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OnlineB Traven
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Re: Recognizing and dealing with contamination [Re: Hindsight] * 1
    #27935260 - 09/05/22 11:55 AM (1 year, 6 months ago)

Quote:

Hindsight said:
Intentional trich collision - for science:


On another note, I sometimes get plates like this and don't think I've ever seen anyone conclusively explain what this kind of strange pattern is (the consensus seems to be "whatever it is, just transfer away from it" - which works but doesn't satisfy my curiosity). I am starting to suspect it is bacteria. Might do an experiment to see if I can prove it.






I'd bet money that it's bacteria. Molds seem to go APESHIT on plates in my experience, and not just kick out slower-growing mystery colonies.

We're all trying to grow mushrooms here, and just get away from whatever isn't mushrooms. I know when I see funky shit in my plates, I just think "damn, I still suck at SAB work." And then spend a few minutes in my head geeking out about whether my hand motions need to be revised lol

That would be wild and fascinating if you went down the rabbithole of intentionally culturing your contaminants to investigate them further. That's the sort of stuff that public health agencies do to check for water-borne pathogens and whatnot.

If you do, I hope you share your techniques and results here...


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Beware of advice- even this.

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OfflineHindsight
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Re: Recognizing and dealing with contamination [Re: B Traven] * 1
    #27935396 - 09/05/22 01:27 PM (1 year, 6 months ago)

I'm doing trich experiments and already have learned a handful from it. I will be creating a thread on the results when I am done but have another month or so worth of tests to do before I'm finished.

And I agree with you about the bacteria - I think that is what the pattern is in the second plate. I cut a chunk out of the funny looking area and put it to LC and will wait to see if the LC becomes cloudy which will tell me whether or not bacteria is the cause.

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OfflineMandy cameron
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27935612 - 09/05/22 03:52 PM (1 year, 6 months ago)

Have big white fluffy cotton like white balls all over my cube, not sure if that is normal or should I worry

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Offlinecozmyc
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Re: Recognizing and dealing with contamination [Re: Mandy cameron]
    #27935729 - 09/05/22 04:49 PM (1 year, 6 months ago)

It's normal when there is not enough FAE.
Welcome to the forum!:mushroom2:


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You're conscious population 2 stardust
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OfflineRusty2096
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Re: Recognizing and dealing with contamination [Re: cozmyc]
    #27935923 - 09/05/22 06:45 PM (1 year, 6 months ago)

Quote:

cozmyc said:
It's normal when there is not enough FAE.
Welcome to the forum!:mushroom2:




Welcome!

Also, are they B+? Those are known to do that a lot.


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OfflineRotnpins
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Re: Recognizing and dealing with contamination [Re: Mandy cameron] * 1
    #27935950 - 09/05/22 07:02 PM (1 year, 6 months ago)

Quote:

Mandy cameron said:
Have big white fluffy cotton like white balls all over my cube, not sure if that is normal or should I worry




Welcome to the Shroomery :hi:

It's most likely either an indication that you need more FAE or a mutation like the white hats on B+ that were just mentioned... but honestly there is no way of knowing for sure without pictures.. please try to include an example picture when asking a question specifically about one of your grows.

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OfflineFethyl
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Re: Recognizing and dealing with contamination [Re: Rotnpins]
    #27937278 - 09/06/22 01:47 PM (1 year, 6 months ago)

Any thoughts y'all? My post got bumped off by some abnormally large pictures..


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InvisibleWyoMX
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Re: Recognizing and dealing with contamination [Re: Fethyl]
    #27937393 - 09/06/22 03:06 PM (1 year, 6 months ago)

There's nothing usable on that plate I'm afraid. I read your first post as well I'm not sure the answer though. Could definitely be your process but could also be your syringe that your using.

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Offlineechochild
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Re: Recognizing and dealing with contamination [Re: WyoMX]
    #27938308 - 09/07/22 08:10 AM (1 year, 6 months ago)

Hello all, newbie here. I've been searching online, and can't seem to find something on this.

I had an agar plate from a syringe, learned I used a little too much of the syringe for agar. Plate had 6 off white pearl looking splotches that I'm pretty sure were bacteria. One area looked like budding mycelium, so I took a small transfer as far away from the bacteria as I could and transferred to a new dish on 9/1.

What's growing on the plate looks white and fuzzy, and I don't really see any ropes. Curious what exactly it is that I'm growing here.

Also, the pic with the circle was for me trying to figure out how much it was growing day by day. I drew that circle 2 days ago. so it's advanced 2-3 mm since then on the 6 o'clock side. Sorry for the crappy pics, my camera didn't want to focus well.


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OnlineSmellyhobbitM
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Re: Recognizing and dealing with contamination [Re: Kizzle]
    #27938310 - 09/07/22 08:11 AM (1 year, 6 months ago)

Looks like tomentose mycelium to me, but I’m not the most experienced.


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A Guide for New Growers
Growth 2023 - A Year In Review

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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OfflineRotnpins
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit] * 2
    #27938317 - 09/07/22 08:16 AM (1 year, 6 months ago)

Quote:

Smellyhobbit said:
Looks like tomentose mycelium to me, but I’m not the most experienced.




:whathesaid:

The pictures aren't the best, but from what I can see it looks like tomentose mycelium..

Don't worry about chasing rhizomorphic growth (ropes).. tomentose growth is just as good.. people tend to focus on rhizo because it's easier to distinguish from certain contams, and because it looks pretty.. but clean growth is clean growth :super:

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Offlineechochild
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Re: Recognizing and dealing with contamination [Re: Rotnpins]
    #27938341 - 09/07/22 08:43 AM (1 year, 6 months ago)

Thanks both of you! I was wondering because it wasn't spreading super fast like I have seen on other contaminated dishes.

Do you think this would be ok to transfer small amounts to an LC to get growth ramped up quicker?

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OfflineRotnpins
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Re: Recognizing and dealing with contamination [Re: echochild]
    #27938359 - 09/07/22 08:53 AM (1 year, 6 months ago)

Slow growth is good growth..

Honestly I don't encourage anyone to send anything to LC unless their sterile technique and agar work are 100% on point... I'd recommend just working on your agar, sterile work, and patience.. mess around with LC later..

Saving a couple days won't matter if your grow contaminates.. so why add another contamination vector until you're absolutely confident in your work... just my 2 cents :shrug:

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Offlineechochild
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Re: Recognizing and dealing with contamination [Re: Rotnpins]
    #27938373 - 09/07/22 08:59 AM (1 year, 6 months ago)

Good point!

I have had about a 10% contamination rate on my agar thus far, but I have no clue if my sterile tech is to blame, or if it was that the syringes had contamination in them already.

All the plates of that strain ended up with those off white pearls I think were bacteria, so I sterilized those other plates because I saw no growth that looked even remotely like mycelium.

On my other plates of different strains I only had 1 that contaminated, but that tells I have room to improve.

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OfflineRusty2096
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Re: Recognizing and dealing with contamination [Re: echochild]
    #27938378 - 09/07/22 09:02 AM (1 year, 6 months ago)

Quote:

echochild said:
Thanks both of you! I was wondering because it wasn't spreading super fast like I have seen on other contaminated dishes.

Do you think this would be ok to transfer small amounts to an LC to get growth ramped up quicker?




Like they said it looks like myc but if you could upload a better picture it would help confirm it's not cobweb or some mold.

You can inoc many jars from a single plate. That being said, if you really want to get an LC going, I would suggest you to go ahead and make another transfer before. The reason for that is contam is sometimes really hard to see in LC and you want to make sure you will noc your LC with a very clean plate.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Offlineechochild
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Re: Recognizing and dealing with contamination [Re: Rusty2096]
    #27938400 - 09/07/22 09:14 AM (1 year, 6 months ago)

I don't know if these are any better/clearer. The growth is pure white, and the epicenter is from where I did the transfer.

My camera on my phone really doesn't want to focus on it, and I'm very hesitant to open it without being in the SAB where taking a pic would be very difficult.



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OnlineSmellyhobbitM
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Re: Recognizing and dealing with contamination [Re: echochild]
    #27938408 - 09/07/22 09:18 AM (1 year, 6 months ago)

Quote:

echochild said:
I don't know if these are any better/clearer. The growth is pure white, and the epicenter is from where I did the transfer.

My camera on my phone really doesn't want to focus on it, and I'm very hesitant to open it without being in the SAB where taking a pic would be very difficult.






Definitely do not open that plate outside of a sterile environment. The second pic there isn’t too bad, but it still looks like tomentose growth.

Most people don’t use plates before T2 or T3.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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Offlineechochild
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #27938420 - 09/07/22 09:25 AM (1 year, 6 months ago)

Quote:

Most people don’t use plates before T2 or T3.




T2 - T3 I assume means transfer 2 or transfer 3?

Edited by echochild (09/07/22 09:26 AM)

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OfflineRotnpins
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Re: Recognizing and dealing with contamination [Re: echochild]
    #27938427 - 09/07/22 09:30 AM (1 year, 6 months ago)

Quote:

echochild said:
Quote:

Most people don’t use plates before T2 or T3.




T2 - T3 I assume means transfer 2 or transfer 3?




Exactly... germination plate is T0, and every transfer is numbered from there T1>T2>T3, etc,..

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Offlineechochild
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Re: Recognizing and dealing with contamination [Re: Rotnpins]
    #27938431 - 09/07/22 09:32 AM (1 year, 6 months ago)

Quote:

Rotnpins said:
Quote:

echochild said:
Quote:

Most people don’t use plates before T2 or T3.




T2 - T3 I assume means transfer 2 or transfer 3?




Exactly... germination plate is T0, and every transfer is numbered from there T1>T2>T3, etc,..




I can see the wisdom in this. I'll skip the LC for now, take a small transfer to another couple of plates, and see how it goes.

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