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javazqq
Stranger

Registered: 03/18/23
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Loc: Oaxaca, Mexico
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Re: Recognizing and dealing with contamination [Re: Kinoko314]
#28464444 - 09/10/23 02:33 PM (4 months, 15 days ago) |
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Hi there, newbies here, after a few successful yields our beginners luck has run out, now every tub goes green before first flush or right after it, we've done some agar plates from spores but mycelium looks fairly odd, we want to do some transfers but want to know your opinion on our plates, thank you very much



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Kinoko314
Stranger Danger



Registered: 12/16/22
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Re: Recognizing and dealing with contamination [Re: javazqq]
#28464704 - 09/10/23 07:47 PM (4 months, 15 days ago) |
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Wow, those look pretty bad. Usually you would do a transfer before it grows all the way out to the edge.
I'd start over with the spores on agar if I were you. Make sure you only use a drop or two.
Edited by Kinoko314 (09/10/23 07:47 PM)
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SeizureSalad
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Re: Recognizing and dealing with contamination [Re: javazqq]
#28465013 - 09/11/23 04:06 AM (4 months, 15 days ago) |
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As a fellow beginner, I dread the day that my luck runs out
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Bustaboo
Calmer than you are


Registered: 07/17/23
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Re: Recognizing and dealing with contamination [Re: SeizureSalad]
#28465214 - 09/11/23 10:04 AM (4 months, 14 days ago) |
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Looks like the myc is surrounded by bacteria on all the plates. Kinoko is right about starting a new plate, but I would also try to take a transfer from that first plate between the bacterial edge and the middle and see what it does. It should grow faster than a germination plate and it will give you something to do while waiting on your new germ plate.
-------------------- Looking for Yeti and Ghost spores.
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sven137
Noob


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Re: Recognizing and dealing with contamination [Re: Kizzle]
#28465758 - 09/11/23 07:26 PM (4 months, 14 days ago) |
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I think I have an infection. I planned on moving away from spores via purchased syringe, this further solidifies that. I only do Shoe Boxes, and this is not the first time I've seen this. I still have 4 other boxes that are infection free, for now. What surprised me was how fast this grew. Less than 48hrs ago this box looked great. Then I peeked in and saw this after 2days. Trich, pen? What is it do you think?
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sven137
Noob


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Re: Recognizing and dealing with contamination [Re: sven137]
#28465759 - 09/11/23 07:28 PM (4 months, 14 days ago) |
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My wife and I scooped that out and ate it with Greek yogurt. Not really.
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cozmyc
gentle modern ape


Registered: 06/20/21
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Re: Recognizing and dealing with contamination [Re: sven137]
#28465766 - 09/11/23 07:33 PM (4 months, 14 days ago) |
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Lol. Definitely Trich. Bright white to green gives it away. You're only 11 posts away from good genetics. Or 
Edit: that's weird I thought I read your posts @ 39... so 36 to go
-------------------- You're conscious population 2 stardust ---------------------- and that's valuable
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sven137
Noob


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Re: Recognizing and dealing with contamination [Re: cozmyc]
#28465774 - 09/11/23 07:41 PM (4 months, 14 days ago) |
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I thought it was Trich, but I don't have the eyes quite yet. That's the first explosion like that I've seen now that I think about it. The other times were spots here and there, and I would try and scoop it out. Ha! I learned pretty quick that stuff can't be compartmentalized. It is the Borg, resistance is futile...
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FungusFan
Mad Scientist



Registered: 04/10/23
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Re: Recognizing and dealing with contamination [Re: sven137]
#28466349 - 09/12/23 10:58 AM (4 months, 13 days ago) |
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I've been having problems with trich, not in my tubs thank God but my petri's. I do everything I can to keep very sterile but that trich is sneaky. I use 10% bleach/ water on everything, scrub my hands and under fingernails, flame sterilize. I've even went as far as to wipe the whole room down with bleach/ water, I have an HEPA 13 air purifier in the clean room and another just outside the door and I turn it off 20 mins before any transfer, trich is frustrating.
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PBJ710
Strangler


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Re: Recognizing and dealing with contamination [Re: FungusFan] 1
#28466391 - 09/12/23 11:47 AM (4 months, 13 days ago) |
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Are you saying that you have a laminar flow hood with an appropriate H13 filter AND properly matched blower or just that you have a household air filter running in the room while doing open air transfers? Pictures of your setup and plates would greatly help.
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FungusFan
Mad Scientist



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Re: Recognizing and dealing with contamination [Re: PBJ710]
#28466467 - 09/12/23 12:57 PM (4 months, 13 days ago) |
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I wish I had a flow hood, just a little air purifier that I run for around 30 minutes before the transfers. I shut it off 20 minutes before I do transfers to let the air settle and do my work in a SAB that I have wiped down with 70% ISO. I'm thinking it might be the fan in the room next door and when I open the door to my clean room some nasties get in.
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FungusFan
Mad Scientist



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Re: Recognizing and dealing with contamination [Re: FungusFan]
#28466478 - 09/12/23 01:10 PM (4 months, 13 days ago) |
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cozmyc
gentle modern ape



Registered: 06/20/21
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Re: Recognizing and dealing with contamination [Re: FungusFan] 2
#28466508 - 09/12/23 01:43 PM (4 months, 13 days ago) |
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I suggest getting a rack that fits the bottom of the tub, to raise your working surface up. Mine would always move around too much though, so I built a little frame.
-------------------- You're conscious population 2 stardust ---------------------- and that's valuable
Edited by cozmyc (09/12/23 04:17 PM)
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PBJ710
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Re: Recognizing and dealing with contamination [Re: cozmyc] 3
#28466558 - 09/12/23 02:16 PM (4 months, 13 days ago) |
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I would also flip the tub over so you don't have the gap around the lid above your working area - maybe even use a dampened towel on the bottom of the tub to help contain any contams that fall out of the air. The lid may be snapped closed, but it still allows some airflow to move under it and thats bad when you need still air to maintain cleanliness. Make sure you're not bumping into the hand holes while working on things in it as well.
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FungusFan
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Re: Recognizing and dealing with contamination [Re: PBJ710] 1
#28466570 - 09/12/23 02:25 PM (4 months, 13 days ago) |
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I've actually thought about a rack and flipping the tub. I do spray the inside with ISO, wipe it out and spray ISO again and leave it to trap some contams. I'll try both suggestions and see if it makes a difference. I think another possibility is I got a bad batch of dishes, the only times I've gotten trich is with this sleeve of dishes. I never had a problem when I was using 1/4 pint jars for agar dishes. I'll keep trying, mistakes are good teachers. Thank you for your input.
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xVadisx
MountainsDogsAndFungi



Registered: 09/03/21
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Re: Recognizing and dealing with contamination [Re: FungusFan] 2
#28466985 - 09/12/23 08:59 PM (4 months, 13 days ago) |
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Quote:
FungusFan said: I've actually thought about a rack and flipping the tub. I do spray the inside with ISO, wipe it out and spray ISO again and leave it to trap some contams. I'll try both suggestions and see if it makes a difference. I think another possibility is I got a bad batch of dishes, the only times I've gotten trich is with this sleeve of dishes. I never had a problem when I was using 1/4 pint jars for agar dishes. I'll keep trying, mistakes are good teachers. Thank you for your input.
Still Air Boxes are for still air -- not clean air. Yes, we are all going for clean in them, but mostly by using slow movements. So, I would suggest not worrying about the ISO as much. At least not the spray again part, or even really any of it. I wouldn't overthink the root cause too much and would move forward! The suggestions to flip and raise your work surface are solid +1 and +1 (love the frame there!). In any case, a good few sprays with some lightly soapy water on the sides works wonders.
Someone posted on the rate air settles, or the rate different sized contaminants settle in still air. It takes a long time. Like...much much longer than 20 minutes. So, I just wouldn't worry so much about the air settling either so long as you don't have a fan blowing air around the room while you're working. I also have a HEPA filter running in the house, but turn the thing off for myco work because it'll just create turbulance and ultimately the spore load in the air....don't get me started too much, and even though I use the things, it's kind of like putting a clean ice cube in a glass of dirty water. It helps, but only so much 
You've got this!
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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MicroMycelium
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Registered: 06/06/23
Posts: 461
Loc: Probably at work
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Re: Recognizing and dealing with contamination [Re: xVadisx]
#28471439 - 09/16/23 05:44 PM (4 months, 9 days ago) |
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My second tub where this appeared. Don’t think it’s trich.

I’ve got three quarts of spawn in here Should I put salt paste on the contam? Last tub fruited just fine but I tossed it in the compost after the flush.
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AspectOfTheCreator
Mastering the Art of Success



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Re: Recognizing and dealing with contamination [Re: MicroMycelium]
#28471455 - 09/16/23 05:55 PM (4 months, 9 days ago) |
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Jars finishing up. Good? Yay or nay
Edited by AspectOfTheCreator (09/16/23 05:55 PM)
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xVadisx
MountainsDogsAndFungi



Registered: 09/03/21
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Quote:
AspectOfTheCreator said:

Jars finishing up. Good? Yay or nay
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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SeizureSalad
Stranger


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Re: Recognizing and dealing with contamination [Re: xVadisx]
#28473560 - 09/18/23 10:03 AM (4 months, 7 days ago) |
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Well my friends, my first grow ever has just developed the mean green:

This monotub was seeded with grain spawn and coco coir as bulk substrate 12 days ago. I have 3 questions:
1. Do I throw away this monotub now, and if so how do I take care not to spread this green shit all over my apartment? 2. How long after seeding the monotub should I start seeing pins (assuming the tub is healthy) 3. Do you see any contamination on the left side of the image? The crap I see is all on the right side but I am very very new to this.
Thank you all for the help, I am very sad that my first grow ended up like this. 
edit: another picture:
Edited by SeizureSalad (09/18/23 10:07 AM)
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