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Trabin2836
Stranger
Registered: 10/07/22
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Last seen: 1 year, 2 months
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Re: Recognizing and dealing with contamination [Re: Rotnpins]
#27986666 - 10/07/22 04:42 PM (1 year, 5 months ago) |
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https://This is my first grow and it seems to have stalled a bit. The mycelium grew on rye and I mixed with a substrate of coco coir. A white fluffy substance grew pretty quickly shown in the pictures. It has stayed similar to this for about two weeks. I am fine with patiently waiting this out, but if there is some contamination here I would like to know.
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Smellyhobbit
Actual Retard
Registered: 04/01/22
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Re: Recognizing and dealing with contamination [Re: Trabin2836]
#27986670 - 10/07/22 04:44 PM (1 year, 5 months ago) |
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Quote:
Trabin2836 said: https://This is my first grow and it seems to have stalled a bit. The mycelium grew on rye and I mixed with a substrate of coco coir. A white fluffy substance grew pretty quickly shown in the pictures. It has stayed similar to this for about two weeks. I am fine with patiently waiting this out, but if there is some contamination here I would like to know.
No contamination that I can see, just dry sub.
Are you fanning or anything like that?
-------------------- A Love Letter to New Growers A Guide for New Growers Growth 2023 - A Year In Review Just because your tub contamed, doesn’t mean your attitude has to contam as well.
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Trabin2836
Stranger
Registered: 10/07/22
Posts: 8
Last seen: 1 year, 2 months
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#27986695 - 10/07/22 04:57 PM (1 year, 5 months ago) |
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I am doing good a small amount of fanning. Humidity, as shown on my cheap hygrometer is in the high 90% from the perlite below the tin foil base. Temperatures has been right around 70 degrees.
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: Kizzle] 1
#27986705 - 10/07/22 05:04 PM (1 year, 5 months ago) |
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My advice would be to not do any more fanning, trim the foil back to where it is only very slightly larger than the cake, and then mist the mycelium until it forms thousands of little beads all over its surface.
Fanning is outdated. The perlite can’t do it’s job because moisture isn’t evaporating up and around the cake. The foil should basically be almost the same size as the cake. And there are good guides here for proper surface conditions, but if it isn’t maintaining all those beads of moisture on its own then there’s too much free air exchange going on. Passive air is good enough. If you put the lid on your tub and it isn’t air tight, that’s good enough.
-------------------- A Love Letter to New Growers A Guide for New Growers Growth 2023 - A Year In Review Just because your tub contamed, doesn’t mean your attitude has to contam as well.
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Trabin2836
Stranger
Registered: 10/07/22
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Last seen: 1 year, 2 months
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit] 1
#27986739 - 10/07/22 05:28 PM (1 year, 5 months ago) |
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I will try that, thank you for the advice.
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Smellyhobbit
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Re: Recognizing and dealing with contamination [Re: Trabin2836]
#27986756 - 10/07/22 05:34 PM (1 year, 5 months ago) |
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Quote:
Trabin2836 said: I will try that, thank you for the advice.
Good luck! Keep us updated!
-------------------- A Love Letter to New Growers A Guide for New Growers Growth 2023 - A Year In Review Just because your tub contamed, doesn’t mean your attitude has to contam as well.
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Bigworm
Registered: 03/19/22
Posts: 2,010
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#27986771 - 10/07/22 05:42 PM (1 year, 5 months ago) |
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KabutoFShroomelo
vice cpt
Registered: 09/27/22
Posts: 1
Loc: Hidden Mush Village
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Re: Recognizing and dealing with contamination [Re: Bigworm]
#27986974 - 10/07/22 07:17 PM (1 year, 5 months ago) |
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Hello, first of all Thanks! For all the pics and infos.
First spawm i hav made...
Grain: Whole Brown Rice ->Still Air Box -> Spore sering (p.cubes equador?) -> 10 days after
Cobweb?
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Nichrome
I'm a torso!
Registered: 12/17/18
Posts: 6,764
Loc: Zone 5
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Re: Recognizing and dealing with contamination [Re: Trabin2836]
#27987045 - 10/07/22 07:49 PM (1 year, 5 months ago) |
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Quote:
Trabin2836 said: https://This is my first grow and it seems to have stalled a bit. The mycelium grew on rye and I mixed with a substrate of coco coir. A white fluffy substance grew pretty quickly shown in the pictures. It has stayed similar to this for about two weeks. I am fine with patiently waiting this out, but if there is some contamination here I would like to know.
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“Tea is the magic key to the vault where my brain is kept.” – Frances Hardinge
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Bigworm
Registered: 03/19/22
Posts: 2,010
Loc: Murica
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Quote:
KabutoFShroomelo said: Hello, first of all Thanks! For all the pics and infos.
First spawm i hav made...
Grain: Whole Brown Rice ->Still Air Box -> Spore sering (p.cubes equador?) -> 10 days after
Cobweb?
It looks a bit wispy but let it grow a bit longer. Even if it's bacterial you can try top fruiting the jar and try to get a clone. You should have gone to agar first. Spore syringes are usually not contamination free. I have been lucky with getting a 50/50 failure rate going syringe to grain, usually it's a failure for most.
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larksky
Strangerthings
Registered: 10/04/22
Posts: 17
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Re: Recognizing and dealing with contamination [Re: Bigworm]
#27988676 - 10/08/22 04:41 PM (1 year, 5 months ago) |
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Swab (PESH) to agar 6 days ago. Any input on this one? Swabbed and then dunked the swab in agar. Anything looking suspicious?
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Bigworm
Registered: 03/19/22
Posts: 2,010
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: larksky]
#27988781 - 10/08/22 05:29 PM (1 year, 5 months ago) |
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Sorry to say but I'm not seeing anything there I would transfer. Maybe someone with more experience will see something I'm not.
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Nichrome
I'm a torso!
Registered: 12/17/18
Posts: 6,764
Loc: Zone 5
Last seen: 9 days, 20 hours
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Re: Recognizing and dealing with contamination [Re: larksky] 1
#27988784 - 10/08/22 05:31 PM (1 year, 5 months ago) |
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Quote:
larksky said: Swab (PESH) to agar 6 days ago. Any input on this one? Swabbed and then dunked the swab in agar. Anything looking suspicious?
Further transfers will allow what's there to really take form. None of that is mold.
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“Tea is the magic key to the vault where my brain is kept.” – Frances Hardinge
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Bigworm
Registered: 03/19/22
Posts: 2,010
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: Nichrome]
#27988791 - 10/08/22 05:33 PM (1 year, 5 months ago) |
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Quote:
Nichrome said:
Quote:
larksky said: Swab (PESH) to agar 6 days ago. Any input on this one? Swabbed and then dunked the swab in agar. Anything looking suspicious?
Further transfers will allow what's there to really take form. None of that is mold.
Damn, then I tossed a lot of good plates! 🤣
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Nichrome
I'm a torso!
Registered: 12/17/18
Posts: 6,764
Loc: Zone 5
Last seen: 9 days, 20 hours
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Re: Recognizing and dealing with contamination [Re: Bigworm] 2
#27988795 - 10/08/22 05:34 PM (1 year, 5 months ago) |
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A lot of people do.
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“Tea is the magic key to the vault where my brain is kept.” – Frances Hardinge
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Bigworm
Registered: 03/19/22
Posts: 2,010
Loc: Murica
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Re: Recognizing and dealing with contamination [Re: Nichrome]
#27988996 - 10/08/22 07:32 PM (1 year, 5 months ago) |
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So where would you transfer from on that plate? I feel like I wasted so much agar now.
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milkboy
Child
Registered: 05/01/21
Posts: 2,332
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Re: Recognizing and dealing with contamination [Re: Bigworm] 2
#27989516 - 10/09/22 03:58 AM (1 year, 5 months ago) |
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If you dont have an eye for it I'd suggest taking a transfer from each colony, watch them grow out and take notes.
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Nichrome
I'm a torso!
Registered: 12/17/18
Posts: 6,764
Loc: Zone 5
Last seen: 9 days, 20 hours
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Re: Recognizing and dealing with contamination [Re: milkboy] 1
#27989733 - 10/09/22 08:42 AM (1 year, 5 months ago) |
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--------------------
“Tea is the magic key to the vault where my brain is kept.” – Frances Hardinge
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Smellyhobbit
Actual Retard
Registered: 04/01/22
Posts: 12,058
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Last seen: 13 seconds
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#27989971 - 10/09/22 11:04 AM (1 year, 5 months ago) |
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I have two colonized jars left made with no prep grain. Some have contaminated, some have been perfectly fine. Roll of the dice. Just to sharpen my eye, is this bacterial? I see some moist grains mashed up against the glass here and there. No competing mold, which was visible on the previous jars. These might be good to send for a single flush, or I could top fruit them.
Opinions?
-------------------- A Love Letter to New Growers A Guide for New Growers Growth 2023 - A Year In Review Just because your tub contamed, doesn’t mean your attitude has to contam as well.
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Rotnpins
🤮 Rotten-Pins 🍄
Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit] 1
#27989975 - 10/09/22 11:05 AM (1 year, 5 months ago) |
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Quote:
Smellyhobbit said: I have two colonized jars left made with no prep grain. Some have contaminated, some have been perfectly fine. Roll of the dice. Just to sharpen my eye, is this bacterial? I see some moist grains mashed up against the glass here and there. No competing mold, which was visible on the previous jars. These might be good to send for a single flush, or I could top fruit them.
Opinions?
I'd top fruit it
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