue because of time involved,  it's because they are dirty.



This! Theoretically, even if they were clean from completely sterile conditions through every process, a good filter like SFDs (synthetic filter discs) shouldn't let spores through. However, if you used polyfil and didn't pack it tightly enough, or used tyvek and PC'd it multiple times, then they'd be much more likely to contaminate, especially during a shake or if left somewhere with airflow. As long as your filters are okay, the only thing you should be concerned about is using agar next time. :smile:


Edited by KuroKitsune (10/17/23 04:20 PM)


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OfflineRas_Irie
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Registered: 07/30/15
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Re: Recognizing and dealing with contamination [Re: KuroKitsune]
    #28512495 - 10/21/23 01:09 AM (3 months, 6 days ago)

Hello, im in confusion, started from spores, than there was some pins, i made a clone dish, but the growing is strange. Should I do more teansferes or its contamination? Getting bacteria in grains and trich in tubs.







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OfflineKinoko314
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Registered: 12/16/22
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Re: Recognizing and dealing with contamination [Re: Ras_Irie]
    #28512828 - 10/21/23 10:20 AM (3 months, 5 days ago)

First one looks really good.

Second one looks good, but I'd transfer once to be safe.

Last one looks a bit rough.  I would continue transferring.


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InvisibleBigwormS
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Registered: 03/19/22
Posts: 1,868
Loc: Murica
Re: Recognizing and dealing with contamination [Re: Kinoko314]
    #28514755 - 10/22/23 09:29 PM (3 months, 4 days ago)

I sent the left one to a shoebox. Right one looked a little thick and failed the smell test. It was a little sweet and funky. Both came from the same agar plate but I think I did something wrong transferring the agar to this one.



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OfflineNichrome
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Registered: 12/17/18
Posts: 6,486
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Re: Recognizing and dealing with contamination [Re: Bigworm] * 1
    #28515543 - 10/23/23 05:16 PM (3 months, 3 days ago)

You fucked it up. Operator error.


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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InvisibleBigwormS
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Registered: 03/19/22
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Loc: Murica
Re: Recognizing and dealing with contamination [Re: Nichrome] * 1
    #28515596 - 10/23/23 05:54 PM (3 months, 3 days ago)

Quote:

Nichrome said:
You fucked it up. Operator error.



Oh, without a doubt. I'm just posting to show newer cultivators just cause 1 jar looks good from the same plate doesn't mean they are all going to be clean if you fuck up somewhere.


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OfflineCadreamin
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Registered: 10/22/23
Posts: 5
Last seen: 3 months, 1 day
Re: Recognizing and dealing with contamination [Re: Bigworm]
    #28515634 - 10/23/23 06:14 PM (3 months, 3 days ago)

Hi all, trying to confirm if this is some form of contamination or bruising. I've got mycelium turning blue, some pins that seem to have aborted/are turning darker. This is PE and my first grow. Any advice would be fantastic.








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OfflineNichrome
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Registered: 12/17/18
Posts: 6,486
Loc: Zone 5
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Re: Recognizing and dealing with contamination [Re: Bigworm]
    #28515636 - 10/23/23 06:16 PM (3 months, 3 days ago)

Quote:

Bigworm said:
Quote:

Nichrome said:
You fucked it up. Operator error.



Oh, without a doubt. I'm just posting to show newer cultivators just cause 1 jar looks good from the same plate doesn't mean they are all going to be clean if you fuck up somewhere.





Me too, from the narrator seat.

:highfive1:


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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InvisibleHILLBILLY OUTLAWS
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Registered: 04/21/22
Posts: 2,852
Loc: Luckenbach Texas
Re: Recognizing and dealing with contamination [Re: Nichrome]
    #28515727 - 10/23/23 07:10 PM (3 months, 3 days ago)

Quote:

Nichrome said:
Quote:

Bigworm said:
Quote:

Nichrome said:
You fucked it up. Operator error.



Oh, without a doubt. I'm just posting to show newer cultivators just cause 1 jar looks good from the same plate doesn't mean they are all going to be clean if you fuck up somewhere.





Me too, from the narrator seat.

:highfive1:





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Offlinecowlickcade
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Registered: 04/30/23
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #28520611 - 10/27/23 09:00 PM (2 months, 30 days ago)

Hey guys, so I'm not too sure what to think about this, I ordered and received a l.c. of starry night zand when I tested on agar it looked good at first so as soon as I could see some decent growth I transfered just in case. But today I looked at the original plate again and it looks like this? I obviously theree Is a contam, but I honestly can't tell if it's even mycelium now. It just looks off to me... the transfer plate looks weird to , almost fuzzy like..



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OfflineJXAllen
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Registered: 08/06/21
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Re: Recognizing and dealing with contamination [Re: cowlickcade] * 2
    #28520616 - 10/27/23 09:03 PM (2 months, 30 days ago)

That's mold.


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Cultures I'm workin on


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OfflineSmellyhobbit
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Registered: 04/01/22
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Re: Recognizing and dealing with contamination [Re: JXAllen] * 1
    #28520622 - 10/27/23 09:10 PM (2 months, 30 days ago)

Very happy mold though.


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A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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